Biochemistry (Moscow)最新文献_第3页

Determination of SARS-CoV-2 Main Protease (Mpro) Activity Based on Electrooxidation of Tyrosine Residue of a Model Peptide

IF 2.3 4区生物学

Biochemistry (Moscow) Pub Date : 2025-03-07 DOI: 10.1134/S0006297924603836

Tatiana A. Filippova, Rami A. Masamrekh, Tatiana E. Farafonova, Yulia Yu. Khudoklinova, Victoria V. Shumyantseva, Sergei A. Moshkovskii, Alexey V. Kuzikov

{"title":"Determination of SARS-CoV-2 Main Protease (Mpro) Activity Based on Electrooxidation of Tyrosine Residue of a Model Peptide","authors":"Tatiana A. Filippova, Rami A. Masamrekh, Tatiana E. Farafonova, Yulia Yu. Khudoklinova, Victoria V. Shumyantseva, Sergei A. Moshkovskii, Alexey V. Kuzikov","doi":"10.1134/S0006297924603836","DOIUrl":"10.1134/S0006297924603836","url":null,"abstract":"The proposed approach for determining catalytic activity of the SARS-CoV-2 main protease (Mpro) is based on registration of the peak area of electrochemical oxidation of tyrosine residue in the model peptide substrate CGGGAVLQSGY immobilized on the surface of a graphite screen-printed electrode (SPE) modified with gold nanoparticles (AuNP). The AuNP were obtained by electrosynthesis. Steady state kinetic parameters of Mpro in the reaction with the model peptide were determined: catalytic constant (kcat) was (3.1 ± 0.1) × 10−3 s−1; Michaelis constant (KM) was (358 ± 32) × 10−9 M; catalytic efficiency (kcat/KM) was 8659 s−1/M. The limit of detection (LOD) determined for Mpro using the proposed electrochemical system was 44 nM. The proposed approach is a promising tool to search for new Mpro inhibitors as drugs for treatment of coronavirus infections.","PeriodicalId":483,"journal":{"name":"Biochemistry (Moscow)","volume":"90 1","pages":"120 - 131"},"PeriodicalIF":2.3,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143570893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Portrait of Three Mammalian Bicistronic mRNA Transcripts, Derived from the Genes ASNSD1, SLC35A4, and MIEF1

IF 2.3 4区生物学

Biochemistry (Moscow) Pub Date : 2025-03-07 DOI: 10.1134/S0006297924603630

Dmitry E. Andreev, Ivan N. Shatsky

引用次数: 0

Energy-Dependent Non-Photochemical Quenching: PsbS, LhcSR, and Other Players

IF 2.3 4区生物学

Biochemistry (Moscow) Pub Date : 2025-03-07 DOI: 10.1134/S000629792460371X

Vasily V. Ptushenko, Andrei P. Razjivin

{"title":"Energy-Dependent Non-Photochemical Quenching: PsbS, LhcSR, and Other Players","authors":"Vasily V. Ptushenko, Andrei P. Razjivin","doi":"10.1134/S000629792460371X","DOIUrl":"10.1134/S000629792460371X","url":null,"abstract":"The photosynthetic apparatus of plants is capable of capturing even weak fluxes of light energy. Hence, strong and rapid increase in irradiance should be dangerous for plants. To solve the problems caused by fluctuations of incident radiation (up to excessive), plants have developed a number of protective mechanisms, including non-photochemical quenching (NPQ) of excited chlorophyll states. NPQ is a set of mechanisms that shorten the lifetime of excited chlorophyll states in the photosynthetic antenna, thereby reducing dangerous effects of light. The most rapid mechanism of NPQ is energy-dependent quenching (qE) triggered by the proton potential formation on the thylakoid transmembrane. The main molecular players of qE are xanthophylls (oxygen-containing carotenoids) and proteins of the thylakoid membrane: antenna component LhcSR in algae and mosses and photosystem II component PsbS in higher plants and some groups of “green lineage” alga. This review discusses molecular mechanisms of qE, with a special focus on the PsbS-dependent quenching. The discovery that PsbS does not bind pigments has led to the hypothesis of PsbS-dependent indirect activation of quenching, in which PsbS acts as a relay switching on the quenching sites in the major (LHCII) and/or minor photosynthetic antennae. The suggested mechanisms include the effect of PsbS on carotenoid conformation and/or pKa values of amino acid residues in PSII antennae. PsbS can also act as a membrane “lubricant” that ensures migration of the major antenna LHCII in the thylakoid membrane and its aggregation followed by transition to the quenched state.","PeriodicalId":483,"journal":{"name":"Biochemistry (Moscow)","volume":"90 1","pages":"44 - 60"},"PeriodicalIF":2.3,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143571000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Physiological Concentrations of Calciprotein Particles Trigger Activation and Pro-Inflammatory Response in Endothelial Cells and Monocytes

