Tatiana Yu. Fufina, Lyudmila G. Vasilieva, Irina B. Klenina, Ivan I. Proskuryakov
{"title":"Anomalous Temperature Dependence of the Triplet-Triplet Energy Transfer in Cereibacter sphaeroides I(L177)H Mutant Reaction Centers","authors":"Tatiana Yu. Fufina, Lyudmila G. Vasilieva, Irina B. Klenina, Ivan I. Proskuryakov","doi":"10.1134/S0006297924090049","DOIUrl":"10.1134/S0006297924090049","url":null,"abstract":"<p>In photosynthetic reaction centers, quenching of the primary donor triplet state by energy transfer to the carotenoid molecule provides efficient suppression of generation of singlet-excited oxygen, potent chemical oxidant. This process in the<i> Cereibacter sphaeroides</i> reaction centers is thermoactivated, and discontinues at temperatures below 40 K. In these reaction centers, substitution of amino acid residue isoleucine at the 177 position of the L-subunit with histidine results in the sharp decrease of activation energy, so that the carotenoid triplets are populated even at 10 K. Activation energy of the <i>T-T</i> energy transfer was estimated as 7.5 cm<sup>–1</sup>, which is more than 10-fold lower than activation energy in the wild type reaction centers. At certain temperatures, the energy transfer in the mutant is decelerated, which is related to the increase of effective distance of the triplet-triplet transfer. To the best of our knowledge, the described mutation presents the first reaction center modification leading to the significant decrease in activation energy of the <i>T-T</i> energy transfer to carotenoid molecule. The I(L177)H mutant reaction centers present a considerable interest for further studies of the triplet state quenching mechanisms, and of other photophysical and photochemical processes in the reaction centers of bacterial photosynthesis.</p>","PeriodicalId":483,"journal":{"name":"Biochemistry (Moscow)","volume":"89 9","pages":"1573 - 1581"},"PeriodicalIF":2.3,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142411047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tatyana A. Druzhkova, Marina Yu. Zhanina, Elena E. Vladimirova, Alla B. Guekht, Natalia V. Gulyaeva
{"title":"Proteomic Spectrum of Serum Exosomes in Ischemic Stroke Patients Is Associated with Cognitive Impairment in the Post-Stroke Period","authors":"Tatyana A. Druzhkova, Marina Yu. Zhanina, Elena E. Vladimirova, Alla B. Guekht, Natalia V. Gulyaeva","doi":"10.1134/S0006297924090062","DOIUrl":"10.1134/S0006297924090062","url":null,"abstract":"<p>Ischemic stroke (IS) and subsequent neuropsychiatric disorders are among the leading causes of disability worldwide. Several strategies have been previously proposed to utilize exosomes for assessing the risk of IS-related diseases. The aim of this work was to evaluate serum exosomal proteins in IS patients during the chronic post-stroke period and to search for their associations with the development of post-stroke mild cognitive impairment (MCI). Comparative quantitative proteomic analysis of serum exosomes of patients without post-stroke MCI (19 patients mean age 52.0 ± 8.1 years) and patients with post-stroke MCI (11 patients, mean age 64.8 ± 5.6 years) revealed significant differences in the levels of 62 proteins out of 186 identified. Increased levels of the proteins associated with immune system and decreased levels of the proteins involved in lipid metabolism were observed in the patients with MCI compared to the patients without MCI in the chronic post-stroke period. The obtained data suggest that the higher level of immune system activation in the patients during a relatively long period after IS may be one of the risk factors for the development of post-stroke cognitive disorders and suggest participation of exosomal transport in these processes.</p>","PeriodicalId":483,"journal":{"name":"Biochemistry (Moscow)","volume":"89 9","pages":"1595 - 1609"},"PeriodicalIF":2.3,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142411054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ekaterina A. Guseva, Maria A. Emelianova, Vera N. Sidorova, Anatoly N. Tyulpakov, Olga A. Dontsova, Petr V. Sergiev
