Clinical Plasma Medicine最新文献

{"title":"OBC","authors":"","doi":"10.1016/S2212-8166(18)30009-X","DOIUrl":"https://doi.org/10.1016/S2212-8166(18)30009-X","url":null,"abstract":"","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":"9 ","pages":"Page OBC"},"PeriodicalIF":0.0,"publicationDate":"2018-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S2212-8166(18)30009-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"137433022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gwu-Usmi Plasma Medicine Research Program","authors":"Jerome Canady ,&nbsp;Barry Trink ,&nbsp;Jonathan Sherman ,&nbsp;Michael Keidar","doi":"10.1016/j.cpme.2017.12.004","DOIUrl":"10.1016/j.cpme.2017.12.004","url":null,"abstract":"<div><p><span>This talk will introduce the recently established joint program on plasma cancer therapy between USMI and GWU. The use of cold atmospheric plasma-based instruments resulting from prior collaborations between USMI and GWU have already shown promise in the treatment of cancer. In January 2016, a surgical team used the Canady Helios</span>^TM<span><span> Cold Plasma Scalpel to selectively kill cancerous tissue during a two-stage liver resection in a patient with advanced inoperable liver cancer. The innovative technology allowed the surgical removal of the cancerous tissue without damaging the </span>blood supply<span> to the remaining liver. This research program will enable the transition of the novel CAP cancer treatment technology into translational and clinical stages by the development of new self-adaptive plasma devices, development of in-situ diagnostics to probe cell and tissue response to plasma action including optical, magnetic and thermal imaging, and the development of remote (non-surgical) techniques for plasma treatment.</span></span></p></div>","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":"9 ","pages":"Page 3"},"PeriodicalIF":0.0,"publicationDate":"2018-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cpme.2017.12.004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80935119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanosecond-Pulsed DBD Plasma For A Clinical Trial Of Actinic Keratosis","authors":"Vandana Miller MD ,&nbsp;Abraham Lin ,&nbsp;Gregory Fridman ,&nbsp;Alexander Fridman ,&nbsp;Peter Friedman MD","doi":"10.1016/j.cpme.2017.12.068","DOIUrl":"10.1016/j.cpme.2017.12.068","url":null,"abstract":"<div><p><span><span><span>Actinic keratosis<span> (AK) is a pre-cancerous skin lesion, the earliest clinical stage in a continuum of </span></span>malignancy<span><span> that may lead to squamous cell carcinoma (SCC), a skin cancer that accounts for significant morbidity (over 1 million cases in the US annually), and thousands of preventable deaths annually. This translates to a total direct cost for AK of about $1.2 billion and approximately 8.2 million office visits annually. Available treatments ablate lesions via </span>cryotherapy, </span></span>curettage<span><span> or long-term application of chemotherapeutic or immune-modulator drugs. They are typically associated with pain, scarring and inflammation, undesirable side effects that reduce patient compliance. To address these, we studied the use of non-equilibrium, atmospheric pressure plasma (NEAPP) for the treatment of AK in a small </span>clinical trial<span>. [1] One month post NEAPP treatment, over 50% of the lesions achieved full clinical resolution or showed significant improvement. Abscopal effects<span> were observed in some cases, possibly through immune stimulation. [2] Furthermore, no patient experienced adverse effects, immediate or delayed. One year follow-up showed no recurrence of any of the treated lesions. [unpublished data] 2-year follow-up results and further optimization of treatment under a new clinical trial will be presented. Our results demonstrate that NEAPP may serve as a potential, effective treatment for AKs.</span></span></span></span><span><figure><span><img><ol><li><span>Download : <span>Download high-res image (166KB)</span></span></li><li><span>Download : <span>Download full-size image</span></span></li></ol></span></figure></span></p><p>Evaluation of nspDBD treatment of AK. 9 lesions fully resolved, 3 showed significant improvement</p></div>","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":"9 ","pages":"Page 44"},"PeriodicalIF":0.0,"publicationDate":"2018-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cpme.2017.12.068","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77347629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AlmaIDEA Project: Chemo-Physical And Biological Mechanisms Behind The Antitumor Activity Of Plasma Activated Liquids For The Treatment Of Peritoneal Carcinosis From Primitive Epithelial Ovarian/Tubular Tumor","authors":"R. Laurita ,&nbsp;M. Gherardi ,&nbsp;A. Stancampiano ,&nbsp;A. Miserocchi ,&nbsp;A.M. Porcelli ,&nbsp;A.M. Perrone ,&nbsp;V. Colombo ,&nbsp;G. Gasparre ,&nbsp;P. De Iaco","doi":"10.1016/j.cpme.2017.12.062","DOIUrl":"10.1016/j.cpme.2017.12.062","url":null,"abstract":"<div><p><span><span>Advanced ephitelial ovarian cancer is characterized by the diffusion of the disease as nodules or plaques from the ovary to peritoneal surfaces (carcinosis) (Fig.1). In advanced stages the disease presents poor prognosis (OS Figo stage III-IV: 15-20% within 5 years) [1]. Nowadays, multimodal treatments, surgery and intravenous/intraperitoneal chemotherapy with platinum and taxanes, can increase the survival rate but not eradicate the disease and about 80% of patients relapse ), because chemotherapeutic agents show low efficacy against resistant tumor subclones. For this reason, there is a continuous need for novel therapies. Since the treatment of liquids by means of cold atmospheric pressure plasma enables the production of plasma activated liquids (PALs) containing reactive oxygen and nitrogen species (RONS) having </span>antitumor activity<span><span> [2-3], the direct application of PALs on peritoneal surfaces could represents an alternative treatment of carcinosis. In this context, the aim of this work is to present preliminary results on the effect of PALs on human ovarian </span>carcinoma cells </span></span><em>in vitro.</em> The research is performed in the frame of the Alma<em>IDEA</em><span><span><span> project focused on the investigation of PALs (plasma activated liquids) as potential antitumor agent in the treatment of peritoneal carcinosis (Fig.1). The composition of the team, that brings together surgeons of </span>gynecologic oncology, geneticists (with a bias on </span>mitochondrial metabolism in solid tumors) and plasma engineers, allows to have a multidisciplinary approach to the research, whose final aim is the development of a novel intraperitoneal therapy for cancer treatment, starting from the investigation of the bio-chemical effect of PALs on cells </span><em>in vitro</em>.<span><figure><span><img><ol><li><span>Download : <span>Download high-res image (361KB)</span></span></li><li><span>Download : <span>Download full-size image</span></span></li></ol></span></figure></span></p><p>Figure 1: Peritoneal carcinosis (red arrows) close to ovary (right) and PAL production using microsecond pulsed DBD jet (left).</p></div>","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":"9 ","pages":"Pages 39-40"},"PeriodicalIF":0.0,"publicationDate":"2018-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cpme.2017.12.062","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78336901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immune modulatory properties of radiotherapy","authors":"Udo S. Gaipl","doi":"10.1016/j.cpme.2017.12.020","DOIUrl":"10.1016/j.cpme.2017.12.020","url":null,"abstract":"<div><p><span><span>Radiotherapy (RT) is a common treatment for cancer and about 60% of all cancer patients will receive it during their course of illness. RT primarily aims to achieve local tumor control. The induction of DNA damage by </span>reactive oxygen species<span><span> (ROS), tumor cell death and the modulation of the </span>tumor microenvironment are the main effects of ionizing irradiation to reduce tumor masses, but also to modulate the immune system. RT might thereby act as an </span></span><em>in situ</em> cancer vaccine under certain microenvironmental conditions. However, RT also fosters the upregulation of immune suppressive molecules such as immune checkpoint molecules.</p><p><span><span>The presentation will focus on how local irradiation changes the tumor cell phenotype and the tumor microenvironment and consecutively does impact on local and systemic changes in immune cell compositions. In particular the impact of ROS, danger signals and cytokines on it will be outlined. The dynamics of immune changes, the </span>radiosensitivity of distinct immune cells as well as biological basis for reasonable combination of RT with </span>immune stimulation<span> will be discussed in detail, as well as how radiation-induced immune suppression can be overcome. Based on the pre-clinical knowledge, innovative clinical study concepts of radio-immune treatments will be presented.</span></p></div>","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":"9 ","pages":"Page 13"},"PeriodicalIF":0.0,"publicationDate":"2018-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cpme.2017.12.020","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77869931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Generation Of Reactive Species By Plasma Needle In Different Liquids","authors":"Inés Hamouda ,&nbsp;Alix Gouhier ,&nbsp;Cédric Labay ,&nbsp;Maria-Pau Ginebra ,&nbsp;Cristina Canal","doi":"10.1016/j.cpme.2017.12.054","DOIUrl":"10.1016/j.cpme.2017.12.054","url":null,"abstract":"<div><p>Plasma activated media (PAM) is produced by exposing liquids to cold atmospheric plasmas [1,2]. PAM has gained increasing attention due to its capacity to kill cancer cells<span> as effectively as direct treatment of cells in culture by cold plasmas.