Faktori eksperimentalʹnoï evolûcìï organìzmìv最新文献_第4页

Structural profile of ligand-based inhibition of bacterial FtsZ 配体抑制细菌FtsZ的结构特征

Faktori eksperimentalʹnoï evolûcìï organìzmìv Pub Date : 2023-09-01 DOI: 10.7124/feeo.v32.1551

D. S. Ozheriedov, P. A. Karpov

{"title":"Structural profile of ligand-based inhibition of bacterial FtsZ","authors":"D. S. Ozheriedov, P. A. Karpov","doi":"10.7124/feeo.v32.1551","DOIUrl":"https://doi.org/10.7124/feeo.v32.1551","url":null,"abstract":"Aim. The idea of the study was to compare and generalize RCSB Protein Data Bank and ChEMBL data in order to establish the structural and biological relationship of experimentaly proved effectors of FtsZ with binding sites. Methods. Literature and database search. Comparison of protein and ligand structures. Protein structure modeling, MD, structural superimposition, etc. Results. The experimental protein-ligand complexes structures of bacterial FtsZ were revised. The structural superimposition of experinental PDB and full-atomic AlphaFold2 models of bacterial FtsZs confirmed their significant structural similarity. Three protein-ligand binding sites were identified by structural alignment. The rating based on database (RCSB Protein Data Bank, ChEMBL, DrugBank, BindingDB, PubChem), patente and literature information on FtsZ-ligand interactions identify perspective sites and main reference compounds. Сonclusions. It was identifyd 3 main protein-ligand binding regions in FtsZ: I. Nucleotide Binding Domain (Ia. Site of GTP/GDP and Ib. MB3 site); II. Site of inter-domain cleft (IDC) and III. Site of coumarin bindig (4HC = 4-hydroxycoumarin). It was indicated that benzamide-binding site, located in the region of inter-domain cleft of FtsZ, demonstrate highest site- and target-specificity.","PeriodicalId":475640,"journal":{"name":"Faktori eksperimentalʹnoï evolûcìï organìzmìv","volume":"78 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135427506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cytogenetic changes in irradiated rats under influence of guaninium-zinc chloride complex 鸟-氯化锌复合物对辐照大鼠细胞遗传学的影响

Faktori eksperimentalʹnoï evolûcìï organìzmìv Pub Date : 2023-09-01 DOI: 10.7124/feeo.v33.1590

E. N. Shamilov, A. S. Abdullayev, V. E. Shamilli, M. F. Farajov, I. V. Azizov, K. A. Hajiyev, A. A. Jalilova

{"title":"Cytogenetic changes in irradiated rats under influence of guaninium-zinc chloride complex","authors":"E. N. Shamilov, A. S. Abdullayev, V. E. Shamilli, M. F. Farajov, I. V. Azizov, K. A. Hajiyev, A. A. Jalilova","doi":"10.7124/feeo.v33.1590","DOIUrl":"https://doi.org/10.7124/feeo.v33.1590","url":null,"abstract":"Aim. The main goal of this study was to study the anti-radiation effect of guaninium-zinc chloride complex on cytogenetic changes in irradiated rats. Methods. White rats of the Wistar line with an average weight of 110-125 grams served as the material for the detection of the anti-radiation effect of guaninium-zinc chloride complex. Animals were injected intraperitoneally with solutions of guaninium-zinc chloride complex at a concentration of 40 mg/kg 2 hours before irradiation and were subjected to general uniform gamma irradiation from a 60Co source in the Rukhund-20000 irradiation unit at a dose of 3 and 5 Gy. Results. Cytogenetic analysis of animals showed that with a single injection of guaninium-zinc chloride complex solutions before irradiation, the proportion of chromosomal abnormalities decreased markedly, and reached 2.93 %, and the amount of abnormal spermatozoa markedly decreased. Guaninium-zinc chloride complex softened the damaging effects of ionizing radiation. There was no suppression of cell division. Conclusions. It was found for the first time that, with the introduction of a solution of guaninium-zinc chloride complex in white Wistar rats before irradiation, it reduces the damaging effect of gamma irradiation. The obtained data gives grounds to assume that the guaninium-zinc chloride complex tested by us can be recommended as a promising anti-radiation agent.","PeriodicalId":475640,"journal":{"name":"Faktori eksperimentalʹnoï evolûcìï organìzmìv","volume":"103 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135427665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of dextran-70 on the cytotoxic effect of EMAP II on glioma cells in vitro 葡聚糖-70对EMAP II对胶质瘤细胞毒作用的影响

Faktori eksperimentalʹnoï evolûcìï organìzmìv Pub Date : 2023-09-01 DOI: 10.7124/feeo.v32.1549

