Faktori eksperimentalʹnoï evolûcìï organìzmìv最新文献

{"title":"Analysis of KIR genotyping results in women with recurrent implantation failure","authors":"K. O. Sosnina, D. V. Zastavna, O. I. Terpyliak","doi":"10.7124/feeo.v33.1572","DOIUrl":"https://doi.org/10.7124/feeo.v33.1572","url":null,"abstract":"Aim. To analyze the frequency and distribution of KIR genotypes in women with recurrent implantation failure after cycles of in vitro fertilization. Methods. DNA extraction and purification, PCR-SSP, agarose gel electrophoresis. Results. The spectrum of KIR genes and the frequency of KIR genotypes in women with recurrent implantation failure were determined. The study group was divided according to history, in particular, a group of 84 women whose diagnosis was outlined by the concept of \"repeated failed implantations\" was singled out. Statistical analysis of the obtained results using the Pearson test (χ2) indicates a significantly lower frequency of the KIR-AB genotype (χ2 = 9.7; p < 0.005) and a significantly higher frequency of the KIR-AA genotype (χ2 = 7.35; p < 0.01) in women with repeated failed implantations compared to the general group of women with reproductive failures. Conclusions. Taking into account the results of statistical data processing (OR = 2.05; CI 95 %: 1.21-3.45), we consider the KIR-AA genotype to be a significant risk factor for recurrent implantation failure after IVF. We define KIR genotyping as a genetic test to assess the risk of the embryo being rejected by the maternal immune system, and thus to direct medical interventions in order to achieve a successful pregnancy.","PeriodicalId":475640,"journal":{"name":"Faktori eksperimentalʹnoï evolûcìï organìzmìv","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135428631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plant genome modification technologies and some aspects of their practical use","authors":"L. O. Mykhalskyi","doi":"10.7124/feeo.v32.1555","DOIUrl":"https://doi.org/10.7124/feeo.v32.1555","url":null,"abstract":"Aim. To analyze modern methods of plant genome modification on the example of cereals and to evaluate the prospects of their use and practical significance. Methods. A review of available scientific sources and electronic resources containing data on existing and promising methods of genetic modification of cereals was conducted. Results. During the entire time of its development, mankind solved the issue of creating more productive agricultural crops that were adapted to the climatic conditions of existence. For this, domestication and selection based on spontaneous mutations were traditionally used. New technologies of genetic recombination, which have been developed in recent decades, make it possible to change the genome of plants in certain sites determined by the researcher. This has opened up unprecedented opportunities both in fundamental terms and in a whole range of applied aspects for elucidating the functions of specific genes and targeted improvement of the productivity of plants and some of them properties. Conclusions. Some modern new technologies of plant genome modifications are of significant scientific interest and practical importance. The use of these technologies is extremely important in many practical areas, especially for wheat and barley as the most agronomically important cereal crops in the world. Genome modifications agricultural crops require significant attention in terms of their safety and legal regulation of practical use. Information about modern methods of plant genome modification and directions for their use should be a mandatory element in educational courses on modern biotechnology in specialized educational institutions.","PeriodicalId":475640,"journal":{"name":"Faktori eksperimentalʹnoï evolûcìï organìzmìv","volume":"59 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135427650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Walther Flemming: life path and scientific legacy","authors":"O. Yu. Mayorova, M. Z. Prokopiak, N. Y. Mishchuk, O. M. Zagrychuk, L. R. Hrytsak, N. M. Drobyk","doi":"10.7124/feeo.v33.1591","DOIUrl":"https://doi.org/10.7124/feeo.v33.1591","url":null,"abstract":"The main stages of Walther Flemming life and activity are considered in the article. Walther Flemming (21.04.1843–4.08.1905) is German biologist, anatomist, one of the most famous histologists of the 19th century and the founder of cytogenetics. He studied medicine at four famous universities of the German Empire – in Göttingen, Tübingen, Berlin and Rostock. After defending his thesis, in 1868 he started teaching anatomy and histology at Würzburg, Amsterdam and Rostock universities, Germany and Charles Ferdinand University, the Czech Republic. During the last 25 years of his life, he was a professor of anatomy at the Christian Albrecht University in Kiel, Germany and headed one of its departments – the anatomical institute.