IF 2.3 4区生物学

Biochemistry (Moscow) Pub Date : 2025-03-07 DOI: 10.1134/S0006297924604064

Daria Shishkova, Victoria Markova, Yulia Markova, Maxim Sinitsky, Anna Sinitskaya, Vera Matveeva, Evgenia Torgunakova, Anastasia Lazebnaya, Alexander Stepanov, Anton Kutikhin

{"title":"Physiological Concentrations of Calciprotein Particles Trigger Activation and Pro-Inflammatory Response in Endothelial Cells and Monocytes","authors":"Daria Shishkova, Victoria Markova, Yulia Markova, Maxim Sinitsky, Anna Sinitskaya, Vera Matveeva, Evgenia Torgunakova, Anastasia Lazebnaya, Alexander Stepanov, Anton Kutikhin","doi":"10.1134/S0006297924604064","DOIUrl":"10.1134/S0006297924604064","url":null,"abstract":"Supraphysiological concentrations of calciprotein particles (CPPs), which are indispensable scavengers of excessive Ca2+ and PO43− ions in blood, induce pro-inflammatory activation of endothelial cells (ECs) and monocytes. Here, we determined physiological levels of CPPs (10 μg/mL calcium, corresponding to 10% increase in Ca2+ in the serum or medium) and investigated whether the pathological effects of calcium stress depend on the calcium delivery form, such as Ca2+ ions, albumin- or fetuin-centric calciprotein monomers (CPM-A/CPM-F), and albumin- or fetuin-centric CPPs (CPP-A/CPP-F). The treatment with CPP-A or CPP-F upregulated transcription of pro-inflammatory genes (VCAM1, ICAM1, SELE, IL6, CXCL8, CCL2, CXCL1, MIF) and promoted release of pro-inflammatory cytokines (IL-6, IL-8, MCP-1/CCL2, and MIP-3α/CCL20) and pro- and anti-thrombotic molecules (PAI-1 and uPAR) in human arterial ECs and monocytes, although these results depended on the type of cell and calcium-containing particles. Free Ca2+ ions and CPM-A/CPM-F induced less consistent detrimental effects. Intravenous administration of CaCl2, CPM-A, or CPP-A to Wistar rats increased production of chemokines (CX3CL1, MCP-1/CCL2, CXCL7, CCL11, CCL17), hepatokines (hepassocin, fetuin-A, FGF-21, GDF-15), proteases (MMP-2, MMP-3) and protease inhibitors (PAI-1) into the circulation. We concluded that molecular consequences of calcium overload are largely determined by the form of its delivery and CPPs are efficient inducers of mineral stress at physiological levels.","PeriodicalId":483,"journal":{"name":"Biochemistry (Moscow)","volume":"90 1","pages":"132 - 160"},"PeriodicalIF":2.3,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143570894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanisms of Antioxidant Protection of Low-Density Lipoprotein Particles Against Free Radical Oxidation

IF 2.3 4区生物学

Biochemistry (Moscow) Pub Date : 2025-03-07 DOI: 10.1134/S0006297924603381

Vadim Z. Lankin, Konstantin B. Shumaev, Valeria A. Medvedeva, Alla K. Tikhaze, Galina G. Konovalova