</span></p><p>In previous works [3] we could show the selectivity of an atmospheric pressure plasma needle on osteosarcoma cell lines versus healthy bone cells. The cytotoxicity of the direct plasma treatment on cells was comparable to treatment with PAM, wherein in this case the liquid selected was cell culture medium.</p><p>The rationale beyond employing PAM lies in being able to avoid the effects of electrical field, or UV/VIS radiation present in plasmas, and its biological effects seem to lay in the reactive oxygen species<span> (ROS) and reactive nitrogen species (RNS) generated in the aqueous state. The concentration of ROS and RNS in the PAM is directly related to its effectiveness in killing cancer cells. It is our interest to evaluate the different parameters influencing the generation of ROS and RNS.</span></p><p>In this work different liquid media (different cell culture media, aqueous solutions such as water or ringer’s saline, etc.) are compared, and the production of ROS and RNS is quantified in different conditions (volume of liquid, treatment times, distance to the nozzle). The stability of the mentioned species is evaluated with time.<span><figure><span><img><ol><li><span>Download : <span>Download high-res image (106KB)</span></span></li><li><span>Download : <span>Download full-size image</span></span></li></ol></span></figure></span></p><p>Figure 1. Production of H₂O₂ and NO₂^- by APPJ treatment on different cell culture medium (a) McCoy and (b) AdvDMEM.</p></div>","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":"9 ","pages":"Pages 34-35"},"PeriodicalIF":0.0,"publicationDate":"2018-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cpme.2017.12.054","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85083567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating Electron And Radical Interactions With Biomolecules And Cells Using A Droplet In Plasma Laboratory","authors":"Paul Maguire,&nbsp;Harold McQuaid,&nbsp;David Rutherford,&nbsp;Davide Mariotti","doi":"10.1016/j.cpme.2017.12.050","DOIUrl":"10.1016/j.cpme.2017.12.050","url":null,"abstract":"&lt;div&gt;&lt;p&gt;&lt;span&gt;Plasmas-liquid interactions are a valuable source of radical species for plasma medicine including cancer treatment and antimicrobial applications. However untangling the complex interplay between species and biology over very short timescales and distances is proving extremely challenging. Radiolysis induced damage to DNA is considered to be significantly affected by low energy secondary species such as OH radicals but also via direct interaction with low energy solvated electrons (LEE). Pt adducts and the presence of Au &lt;/span&gt;nanoparticles are also known to enhance the LEE effectiveness. However direct investigation of LEE and radical interactions with biomolecules e.g. DNA in a liquid environment at atmospheric pressure or in a living cell present very significant experimental challenges. LEE sources have been proposed such as 2D radioactive layers and UV-induced emission from metals. In this work we look at the potential of a living laboratory based on small liquid droplets containing biomolecules or cells passed through a plasma and exposed to a high flux of electrons and selectable radicals. We also consider the potential of using droplets to deliver plasma-activated media near instantaneously downstream.&lt;/p&gt;&lt;p&gt;Transport of micron-sized liquid droplets through a low temperature non-equilibrium RF plasma [1] at atmospheric pressure has demonstrated a number of remarkable and unexpected effects. The microdroplet system allows for a controlled (air-free) gas ambient environment, a large surface area to volume ratio, very small reaction volume and low droplet temperature. In addition, flow induced convection in the liquid can be minimised. After a very short plasma exposure time, ~120°μ s, there is evidence that chemical reactions induced by the plasma and gas flux proceed at a rate that is significantly faster that observed in plasma – bulk liquid studies and many orders of magnitude faster than in standard bulk chemistry [2]. The high chemical reactivity is thought to depend not only on the picolitre droplet volume, as in microreaction chemistry, but also on the high level of surface charge due to net electron bombardment in the plasma and the high flux of low energy electrons which arrive at the charged droplet with almost zero net energy. We have observed very rapid electron reduction of metal (Au) salts to form Au nanoparticles at rates that are much greater than observed with gamma radiolysis or high energy electron beams. This points to the possible effectiveness of plasma-generated Ultra-Low Energy Electrons (ULEE) in reduction reactions that may prove valuable for