{"title":"Diversity of Molecular Functions of RNA-Binding Ubiquitin Ligases from the MKRN Protein Family","authors":"Ekaterina A. Guseva, Maria A. Emelianova, Vera N. Sidorova, Anatoly N. Tyulpakov, Olga A. Dontsova, Petr V. Sergiev","doi":"10.1134/S0006297924090037","DOIUrl":"10.1134/S0006297924090037","url":null,"abstract":"<p>Makorin RING finger protein family includes four members (MKRN1, MKRN2, MKRN3, and MKRN4) that belong to E3 ubiquitin ligases and play a key role in various biological processes, such as cell survival, cell differentiation, and innate and adaptive immunity. MKRN1 contributes to the tumor growth suppression, energy metabolism, anti-pathogen defense, and apoptosis and has a broad variety of targets, including hTERT, APC, FADD, p21, and various viral proteins. MKRN2 regulates cell proliferation, inflammatory response; its targets are p65, PKM2, STAT1, and other proteins. MKRN3 is a master regulator of puberty timing; it controls the levels of gonadotropin-releasing hormone in the arcuate nucleus neurons. MKRN4 is the least studied member of the MKRN protein family, however, it is known to contribute to the T cell activation by ubiquitination of serine/threonine kinase MAP4K3. Proteins of the MKRN family are associated with the development of numerous diseases, for example, systemic lupus erythematosus, central precocious puberty, Prader–Willi syndrome, degenerative lumbar spinal stenosis, inflammation, and cancer. In this review, we discuss the functional roles of all members of the MKRN protein family and their involvement in the development of diseases.</p>","PeriodicalId":483,"journal":{"name":"Biochemistry (Moscow)","volume":"89 9","pages":"1558 - 1572"},"PeriodicalIF":2.3,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142411053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Irina V. Pavlova, Nadezhda D. Broshevitskaya, Anastasiya A. Potekhina, Anastasiya M. Shvadchenko
{"title":"The Effect of Chronic Overcrowding on Social Behavior and Expression of Neuroinflammation-Associated Genes in Rats","authors":"Irina V. Pavlova, Nadezhda D. Broshevitskaya, Anastasiya A. Potekhina, Anastasiya M. Shvadchenko","doi":"10.1134/S0006297924090050","DOIUrl":"10.1134/S0006297924090050","url":null,"abstract":"<p>The effect of chronic overcrowding on the social behavior of adult male Wistar rats was studied. From postnatal day 30 (P30) to P180, the rats lived under standard (STND) or overcrowded (CRWD) conditions. Starting from P100, rat behavior was studied in the social preference and tube dominance tests, and aggressive behavior was investigated in the resident-intruder test. After decapitation of rats on P180, amygdala, dorsal hippocampus, ventromedial hypothalamus, and medial prefrontal cortex were collected and analyzed for expression of the IL-1β, TNF, TGF-β1, and IL-6 mRNAs by quantitative polymerase chain reaction. Compared to the STND group, rats from the CRWD group demonstrated shorter interaction time with a social object in the social preference test. They also had more wins in the tube test and initiated more attacks in the resident-intruder test. Expression of the <i>IL1β</i> gene in the hippocampus and medial prefrontal cortex and of the <i>TGFβ1</i> gene in the hippocampus, amygdala, and prefrontal cortex was increased in the CRWD group. The stress induced by overcrowding increased social dominance and aggressiveness and decreased social motivation in rats. The changes in the social behavior of CRWD rats were accompanied by upregulation of expression of genes for the proinflammatory cytokine IL-1β and the anti-inflammatory cytokine TGF-β1 in a number of brain structures, which can be considered as manifestations of neuroinflammation and compensatory processes, respectively.</p>","PeriodicalId":483,"journal":{"name":"Biochemistry (Moscow)","volume":"89 9","pages":"1582 - 1594"},"PeriodicalIF":2.3,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142410809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Maria A. Zamotina, Lidia K. Muranova, Artur I. Zabolotskii, Pyotr A. Tyurin-Kuzmin, Konstantin Yu. Kulebyakin, Nikolai B. Gusev