&#x0D; At the beginning of W. Flemming scientific activity, his research mostly concerned histology and anatomy, as well as the development of histological methods and fixation of microscopic specimens. However, the main works of the scientist are devoted to study cell division process and the chromosomes distribution in daughter nuclei. He firstly introduced the concept of “mitosis”, which he studied on stained microscopic preparations, using salamander epithelial cells as a source of biological material. W. Flemming described the processes in the nucleus, distinguished “progressive” and “regressive” phases of cell division. He demonstrated that the cells contain areas that absorb basophilic dyes. These areas were later called chromatin; other structures remained unstained under such conditions and were therefore called achromatin. W. Flemming studied the structure and functions of the spindle during cell division, and he also studied the polar structure, which he called centriole. The scientist found that indirect division usually occurs not only in vertebrates, but also in invertebrates and protists. He established that two successive, morphologically distinct divisions of nuclear occur during spermatogenesis, resulting in the division of chromatin into two daughter cells. Thus, W. Flemming’s research contributed to the development of cell biology and its branches.","PeriodicalId":475640,"journal":{"name":"Faktori eksperimentalʹnoï evolûcìï organìzmìv","volume":"40 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135427660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Calli cultures obtained via selection with heavy metal ions and their osmotic tolerance","authors":"I. A. Zaitseva, L. I. Bronnikova","doi":"10.7124/feeo.v32.1540","DOIUrl":"https://doi.org/10.7124/feeo.v32.1540","url":null,"abstract":"Aim. It is established that the resistance to some heavy metal cations is combined with the osmotic stress tolerance. The resistance to barium ions correlates with the salinity tolerance whereas the cadmium cation resistance is combined with water stress deficit. It is known too about the differences between the tolerance levels of cell cultures and tolerance levels of plants. On this account the comparative estimation of the primary and secondary calli to simulated stresses is established. Methods. The tobacco was the object of the experiment. This plant is extremely sensitive to osmotic stresses. The salinity was simulated by the addition of sea water salts – 25.0 g/l; water stress was created by the addition of 0.8M of mannitol As calli proliferation marker relative fresh mass growth was used. Results. Primary and secondary tobacco calli there were obtained. Those cultures demonstrated resistance to lethal simulated stresses. Ва-resistant culture developed on medium with the addition of 25.0 g/l of sea water salts. Cd-resistant culture grew on medium. Conclusions. The appearance of tolerance is selected after the primary selection on media with heavy metal ions. The level of the tolerance did not decrease within the cultivating period.","PeriodicalId":475640,"journal":{"name":"Faktori eksperimentalʹnoï evolûcìï organìzmìv","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135428283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Androgenic response of spring barley (Hordeum vulgare L.) varieties and lines – sources of high grain protein content","authors":"O. V. Bilynska, T. А. Shelyakina","doi":"10.7124/feeo.v32.1538","DOIUrl":"https://doi.org/10.7124/feeo.v32.1538","url":null,"abstract":"Aim. Protein content is known to be one of the most important characters of grain quality. Investigation was aimed to determine the capability to haploid production in anther culture in vitro of spring barley accessions which are the sources of high protein content in grain. Methods. Infrared spectroscopy was used for evaluation of variability of barley genotypes in protein content. Anthers isolated from cold-pretreated spikes were cultivated on agar solidified nutrient medium containing N6 macro-and MS micronutrients, physiologically active substances and maltose (9.0 %). Results. Three years testing showed that mean grain protein