electron – biomolecule studies. We have also observed H&lt;sub&gt;2&lt;/sub&gt;O&lt;sub&gt;2&lt;/sub&gt; and OH in the liquid most likely due to generation of these species in the plasma phase, which contains only He and H&lt;sub&gt;2&lt;/sub&gt;O vapour. We have used droplets as carriers for single bacteria cells, which are then exposed to plasma species (electrons, OH and H&lt;sub&gt;2&lt;/sub&gt;O&lt;sub&gt;2&lt;/","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":"9 ","pages":"Pages 31-32"},"PeriodicalIF":0.0,"publicationDate":"2018-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cpme.2017.12.050","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76258351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of RAC1 Gene Expression Under Exposure Of Plasma Activated Verbascoside in HT-29 Colorectal Cancer Cells","authors":"Kobra Hajizadeh ,&nbsp;Bahram Behzad ,&nbsp;Danial Seifi ,&nbsp;Hassan Mehdian ,&nbsp;Mohammad Nabiouni ,&nbsp;Kamal Haji-Sharifi ,&nbsp;Elahe Amini","doi":"10.1016/j.cpme.2017.12.057","DOIUrl":"10.1016/j.cpme.2017.12.057","url":null,"abstract":"<div><p>Colon cancer is one of the most common and most prevalent Neoplasma in men; adenocarcinomas make up 95 percent of all colon cancer cases. Due to the side effects of conventional treatment methods such as chemotherapy, today, attentions have been attracted to natural compounds that have less side effects and anticancer properties.</p><p>This study aims to investigate the effect of Verbascoside as a natural compound in inhibiting HT-29 signaling pathway which is an effective route in the metastasis, angiogenesis and cell migration in the human cell line of HT-29 colon cancer; and also investigates the possibility of enhancing and raising the effect of this herbal drug by cold atmospheric pressure plasma jet.</p><p>A the first step, HT-29 cells were treated at different concentrations of verbascoside 10, 20, 30, 40, 50, 70, 100 μg / ml for 24 hours, then MTT test was used to calculate the inhibitory concentration. The colon cancer cell migration was evaluated by scratch test and HIF-1α gene expression was evaluated by Real Time PCR. The results of the MTT assay showed that verboscoside dose-dependent inhibition of cancer cells. The HT-29 colon is at a concentration of 50 μg / ml. Scratch repair tests showed that verboscoside reduced the migration of cells in a dose-dependent manner, and the results of Real Time PCR showed a decrease in the expression of HIF-1α gene expression. Cancer tumors undergo some changes in oxygen deficiency conditions, including increasing the expression of the HIF-1α gene, which in turn play an important role in activating and enhancing the expression of genes involved in metastasis, angiogenesis and migration.</p><p>At the second step of this study, verboscoside was primarily treated by cold atmospheric plasma jet then the same procedure has been taken for plasma activated verboscoside. In this study helium jet has been used.</p><p>The findings of this study showed that verboscosid with inhibitory expression of HIF-1α has a significant anti-metastatic, cellular angiogenesis and migration effect and may be a good option for reducing metastasis and angiogenesis in colon cancer models; it has been also showed that plasma activated verboscosid is more efficacious in all of the above mentioned features.</p></div>","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":"9 ","pages":"Pages 36-37"},"PeriodicalIF":0.0,"publicationDate":"2018-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cpme.2017.12.057","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89402893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Laser-Assisted Delivery Of Cold Atmospheric Plasma In Unresectable Cutaneous Metastasis In Melanoma Patients","authors":"Philipp Jansen ,&nbsp;Dirk Schadendorf ,&nbsp;Sander Bekeschus ,&nbsp;Joachim Klode ,&nbsp;Ingo Stoffels","doi":"10.1016/j.cpme.2017.12.071","DOIUrl":"10.1016/j.cpme.2017.12.071","url":null,"abstract":"<div><p>According to the World Health Organization, the incidence of melanoma<span><span> is increasing faster than that of any other major cancer in the world. Melanoma is the fifth most common cancer in the United States, posing a substantial health and economic burden. Treatment of early and multiple cutaneous unresectable </span>metastasis in melanoma patients is a major therapeutic problem.