引用次数: 0

Rethinking the Evolutionary Origin, Function, and Treatment of Cancer

IF 2.3 4区生物学

Biochemistry (Moscow) Pub Date : 2025-03-07 DOI: 10.1134/S0006297924603575

Anatoly V. Lichtenstein

引用次数: 0

4-Methylumbelliferone, an Inhibitor of Hyaluronan Synthase, Prevents the Development of Oncological, Inflammatory, Degenerative, and Autoimmune Diseases

IF 2.3 4区生物学

Biochemistry (Moscow) Pub Date : 2025-03-07 DOI: 10.1134/S0006297924603459

Viktoriya V. Fedorova, Alexandra Tsitrina, Noreen Halimani, Yuri V. Kotelevtsev

{"title":"4-Methylumbelliferone, an Inhibitor of Hyaluronan Synthase, Prevents the Development of Oncological, Inflammatory, Degenerative, and Autoimmune Diseases","authors":"Viktoriya V. Fedorova, Alexandra Tsitrina, Noreen Halimani, Yuri V. Kotelevtsev","doi":"10.1134/S0006297924603459","DOIUrl":"10.1134/S0006297924603459","url":null,"abstract":"Hyaluronic acid (HA) is the main structure-forming polymer of the extracellular matrix. HA metabolism plays an important role in intercellular interaction in healthy organism and in various pathologies. HA is synthesized by hyaluronan synthase (HAS); mammals have three highly homologous isoforms of this enzyme: HAS1, HAS2, and HAS3. No highly specific competitive inhibitors of HASs have been described so far. 4-Methylumbelliferone (4-MU), a natural coumarin compound, is commonly used to inhibit HA synthesis in vivo and in cell cultures. The review is focused on the molecular mechanisms underlying the therapeutic effects of 4-MU and discusses results of 4-MU application in tissue cultures and animal disease models, as well as in first clinical trials of this compound. It was found that along with receptors and transcription factors, one of the pharmacological targets of 4-MU is HAS2, which is most common isoform of HAS. Moreover, it is inhibition of HA synthesis that underlies the pharmacological effects of 4-MU in oncological, autoimmune, degenerative, and hypercompensated regenerative processes (fibrosis, scar formation). New clinical drugs based on specific HAS2 inhibitors will be the first-in-class compounds to treat a wide range of diseases.","PeriodicalId":483,"journal":{"name":"Biochemistry (Moscow)","volume":"90 1","pages":"1 - 18"},"PeriodicalIF":2.3,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143570997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Induction of IMPDH-Based Cytoophidia by a Probable IMP-Dependent ARL13B–IMPDH Interaction 通过可能的imp依赖性ARL13B-IMPDH相互作用诱导基于impdh的嗜细胞现象