contents in six spring barley accessions varied from 12.6 to 19.1 %. Commercial variety Yavir, accessions IR06605 and DD-21 from germplasm collection of the National Plant Gene Pool of Ukraine were selected as genotypes with a stable expression of this char-acter. Response to androgenesis in vitro varied from 6.9 to 45.9 % of morphogenic anthers and from 0.64 до 14.7 green plants per 100 cultivated anthers in dependence of genotype and donor plant growth conditions. Conclusions. Because of association between high protein content in grains and a relatively high androgenic response, varieties Velikan and Yavir were considered to be the most valuable ones for use in spring barley breeding for improvement of protein content via anther culture in vitro.","PeriodicalId":475640,"journal":{"name":"Faktori eksperimentalʹnoï evolûcìï organìzmìv","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135428297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differences in amino acid composition of carrot α-tubulin potentially confer the resistance to dinitroaniline herbicides","authors":"O. G. Melnyk, R. Y. Blume, P. A. Karpov","doi":"10.7124/feeo.v32.1534","DOIUrl":"https://doi.org/10.7124/feeo.v32.1534","url":null,"abstract":"Aim. To reveal the features of amino acid composition of carrot α-tubulin isotypes that potentially determine natural tolerance to dinitroaniline herbicides. Methods. Literature and database search. Comparison of protein sequences and structures: multiple sequence alignment, phylogenetic profiling, protein and ligand structure modeling, etc. Results. Genomic and proteomic analysis of Daucus carota has revealed at least eight unique isotypes of α-tubulin that differ in amino acid sequences and gene loci. Remarkable differences in amino acid composition of the dinitroanilinebinding-like (DBL) region of analyzed α-tubulin have been revealed, which may be the reason of its natural resistance to these compounds. Сonclusions. Differences in amino acids at positions of canonical mutations – Cys4 (TBA1, 2, 3, 6, 7 and 8), Thr53 (TBA6), Ile202 (TBA1 and 7) and Met202 (TBA5), as well as previously undescribed non-canonical substitutions – Ile4 (TBA4 and 5), Cys52 (TBA6), Ser201 (TBA1, 2, 3 and 8) and Val194 (TBA4 and 5), were noted as potentially associated with natural tolerance of the carrot to dinitroaniline herbicides.","PeriodicalId":475640,"journal":{"name":"Faktori eksperimentalʹnoï evolûcìï organìzmìv","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135427358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distribution of the macaques genus-specific Alu repeat AluMacYa3 in the MGMT gene orthologs of old world monkeys","authors":"O. V. Pidpala, L. L. Lukash","doi":"10.7124/feeo.v32.1552","DOIUrl":"https://doi.org/10.7124/feeo.v32.1552","url":null,"abstract":"Aim. To follow the distribution and evolution of the macaques genus-specific Alu repeat AluMacYa3 in Cercopithecidae MGMT gene orthologs. Methods. The homology between nucleotide sequences has been determined by the BLAST 2.6.1 program. The results of the search and identification of MGE have been obtained by the CENSOR program. Results. It has been shown on the example of Cercopithecidae MGMT gene orthologs that the genus-specific Alu repeat AluMacYa3 is present not only in the intron sequences of macaques, but also in other representatives of the Old World monkeys and in its evolutionary history not a deletion degradation of nucleotide sequences, as it is in the case of species-specific Alu repeats, but a single nucleotide polymorphism is dominated. Conclusions. Thus, the macaque genus-specific Alu repeat AluMacYa3 has been identified in different representatives of the Old World monkeys and its evolutionary history combines nucleotide polymorphism and deletion degradation of nucleotide sequences.","PeriodicalId":475640,"journal":{"name":"Faktori eksperimentalʹnoï evolûcìï organìzmìv","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135427507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the phenotypic polymorphism of the pattern of Leptinotarsa decemlineata Say pronotum in the conditions of Kamianka-Buzka city (Lviv Oblast)","authors":"M. A. Kryzhanovska, M. Z. Prokopiak, H. M. Holinei, L. O. Shevchyk","doi":"10.7124/feeo.v33.1589","DOIUrl":"https://doi.org/10.7124/feeo.v33.1589","url":null,"abstract":"Aim. The Colorado potato beetle population is characterized by a high degree of polymorphism. This provides a high level of adaptability to the most diverse distribution conditions. The aim is to the study of the variability of the pattern of Leptinotarsa decemlineata pronotum in June 2021 and June 2022. Methods. The analysis of the phenotypic variability of L. decemlineata population of Kamianka-Buzka city based on studying the presence or absence of certain phens, their number and placement according to Tauer method. Results. 