{"title":"Universal Adapter Protein Bag3 and Small Heat Shock Proteins","authors":"Maria A. Zamotina, Lidia K. Muranova, Artur I. Zabolotskii, Pyotr A. Tyurin-Kuzmin, Konstantin Yu. Kulebyakin, Nikolai B. Gusev","doi":"10.1134/S0006297924090013","DOIUrl":"10.1134/S0006297924090013","url":null,"abstract":"<p>Bag3 (Bcl-2-associated athanogene 3) protein contains a number of functional domains and interacts with a wide range of different partner proteins, including small heat shock proteins (sHsps) and heat shock protein Hsp70. The ternary Bag3–sHsp–and Hsp70 complex binds denatured proteins and transports them to phagosomes, thus playing a key role in the chaperone-assisted selective autophagy (CASA). This complex also participates in the control of formation and disassembly of stress granules (granulostasis) and cytoskeleton regulation. As Bag3 and sHsps participate in multiple cellular processes, mutations in these proteins are often associated with neurodegenerative diseases and cardiomyopathy. The review discusses the role of sHsps in different processes regulated by Bag3.</p>","PeriodicalId":483,"journal":{"name":"Biochemistry (Moscow)","volume":"89 9","pages":"1535 - 1545"},"PeriodicalIF":2.3,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1134/S0006297924090013.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142410976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Daria V. Goliusova, Margarita Y. Sharikova, Kristina A. Lavrenteva, Olga S. Lebedeva, Lidia K. Muranova, Nikolai B. Gusev, Alexandra N. Bogomazova, Maria A. Lagarkova
{"title":"Role of Filamin C in Muscle Cells","authors":"Daria V. Goliusova, Margarita Y. Sharikova, Kristina A. Lavrenteva, Olga S. Lebedeva, Lidia K. Muranova, Nikolai B. Gusev, Alexandra N. Bogomazova, Maria A. Lagarkova","doi":"10.1134/S0006297924090025","DOIUrl":"10.1134/S0006297924090025","url":null,"abstract":"<p>Filamin C (FLNC) is a member of a high-molecular weight protein family, which bind actin filaments in the cytoskeleton of various cells. In human genome FLNC is encoded by the <i>FLNC</i> gene located on chromosome 7 and is expressed predominantly in striated skeletal and cardiac muscle cells. Filamin C is involved in organization and stabilization of thin actin filaments three-dimensional network in sarcomeres, and is supposed to play a role of mechanosensor transferring mechanical signals to different protein targets. Under mechanical stress FLNC can undergo unfolding that increases the risk of its aggregation. FLNC molecules with an impaired native structure could be eliminated by the BAG3-mediated chaperone-assisted selective autophagy. Mutations in the <i>FLNC</i> gene could be accompanied by the changes in FLNC interaction with its protein partners and could lead to formation of aggregates, which overload the autophagy and proteasome protein degradation systems, thus facilitating development of various pathological processes. Molecular mechanisms of the <i>FLNC</i>-associated congenital disorders, called filaminopathies, remain poorly understood. This review is devoted to analysis of the structure and mechanisms of filamin C function in muscle and heart cells in normal state and in the <i>FLNC</i>-associated pathologies. The presented data summarize the results of research at the molecular, cellular, and tissue levels and allow us to outline promising ways for further investigation of pathogenetic mechanisms in filaminopathies.</p>","PeriodicalId":483,"journal":{"name":"Biochemistry (Moscow)","volume":"89 9","pages":"1546 - 1557"},"PeriodicalIF":2.3,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142410808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ivan B. Filippenkov, Nataliya Y. Glazova, Elena A. Sebentsova, Vasily V. Stavchansky, Lyudmila A. Andreeva, Nikolai F. Myasoedov, Nataliya G. Levitskaya, Svetlana A. Limborska, Lyudmila V. Dergunova
{"title":"Changes of Transcriptomic Activity in Rat Brain Cells under the Influence of Synthetic Adrenocorticotropic Hormone-Like Peptides","authors":"Ivan B. Filippenkov, Nataliya Y. Glazova, Elena A. Sebentsova, Vasily V. Stavchansky, Lyudmila A. Andreeva, Nikolai F. Myasoedov, Nataliya G. Levitskaya, Svetlana A. Limborska, Lyudmila V. Dergunova","doi":"10.1134/S0006297924090104","DOIUrl":"10.1134/S0006297924090104","url":null,"abstract":"<p>Synthetic peptides have a wide range of clinical effects. Of particular interest are peptides based on adrenocorticotropic hormone (ACTH) both as already used and as potential drugs for preventing consequences of cerebral ischemia. However, it is necessary to study influence of the peptide on the brain cells under normal physiological conditions, including understanding the risks of their use. Here, we used high-throughput RNA sequencing (RNA-Seq) to identify differentially expressed genes (DEGs) in the brain frontal cortex of rat receiving intraperitoneal administration of ACTH-like peptides ACTH(4-7)PGP (Semax) and ACTH(6-9)PGP, or saline. We identified 258 and 228 