{"title":"Student identities predict classmate and instructor fear of negative evaluation among undergraduates in large-enrollment introductory biology courses.","authors":"Katelyn M Cooper, Carly A Busch, Sara E Brownell","doi":"10.1128/jmbe.00191-24","DOIUrl":"10.1128/jmbe.00191-24","url":null,"abstract":"<p><p>Undergraduate biology students describe fear of negative evaluation (FNE), defined as a sense of dread associated with being unfavorably evaluated in a social situation, as negatively affecting their experiences in active learning courses. Yet, few studies have examined who is prone to experiencing FNE, the severity and duration of FNE, and whether the effects of FNE are experienced equally among undergraduates. To address these gaps, we surveyed 494 undergraduates enrolled in active learning introductory biology courses about their FNE as it relates to students in their courses (classmate FNE) and to their instructor (instructor FNE). Students reported higher instructor FNE than classmate FNE. Using linear regressions, we found that women and nonbinary students, persons excluded based on ethnicity or race (PEERs), continuing-generation college students, LGBTQ+ students, and non-native English speakers had higher instructor FNE than their respective counterparts. PEER students also had higher classmate FNE than white students. Women, nonbinary, and PEER students were more likely than men and white students to report a greater negative impact of FNE on their abilities to articulate their thoughts about science in class. This work highlights the importance of reducing FNE with the intent to create more equitable active learning biology classrooms.</p>","PeriodicalId":46416,"journal":{"name":"Journal of Microbiology & Biology Education","volume":" ","pages":"e0019124"},"PeriodicalIF":1.5,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12369367/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144040317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Laboratory course development and implementation fostering teaching assistant growth.","authors":"R Telfah, A Roser, R Masoud, J Shultz","doi":"10.1128/jmbe.00197-24","DOIUrl":"10.1128/jmbe.00197-24","url":null,"abstract":"<p><p>STEM teaching assistants (TAs) are vital resources at the university level and are responsible for teaching most undergraduate laboratory courses. TAs are expected to navigate the complex interaction of personal confidence, course goals, varied student preparedness, and instructor supervision while delivering a quality educational experience. These variables combine to create an exceptionally prepared professional, a quick exit from the STEM field, or a capable individual in need of additional training. We present three individual TA development examples spread across three lab courses (Gel Electrophoresis, Genetics, and PCR Methods). Examples of these course materials are included, including links to the instructional resources for each of the three classes.</p>","PeriodicalId":46416,"journal":{"name":"Journal of Microbiology & Biology Education","volume":" ","pages":"e0019724"},"PeriodicalIF":1.5,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12369338/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144024404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Wendy Aquino Nunez, Meadow C B Scott, Dyan E Morgan
{"title":"Metacognitive mapping: developmental biology students struggle with higher-order cognitive skills in multiple course formats.","authors":"Wendy Aquino Nunez, Meadow C B Scott, Dyan E Morgan","doi":"10.1128/jmbe.00139-24","DOIUrl":"10.1128/jmbe.00139-24","url":null,"abstract":"<p><p>Reflective assignments are a powerful tool in undergraduate STEM courses to promote student metacognition and give instructors and researchers a view of that process. We were interested in understanding student challenges in our developmental biology course, so we used reflective assignments to uncover these challenges and also add to our understanding of student metacognition in upper-level courses. We used structural and initial coding and themed student responses. Then, we were able to compare results across two different course formats. We also compared these analyses with student performance on weekly formative assessments and completed item analysis. Our analyses suggest that students struggle the most with application. Notably, student responses did not differ in an asynchronous online class versus a high-flex in-person class. We also surveyed students (<i>n</i> = 162) on their use and perceived value of the metacognitive assignments for further comparison to other studies of upper-level student metacognitive regulation. Results indicate a strong majority of students completed more than half of the assignments, found the assignments beneficial to their learning, and used the opportunity to plan study time. Overall, we find that upper-level college students have accurate metacognitive knowledge, allowing them to identify challenging topics and cognitive skills, and have acquired the metacognitive regulation tools to develop study plans when prompted by a reflective assignment.</p>","PeriodicalId":46416,"journal":{"name":"Journal of Microbiology & Biology Education","volume":" ","pages":"e0013924"},"PeriodicalIF":1.5,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12369316/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144019963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Addison R Lane, Jennifer L Momsen, Lisa B Wiltbank-Chau
