Clinical Plasma Medicine最新文献

{"title":"Gwu-Usmi Plasma Medicine Research Program","authors":"Jerome Canady ,&nbsp;Barry Trink ,&nbsp;Jonathan Sherman ,&nbsp;Michael Keidar","doi":"10.1016/j.cpme.2017.12.004","DOIUrl":"10.1016/j.cpme.2017.12.004","url":null,"abstract":"<div><p><span>This talk will introduce the recently established joint program on plasma cancer therapy between USMI and GWU. The use of cold atmospheric plasma-based instruments resulting from prior collaborations between USMI and GWU have already shown promise in the treatment of cancer. In January 2016, a surgical team used the Canady Helios</span>^TM<span><span> Cold Plasma Scalpel to selectively kill cancerous tissue during a two-stage liver resection in a patient with advanced inoperable liver cancer. The innovative technology allowed the surgical removal of the cancerous tissue without damaging the </span>blood supply<span> to the remaining liver. This research program will enable the transition of the novel CAP cancer treatment technology into translational and clinical stages by the development of new self-adaptive plasma devices, development of in-situ diagnostics to probe cell and tissue response to plasma action including optical, magnetic and thermal imaging, and the development of remote (non-surgical) techniques for plasma treatment.</span></span></p></div>","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cpme.2017.12.004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80935119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sensitivity Of Murine Melanoma Cells B16 To Plasma Needle Treatment","authors":"Jorge Humberto Serment Guerrero * ,&nbsp;Karina Giron Romero ,&nbsp;Régulo López-Callejas ,&nbsp;Rosendo Peña-Eguiluz","doi":"10.1016/j.cpme.2017.12.037","DOIUrl":"10.1016/j.cpme.2017.12.037","url":null,"abstract":"<div><p><span><span>Melanoma<span> is the most dangerous form of skin cancer originated from the pigment-producing melanocytes in the </span></span>basal layer<span> of the epidermis. Its capability to produce metastasis, along with the development of quimio or </span></span>radioresistance<span><span> makes this kind of cancer very dangerous and hard to heal, so is important an early detection as well as the search of new treatments. B16 murine cell line has been used extensively as a model for the study of this type of cancer. Non thermal plasma needle has been effectively used in the inactivation of microorganisms, in the disinfection of several types of tissues and to accelerate the wound healing processes. It also has been tested as an inductor of </span>apoptosis<span> in HepG2 cancer cell line<span>. The observed effects of plasma upon cells has been attributed to the generation of reactive oxygen/nitrogen species (RONS), which can disrupt the plasmatic membrane and react with several biomolecules including DNA. The aim of the present work is to evaluate the sensitivity of the murine melanoma cell line B16 to a helium-generated plasma needle exposure.</span></span></span></p><p>B16 cells were grown at 37°C and 5% CO2 atmosphere, in minimal essential medium with 10% FBS.</p><p><span>Cells were harvested by tripsinization, washed twice with Hanks balanced saline solution and further incubated for at least one hour in MEM at 37°C to recover. Blood samples were obtained from healthy donors by venopunction and lymphocytes were separated by using the Ficoll<span> Hypaque technique. Nucleated cells were collected, washed twice with HBSS, resuspended in RPMI-1640 with 10% FBS and kept at 37°C for at least one hour. Both cell types were resuspended in HBSS and then 200 µl aliquots were distributed in a microwell plate and exposed to plasma generated by a flow of 0.7 LPM of helium through a 13.56 MHz radiofrequency generator at a power of 5 W. The needle was kept at a distance of 2 mm. Cell death was evaluated by the fluorescein diacetate<span><span><span> technique. After treatments, cells were stained with a 1:1 fluorescein diacetate (80 µg/ml) and ethidium bromide (50 µg/ml) solution and observed with an epifluorescence microscope. Living cells are stained in green while dead cells are stained in red. </span>Genotoxicity was assessed by means of the </span>comet assay<span>. After treatments, cells were mixed with an equal volume of 1% low melting point agarose, poured on top of fully frosted microscope slides and immerse in cold lysis solution for an hour. Slides were then transferred to an </span></span></span></span>electrophoresis cell<span>, covered with the electrophoresis solution (0.3M NaHO, 0.1 mM Na2EDTA) for 20 minutes to allow DNA unwinding and then a current was applied (20 V, 300 mA, 20 minutes). Slides were stained with 60 µl of a 20 µg/ml ethidium bromide solution and observed under an epifluorescence microscope. Comets were score","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cpme.2017.12.037","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89757526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanosecond-Pulsed DBD Plasma For A Clinical Trial Of Actinic Keratosis","authors":"Vandana Miller MD ,&nbsp;Abraham Lin ,&nbsp;Gregory Fridman ,&nbsp;Alexander Fridman ,&nbsp;Peter Friedman MD","doi":"10.1016/j.cpme.2017.12.068","DOIUrl":"10.1016/j.cpme.2017.12.068","url":null,"abstract":"<div><p><span><span><span>Actinic keratosis<span> (AK) is a pre-cancerous skin lesion, the earliest clinical stage in a continuum of </span></span>malignancy<span><span> that may lead to squamous cell carcinoma (SCC), a skin cancer that accounts for significant morbidity (over 1 million cases in the US annually), and thousands of preventable deaths annually. This translates to a total direct cost for AK of about $1.2 billion and approximately 8.2 million office visits annually. Available treatments ablate lesions via </span>cryotherapy, </span></span>curettage<span><span> or long-term application of chemotherapeutic or immune-modulator drugs. They are typically associated with pain, scarring and inflammation, undesirable side effects that reduce patient compliance. To address these, we studied the use of non-equilibrium, atmospheric pressure plasma (NEAPP) for the treatment of AK in a small </span>clinical trial<span>. [1] One month post NEAPP treatment, over 50% of the lesions achieved full clinical resolution or showed significant improvement. Abscopal effects<span> were observed in some cases, possibly through immune stimulation. [2] Furthermore, no patient experienced adverse effects, immediate or delayed. One year follow-up showed no recurrence of any of the treated lesions. [unpublished data] 2-year follow-up results and further optimization of treatment under a new clinical trial will be presented. Our results demonstrate that NEAPP may serve as a potential, effective treatment for AKs.</span></span></span></span><span><figure><span><img><ol><li><span>Download : <span>Download high-res image (166KB)</span></span></li><li><span>Download : <span>Download full-size image</span></span></li></ol></span></figure></span></p><p>Evaluation of nspDBD treatment of AK. 9 lesions fully resolved, 3 showed significant improvement</p></div>","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cpme.2017.12.068","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77347629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"OBC","authors":"","doi":"10.1016/S2212-8166(18)30009-X","DOIUrl":"https://doi.org/10.1016/S2212-8166(18)30009-X","url":null,"abstract":"","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S2212-8166(18)30009-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"137433022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Generation Of Reactive Species By Plasma Needle In Different Liquids","authors":"Inés Hamouda ,&nbsp;Alix Gouhier ,&nbsp;Cédric Labay ,&nbsp;Maria-Pau Ginebra ,&nbsp;Cristina Canal","doi":"10.1016/j.cpme.2017.12.054","DOIUrl":"10.1016/j.cpme.2017.12.054","url":null,"abstract":"<div><p>Plasma activated media (PAM) is produced by exposing liquids to cold atmospheric plasmas [1,2]. PAM has gained increasing attention due to its capacity to kill cancer cells<span> as effectively as direct treatment of cells in culture by cold plasmas.</span></p><p>In previous works [3] we could show the selectivity of an atmospheric pressure plasma needle on osteosarcoma cell lines versus healthy bone cells. The cytotoxicity of the direct plasma treatment on cells was comparable to treatment with PAM, wherein in this case the liquid selected was cell culture medium.</p><p>The rationale beyond employing PAM lies in being able to avoid the effects of electrical field, or UV/VIS radiation present in plasmas, and its biological effects seem to lay in the reactive oxygen species<span> (ROS) and reactive nitrogen species (RNS) generated in the aqueous state. The concentration of ROS and RNS in the PAM is directly related to its effectiveness in killing cancer cells. It is our interest to evaluate the different parameters influencing the generation of ROS and RNS.