Natural and Life Sciences Communications最新文献

{"title":"Optimization of HPMC-Based Oral Fast Dissolving Film of Cetirizine Dihydrochloride","authors":"Kamchai Saepang, T. Pitaksuteepong, Benjaporn Buranrat, Supavadee Boontha","doi":"10.12982/nlsc.2024.007","DOIUrl":"https://doi.org/10.12982/nlsc.2024.007","url":null,"abstract":"There are challenges involved in improving the convenience of cetirizine dihydrochloride (CTZ) administration for geriatric and pediatric patients with allergic asthma. Due to the ease of swallowing, oral fast-dissolving films (OFDFs) have been identified as a potential solution. This study aimed to prepare hydroxypropyl methylcellulose (HPMC)-based OFDFs of CTZ using the solvent casting method. Film compositions, including the amounts of HPMC and glycerin, were optimized using a face-centered central composite design based on film thickness, folding endurance (FE), and disintegration time (DT). Nine prepared films were found to be transparent and clear in appearance, demonstrating the homogeneity of the films. The weights and thicknesses of all films were in the ranges of 57-109 mg and 22 - 40 µm. The FE of the prepared films was determined to be 42-156, 30-124, and 17-70 times for 3%, 4%, and 5% (w/w) HPMC-based films, respectively. The DT of the films ranged from 99 ± 13 to 478 ± 21 s as their compositions were altered in terms of the HPMC contents and the amount of glycerin. The film fabricated from 3 % (w/w) HPMC and 10% (w/w polymer mass) glycerin (F3) was identified as optimal due to achieving the highest FE and the least DT. Moreover, regression analysis demonstrated a significant effect of the HPMC and glycerin amounts on the film properties. After adding CTZ in F3 (the optimized film), the CTZ content in the films was 0.84 ± 0.14 mg/cm2. The optimized film could be evaluated further for drug release and drug permeation. Keywords: Cetirizine dihydrochloride, Film former, Folding endurance, HPMC-based film, Plasticizer","PeriodicalId":471315,"journal":{"name":"Natural and Life Sciences Communications","volume":"135 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139453141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design and Optimization of Ganciclovir Nanosuspension Loaded In Situ Gelling Mucoadhesive Eye Drops for Herpetic Keratitis","authors":"Phuvamin Suriyaamporn, Boonnada Pamornpathomkul, Theerasak Rojanarata, Prasopchai Patrojanasophon, Praneet Opanasopit, Tanasait Ngawhirunpat","doi":"10.12982/nlsc.2023.068","DOIUrl":"https://doi.org/10.12982/nlsc.2023.068","url":null,"abstract":"Ganciclovir (GCR), an antiviral drug used to treat herpetic keratitis, is less effective because of its poor bioavailability (BCS III). Nanosuspension (NS) is a promising technique for improving solubility and the dissolution of poorly soluble drugs by size reduction. Topical solution or conventional eye drop represents the easiest route to deliver drugs to the anterior eye segment; however, low precorneal retention, high nasolacrimal drainage, and metabolic degradation lead to low bioavailability. To overcome these limitations, incorporating GCR-NS into mucoadhesive in situ gel-forming (ISG) could enhance its ocular permeability and drug bioavailability. Therefore, this study aimed to design and optimize GCR-NS-loaded in situ gelling mucoadhesive eye drops (GCR-NS-loaded mucoadhesive ISG) for improving ophthalmic delivery. Pluronic® F127 (P127), an ophthalmic gel forming, was selected as a polymer due to self-gelling formation by temperature-triggered in situ gelling at ocular temperature. Moreover, hyaluronic acid-modified catechol (HA-cat), a novel mucoadhesive polymer, was combined to achieve the desired ocular mucoadhesion and facilitate ophthalmic delivery. Optimized GCR-NS formulation prepared using the nanoprecipitation technique was selected based on the central composite design (CCD) model by evaluating their particle size, polydispersity index (PDI), and zeta potential, before being loaded into mucoadhesive ISG. The optimal GCR-NS-loaded mucoadhesive ISG (F7) revealed the desired physicochemical properties with better viscosity, mucoadhesion, and gelling capacity at physiological conditions, high ocular permeation with a sustained manner over 24 h compared to eye drop suspension. Accordingly, GCR-NS-loaded mucoadhesive ISG could be a promising ocular delivery system for the effective local delivery of GCR for herpetic keratitis. Keywords: