Faktori eksperimentalʹnoï evolûcìï organìzmìv最新文献_第6页

Identification of mutations related to phytate accumulation in barley kernels 大麦籽粒植酸积累相关突变的鉴定

Faktori eksperimentalʹnoï evolûcìï organìzmìv Pub Date : 2023-09-01 DOI: 10.7124/feeo.v32.1532

V. B. Katrii, L. G. Velikozhon, L. V. Slivka, B. V. Morgun

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Cytogenetic disorders of winter wheat root meristem cells under the action of moderate and high concentrations of N-nitroso-N-methylurea 中、高浓度n -亚硝基-n -甲基脲作用下冬小麦根分生组织细胞的细胞遗传学紊乱

Faktori eksperimentalʹnoï evolûcìï organìzmìv Pub Date : 2023-09-01 DOI: 10.7124/feeo.v32.1529

R. A. Yakymchuk

{"title":"Cytogenetic disorders of winter wheat root meristem cells under the action of moderate and high concentrations of N-nitroso-N-methylurea","authors":"R. A. Yakymchuk","doi":"10.7124/feeo.v32.1529","DOIUrl":"https://doi.org/10.7124/feeo.v32.1529","url":null,"abstract":"Aim. To study the frequency and spectrum of the cytogenetic disorders in Triticum aestivum L., induced by the effect of moderate and high concentrations of N-nitroso-N-methylurea. Methods. The seeds of winter wheat of cultivars Albatros odeskyi and Zymoiarka were treated with water solution of N-nitroso-N-methylurea in such concentrations as 0.005 %, 0.01 %, 0.025 %. A cytogenetic analysis of the cells of a root meristem was made with help of an anatelophase method. Results. The frequency of mitoses with chromosome aberrations amounted to 2.74–23.05 %, and it depended on the concentration of a mutagen and a plant genotype. A significant increase of the frequency of the aberrant cells was recorded as a result of the effect of the mutagen in the concentration 0.025 %, which was associated with a radiometric impact caused by the influence of a chemical mutagen in a high concentration. The spectrum of cytogenetic disorders contained mostly acentric fragments, the bridges of a chromatid type and the chromosomes which lagged behind. As the super-mutagen concentration increased, the spectrum expansion of the cytogenetic disorders took place at the expense of the even acentric fragments, micro-nuclei and acentric rings. Conclusions. The frequency of the chromosome aberrations, induced by the effect of NMU, exceeds the control level by 3.5–39.7 times, and it depends directly on the concentration of a chemical mutagen. N-nitroso-N-methylurea in the concentration equal to 0.025 % shows a radiometric effect which classifies a mentioned amount of the concentration of a super-mutagen as a high one. A serious increase of the indicator of the number of aberrations per aberrant cell appeared to be typical only when it was under the effect of a chemical mutagen in the highest concentration.","PeriodicalId":475640,"journal":{"name":"Faktori eksperimentalʹnoï evolûcìï organìzmìv","volume":"32 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135428288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Selected abstracts of reports at the XVІІІ Intrnational Scientific Conference «Factors in Experimental Evolution of Organisms» 在XVІІІ国际科学会议«生物实验进化的因素»报告的精选摘要

Faktori eksperimentalʹnoï evolûcìï organìzmìv Pub Date : 2023-09-01 DOI: 10.7124/feeo.v33.1593

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Research of descendants of transgenic plants Triticum aestivum L. with partial suppression of the prolinde hydrogenase gene 脯氨酸氢化酶基因部分抑制转基因小麦后代的研究

Faktori eksperimentalʹnoï evolûcìï organìzmìv Pub Date : 2023-09-01 DOI: 10.7124/feeo.v32.1544

A. G. Komisarenko, S. I. Mykhalska

{"title":"Research of descendants of transgenic plants Triticum aestivum L. with partial suppression of the prolinde hydrogenase gene","authors":"A. G. Komisarenko, S. I. Mykhalska","doi":"10.7124/feeo.v32.1544","DOIUrl":"https://doi.org/10.7124/feeo.v32.1544","url":null,"abstract":"Aim. To investigate the level of tolerance to water deficit of seed generations (T1 and T4) of genetically modified winter wheat with partially suppressed expression of the proline dehydrogenase gene (ProDH) based on the analysis of physiological and biochemical indicators and economic characteristics of plants. Methods. Determination of indicators of crop structure and content of free L-proline (Pro). Results. The level of Pro was studied and the main elements of productivity in the offspring of transgenic plants and their original forms under normal and insufficient water supply were analyzed. Conclusions. T1 and T4 biotechnological plants under normal/stress conditions accumulated more Pro than the original genotypes. The level of this amino acid in genetically modified seedlings under optimal cultivation conditions exceeded the initial forms by an average of 1.8 times. Under the influence of water deficit, its content increased by 2.2 and 2.3 times, in relation to normal water supply. In non-transgenic variants, the level of Pro, under similar growing conditions, was lower by 1.9 and 2.0 times, compared to T1 and T4 plants. Under the optimal water regime, the analyzed wheat variants did not differ significantly in terms of productivity. Drought led to their decrease, but the offspring of biotechnological plants were characterized by a less pronounced difference, as well as a higher yield.","PeriodicalId":475640,"journal":{"name":"Faktori eksperimentalʹnoï evolûcìï organìzmìv","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135428628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Algorithm for creating ILP markers for molecular genetic analysis of Triticum durum and other types of cereals 用于硬粒小麦和其他谷物分子遗传分析的ILP标记创建算法