{"title":"Positive microbiology: addressing students' knowledge gaps regarding the benefits of microorganisms.","authors":"Addison R Lane, Jennifer L Momsen, Lisa B Wiltbank-Chau","doi":"10.1128/jmbe.00045-25","DOIUrl":"10.1128/jmbe.00045-25","url":null,"abstract":"<p><p>The associations of microbes with their environment can result in both positive and negative effects on humans. While most people are aware of the existence of microbes, research shows that perceptions of microbes and their effects are often incomplete or include misconceptions. Much like the positive psychology movement, the recently proposed \"positive microbiology\" movement can help society to see microbes as tools to benefit mankind. The purpose of this study was to examine college students' knowledge and perception of the positive effects of microbes (\"good\" microbes) and the negative effects of microbes (\"bad\" microbes). College students in the United States (<i>n</i> = 924) were surveyed about their perceptions of the characteristics, effects, and responses to bacteria and viruses. Students viewed viruses more negatively than bacteria. For both bacteria and viruses, students struggled to provide examples of beneficial effects of microbes. Thus, we see the need for more instruction about the benefits of microbes. To this end, we offer practical suggestions for this instruction, including research about best practices for teaching and suggested plug-and-play resources available for instructors to incorporate the topic of positive microbiology into their courses.</p>","PeriodicalId":46416,"journal":{"name":"Journal of Microbiology & Biology Education","volume":" ","pages":"e0004525"},"PeriodicalIF":1.5,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12369340/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144754803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Louise E O Darling, Sarah Pociask, John W Goss, Julie A Roden
{"title":"Increasing inquiry and collaboration: a faculty and undergraduate curricular intern partnership to redesign a cell biology laboratory course.","authors":"Louise E O Darling, Sarah Pociask, John W Goss, Julie A Roden","doi":"10.1128/jmbe.00190-24","DOIUrl":"10.1128/jmbe.00190-24","url":null,"abstract":"<p><p>Recent trends in undergraduate biology education include incorporating more inquiry/research-focused experiments into laboratory courses as a way to engage students and promote persistence in science. However, many faculty members face constraints in redesigning curricula, including a lack of time for course development. In addition, some undergraduate students find it difficult or intimidating to identify a first independent research position. We describe our experience working with student curricular interns to redesign a cell biology laboratory to be more inquiry-based. Students enrolled in the redesigned course felt that their course research experience was authentic and self-reported improvement in many fundamental scientific skills. Moreover, participating in the redesign project had positive effects on our curricular interns' experiences by exposing them to independent research, pedagogical design, and near-peer teaching. Thus, we believe that developing ways to introduce inquiry-based learning into curricula as a collaborative effort with undergraduate curricular interns has benefits for enrolled students, curricular interns, and faculty. We encourage other undergraduate science faculty to consider this model as they revise their courses.</p>","PeriodicalId":46416,"journal":{"name":"Journal of Microbiology & Biology Education","volume":" ","pages":"e0019024"},"PeriodicalIF":1.5,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12369356/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144024208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A freely available gel electrophoresis laboratory manual and course materials.","authors":"R Telfah, K Patrick, J L Shultz","doi":"10.1128/jmbe.00021-25","DOIUrl":"10.1128/jmbe.00021-25","url":null,"abstract":"<p><p>A laboratory manual and supporting material have been developed that use 14 different problems to illustrate basic gel electrophoresis concepts and to reinforce the scientific method. This material is appropriate for those interested in science in general or biology in particular and was specifically designed for implementation at the second-year level at a 4-year university or at a technical college. In order to maximize reliability, all labs use an inexpensive size standard, and most use a simple gel preparation protocol. This course has a low consumables cost per student, per term of approximately $20. A PDF of the complete manual and instructor materials is available from the author. Inexpensive, bound color lab manuals (standard and large print) are also available. Instructor materials include a detailed weekly schedule, 31 PowerPoint slides, answers to lab questions, and an assessment bank of 86 questions.</p>","PeriodicalId":46416,"journal":{"name":"Journal of Microbiology & Biology Education","volume":" ","pages":"e0002125"},"PeriodicalIF":1.5,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12369364/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144045842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Engaging social-emotional motivators to improve the grant writing experience: a workshop model for teaching scientific writing.","authors":"Courtney Peña, Miroslav Suzara, Crystal Botham","doi":"10.1128/jmbe.00065-25","DOIUrl":"10.1128/jmbe.00065-25","url":null,"abstract":"<p><p>Grant writing is an important skill that scientists must acquire in order to have successful careers as independent researchers. However, despite its importance, many scientists report that they do not enjoy grant writing because it is stressful, lonely, requires a lot of time and effort, and involves the possibility of rejection. As a result, many scientists have more negative associations with grant writing than positive ones. In this article, we offer a novel intervention in the form of a social-emotional learning (SEL)-informed workshop and accompanying handout for emerging scientists to build more positive associations with their writing. The approaches in this workshop intentionally leverage SEL motivators (such as scientific identity, sense of purpose, and community) to help grant writers overcome common challenges that accompany the writing process. A pre-post survey analysis of this workshop showed a shift from negative/challenge-focused attitudes and perceptions toward grant writing toward positive/process-focused ones after the workshop. This 1-hour intervention can be facilitated by research development professionals, lab leaders, or anyone teaching scientific writing for undergraduates, graduate students, and postdocs.</p>","PeriodicalId":46416,"journal":{"name":"Journal of Microbiology & Biology Education","volume":" ","pages":"e0006525"},"PeriodicalIF":1.5,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12369342/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144217239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Marissa Howard, Elyse Bolterstein, Kathryn P Kohl, Eric P Stoffregen, Christina I Swanson