Ganciclovir, Nanosuspension, Mucoadhesive eye drop, Ophthalmic delivery, In situ gelling","PeriodicalId":471315,"journal":{"name":"Natural and Life Sciences Communications","volume":"11 16","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135813352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bloom Performances during Rainy Season of Off-season Longan (Dimocarpus longan Lour. ‘Phuang Thong’) Grown at Samut Sakhon Province, Thailand","authors":"Phyu Phyu Aung ., Nareenart Traisuwan, Watcharra Chintakovid, Tatpong Tulyananda, Aussanee Pichakum","doi":"10.12982/nlsc.2023.069","DOIUrl":"https://doi.org/10.12982/nlsc.2023.069","url":null,"abstract":"Rainy season is an obstruction for efficient off-season longan production in Thailand. In this experiment, the bloom performances of off-season 'Phuang Thong' longan as affected by microclimate in a private longan orchard at Ban Phaeo district, Samut Sakhon province was evaluated during the rainy season of 2020. This study focused on the reproductive development at four different times (plots) of potassium chlorate application: May, June, July, and August. Air temperature, relative humidity and light intensity at the canopy level were recorded. Rainfall data was obtained from the Meteorological Department of Thailand. The results showed that inflorescence length, female flower number, and sex ratio of female to male of the May plot were the highest compared to other plots, and it was associated with early rainfall occurrence during flower development and blooming time. The August plot had a low growing degree day and a short flowering period, which coincided with frequent rainfall during the flower development period. However, the pollen viability and female flower characters were unaffected by environment. In conclusion, flower induction in the early rainy season, especially in May, was an effective time for the production of off-season ‘Phuang Thong’ longan in Ban Phaeo district, Samut Sakhon province, Thailand. Keywords: Floral biology, Flower, Pollen, Microclimate, Heat unit","PeriodicalId":471315,"journal":{"name":"Natural and Life Sciences Communications","volume":"166 ","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136067387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative and Stability Study of Rice Bran Oil in Nanovesicle: Conventional Niosomes and pH-Sensitive Niosomes","authors":"Sureewan Duangjit, Chudanut Akarachinwanit, Warisada Sila-on, Sureewan Bumrungthai, Praneet Opanasopit, Tanasait Ngawhirunpat","doi":"10.12982/nlsc.2023.070","DOIUrl":"https://doi.org/10.12982/nlsc.2023.070","url":null,"abstract":"Niosomes are more stable and economical than liposomes. In previous studies, conventional niosomes have shown advantages for transdermal delivery of bioactive compounds from rice bran. The objective of this study was to compare nanovesicle formulations (conventional niosomes and pH-sensitive niosomes) containing oryzanol (OZ) and rice bran oil (RBO). The formulation parameters were varied for the types of model drugs (OZ pure compound and RBO), the content of RBO loading (low, medium, high), and the types of niosomes (conventional niosomes and pH-sensitive niosomes). The physicochemical properties, e.g., vesicle size, size distribution, zeta potential and oryzanol content, were investigated. The stability of nanovesicles during the incubation period from Day 1 to Day 90 was observed. The in vitro skin permeation of the niosome formulation was demonstrated and calculated from the skin permeation flux and the permeability coefficient. The results indicated that the OZ- and RBO-loaded nanovesicle formulation had nanoscale vesicles (under 205 nm) and a narrow size distribution (0.22-0.55). The OZ content in the formulation was up to 300 µg/mL. The RBO-loaded nanovesicle formulations were stable at 4 and 25 °C for 90 days and 40 °C for 30 days. The skin permeation parameters of pH-sensitive niosomes were significantly higher than those of conventional niosomes at a flux of 16.56 ± 3.37 µg/cm2/h and a permeability coefficient of 0.0542 ± 0.011 cm2/h. These results suggest that pH-sensitive niosomes can be promoted and used as drug delivery carriers for rice bran oil. Keywords: Floral biology, Flower, Pollen, Microclimate, Heat unit Rice bran, Oryzanol, Liposome, Oleic acid, Permeability coefficient","PeriodicalId":471315,"journal":{"name":"Natural and Life Sciences