Faktori eksperimentalʹnoï evolûcìï organìzmìv Pub Date : 2023-09-01 DOI: 10.7124/feeo.v32.1536

D. O. Novozhylov, S. O. Hordynskyi, A. S. Postovoitova, A. M. Rabokon, Ya. V. Pirko, Y. B. Blume

{"title":"Algorithm for creating ILP markers for molecular genetic analysis of Triticum durum and other types of cereals","authors":"D. O. Novozhylov, S. O. Hordynskyi, A. S. Postovoitova, A. M. Rabokon, Ya. V. Pirko, Y. B. Blume","doi":"10.7124/feeo.v32.1536","DOIUrl":"https://doi.org/10.7124/feeo.v32.1536","url":null,"abstract":"Aim. Development of an algorithm for creating ILP markers suitable for breeding studies of durum wheat (Triticum durum) and some other types of cereals. Methods. Use of classical bioinformatics tools to locate potential intron sites of target genes in T. durum suitable for use as ILP markers based on homology of T. durum contigs and rice (Oryza sativa) coding sequences. Analysis of exon-intron structure of homologous genes in O. sativa, Hordeum vulgare, Aegilops tauschii and Triricum aestivum followed by selection of appropriate primers for potential ILP markers. Results. A number of potential location sites of target introns in T. durum contig sequences were identified. Degenerate primers were designed for them, taking into account the analysis of the exon-intron structure of the corresponding homologous genes in O. sativa, H. vulgare, A. tauschii and T. aestivum. Conclusions. An algorithm for the creation of ILP markers was developed, which can be used in molecular genetic studies of plants in the case of the absence or incomplete sequenced genome of the studied species and the lack of information about the exon-intron structure of their genes. A number of potential ILP markers that can be used for cereals, in particular T. durum, have been identified.","PeriodicalId":475640,"journal":{"name":"Faktori eksperimentalʹnoï evolûcìï organìzmìv","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135428123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Study of variability alpha-amylase isozymes in wheat varieties of Ukrainian breeding 乌克兰小麦品种α -淀粉酶同工酶变异研究

Faktori eksperimentalʹnoï evolûcìï organìzmìv Pub Date : 2023-09-01 DOI: 10.7124/feeo.v33.1565

N. V. Sandetska, O. M. Radchenko, I. N. Sheheda, M. O. Dykun, L. V. Sirant

{"title":"Study of variability alpha-amylase isozymes in wheat varieties of Ukrainian breeding","authors":"N. V. Sandetska, O. M. Radchenko, I. N. Sheheda, M. O. Dykun, L. V. Sirant","doi":"10.7124/feeo.v33.1565","DOIUrl":"https://doi.org/10.7124/feeo.v33.1565","url":null,"abstract":"Aim. To investigate the variability of alpha-amylase isoenzymes in soft wheat varieties of Ukrainian selection, created by breeding institutions located in different climatic zones (Forest Steppe and Steppe). Methods. Isoenzymes of alpha-amylase were detected by electrophoretic protein separation in a polyacrylamide gel. The fall number on the «Falling Number 1700» was determined. Results. For a representative assessment of the distribution of alpha-amylase isoenzymes, we studied the zoned varieties of common wheat from two breeding centers located in different climatic zones: the Institute of Plant Physiology and Genetics of the National Academy of Sciences of Ukraine, Kyiv (Forest-steppe) and the Breeding and Genetics Institute of the National Academy of Sciences, Odessa (Steppe). The variability of wheat grain alpha-amylase was revealed. A search for associations between alpha-amylase variants and resistance to pre-harvest grain germination was conducted. Conclusions. The prevalence of alpha-amylase isoenzyme variants in Ukrainian common wheat varieties was estimated. Among Odessa varieties, the AbCd alpha-amylase variant predominates with a frequency of 50.0 %. AbCd and AbCD phenotypes with a frequency of 38.8 % predominate in the selection varieties of the IFRG of the National Academy of Sciences of Ukraine. It was shown that the genotypes containing the AbCD and ABсD isoenzyme variant are more resistant to pre-harvest germination, and the ABCd and AbcD variants are less resistant to pre-harvest grain germination.","PeriodicalId":475640,"journal":{"name":"Faktori eksperimentalʹnoï evolûcìï organìzmìv","volume":"99 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135427369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mexican argemone (Argemone mexicana L.) CYP719A14 protein bioinformatic analysis 墨西哥银盖酮(argemone mexicana L.)CYP719A14蛋白生物信息学分析