I. M. Shuba, V. V. Lylo, I. S. Karpova, O. Y. Glavatskyi, O. I. Kornelyuk

{"title":"Influence of dextran-70 on the cytotoxic effect of EMAP II on glioma cells in vitro","authors":"I. M. Shuba, V. V. Lylo, I. S. Karpova, O. Y. Glavatskyi, O. I. Kornelyuk","doi":"10.7124/feeo.v32.1549","DOIUrl":"https://doi.org/10.7124/feeo.v32.1549","url":null,"abstract":"Aim. To evaluate with the use of the MTT test how the formation of a nanocomposite complex of the cytokine EMAP II with dextran-70 affects the cytotoxic effect of the free protein in glioma cell cultures of different origins: a standart culture U251MG cells and a primary culture of cells obtained from tumor fragments. Methods. The recombinant polypeptide EMAP II was obtained using gene engineering biotechnology. The study was conducted on the standard human glioma cell line U251MG and on primary cell cultures obtained from tumor fragments after surgical removal. Cell viability was determined using the MTT test. The cells were cultivated for a day with t the different concentrations from 1.0 pM to 10 μM of EMAP II and the EMAP II + dextran-70 complex im serum-free standard DMEM growth medium. Results. In our previous works, it has been shown that EMAP II exhibited dose-dependent cytotoxic properties in the concentration range of 1.0 pM – 10 μM on U251MG glioma cells and in primary cell cultures. The dose-effect curve in all cases has a complex pattern. Dextran-70 does not fundamentally affect the cytotoxic effect of EMAP II, but depending on the dose and type of cells, it shows the ability to slightly weaken/enhance the effect of the free protein. An increased sensitivity of the primary culture of glioma cells to the cytotoxic effect of the EMAP II + dextran-70 complex was revealed. Conclusions. Dextran-70 does not fundamentally affect the cytotoxic effect of EMAP II, but depending on the dose and type of cells, it shows the ability to slightly weaken/enhance the effect of the latter.","PeriodicalId":475640,"journal":{"name":"Faktori eksperimentalʹnoï evolûcìï organìzmìv","volume":"58 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135427880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sensitivity of Taraxacum officinalis (L.) Weber ex F. H. Wigg Pollen to aerogenic pollution in the conditions of a mega city 蒲公英的敏感性韦伯和F. H. Wigg Pollen对大城市条件下的空气污染进行了研究

Faktori eksperimentalʹnoï evolûcìï organìzmìv Pub Date : 2023-09-01 DOI: 10.7124/feeo.v33.1564

M. Yu. Mazura

{"title":"Sensitivity of Taraxacum officinalis (L.) Weber ex F. H. Wigg Pollen to aerogenic pollution in the conditions of a mega city","authors":"M. Yu. Mazura","doi":"10.7124/feeo.v33.1564","DOIUrl":"https://doi.org/10.7124/feeo.v33.1564","url":null,"abstract":"Aim. To determine the consequences of the impact of automobile emissions on the environment of the city of Kyiv using the palynological method. Methods. The quality of Taraxacum officinalis (L.) Weber ex F. H. Wigg pollen was determined by the iodine method (Gram staining). All pollen grains without typical signs of morphological structure (that is, all but normally developed) were considered teratomorphic. Results. The effect of aerogenous load on the viability of Taraxacum officinalis plant pollen was studied at test sites of park ecosystems of the city of Kyiv with different traffic intensities and proximity to city highways. It was established that in the conditions of the metropolis under various effects of air pollution, the quality indicators of the pollen of the bioindicator remain high, within 77.79 % (test site No. 1, which is located closest to the highways, where Nox is 2.11 g/s, CH – 2,86 g/s) to 81.77 % (test site No. 2 in Mariinsky Park, which has the best environmental conditions: the mixture of nitrogen oxides is 0.41, which is almost five times less than at test site No. 1 in Peremohy Park). The largest share of teratomorphic pollen (34.98 %) was recorded in T. officinalis plants in Peremogy Park test site No. 1, the smallest (26.86 %) was found in plants growing in Mariinsky Park at test site No. 2. When analyzing the data, it was determined that a high level of inverse correlation has an indicator of the fertility of pollen grains with all investigated components of air pollution (r = from -0.57 – Pb compounds to -0.73 – soot). Conclusions. Therefore, the qualitative indicators of the pollen of the phytoindicator T. officinalis can be recommended for use in the system of ecological monitoring of the environment with different degrees of aerogenic load.","PeriodicalId":475640,"journal":{"name":"Faktori eksperimentalʹnoï evolûcìï organìzmìv","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135427920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of free proline accumulation on the content of photosynthetic pigments in transgenic wheat plants 游离脯氨酸积累对转基因小麦光合色素含量的影响

Faktori eksperimentalʹnoï evolûcìï organìzmìv Pub Date : 2023-09-01 DOI: 10.7124/feeo.v33.1578