</span></p><p>In this work different liquid media (different cell culture media, aqueous solutions such as water or ringer’s saline, etc.) are compared, and the production of ROS and RNS is quantified in different conditions (volume of liquid, treatment times, distance to the nozzle). The stability of the mentioned species is evaluated with time.<span><figure><span><img><ol><li><span>Download : <span>Download high-res image (106KB)</span></span></li><li><span>Download : <span>Download full-size image</span></span></li></ol></span></figure></span></p><p>Figure 1. Production of H₂O₂ and NO₂^- by APPJ treatment on different cell culture medium (a) McCoy and (b) AdvDMEM.</p></div>","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cpme.2017.12.054","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85083567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combining cold physical plasma with pulsed electrical fields for cancer treatment","authors":"Christina Maria Wolff,&nbsp;Anna Steuer,&nbsp;Jürgen F. Kolb,&nbsp;Sander Bekeschus","doi":"10.1016/j.cpme.2017.12.042","DOIUrl":"10.1016/j.cpme.2017.12.042","url":null,"abstract":"<div><p><span><span><span>Pallation of end-stage melanoma<span> and breast cancer patients is a challenge. Despite the generally successful use of electrochemotherapy (ECT) in these patients, response rates can still be improved. Previous studies demonstrated the anticancer potential of nano-second PEFs (nsPEFs), which are able to induce </span></span>apoptosis [1] or </span>nanopore formation when using different settings [2]. Likewise, the anticancer capacity of cold physical plasma has been demonstrated in various studies in cell lines and in </span>xenograft tumors<span><span> in mice [3-7]. The apoptotic effect of cold plasmas is mediated by a variety of reactive species being released onto the cells, where the species trigger redox signaling, and subsequent cell death in some instances [8, 9]. By contrast, PEFs act by a mechanism much less dependent on the newly generated reactive species. Our idea was to combine both </span>treatments to improve palliative cancer treatment in the future.</span></p><p><span>Malignant suspension cell lines were tested to investigate the proof of concept of additive or possible even synergistic cytotoxic effects. Plasma treatment time (kINPen) and PEF intensity as well as pulse length were varied to retrieve sublethal dosage regimens<span><span> for each treatment. The sequence of combination (first plasma, then PEF or vice versa) was also modulated. To investigate the mode of action of both therapies, a number of cellular parameters were investigated. This included oxidation at cytosolic and membrane compartments, thiol content, mitochondrial depolarization, </span>caspase activation and </span></span>phosphatidylserine<span> exposure, metabolic activity, cell membrane<span> permeabilization, cell growth and morphology, and protection by antioxidants. Furthermore, we identified a synergistic effect of plasma and PEFs using tumorigenic adherent cells in preliminary tests.</span></span></p></div>","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cpme.2017.12.042","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90809666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Introducing Asia-Pacific Plasma Oncology Network: Progress On Triple Negative Breast Cancer","authors":"Xiaofeng Dai ,&nbsp;Kostya Ostrikov","doi":"10.1016/j.cpme.2017.12.043","DOIUrl":"10.1016/j.cpme.2017.12.043","url":null,"abstract":"<div><p>Here we introduce the Asia-Pacific Plasma Oncology network representing researchers based in Australia, China, USA, Korea, Malaysia and Singapore and welcome other members to join.</p><p>Triple negative breast cancers (TNBC), among the many subtypes of such cancers, have the worst prognosis due to lack of surficial marker expression and effective targeted therapy as well as highest aggressiveness and cancer stemness. Plasma activated medium (PAM) was found, from our work, to be more effective in selectively killing TNBCs and was applied in the following three lines of studies.</p></div><div><h3>Part 1: Establishment of computational equation predicting cells’ response to PAM</h3><p>We designed orthogonal experiments to analyze the effects of various parameters of PAM in treating TNBC cells. Among 7 independent parameters, i.e., cell number (C), treatment time (T), output voltage (U), helium flow rate (F), well size (A), distance from the tail of plasma jet to medium surface (D1), and medium thickness (D2), we identified 4 primary influential factors that collectively determine the efficacy of PAM in treating TNBC.