Faktori eksperimentalʹnoï evolûcìï organìzmìv最新文献_第7页

Analysis of Atg proteins involved in the formation of the Atg1 complex, their interaction with the Atg8 protein during autophagosome maturation 分析参与Atg1复合物形成的Atg蛋白及其在自噬体成熟过程中与Atg8蛋白的相互作用

Faktori eksperimentalʹnoï evolûcìï organìzmìv Pub Date : 2023-09-01 DOI: 10.7124/feeo.v32.1550

E. V. Bulgakov, O. V. Rayevsky

{"title":"Analysis of Atg proteins involved in the formation of the Atg1 complex, their interaction with the Atg8 protein during autophagosome maturation","authors":"E. V. Bulgakov, O. V. Rayevsky","doi":"10.7124/feeo.v32.1550","DOIUrl":"https://doi.org/10.7124/feeo.v32.1550","url":null,"abstract":"Aim. Relying on the data from numerous researches on the representatives of ATG proteins from H.sapiens and S.cerevisiae organisms, we decided to explore the difference between ATG proteins from plant organism. Subsequent stages comprised the determination of in silico phosphorylation effects on the native structure of the protein model and the possible influences on the stability of the three-dimensional complex by the molecular dynamics method. Methods. Methodologically, one can highlight the process of models elaboration on known sequences in AlphaFold 2.0 program and subsequent mechanistic review of molecular mobility of the obtained conformational models through the molecular dynamics in Gromacs 4.5 program and Charmm36 force field. Results. As a result of the model development and exploring the process of ATG-protein complexation, the geometrical specificities of the structures under investigation revealed the binding sites on the surface of the Atg1 complex components. We also suggested some areas for the intermolecular interactions based on the already published data on experimentl mutagenesis. Additively, we performed an analysis of the molecular dynamics simulations in the case of phosphorylation and its effect on the conformational mobility of these objects. Conclusions. Applying the computer simulation modelling methods we created the Atg1 complex elements and then analyzed these four proteins participating in the process.","PeriodicalId":475640,"journal":{"name":"Faktori eksperimentalʹnoï evolûcìï organìzmìv","volume":"372 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135427879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Properties of actinomycetes from the rhizosphere of Colobanthus quitensis (Kunth) Bartl. (Booth Island, maritime Antarctica) 香竹根际放线菌的特性研究。(南极海上的布斯岛)

Faktori eksperimentalʹnoï evolûcìï organìzmìv Pub Date : 2023-09-01 DOI: 10.7124/feeo.v33.1584

І. І. Roman, І. Y. Parnikoza, V. Y. Syrvatka, V. O. Fedorenko, О. М. Gromyko

{"title":"Properties of actinomycetes from the rhizosphere of Colobanthus quitensis (Kunth) Bartl. (Booth Island, maritime Antarctica)","authors":"І. І. Roman, І. Y. Parnikoza, V. Y. Syrvatka, V. O. Fedorenko, О. М. Gromyko","doi":"10.7124/feeo.v33.1584","DOIUrl":"https://doi.org/10.7124/feeo.v33.1584","url":null,"abstract":"Aim. Investigate the biological properties of actinomycetes from the rhizosphere of Colobanthus quitensis (Kunth) Bartl. and study their ability to produce bioactive compounds. Methods. Microbiological (isolation, synthesis of bioactive compounds), genetic and genetic engineering (isolation and analysis of total DNA, DNA gel electrophoresis, polymerase chain reaction, DNA sequencing), bioinformatic (phylogenetic analysis) methods. Results. 21 actinomycete isolates were obtained from the samples of C. quitensis rhizosphere. Half of the isolates exhibited antagonistic properties towards at least one of 17 test cultures of pathogenic and phytopathogenic microorganisms. Some strains exhibited both antibacterial and antifungal activities. The majority of isolates (70-95 %) produced proteases, amylases, cellulases, almost 42 % – nitrate reductases, 20-25 % – pectinases and lipases, respectively. Conclusions. A collection of Antarctic actinomycetes has been created and characterized, which is promising for evaluating their metabolic potential as producers of antibiotics.","PeriodicalId":475640,"journal":{"name":"Faktori eksperimentalʹnoï evolûcìï organìzmìv","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135428301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Features of the expression of breeding traits in Linum usitatissimum L. somaclones 羊草体细胞无性系育种性状的表达特征

Faktori eksperimentalʹnoï evolûcìï organìzmìv Pub Date : 2023-09-01 DOI: 10.7124/feeo.v32.1546

S. V. Mishchenko, L. M. Kryvosheeva, M. V. Sribnyi

{"title":"Features of the expression of breeding traits in Linum usitatissimum L. somaclones","authors":"S. V. Mishchenko, L. M. Kryvosheeva, M. V. Sribnyi","doi":"10.7124/feeo.v32.1546","DOIUrl":"https://doi.org/10.7124/feeo.v32.1546","url":null,"abstract":"Aim. Determining the level of expression, variability and features of inheritance of the main breeding traits of flax somaclones (Linum usitatissimum L.) in connection with the possibility of their use as initial selection material was the aim of our research. Methods. Hypocotyl and epicotyl segments for the induction of callusogenesis and somatic embryogenesis were cultured in vitro on the nutrient medium of Murashige and Skoog with the addition of 1.00 mg/l 6-benzylaminopurine and 0.05 mg/l 1-naphthylacetic acid, 30 g/l sucrose, a photoperiod of 16 h, illumination 2500 lux, relative air humidity 60–80 %, air temperature 22–24°C. Somaclones were adapted in vivo, and their generations were studied in the field. Results. Somaclones of the Hlinum variety were tested in the field according to the main selection traits and were not significantly inferior to the control variant; however, among them it is possible to single out some valuable individual plants for further breeding. Conclusions. Peculiarities of correlations between traits indicate the possibility of creating breeding material with a high level of fibrousness and seed productivity at the same time, high fiber mass and its content, allow selection for fibrousness by indirect traits – total and/or technical stem length, stem mass. A promising direction is the use of somaclones as sources of a high level of manifestation of the trait of the number of capsules on a plant (up to 17 pcs.).","PeriodicalId":475640,"journal":{"name":"Faktori eksperimentalʹnoï evolûcìï organìzmìv","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135428492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic analysis of the content of palmytic acid glycerides in the carriers of maize mutation shrunken-1 玉米突变体萎缩1号携带者棕榈酸甘油酯含量的遗传分析