22 variations of phens belonging to A, B, C, D, E, G, K, L, M, P groups were found. The dominant were phens of A, B, C, D, E, F groups. The most variable were the phens of A and E groups. The phenotypic structure of the colorado beetle population was determined according to Kohmaniuk’s classification. It was found that the dominant morphs in 2021 and 2022 were U (42 % and 27 %), UP (13 % and 18 %) and V (23 % and 21 %), respectively. Conclusions. The analysis of the frequency of phenoforms according to the Fasulati method showed that in the population of 2021 and 2022, phenoforms 3, 6 and 9 were dominant. They form the basis of the population and ensure the resistance of the population to the insecticides. The study of the intrapopulation diversity indicates an unbalanced population structure of L. decemlineata.","PeriodicalId":475640,"journal":{"name":"Faktori eksperimentalʹnoï evolûcìï organìzmìv","volume":"2012 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135427664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green infrastructure, urbanization and ecosystem services – relationships in a megalopolis","authors":"N. V. Miroshnyk","doi":"10.7124/feeo.v33.1581","DOIUrl":"https://doi.org/10.7124/feeo.v33.1581","url":null,"abstract":"Aim. Analyzed the relationship between urbanization and ecosystem services of green spaces in the metropolis, identified risks and promising ways to improve management. Methods. Methods of comparative analysis, classification, ranking of impacts were used. Results. The relationship between urbanization and ecosystem services of green spaces has been studied on the example of the urban ecosystem of Kyiv. The risks of fragmentation, destruction, adventization, underground infrastructure, hydrological regime, and unsuccessful management was analyzed. The characteristics of urbanization and climate change for the urban ecosystem of Kyiv was presented. Conclusions. In the long term, improving the quality of green infrastructure ecosystem services can be achieved by reducing artificial surfaces, increasing forest areas and parks within the city, possibly through new technologies for vertical gardening, green roofs, rooftop parks, and economic incentives for the conservation of multi-tiered plantings of a large area. It is necessary to create continuous ecological networks of green spaces within the state, unite fragmented biotopes and preserve unique biodiversity, maintain regional environmental security.","PeriodicalId":475640,"journal":{"name":"Faktori eksperimentalʹnoï evolûcìï organìzmìv","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135427901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Realization of productive potential in winter wheat under drought","authors":"O. I. Zhuk, O. O. Stasik","doi":"10.7124/feeo.v33.1560","DOIUrl":"https://doi.org/10.7124/feeo.v33.1560","url":null,"abstract":"Aim. The aim of the work was to study the realization of the productive potential of bread winter wheat plants under drought (Triticum aestivum L.). Methods. Wheat plants cultivars Bogdana and Perlina Podyllja were grown under optimal conditions until the earing-flowering phase, after that the experimental plants were transferred to drought regime for 8 days. Optimal water supply was restored to the end of vegetation. Leaf surface area, mass of shoot and grains were measured during the experiment. Ripened plants were analyzed by the yield structure. Results. It was established that the effect of water deficit in the critical phase of ontogenesis of earing-flowering caused decreasing of leaf surface area, mass of shoots and grains more significantly in the Perlyna Podillia cultivar compared to the Bogdana cultivar. Restoration of irrigation stimulated the growth of shoots and grains, but did not compensate for the loss of their number. Conclusions. Water deficit in soil in critical earing-flowering phase delayed the increasing mass of shoots, grains and the area of leaves that caused the decrease in plant productivity.","PeriodicalId":475640,"journal":{"name":"Faktori eksperimentalʹnoï evolûcìï organìzmìv","volume":"60 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135428289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}