Rohini Ganjoo, James Rankin, Benjamin Lee, Lisa Schwartz
{"title":"Beyond boundaries: exploring a generative artificial intelligence assignment in graduate, online science courses.","authors":"Rohini Ganjoo, James Rankin, Benjamin Lee, Lisa Schwartz","doi":"10.1128/jmbe.00127-24","DOIUrl":"10.1128/jmbe.00127-24","url":null,"abstract":"<p><p>Generative artificial intelligence (GAI) offers increased accessibility and personalized learning, though the potential for inaccuracies, biases, and unethical use is concerning. We present a newly developed research paper assignment that required students to utilize GAI. The assignment was implemented within three online, asynchronous graduate courses for medical laboratory sciences. Student learning was assessed using a rubric, which rated students' effective integration and evaluation of GAI-generated content against peer-reviewed research articles, thus demonstrating their critical thinking and synthesis skills, among other metrics. Overall rubric scores were high, suggesting that learning outcomes were met. After field testing, we administered a 16-item survey about GAI utilization, contribution to learning, and ethical concerns. Data (<i>n</i> = 32) were analyzed, and free-response answers were thematically coded. While 93.8% of respondents found the GAI-generated content to be \"very good\" or \"excellent,\" 28.1% found inaccuracies, and 68.8% \"strongly agreed\" or \"agreed\" that GAI should be allowed to be used as a tool to complete academic assignments. Interestingly, however, only 28.1% \"strongly agreed\" or \"agreed\" that GAI may be used for assignments if not explicitly authorized by the instructor. Though GAI allowed for more efficient completion of the project and better understanding of the topic, students noted concerns about academic integrity and the lack of citations in GAI responses. The assignment can easily be modified for different learning preferences and course environments. Raising awareness among students and faculty about the ethical use and limitations of GAI is crucial in today's evolving pedagogical landscape.</p>","PeriodicalId":46416,"journal":{"name":"Journal of Microbiology & Biology Education","volume":" ","pages":"e0012724"},"PeriodicalIF":1.6,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11636326/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142569818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Development of a simple, low-cost, blue light-emitting diode illuminator for hands-on training of DNA detection experiments using agarose gel electrophoresis.","authors":"Haruka Honda, Hisayuki Miyajima, Noritaka Miyamoto, Keigo Yoshida, Yuichi Tanaka","doi":"10.1128/jmbe.00083-24","DOIUrl":"10.1128/jmbe.00083-24","url":null,"abstract":"<p><p>DNA detection by agarose gel electrophoresis (AGE) is commonly used in molecular biology. AGE is a separation method that provides opportunities for students to learn about the topology and size of DNA molecules. Recently, several fluorescent dyes have been used for DNA staining owing to their convenience, safety, reduced toxicity, and high sensitivity. A blue light-emitting diode (LED) transilluminator is required to detect DNA using fluorescent dyes; however, the associated high cost may limit its availability in classrooms or small laboratories. Therefore, we have designed a simple, low-cost blue LED illuminator to enable easy assembly for instructors and students. We evaluated the performance of the proposed illuminator by observing fluorescent dye-stained DNA markers using AGE, revealing clear DNA marker bands. Despite its limited functionality, the ease of construction and affordability of the proposed illuminator make it sufficient for hands-on molecular biology training in classrooms, thereby enhancing the learning environment and educational efficiency.</p>","PeriodicalId":46416,"journal":{"name":"Journal of Microbiology & Biology Education","volume":" ","pages":"e0008324"},"PeriodicalIF":1.6,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11636322/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142630215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Advancing research ethics instruction in laboratory courses.","authors":"Joseph Ross","doi":"10.1128/jmbe.00106-24","DOIUrl":"10.1128/jmbe.00106-24","url":null,"abstract":"","PeriodicalId":46416,"journal":{"name":"Journal of Microbiology & Biology Education","volume":" ","pages":"e0010624"},"PeriodicalIF":1.6,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11636084/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142000931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Optogenetic control of <i>Drosophila</i> neurons: a laboratory practical for undergraduates and outreach.","authors":"Andrew D Renault","doi":"10.1128/jmbe.00086-24","DOIUrl":"10.1128/jmbe.00086-24","url":null,"abstract":"<p><p>Teaching aspects of neuroscience to large undergraduate classes can be difficult in terms of the cost of equipment involved such as microscopes and electrophysiology equipment, the time taken to master techniques such as dissection or intracellular recording, and ethical concerns when using vertebrates. Here, I describe a practical that uses behavioral readouts and optogenetics on <i>Drosophila</i> that can be implemented with minimal cost as well as reduced ethical concerns and uses mostly observational techniques. The practical can be used to teach aspects of genetics and the tools for manipulating neuronal activity for ascribing neuronal function. The practical can be customized to fit different undergraduate levels and learning objectives.</p>","PeriodicalId":46416,"journal":{"name":"Journal of Microbiology & Biology Education","volume":" ","pages":"e0008624"},"PeriodicalIF":1.6,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11636384/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142120865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nancy Boury, Amy Siegesmund, David B Kushner, Davida S Smyth, Mary E Allen, Adronisha Frazier, Illona Gillette-Ferguson, Miriam Markum, Glenn Patriquin, Sara E Reynolds, Sarah Rosario, J Jordan Steel, Rachel Horak