O. V. Dubrovna, G. O. Priadkina

{"title":"The effect of free proline accumulation on the content of photosynthetic pigments in transgenic wheat plants","authors":"O. V. Dubrovna, G. O. Priadkina","doi":"10.7124/feeo.v33.1578","DOIUrl":"https://doi.org/10.7124/feeo.v33.1578","url":null,"abstract":"Aim. To analyze the effect of L-proline accumulation on chlorophyll content in transgenic wheat plants with a double-stranded RNA suppressor of the proline dehydrogenase gene under physiological and stress conditions. Methods. Agrobacterium-mediated transformation in planta; biochemical determination of free proline content; spectrophotometric determination of photosynthetic pigments content; of mathematical statistics. Results. It was shown that the content of free proline in the leaves of transgenic plants under physiological conditions was 1.7-1.9 times higher compared to the original genotype. Under conditions of drought, the content of this aminoacid increased in non-transformed plants of the original genotypes by 2.9-3.1 times, and in transgenic plants – by 4.5-4.9 times. The amount of chlorophyll in the flag leaves of plants of the original genotypes and their transgenic lines under physiological conditions did not differ significantly, while under drought conditions in the first of them it was 1.1-1.2 times less than in the second. Drought reduced the total chlorophyll content in plants of the original genotype to 85-90 %, compared to physiological conditions, while no significant changes were found in transgenic plants. Conclusions. It was established that under conditions of soil drought, the increase in proline content in genetically modified wheat plants compared to non-transgenic ones is accompanied by an increase in the amount of total chlorophyll (by 10-15 %), which indicates a better efficiency of their pigment apparatus under stressful conditions.","PeriodicalId":475640,"journal":{"name":"Faktori eksperimentalʹnoï evolûcìï organìzmìv","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135428135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dependency of chlorophyll fluorescence of in vitro plant of Carlina L. on light conditions during their cultivation 柠条离体植株叶绿素荧光对光照条件的依赖性

Faktori eksperimentalʹnoï evolûcìï organìzmìv Pub Date : 2023-09-01 DOI: 10.7124/feeo.v32.1543

Kh. M. Kolisnyk, L. R. Hrytsak, M. Z. Prokopiak, N. M. Drobyk

{"title":"Dependency of chlorophyll fluorescence of in vitro plant of Carlina L. on light conditions during their cultivation","authors":"Kh. M. Kolisnyk, L. R. Hrytsak, M. Z. Prokopiak, N. M. Drobyk","doi":"10.7124/feeo.v32.1543","DOIUrl":"https://doi.org/10.7124/feeo.v32.1543","url":null,"abstract":"Aim. To investigate the peculiarities of the functioning of the photosynthetic apparatus of plants of Carlina L. species under different light conditions of in vitro cultivation and under natural conditions using the method of induction of fluorescence of chlorophyll a. Methods. Methods of in vitro plants cultivation, a method of inducing fluorescence of chlorophyll a in light-adapted leaves. Results. It has been demonstrated that in vitro conditions reflect the evolved light growth requirements of species. Our previously obtained results regarding a closer match of physiological needs of in vitro plants of the species of Carlina onopordifolia Besser ex Szafer, Kulcz. et Pawt. to light conditions of variant 1.1 have been confirmed. Based on the synthesis of the dynamics of photosynthetic pigment content and their ratios in in vitro plants of Carlina acaulis L., as well as key parameters of fluorescence under different cultivation light conditions, a conclusion has been drawn about the greater alignment of variant 1.1 with the needs of plants of this species. It is hypothesized that in natural conditions, plants of the species of C. onopordifolia and Carlina cirsioides Klokov undergo abiotic stresses, manifested in increased dissipation of light energy into heat and the processes of photo-inhibition. There is significant enhancement in electron transport within the light-harvesting complex and reduction in the efficiency of quantum yield and light energy storage by Photosystem II. The vitality index of these species in their natural growth conditions is found to be half of the commonly accepted optimum value and 1.97–2.96 times lower compared to experimental in vitro plant groups. Conclusions. The obtained results suggest the usefulness of using the chlorophyll fluorescence induction method for assessing the functioning of the photosynthetic apparatus of in vitro plants of the genus Carlina.","PeriodicalId":475640,"journal":{"name":"Faktori eksperimentalʹnoï evolûcìï organìzmìv","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135428137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study of trinucleotide CAG-repeats expansion in androgen receptor gene among patients with suspected Kennedy’s syndrome 疑似肯尼迪综合征患者雄激素受体基因三核苷酸cag -重复序列扩增的研究

Faktori eksperimentalʹnoï evolûcìï organìzmìv Pub Date : 2023-09-01 DOI: 10.7124/feeo.v33.1575