DEGs, respectively, with the fold change > 1.5 and <i>Padj</i> < 0.05 at 22.5 h after the first administration of Semax and ACTH(6-9)PGP. Metabolic pathways, characterizing both common and specific effects of the peptides on the transcriptome were identified. Both peptides predominantly caused decrease in expression of the genes associated with the immune system. At the same time, when comparing the effects of ACTH(6-9)PGP relative to Semax, DEGs were identified that characterized the main differences in the effects of the peptides. These genes were mostly downregulated and associated with neurosignaling systems and regulation of ion channels, thus characterizing differences in the effects of the peptides. Our data show how differences in the structure of ACTH derivatives are associated with the changes in the brain cell transcriptome following exposure to these related peptides. Furthermore, our results demonstrate that when studying influence of regulatory peptides on transcriptome under pathological conditions, it is necessary to take into account their actions under normal physiological conditions.</p>","PeriodicalId":483,"journal":{"name":"Biochemistry (Moscow)","volume":"89 9","pages":"1643 - 1656"},"PeriodicalIF":2.3,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142411055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Efficient Production and Purification of Bioactive E50-52-Class IIa Peptidic Bacteriocin Is Achieved through Fusion with the Catalytic Domain of Lysostaphin-Class III Bacteriocin","authors":"Nichakarn Phrutpoom, Tararat Khaokhiew, Aung Khine Linn, Somsri Sakdee, Chompounoot Imtong, Nujarin Jongruja, Chanan Angsuthanasombat","doi":"10.1134/S0006297924090074","DOIUrl":"10.1134/S0006297924090074","url":null,"abstract":"<p>E50-52, a class IIa-peptidic bacteriocin produced by a strain of <i>Enterococcus faecium</i>, has broad-spectrum antimicrobial activity against various foodborne pathogens. However, effective utilization of the E50-52 has been limited by low production yields and challenges associated with separation and purification of this 39-amino acid antimicrobial peptide. In this study, we have successfully produced a biologically active recombinant form of E50-52 by fusing it with the 16-kDa catalytic domain of lysostaphin-class III bacteriocin (LssCAT), which resulted in high-yield production. Initially, the LssCAT-E50-52 chimeric protein was insoluble upon over-expression in <i>Escherichia coli</i>, but it became soluble using phosphate buffer (pH 7.4) supplemented with 8 M urea. Purification using immobilized-Ni<sup>2+</sup> affinity chromatography under urea denaturing conditions resulted in consistent production a homogenous products (LssCAT-E50-52) with >95% purity. The purified protein was refolded using an optimized stepwise dialysis process. The resulting refolded LssCAT-E50-52 protein exhibited dose-dependent inhibitory activity against <i>Helicobacter pylori</i>, a Gram-negative, flagellated, helical bacterium that is associated with gastric cancer. Overall, the optimized protocol described in this study effectively produced large quantities of high-purity recombinant LssCAT-E50-52 protein, yielding approximately 100 mg per liter of culture. To the best of our knowledge, this is the first report on the impact of LssCAT-E50-52 on <i>H. pylori</i>. This finding could pave the way for further research into bactericidal mechanism and potential applications of this bacteriocin in biomedical industry.</p>","PeriodicalId":483,"journal":{"name":"Biochemistry (Moscow)","volume":"89 9","pages":"1610 - 1618"},"PeriodicalIF":2.3,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142410810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yana V. Serdyuk, Ksenia V. Zornikova, Dmitry V. Dianov, Nataliia O. Ivanova, Vassa D. Davydova, Ekaterina I. Fefelova, Tatiana A. Nenasheva, Saveliy A. Sheetikov, Apollinariya V. Bogolyubova