{"title":"An interdisciplinary, collaborative CURE to investigate mutagen-sensitive alleles in <i>Drosophila melanogaster</i>.","authors":"Marissa Howard, Elyse Bolterstein, Kathryn P Kohl, Eric P Stoffregen, Christina I Swanson","doi":"10.1128/jmbe.00068-25","DOIUrl":"10.1128/jmbe.00068-25","url":null,"abstract":"<p><p>Course-based undergraduate research experiences (CUREs) are an effective strategy to teach students about the process of scientific research. Students participating in a CURE also benefit in numerous other ways, such as gaining increased enthusiasm for science and increased retention in a STEM field. Because scientific research is increasingly interdisciplinary and collaborative, recently developed CUREs have begun to incorporate interdisciplinary approaches and networking components. Interdisciplinary, networked CUREs have, in several cases, expanded learning gains for participating students beyond the benefits of traditional CUREs. Here, we describe a novel interdisciplinary, collaborative CURE. Our CURE was launched with four participating primarily undergraduate institutions (PUIs) and the broad research goal of characterizing mutagen-sensitive alleles in <i>Drosophila melanogaster</i>. Student surveys suggest that our CURE has successfully contributed to learning gains across a variety of areas, including increased project ownership, increased experience with collaboration, and increased experience with interdisciplinary research. Furthermore, participating faculty have benefitted from increased scholarly productivity. Our CURE could be expanded to include additional institutions, or could serve as a model for PUI faculty interested in developing their own collaborations as a strategy to tackle broad, interdisciplinary projects.</p>","PeriodicalId":46416,"journal":{"name":"Journal of Microbiology & Biology Education","volume":" ","pages":"e0006825"},"PeriodicalIF":1.5,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12369339/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144477169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kevin Hernández García, Alexander H Bower, Ana Elisa García-Vedrenne
{"title":"Mentoring the mentors: a scalable professional development model for graduate teaching assistants and undergraduate learning assistants in large-enrollment CUREs.","authors":"Kevin Hernández García, Alexander H Bower, Ana Elisa García-Vedrenne","doi":"10.1128/jmbe.00040-25","DOIUrl":"10.1128/jmbe.00040-25","url":null,"abstract":"<p><p>Course-based undergraduate research experiences (CUREs) incorporate authentic research into undergraduate curricula, offering significant benefits in student retention, graduation rates, and pursuit of science, technology, engineering, and mathematics (STEM) careers. However, graduate teaching assistants (GTAs) and undergraduate learning assistants (ULAs) rarely receive the specific pedagogical training required for CURE instruction. We share an integrated professional development (PD) model for GTAs and ULAs in a large-enrollment CURE that addresses this need. Our model integrates training for both GTAs and ULAs simultaneously into mandatory instructional meetings, providing just-in-time training for upcoming topics and addressing unique CURE facilitation needs. This PD model has been effective in a CURE with a large instructional team and can be scaled up or down as needed. We present results from a post-pre survey asking instructional team members to reflect on their self-efficacy regarding the nine learning outcomes of the implementation. All learning outcomes show an increase in self-efficacy for GTAs and ULAs. By equipping both GTAs and ULAs to navigate the unique challenges of CURE instruction, this PD model may enhance the overall effectiveness of these valuable undergraduate research experiences.</p>","PeriodicalId":46416,"journal":{"name":"Journal of Microbiology & Biology Education","volume":" ","pages":"e0004025"},"PeriodicalIF":1.5,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12369363/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144530324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A 3D-printed Sanger sequencing activity addresses student misconceptions.","authors":"Phillip E Brown, Stefanie H Chen","doi":"10.1128/jmbe.00209-24","DOIUrl":"10.1128/jmbe.00209-24","url":null,"abstract":"<p><p>The process of Sanger sequencing can be a challenging and unintuitive concept for students to master. In order to improve student learning, we developed a hands-on Sanger sequencing activity using 3D-printed models to incorporate tactile learning. These 3D models and the accompanying activity demonstrate the differences between gene amplification polymerase chain reaction (PCR) and Sanger sequencing, including the purpose and function of dNTPs and ddNTPs, both in terms of building and terminating the chain and in how the DNA sequence is read. After completing the activity, students self-reported high levels of both learning and enjoyment from the activity. Students were also asked to discuss what misconceptions they had prior to this activity that were addressed and provide suggestions for improving this activity. A majority of the misconceptions are related to the function and differences between dNTPs and ddNTPs, with others related to the function of primers, the high-quality region of sequencing, and the purpose of DNA fragment sizes. Overall, student responses indicate that this activity was enjoyable, improved student learning, and addressed specific misconceptions regarding Sanger sequencing. The use of online dice rolling software or additional computational analysis was a common suggestion from students to improve this activity further in future semesters.</p>","PeriodicalId":46416,"journal":{"name":"Journal of Microbiology & Biology Education","volume":" ","pages":"e0020924"},"PeriodicalIF":1.5,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12369333/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144053730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}