B. I. Tretiak, M. Ya. Tyrkus, Kh. Ya. Bakum, D. V. Zastavna, H. R. Akopyan

引用次数: 0

Genetic analysis of sulfate assimilation gene cluster of Streptomyces coelicolor A3(2) 色链霉菌A3硫酸盐同化基因簇的遗传分析(2)

Faktori eksperimentalʹnoï evolûcìï organìzmìv Pub Date : 2023-09-01 DOI: 10.7124/feeo.v32.1537

B. O. Ostash, K. Stanchak, T. Gren, V. O. Fedorenko

{"title":"Genetic analysis of sulfate assimilation gene cluster of Streptomyces coelicolor A3(2)","authors":"B. O. Ostash, K. Stanchak, T. Gren, V. O. Fedorenko","doi":"10.7124/feeo.v32.1537","DOIUrl":"https://doi.org/10.7124/feeo.v32.1537","url":null,"abstract":"Aim. Streptomyces coelicolor A3(2) is the best studied species within this bacterial genus. Biosynthesis of specialized (secondary) metabolites by Streptomyces is of special interest. Primary metabolism, where all the precursors of specialized metabolites come from, is also studied in great detail. There are glaring gaps in our knowledge of sulfur metabolism in this species. We took genetic approach to probe the function of several genes within presumed sulfate assimilation gene cluster of S. coelicolor A3(2). Methods. Microbiological and genetic approaches were combined to generate mutants and to study their properties. Results. Sulfate assimilation gene cluster is structurally and functionally similar to that of phylogenetically close Corynebacterium. Most of the generated knockout strains behaved as would be expected from their molecular function inferred in silico. This confirms their involvement in sulfate uptake/conversion. Knockout of gene sco6101 (having no homologs from the other bacterial sulfate assimilation operons) impaired the growth on inorganic sulfur species and L-cysteine, pointing to its association with sulfur metabolism. Conclusions. Our study provides experimental evidence for the involvement of sco6093-sco6102 segment in sulfate assimilation, and also reveals novel gene, sco6101, essential for sulfur cycle. Further efforts are needed to elucidate the mechanism of Sco6101 action.","PeriodicalId":475640,"journal":{"name":"Faktori eksperimentalʹnoï evolûcìï organìzmìv","volume":"60 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135428486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cytogenetic studies in reproductive function disorders: a retrospective analysis 生殖功能障碍的细胞遗传学研究:回顾性分析

Faktori eksperimentalʹnoï evolûcìï organìzmìv Pub Date : 2023-09-01 DOI: 10.7124/feeo.v33.1571

N. L. Huleyuk, H. M. Bezkorovaina, D. V. Zastavna

{"title":"Cytogenetic studies in reproductive function disorders: a retrospective analysis","authors":"N. L. Huleyuk, H. M. Bezkorovaina, D. V. Zastavna","doi":"10.7124/feeo.v33.1571","DOIUrl":"https://doi.org/10.7124/feeo.v33.1571","url":null,"abstract":"Aim. Fertility disorders are one of the most common problems of reproductive medicine. Chromosomal abnormalities play a crucial role in reproductive failure. The main purpose of our study was to determine the types and frequency of chromosomal aberrations in couples with history of infertility. Methods. Karyotyping was performed in 652 couples with reproductive failure between 2004 and 2022, including 203 couples with primary infertility (PI), 240 couples with recurrent pregnancy loss (RPL) and 209 – with sporadic pregnancy loss (SPL). Results. Numerical and structural karyotype abnormalities were detected in 37 (2.84 %) of 1304 individuals with infertility, most frequently in group with PI – 3.4 % and RPL – 3.3 %. Chromosomal abnormalities were detected in 3.1 % woman and 2.6 % men. The most common cytogenetic abnormalities (26 cases of 37; 2 %) are translocations, reciprocal – 18 cases (1.4 %) and Robertsonian – 8 cases (0.6 %). Numerical and structural gonosomal abnormalities were detected in 9 individuals (0.7 %), supernumerary marker chromosome – in 2 (0.14 %). Conclusions. Karyotype abnormalities are most common in individuals with PI and RPL and a occur with the same frequency among women and men. Numerical gonosomal abnormalities were detected only as mosaic form.","PeriodicalId":475640,"journal":{"name":"Faktori eksperimentalʹnoï evolûcìï organìzmìv","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135428490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gender pecularity of the material of reproductive losses 性别特殊性的物质生殖损失

Faktori eksperimentalʹnoï evolûcìï organìzmìv Pub Date : 2023-09-01 DOI: 10.7124/feeo.v33.1574

I. R. Tkach, N. L. Huleyuk, G. M. Bezkorovaina, N. V. Helner, T. B. Snizhko, O. V. Benko

引用次数: 0