</span></p><p><span>Cold atmospheric plasma (CAP) contains electrons, charged particles, radicals, various excited molecules, UV photons, and transient electric fields. These various compositional elements have the potential either to enhance cellular activity, or to disrupt it. In particular, based on this unique composition, CAP could offer a minimally-invasive surgical approach allowing for specific cancer cell<span><span> or tumor tissue removal without influencing healthy cells. Topical application of treatment agents is a basic principle of dermatological therapy. However, the effective barrier function of the skin significantly impairs the bioavailability of most </span>topical drugs. Fractional ablative lasers represent an innovative strategy to overcome the epidermal barrier in a standardized, contact-free manner. The bioavailability of topical agents can be significantly enhanced using laser-assisted delivery. Ablative fractional laser resurfacing creates vertical channels that might assist the delivery of topically applied cold plasma into </span></span>cutaneous melanoma metastasis.</p><p>For lesions refractory to elective treatments, the laser-assisted drug delivery technique combined with cold atmospheric plasma may present a new potential option.</p><p>We report on a pilot study showing a proof of concept for enhancing topical cold atmospheric plasma permeation into depth by ablative fractional laser technique.</p></div>","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":"9 ","pages":"Page 46"},"PeriodicalIF":0.0,"publicationDate":"2018-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cpme.2017.12.071","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84584794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interaction Between Cap- Derived Singlet Oxygen And Tumor Cell Protective Catalase: Update And Chances","authors":"Georg Bauer","doi":"10.1016/j.cpme.2017.12.028","DOIUrl":"10.1016/j.cpme.2017.12.028","url":null,"abstract":"&lt;div&gt;&lt;p&gt;&lt;span&gt;&lt;span&gt;Transformation of cells from various tissues requires NADPH oxidase-dependent generation of extracellular superoxide anions. These drive the proliferation, but also cause the elimination of malignant cells through the HOCl and the NO/peroxynitrite signaling pathways. These intercellular signaling pathways induce &lt;/span&gt;apoptosis&lt;span&gt;&lt;span&gt; selectively in malignant cells, due to site-specific concerted interaction of defined reactive oxygen and nitrogen species (ROS/RNS). Tumor progression requires the expression of membrane-associated catalase. This enzyme interferes with HOCl signaling through decomposition of H2O2, and with NO/peroxynitrite signaling through &lt;/span&gt;oxidation of NO and decomposition of &lt;/span&gt;&lt;/span&gt;peroxynitrite. Membrane-associated catalase has been found on all lines of bona fide tumor cells and represents a promising target for novel antitumor strategies. Inactivation of tumor cell-specific membrane-associated catalase reactivates intercellular ROS/RNS-dependent apoptosis-inducing signaling and leads to autocrine apoptotic selfdestruction of tumor cells.&lt;/p&gt;&lt;p&gt;&lt;span&gt;&lt;span&gt;Model experiments with defined ROS and RNS led to the conclusion that CAP-derived singlet oxygen might lead to site-specific inactivation of catalase, followed by tumor cell-specific generation of secondary singlet oxygen and further inactivation of catalase. This then allows for subsequent reactivation of intercellular ROS/RNS-dependent apoptosis-inducing signaling [1]. Only on the first sight, this model seemed a) to be in contradiction to the model on the dependence of CAP action from aquaporins [2] and b) to be independent of subsequent &lt;/span&gt;immunogenic cell death&lt;span&gt; and activation of a cytotoxic T cell response [3, 4]. The analysis of existing experimental data from several groups and the alignment of site-specific mechanisms, defined by chemical biology and &lt;/span&gt;&lt;/span&gt;cell biology, allowed to establish an updated and comprehensive model [5] that includes the concepts from references [1-4] in a rational way. This model shows several biochemical amplification loops related to the generation of secondary singlet oxygen, a positive feed-back of HOCl signaling on immunogenic modulation, as well as a feedback loop from activated T cells to catalase inactivation and reactivation of intercellular ROS/RNS signaling. Thereby HOCl seems to play a role as mediator for apoptosis induction and enhancer of immunogenic stimulation.&lt;/p&gt;&lt;p&gt;&lt;span&gt;The detailed biochemical analysis&lt;span&gt; of generation of secondary singlet oxygen by tumor cells after initial interaction with exogenous singlet oxygen allowed to predict that an increase in the concentrations of tumor cell-derived extracellular superoxide anions and/or nitric oxide should cause a substantial synergistic effect with exogenous singlet oxygen. Therefore, a hybrid molecule, consisting of a plant-derived NOX stimulator and a plant-derived inhibitor of NO &lt;/span&gt;&lt;/span&gt;dioxygenase&lt;span&gt;","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":"9 ","pages":"Pages 17-18"},"PeriodicalIF":0.0,"publicationDate":"2018-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cpme.2017.12.028","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76453757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}