Communications","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136067896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anticandidal Activity of Cajuput and Lemongrass Essential Oils Supplemented in Alcohol-Free Mouthwash Against Candida albicans Biofilm Formation on 96-Well Plate and Acrylic Surfaces","authors":"Rarinthorn Harintharanon, Chintana Itthidecharon, Phenphichar Wanachantararak, Siriwoot Sookkhee","doi":"10.12982/nlsc.2023.067","DOIUrl":"https://doi.org/10.12982/nlsc.2023.067","url":null,"abstract":"The present study aimed to evaluate the efficacy of alcohol-free mouthwash containing cajuput and lemongrass essential oils and their synergistic effect on eliminating Candida albicans biofilm formation, the most common causative agent of denture stomatitis. The inhibitory activity against C. albicans ATCC10231 biofilm formation on 96-well plate and acrylic surfaces of this formula was significantly different from 0.12% chlorhexidine solution at 20 minutes (P < 0.0001). At one hour and eight hours of immersion, the activity of this formula was similar to the activity of 0.12% chlorhexidine mouthwash. After treatment with this formula, there were less densely active cells and biofilm compared to the negative control, and the action was close to that of 0.12% chlorhexidine mouthwash. The minimal inhibitory concentrations of cajuput and lemongrass essential oils were 2 and 4 µL/mL (for 0.2% and 0.4% v/v, respectively). For chequerboard assays, the fractional inhibitory concentrations of these oils were 0.5 and 0.25 µL/mL, respectively. The combination of cajuput and lemongrass oils in this formula exhibited partial synergism against C. albicans ATCC10231 biofilm formation with a fractional inhibitory concentration index of 0.75. This study demonstrated the inhibitory activity of this formula against C. albicans biofilm formation on 96-well plate and acrylic surfaces after quantitated by colony enumeration and the XTT reduction assay resembled 0.12% chlorhexidine mouthwash. In conclusion, that C. albicans could be inhibited by the partial synergism of these essential oils in this mouthwash formula. Keywords: Inhibitory activity, Natural essential oils, Candida albicans, Microbial biofilm, Solid surface","PeriodicalId":471315,"journal":{"name":"Natural and Life Sciences Communications","volume":"75 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136295789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of Critical Limit of Zinc for Lentil (Lens culinaris L.) in Diverse Soils under Intensive Cropping Systems","authors":"Mousumi Akter, Khairul Alam, Harunur Rashid, Habib Mahammad Naser, Sarmin Sultana, Suman Biswas, Sohela Akhter, Ashraf Hossain, Akbar Hossain, Sharif Ahmed","doi":"10.12982/nlsc.2023.066","DOIUrl":"https://doi.org/10.12982/nlsc.2023.066","url":null,"abstract":"Determination of the critical limit (CL) for nutritionally essential zinc (Zn) is very important in developing fertilizer recommendations for crops and soils. However, the CL of Zn for the lentil crop has not been determined yet in Bangladesh, leading to the use of a fertilizer rate for the crop estimated on the basis of old critical limit generalised for all crops. A study was conducted in the Greenhouse facilities of the Soil Science Division of BARI, Gazipur, from October to April (Rabi season) of 2018/2019 and 2019/2020, to determine Zinc CL for soils and lentil crop in soils collected at twenty locations encompassing the six Agro-Ecological Zones (AEZs) of Bangladesh. For extraction of available Zn concentrations from soils and lentil biomass, a diethylene triamine penta acetic acid (DTPA) (0.005 M) solution was used. The DTPA-extractable Zn (mg kg-1) in the soils were from 0.43 to 4.10 and from 0.52 to 4.20 in 2018/2019 and 2019/2020, respectively. In all seasons, accessible Zn had negative but substantial correlations with soil pH, soil P, Ca, and Mg in available forms, but the association between available Zn and lentil dry matter was positive and significant (DM). Soil Zn had also a positive significant correlation with Zn concentration of lentil tissue Zn (0.501**). In 2018-2019 and 2019-2020, the CL of EDTA-extractable Zn was determined to be 0.75 mg kg-1 and 0.68 mg kg-1 in soils for lentil crop (0.72 mg kg-1 on an average), respectively, while it was 48.6 mg kg-1 for lentil tissue. The revised CL can be used for fertilizer recommendation for the lentil crop in the entire Gangetic Plains and other Agro-Ecological Zones of Bangladesh. Keywords: Pulse crop, Nutrient management, Micronutrient, Dryland crop, Sustainable agriculture","PeriodicalId":471315,"journal":{"name":"Natural and Life Sciences Communications","volume":"64 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135719394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gold Nanoparticles for Enhanced Skin Permeation of a Protein Drug","authors":"Kanokwan Singpanna, Chaiyakarn Pornpitchanarong, Prasopchai Patrojanasophon, Theerasak Rojanarata, Tanasait Ngawhirunpat, Boonnada Pamornpathomkul, Praneet Opanasopit","doi":"10.12982/nlsc.2023.065","DOIUrl":"https://doi.org/10.12982/nlsc.2023.065","url":null,"abstract":"Abstract This study aimed to evaluate the effect of gold nanoparticles (AuNPs) stabilized by citrate ions (Ci-AuNPs) and chitosan (CS-AuNPs) on skin permeation of a protein drug, albumin-fluorescein isothiocyanate conjugate (FITC-BSA). Ci-AuNPs and CS-AuNPs were prepared by the Turkevich method and microwave-assisted method, respectively. The particle size, surface charge, and morphology of the AuNPs were investigated. The skin permeation study through the porcine skin, skin permeation pathway, and the safety of the AuNPs were examined using vertical Franz diffusion cells, confocal laser scanning microscopy (CLSM), and MTT assay, respectively. AuNPs had a spherical shape with size ranging in a nano-scale (<100 nm). Ci-AuNPs exhibited negatively charged surfaces, while CS-AuNPs were positive. A co-delivery of FITC-BSA with CS-AuNPs showed approximately 3.5-fold greater permeability than the FITC-BSA alone. Interestingly, no significant improvement was observed from Ci-AuNPs co-delivery. The CLSM visualization suggested that the delivery of the model protein was enhanced through the transepidermal pathway. Furthermore, the CS-AuNPs presented no significant cytotoxicity toward normal human fibroblasts. Above all, the developed CS-AuNPs were proposed to be safe and effective nanosystem as a skin permeation enhancer for the transdermal delivery of proteins. Keywords: Gold nanoparticles, Chitosan, Skin permeation enhancer, Protein, Transdermal delivery","PeriodicalId":471315,"journal":{"name":"Natural and Life Sciences Communications","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135826158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Formulation Development and Evaluation of Chewable Gels Containing Jatuphalathika Extract","authors":"Aurasorn Saraphanchotiwitthaya, Natthapat  Saibuathong, Thanaphon  Boonsirirungrueng, Pattana Sripalakit","doi":"10.12982/nlsc.2023.060","DOIUrl":"https://doi.org/10.12982/nlsc.2023.060","url":null,"abstract":"Jatuphalathika (JP) is composed of the fruits of four herbal plants, Terminalia chebula Retz., Terminalia bellirica Roxb., Terminalia arjuna Wight and Arn., and Phyllanthus emblica L. JP formula is traditionally used for fever, as a carminative, for detoxification, and as an anti-hyperlipidaemic. However, consumption of JP is restricted due to its unpleasant flavours, which include sour, bitter, and astringent notes. The purpose of the current study was to formulate chewable gels with JP extract and evaluate their stability. Different types and concentrations of gelling agents and flavouring agents were used in the formulation. The finished products without or with a package (foil-wrapped or vacuum-sealed) were stored at room temperature (30 ± 2°C), in a refrigerator (2–8 °C) for 1 month, and under thermal cycling between 2–8 °C for 48 h and 45 °C for 48 h for 4 cycles. Stability testing of the chewable gels was carried out. Among six formulations, JP chewable gel containing gelatin, stevioside syrup, sorbitol solution, and paraben concentrate without (F5) or with menthol (F6) gave the best results. Both formulations maintained the same texture when stored in vacuum-sealed packaging: F5 at room temperature or in the refrigerator, and F6 at room temperature. Under these storage conditions, they were physically, chemically, and microbiologically stable with acceptable taste and scent. It may be concluded that chewable gels F5 and F6 are easy-to-use formulations which could improve patient or customer compliance when using JP. Keywords: Gallic acid, Gelatin, Obesity, Phytoconstituents, Stability, Stevia","PeriodicalId":471315,"journal":{"name":"Natural and Life Sciences Communications","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136064121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}