{"title":"Updated ASM Curriculum Guidelines describe core microbiology content to modernize the framework for microbiology education.","authors":"Nancy Boury, Amy Siegesmund, David B Kushner, Davida S Smyth, Mary E Allen, Adronisha Frazier, Illona Gillette-Ferguson, Miriam Markum, Glenn Patriquin, Sara E Reynolds, Sarah Rosario, J Jordan Steel, Rachel Horak","doi":"10.1128/jmbe.00126-24","DOIUrl":"10.1128/jmbe.00126-24","url":null,"abstract":"<p><p>Curricular guidelines promote standardized approaches to coverage of essential knowledge and skills in undergraduate education. The American Society for Microbiology (ASM) Curriculum Guidelines for Undergraduate Microbiology were developed in 2012. Continuous, rapid growth of knowledge in science and a dynamic, changing world necessitate updates to these guidelines. As such, ASM formed a task force in the summer of 2022. The task force assessed the 2012 ASM Curriculum Guidelines considering advancements in technology, an understanding of an expanded role of microbes, and a broader scope addressing relevant social and environmental aspects of microbiology. Language in the updated guidelines was also modified to better include eukaryotic microbes, viruses, and other acellular microbes. The task force formed working groups, each aimed at revising specific sections of the 2012 ASM Curriculum Guidelines. The revisions to the ASM Curriculum Guidelines were reviewed by subject matter experts and education stakeholders. Feedback from this peer review was incorporated into the updated guidelines, and further comments were solicited from the ASM Conference of Undergraduate Educators (ASMCUE) attendees in November 2023 before these guidelines were finalized. In this article, we describe the rationale and development of updated ASM Curriculum Guidelines which identify foundational concepts that will serve to improve microbial literacy and that can be expanded upon to address more advanced and specialized topics.</p>","PeriodicalId":46416,"journal":{"name":"Journal of Microbiology & Biology Education","volume":" ","pages":"e0012624"},"PeriodicalIF":1.6,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11636387/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142477382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Demystifying anaerobic respiration: a problem-solving exercise.","authors":"Tomas Linder","doi":"10.1128/jmbe.00044-24","DOIUrl":"10.1128/jmbe.00044-24","url":null,"abstract":"<p><p>Anaerobic respiration reactions are of fundamental importance to global biogeochemical cycling of elements. Yet, the idea that cellular respiration can occur not only in the absence of oxygen but also involve the oxidation of inorganic substrates (e.g., AsO<sub>3</sub><sup>3-</sup>, Fe<sup>2+</sup>, H<sub>2</sub>, H<sub>2</sub>S, Mn<sup>2+</sup>, NH<sub>3</sub>, and S<sup>0</sup>) is often foreign to many undergraduate students. This article describes a problem-solving exercise where students are introduced to the thermodynamic fundamentals of respiration with a particular focus on the role of redox (reduction-oxidation) potentials (<i>E<sub>0</sub>´</i>). In the exercise, the students investigate how the difference in redox potential (<i>ΔE<sub>0</sub>´</i>) between different pairs of reductants and oxidants affects the range of permissible microbial metabolic reactions in natural environments when oxygen is absent.</p>","PeriodicalId":46416,"journal":{"name":"Journal of Microbiology & Biology Education","volume":" ","pages":"e0004424"},"PeriodicalIF":1.6,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11636275/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142000932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Visualization of giant <i>Mimivirus</i> in a movie for biology classrooms.","authors":"Kanako Morioka, Ayumi Fujieda, Masaharu Takemura","doi":"10.1128/jmbe.00138-24","DOIUrl":"10.1128/jmbe.00138-24","url":null,"abstract":"<p><p>We have developed a new observation chamber for <i>Mimivirus</i>-infected <i>Acanthamoeba</i> to create dynamic visual teaching materials for virus education suitable for high school and university biology courses. We conducted experiments and captured a movie showcasing the infection process of <i>Acanthamoeba</i> cells by mimiviruses. In this educational film, we successfully recorded the active movement of healthy <i>Acanthamoeba</i> cells across the surface of a culture flask under an agarose gel. After <i>Mimivirus</i> infection, the movement of the <i>Acanthamoeba</i> cells gradually slowed and eventually stopped. This cessation coincided with the development of the <i>Mimivirus</i> virion factory, which began producing new virions on the surface of the host cells. Moreover, we captured continuous footage of a single cell throughout the viral proliferation process, thereby illustrating the viral proliferation in real time. This educational movie, which visually demonstrates the proliferation of <i>Mimivirus</i> within host cells, acts as an effective teaching tool. Moreover, it enhances students' understanding of virus proliferation mechanisms and highlights the biological significance of viruses, their impact on host cell fate, and their role in ecosystems.