IF 2.3 4区生物学

Biochemistry (Moscow) Pub Date : 2025-01-17 DOI: 10.1134/S0006297924120162

Najva Alijani, Razieh Yazdanparast

{"title":"Induction of IMPDH-Based Cytoophidia by a Probable IMP-Dependent ARL13B–IMPDH Interaction","authors":"Najva Alijani, Razieh Yazdanparast","doi":"10.1134/S0006297924120162","DOIUrl":"10.1134/S0006297924120162","url":null,"abstract":"Inosine Monophosphate Dehydrogenase (IMPDH) catalyzes rate-limiting step of the reaction converting inosine monophosphate (IMP) to guanine nucleotides. IMPDH is up-regulated in the healthy proliferating cells and also in tumor cells to meet their elevated demand for guanine nucleotides. An exclusive regulatory mechanism for this enzyme is filamentation, through which IMPDH can resist allosteric inhibition by the end product, GTP. It has been proven that intracellular IMP, which rises during the proliferative state, potentially promotes IMPDH filamentation. On the other hand, interaction of IMPDH with ADP-ribosylation factor-like protein 13B (ARL13B) directs guanine biosynthesis toward the de novo pathway. However, ARL13B is not localized in the IMPDH-based cytoophidia, super structures composed of bundled IMPDH filaments and other proteins. Here, we hypothesized that ARL13B could increase availability of the de novo-produced IMP for IMPDH by interacting with the IMP-free IMPDH and microtubules adjacent to the purinosome. Following IMP-binding, IMPDH would be released from ARL13B and preferentially associated with its cytoophidia. Considering clinical side effects of catalytic inhibitors of IMPDH and their ability to induce IMPDH cytoophidia, we suggest that combination of proper doses of IMPDH catalytic inhibitors and inhibitors of the de novo IMP biosynthesis could be more effective in controlling cell proliferation.","PeriodicalId":483,"journal":{"name":"Biochemistry (Moscow)","volume":"89 12-13","pages":"2287 - 2291"},"PeriodicalIF":2.3,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142995391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of Non-Canonical Stacking Interactions of Heterocyclic RNA Bases in Ribosome Function 杂环RNA碱基非规范堆叠相互作用在核糖体功能中的作用

IF 2.3 4区生物学

Biochemistry (Moscow) Pub Date : 2025-01-17 DOI: 10.1134/S0006297924120137

Valeriy G. Metelev, Eugene F. Baulin, Alexey A. Bogdanov

引用次数: 0

Metabolomic Profiles of Siberian Wood Frog Rana amurensis in Hypoxia and Upon Reoxygenation 西伯利亚林蛙（Rana amurensis）在缺氧和复氧条件下的代谢组学特征

IF 2.3 4区生物学

Biochemistry (Moscow) Pub Date : 2025-01-17 DOI: 10.1134/S0006297924120034

Sergei V. Shekhovtsov, Nina A. Bulakhova, Yuri P. Tsentalovich, Nataliya A. Osik, Ekaterina N. Meshcheryakova, Tatiana V. Poluboyarova, Daniil I. Berman

{"title":"Metabolomic Profiles of Siberian Wood Frog Rana amurensis in Hypoxia and Upon Reoxygenation","authors":"Sergei V. Shekhovtsov, Nina A. Bulakhova, Yuri P. Tsentalovich, Nataliya A. Osik, Ekaterina N. Meshcheryakova, Tatiana V. Poluboyarova, Daniil I. Berman","doi":"10.1134/S0006297924120034","DOIUrl":"10.1134/S0006297924120034","url":null,"abstract":"Hypoxia poses a serious challenge for all animals; however, certain animals exhibit a remarkable resilience in the case of prolonged and severe hypoxia. The Siberian wood frog Rana amurensis is a unique amphibian capable of surviving for up to several months at almost complete anoxia. We investigated changes in the metabolome of R. amurensis at the onset of hypoxia (day 1) and within 1 h of reoxygenation after a long-term hypoxia using 1H NMR. We compared our results to the data obtained for animals exposed to 17 days of hypoxia and controls. Despite the differences between the samples analyzed in three different experimental series, we were able to obtain some interesting insights. In most studied vertebrates, succinate accumulates under hypoxic conditions and undergoes rapid conversion upon reoxygenation. We found that reoxygenation caused a decrease in the succinate content in the brain, but not in the liver, where it remained unchanged, suggesting an existence of a mechanism that inhibits succinate conversion. Furthermore, we observed intriguing differences in the behavior of two substances with unknown functions: glycerol and 2,3-butanediol. Glycerol exhibited rapid accumulation during hypoxia and equally rapid processing during reoxygenation. In contrast, 2,3-butanediol required an extended period of time to accumulate, yet persisted after reoxygenation. Overall, our data demonstrate rapid accumulation of most substances during exposure to hypoxia followed by their slower processing upon reoxygenation.","PeriodicalId":483,"journal":{"name":"Biochemistry (Moscow)","volume":"89 12-13","pages":"2133 - 2142"},"PeriodicalIF":2.3,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142995390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0