Nahida Kh. Aliyeva, Durna R. Aliyeva, Saftar Y. Suleymanov, Fuad H. Rzayev, Eldar K. Gasimov, Irada M. Huseynova
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引用次数: 0
摘要
研究了盐胁迫对NaCl处理5 d玉米叶片叶肉(M)和束鞘(BS)叶绿体的影响。研究了不同盐度条件(0、100、200和250 mM NaCl)下植物的色素含量、77 K下的叶绿素荧光、光系统I和II的活性、多肽组成和类囊体膜的超微结构。盐处理导致PSII和PSI的荧光、光化学活性和类囊体蛋白含量降低。在高盐浓度下,M叶绿体F735/F686荧光比值降低,BS叶绿体F735/F686荧光比值升高。PSII光化学活性在两种叶绿体中均降低,但与对照相比,PSII光化学活性无统计学差异。对M和BS叶绿体类囊体膜蛋白组成的分析表明,PSII核心天线蛋白(47和43 kDa)和LHCII (28-24 kDa)在两种膜中均存在,但BS类囊体的含量要低得多。PSI核心68-kDa载脂蛋白的合成在M膜中受到抑制。类囊体的膜系统未见明显变化。盐胁迫对M叶绿体超微结构的影响大于对BS叶绿体超微结构的影响,并导致BS叶绿体形成粒状堆积。这些结果表明M和BS叶绿体对盐胁迫的响应不同。
Comparative Analysis of Mesophyll and Bundle Sheath Chloroplasts from Maize Plants Subjected to Salt Stress
The effect of salt stress was studied in mesophyll (M) and bundle sheath (BS) chloroplasts of maize plants treated with NaCl for 5 days. Pigment content, chlorophyll fluorescence at 77 K, activity of photosystems (PS) I and II, polypeptide composition, and ultrastructure of the thylakoid membranes were investigated in plants grown under different salinity conditions (0, 100, 200, and 250 mM NaCl). Salt treatment caused a decrease in the fluorescence, photochemical activity of PSII and PSI, and protein content of the thylakoids. At high salt concentrations, the F735/F686 fluorescence ratio was reduced in M chloroplasts and increased in BS chloroplasts. The photochemical activity of PSII was reduced in both types of chloroplasts, while no statistically significant difference was observed in the activity of PSI compared to the control. Analysis of the thylakoid membrane protein composition in the M and BS chloroplasts revealed that proteins of the PSII core antenna (47 and 43 kDa) and LHCII (28-24 kDa) were present in both types of membranes, but their content in the BS thylakoids was much lower. The synthesis of the PSI core 68-kDa apoprotein was suppressed in the M membranes. No noticeable changes were observed in the membrane system of BS thylakoids. Salt stress had a greater impact on the ultrastructure of M chloroplasts than on BS chloroplasts and caused formation of granal stacks in BS chloroplasts. These results indicate different response of the M and BS chloroplasts to salt stress.
期刊介绍:
Biochemistry (Moscow) is the journal that includes research papers in all fields of biochemistry as well as biochemical aspects of molecular biology, bioorganic chemistry, microbiology, immunology, physiology, and biomedical sciences. Coverage also extends to new experimental methods in biochemistry, theoretical contributions of biochemical importance, reviews of contemporary biochemical topics, and mini-reviews (News in Biochemistry).