Faktori eksperimentalʹnoï evolûcìï organìzmìv Pub Date : 2023-09-01 DOI: 10.7124/feeo.v32.1553

H. Slishchuk, V. I. Meshcheryakov, N. E. Volkova, O. A. Zakharova

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Falz-Fein Biosphere Reserve "Askania-Nova" (dedicated to the 125th foundation anniversary) 法尔兹-费恩生物圈保护区“阿斯卡尼亚-新星”(纪念成立125周年)

Faktori eksperimentalʹnoï evolûcìï organìzmìv Pub Date : 2023-09-01 DOI: 10.7124/feeo.v32.1557

I. B. Chen, H. B. Humeniuk, S. O. Yastremska

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The content of soluble carbohydrates and proteins in the seedlings of soybean varieties with contrast photoperiodic response under the influence of selective light 选择性光影响下具有对比光周期响应的大豆品种幼苗可溶性碳水化合物和蛋白质含量

Faktori eksperimentalʹnoï evolûcìï organìzmìv Pub Date : 2023-09-01 DOI: 10.7124/feeo.v33.1585

Y. D. Batuieva, O. O. Avksentieva

{"title":"The content of soluble carbohydrates and proteins in the seedlings of soybean varieties with contrast photoperiodic response under the influence of selective light","authors":"Y. D. Batuieva, O. O. Avksentieva","doi":"10.7124/feeo.v33.1585","DOIUrl":"https://doi.org/10.7124/feeo.v33.1585","url":null,"abstract":"Aim. Study of the influence of selective light irradiation on the content of soluble carbohydrates and proteins in the axial organs of seedlings of soybean varieties with a contrasting photoperiodic response (PPR). Methods. As plant material we used etiolated seedlings of soybean (Glycine max (L.) Merr.) SDP Khadjibei variety and DNP Yatran variety. Etiolated seedlings were irradiated daily for 30 minutes (5 days) using LED matrices with red, green and blue light. Then the material was fixed and a biochemical analysis was carried out in the axial organs of the seedlings – the content of soluble carbohydrates and proteins was determined. Results. GL and BL irradiation had the maximum effect on the content of soluble carbohydrates in SDP soybean seedlings of Khadjibey variety. In soybean seedlings of the DNP Yatran variety a significant effect was exerted by the action of all studied spectra, which was manifested in a decrease in their content in the above-ground part and growth in the root system. The content of proteins in seedlings of both varieties under the influence of selective light increased in both parts of the seedling. The most significant effect was observed for the irradiation of GL. Conclusions. The effects of selective light irradiation are manifested differently in shoots and roots, which may be due to the different composition or activity of the photoreceptor systems of the axial organs.","PeriodicalId":475640,"journal":{"name":"Faktori eksperimentalʹnoï evolûcìï organìzmìv","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135428634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Surnames in population genetics: pros and cons 人口遗传学中的姓氏:利与弊

Faktori eksperimentalʹnoï evolûcìï organìzmìv Pub Date : 2023-09-01 DOI: 10.7124/feeo.v32.1554

L. O. Atramentova

{"title":"Surnames in population genetics: pros and cons","authors":"L. O. Atramentova","doi":"10.7124/feeo.v32.1554","DOIUrl":"https://doi.org/10.7124/feeo.v32.1554","url":null,"abstract":"The article presents arguments regarding the perspective of studying the population using surnames as a tool of population genetic research. The model of a virtual polyallelic locus linked to the Y chromosome in peoples with patrilineal inheritance and linked to mtDNA in peoples with matrilineal inheritance is presented, which allows analyzing the distribution of surnames by means of population genetics. It is proved that the change of surnames at the time of marriage, false paternity and spelling errors do not affect the overall result of the calculations. Attention is drawn to the selective neutrality of surnames in relation to the natural environment and selectivity to the social environment. Examples of the advantages of surnames as population markers are given: quick obtaining of results, low financial cost of works compared to effectiveness, absence of statistical and ethical problems. The expediency of using surnames for the study of Ukrainian populations is proven by their compliance with the conditions put forward for markers. In conditions that make it impossible to collect actual material in populations and laboratory analysis of biological samples, working with lists of surnames is best suited for undergraduates and graduate students conducting research in anthropogenetics.","PeriodicalId":475640,"journal":{"name":"Faktori eksperimentalʹnoï evolûcìï organìzmìv","volume":"58 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135427654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0