Faktori eksperimentalʹnoï evolûcìï organìzmìv Pub Date : 2023-09-01 DOI: 10.7124/feeo.v33.1566

D. S. Tymchuk

{"title":"Genetic analysis of the content of palmytic acid glycerides in the carriers of maize mutation shrunken-1","authors":"D. S. Tymchuk","doi":"10.7124/feeo.v33.1566","DOIUrl":"https://doi.org/10.7124/feeo.v33.1566","url":null,"abstract":"Aim. The influence of corn shrunken-1 (sh1) mutation on the content of palmitate in corn oil establishing and genetic analysis of this trait. Methods. The effects of the sh1 mutation on the content of palmitate were determined by comparing the inbreds – carriers of this mutation with inbreds of the common type, as well as in the top-crosses of common type inbreds with the mutant inbreds. The genetic components of the variance in terms of the content of palmitate were analyzed in the diallel crosses of mutant inbreds according to the second Griffing method. The determination of oil fatty acid composition was carried out by the Peisker gas chromatographic method. Results. It has been established that the corn inbreds – carriers of sh1 mutation exceed the common type inbreds by an average of 29.2 % in terms of palmitate content. The level of the trait in the inbreds based on the sh1 mutation was notable as having the quantitative variability and varied within 12.2-15.6 %. The content of palmitate in the carriers of sh1 mutation was inherited as incomplete dominance with a predominant contribution of additive effects to the variance. A higher level of the trait was controlled by recessive alleles of polygenes. Conclusions. The obtained results suggests the possibility of spatial linkage of the sh1 mutant gene with palmitate-coding locus, the effect of which is modified by the polygenic complex. It has been confirmed, that the carriers of sh1 mutation expands the genetic diversity of corn in terms of palmitate content.","PeriodicalId":475640,"journal":{"name":"Faktori eksperimentalʹnoï evolûcìï organìzmìv","volume":"62 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135427903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative analysis of Camelina sativa and fungal industrial lipases used for biodiesel production 用于生物柴油生产的亚麻荠与真菌工业脂肪酶的比较分析

Faktori eksperimentalʹnoï evolûcìï organìzmìv Pub Date : 2023-09-01 DOI: 10.7124/feeo.v32.1530

V. Y. Hotsuliak, R. Y. Blume, Y. B. Blume

引用次数: 0

Prevalence of PAI-1 675 4G/5G and ACE I/D polymorphic variants in women with recurrent pregnancy losses PAI-1 675 4G/5G和ACE I/D多态性变异在复发性妊娠丢失妇女中的患病率

Faktori eksperimentalʹnoï evolûcìï organìzmìv Pub Date : 2023-09-01 DOI: 10.7124/feeo.v33.1576

L. B. Chorna, D. V. Zastavna, H. V. Makukh, O. I. Kolodiy

{"title":"Prevalence of PAI-1 675 4G/5G and ACE I/D polymorphic variants in women with recurrent pregnancy losses","authors":"L. B. Chorna, D. V. Zastavna, H. V. Makukh, O. I. Kolodiy","doi":"10.7124/feeo.v33.1576","DOIUrl":"https://doi.org/10.7124/feeo.v33.1576","url":null,"abstract":"Aim. The present study aimed to assess the distribution of inherited risk factors of thrombophilia among women with RPL. To determine the frequency and distribution of alleles and genotypes of polymorphic loci PAI-1 6754G/5G and ACE I/D among women with RPL. Methods. The study was carried out in a group of 97 women (57 controls and 40 women with RPL). Polymerase chain reaction and restriction fragment length polymorphism (RFLP) were used to identify the polymorphisms. Results. The frequency of 4G allele of PAI-1 675 5G/4G locus was more frequent in the group of women with RPL – 67 %, compared to 54 % in the control group. It was established that the presence of the 4G allele increases the risk of RPL by almost 2 times (Р = 0.01). In the group of women with RPL the frequency of carriers of DD genotype and D allele of ACE gene was higher than in the control group (31 % vs. 19 %), but no significant differences were found. The frequency of women with a combination of 4G4G and DD genotypes of the two studied loci was 16.6 % in the group with RPL versus 2.3 % in the control group. It was shown that the presence of homozygotes for two alleles 4G of the PAI-1 gene and D of the ACE gene leads to an 8-fold increased risk of RPL (OR = 8.6, CI: 0.9841-75.154, P = 0.04). Conclusions. Significance of PAI-1 675 4G/5G and ACE I/D polymorphic variants, especially in combination, in the predisposition to RPL was established.","PeriodicalId":475640,"journal":{"name":"Faktori eksperimentalʹnoï evolûcìï organìzmìv","volume":"59 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135428496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0