{"title":"T-Cell Receptors Cross-Reactive to Coronaviral Epitopes Homologous to the SPR Peptide","authors":"Yana V. Serdyuk, Ksenia V. Zornikova, Dmitry V. Dianov, Nataliia O. Ivanova, Vassa D. Davydova, Ekaterina I. Fefelova, Tatiana A. Nenasheva, Saveliy A. Sheetikov, Apollinariya V. Bogolyubova","doi":"10.1134/S0006297924090098","DOIUrl":"10.1134/S0006297924090098","url":null,"abstract":"<p>The COVID-19 pandemic caused by the rapid spread of the novel coronavirus SARS-CoV-2, has promoted an interest in studying the T-cell immune response. It was found that the polyclonal and cross-reactive T-cell response against seasonal coronaviruses and other SARS-CoV-2 strains reduced disease severity. We investigated the immunodominant T-cell epitope SPRWYFYYYL from the nucleocapsid protein of SARS-CoV-2. The immune response to this epitope is characterized by the formation of highly homologous (convergent) receptors that have been found in the T-cell receptor (TCR) repertoires of different individuals. This epitope belongs to a group of highly conserved peptides that are rarely mutated in novel SARS-CoV-2 strains and are homologous to the epitopes of seasonal coronaviruses. It has been suggested that the cross-reactive response to homologous peptides contributes to the reduction of COVID-19 severity. However, some investigators have questioned this hypothesis, suggesting that the low affinity of the cross-reactive receptors reduces the strength of the immune response. The aim of this study was to evaluate the effect of amino acid substitutions in the SPR epitope on its binding affinity to specific TCRs. For this, we performed antigen-dependent cellular expansions were performed using samples from four COVID-19-transfected donors and sequenced their TCR repertoires. The resulting SPR-specific repertoire of β-chains in TCRs had a greater sequence diversity than the repertoire of α-chains. However, the TCR repertoires of all four donors contained public receptors, three of which were cloned and used to generate the Jurkat E6-1 TPR cell line. Only one of these receptors was activated by the SPR peptide and recognized with the same affinity by its mutant homologue LPRWYFYYY from seasonal coronaviruses. This indicates that the presence of the mutation did not affect the strength of the immune response, which may explain why the cross-reactive response to the SPR epitope is so frequent and contributes positively to COVID-19 infection.</p>","PeriodicalId":483,"journal":{"name":"Biochemistry (Moscow)","volume":"89 9","pages":"1631 - 1642"},"PeriodicalIF":2.3,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142411049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yifen Shen, Yanping Hu, Tao Yang, Hao Shen, Genhai Shen, Yuriy L Orlov, Shasha Zhou, Yihang Shen
{"title":"Development of Serum Cell-Free miRNA Panel for Identification of Central Precocious Puberty and Premature Thelarche in Girls.","authors":"Yifen Shen, Yanping Hu, Tao Yang, Hao Shen, Genhai Shen, Yuriy L Orlov, Shasha Zhou, Yihang Shen","doi":"10.1134/S0006297924100134","DOIUrl":"https://doi.org/10.1134/S0006297924100134","url":null,"abstract":"<p><p>Precocious puberty of children, especially girls, has attracted more and more public attention in recent years. In clinic practice, there is a lack of both convenient and effective way to identify central precocious puberty (CPP) and premature thelarche (PT). In this study, we enrolled total 88 girls [28 cases of CPP, 37 cases of PT, as well as 23 cases of normal control (NC)] as a training cohort, and another 270 subjects (92 cases of CPP, 122 cases of PT and 56 cases of NC) as a validation cohort. Expression of serum cell-free miRNA in the training cohort was analyzed using five different methods to identify specific miRNA feature subsets, and verified by qPCR in the validation cohort. Here, we determined that the combination of miRNAs (miR-584-5p, miR-625-3p, miRNA-652-3p, miR-22-3p) provided the possibility to distinguish CPP and PT. The miRNA panel (miR-625-3p, let-7b-5p, miR-140-5p, miR-7-5p) had the best performance in distinguishing between CPP and NC. The miRNA panel (miR-140-5p, miR-205-5p, let-7b-5p, miR-629-5p, miR-9-3p) performed well in identifying PT and NC. Based on the absolute quantification of miRNA by qPCR, we also presented three regression equations to evaluate CPP, PT, and NC, respectively, for possible use in clinical practice. The presented study had identified several sets of miRNA panels as biomarkers to assist in identifying CPP and PT. Our invention could provide better diagnostic tool for pediatric precocious puberty diseases in both clinical and public health fields.</p>","PeriodicalId":483,"journal":{"name":"Biochemistry (Moscow)","volume":"89 10","pages":"1834-1843"},"PeriodicalIF":2.3,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142611922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}