</p>","PeriodicalId":46416,"journal":{"name":"Journal of Microbiology & Biology Education","volume":" ","pages":"e0013824"},"PeriodicalIF":1.6,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11636218/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142606831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Zotero as a teaching tool for independent study courses, honors contracts, and undergraduate research mentoring.","authors":"Dyan E Morgan","doi":"10.1128/jmbe.00132-24","DOIUrl":"10.1128/jmbe.00132-24","url":null,"abstract":"<p><p>Managing and evaluating the literature review process can be a time-consuming challenge when working one-on-one with students to teach scientific process skills, whether through an independent study, honors contract, or undergraduate research. In this article, I share my solution to address the pedagogical and organizational challenge posed by this type of teaching and mentoring: the citation management software Zotero used in conjunction with a template worksheet. Overall, this approach will save time, introduce students to a new multipurpose software tool, and lead to a set of reusable resources for future teaching.</p>","PeriodicalId":46416,"journal":{"name":"Journal of Microbiology & Biology Education","volume":" ","pages":"e0013224"},"PeriodicalIF":1.6,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11636089/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142000933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ashley L Waring-Sparks, Rachel A Waring-Sparks, Rebekka Darner, Nathan T Mortimer
{"title":"Advanced biology students' individual conceptions of scientific researchers after participating in biomedically relevant CRE.","authors":"Ashley L Waring-Sparks, Rachel A Waring-Sparks, Rebekka Darner, Nathan T Mortimer","doi":"10.1128/jmbe.00183-23","DOIUrl":"10.1128/jmbe.00183-23","url":null,"abstract":"<p><p>While undergraduate research has been shown to be a high-impact educational practice, it is logistically impossible for all undergraduate biology majors to have long-term faculty-mentored research experience. Therefore, biology educators and researchers must devise opportunities to engage more students in undergraduate research outside of working directly in their labs. Course-Based Research Experiences (CREs), structured as authentic research experiences, are one such opportunity. In this work, we describe the effects of a CRE with biomedical relevance on students' research skills, attitudes toward science, and perceptions of scientific research and scientific researchers. Results demonstrate that students gained experience in independent research skills including designing their own research project, being accountable for part of a project, and writing a research proposal. Students' perceptions of scientific research and researchers, assessed by the Draw-A-Researcher Task, did not show changes among the whole group, but individual analysis yielded meaningful results related to students' personal changes in how they perceived research and researchers, including their perception of themselves as researchers. This work demonstrates the substantial impact of CREs on upper-level biology undergraduate and graduate students.</p>","PeriodicalId":46416,"journal":{"name":"Journal of Microbiology & Biology Education","volume":" ","pages":"e0018323"},"PeriodicalIF":1.6,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11636289/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142074194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Esteban Pazmiño-Arias, Abigail Montero-Calderon, Mariela Perez-Cárdenas, Marco Esteban Gudiño Gomezjurado
{"title":"From local bacterial communities to energy: application of microbial fuel cells in laboratory experimentation.","authors":"Esteban Pazmiño-Arias, Abigail Montero-Calderon, Mariela Perez-Cárdenas, Marco Esteban Gudiño Gomezjurado","doi":"10.1128/jmbe.00133-24","DOIUrl":"10.1128/jmbe.00133-24","url":null,"abstract":"<p><p>It is well known that bacterial communities are an essential component to maintain the balance of terrestrial ecosystems due to the functions and services performed by microorganisms in the environment. The research seeking on alternative energy sources has shown that bacterial communities can bioconvert the chemical energy of an organic substrate into electrical energy, within devices known as microbial fuel cells. For this reason, this class project allows students of Biotechnology, Environmental Science, and Microbiology to apply the appropriate methodology to develop a class project throughout an environmental bacterial community capable of generating electrical energy.</p>","PeriodicalId":46416,"journal":{"name":"Journal of Microbiology & Biology Education","volume":" ","pages":"e0013324"},"PeriodicalIF":1.6,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11636364/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142074195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}