</p></div><div><h3>Part 2: Internal plasma treatment device development and efficacy comparison</h3><p>Novel plasma needle-like punctuation approach and device were developed for internal animal treatment, using which our <em>in vivo</em> experiments demonstrate the advantages of internal plasma treatment over PAM and surficial treatment.</p></div><div><h3>Part 3: Exploration on mechanisms leading to PAM selectivity in TNBC treatment</h3><p>PAM was found capable of selectively killing and inhibiting the migration ability of TNBC cells as compared with other BC subtypes. The cell cohort with ALDH over-expression, representing cancer stem cells, was found to be more sensitive to PAM treatment. High throughput sequencing on TNBC and non-TNBC cell lines treated with PAM under different time points revealed a dynamic network integrating data at mRNA, miRNA, lncRNA and circRNA levels. Pathway analyses consolidate our hypothetical network where ALDH1 mediated cancer stem cell signaling is more sensitive to PAM treatment that leads to higher fraction of cell death among cancer stem cells than bulk tumor cells.</p><p>Our current network involves more than 20 active faculty members from Australia, China, Korea, USA, Singapore and Malaysia with their researchers and students, and we sincerely thank all of them.</p></div>","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cpme.2017.12.043","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86646481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of RAC1 Gene Expression Under Exposure Of Plasma Activated Verbascoside in HT-29 Colorectal Cancer Cells","authors":"Kobra Hajizadeh ,&nbsp;Bahram Behzad ,&nbsp;Danial Seifi ,&nbsp;Hassan Mehdian ,&nbsp;Mohammad Nabiouni ,&nbsp;Kamal Haji-Sharifi ,&nbsp;Elahe Amini","doi":"10.1016/j.cpme.2017.12.057","DOIUrl":"10.1016/j.cpme.2017.12.057","url":null,"abstract":"<div><p>Colon cancer is one of the most common and most prevalent Neoplasma in men; adenocarcinomas make up 95 percent of all colon cancer cases. Due to the side effects of conventional treatment methods such as chemotherapy, today, attentions have been attracted to natural compounds that have less side effects and anticancer properties.</p><p>This study aims to investigate the effect of Verbascoside as a natural compound in inhibiting HT-29 signaling pathway which is an effective route in the metastasis, angiogenesis and cell migration in the human cell line of HT-29 colon cancer; and also investigates the possibility of enhancing and raising the effect of this herbal drug by cold atmospheric pressure plasma jet.</p><p>A the first step, HT-29 cells were treated at different concentrations of verbascoside 10, 20, 30, 40, 50, 70, 100 μg / ml for 24 hours, then MTT test was used to calculate the inhibitory concentration. The colon cancer cell migration was evaluated by scratch test and HIF-1α gene expression was evaluated by Real Time PCR. The results of the MTT assay showed that verboscoside dose-dependent inhibition of cancer cells. The HT-29 colon is at a concentration of 50 μg / ml. Scratch repair tests showed that verboscoside reduced the migration of cells in a dose-dependent manner, and the results of Real Time PCR showed a decrease in the expression of HIF-1α gene expression. Cancer tumors undergo some changes in oxygen deficiency conditions, including increasing the expression of the HIF-1α gene, which in turn play an important role in activating and enhancing the expression of genes involved in metastasis, angiogenesis and migration.</p><p>At the second step of this study, verboscoside was primarily treated by cold atmospheric plasma jet then the same procedure has been taken for plasma activated verboscoside. In this study helium jet has been used.</p><p>The findings of this study showed that verboscosid with inhibitory expression of HIF-1α has a significant anti-metastatic, cellular angiogenesis and migration effect and may be a good option for reducing metastasis and angiogenesis in colon cancer models; it has been also showed that plasma activated verboscosid is more efficacious in all of the above mentioned features.</p></div>","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cpme.2017.12.057","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89402893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Plasma Activated Verbascoside on HIF-1A Expression as Valuable Metastasis and Angiogenesis factor in HT-29 Colon Cancer Cells","authors":"Kobra Hajizadeh ,&nbsp;Bahram Behzad ,&nbsp;Danial Seifi ,&nbsp;Hassan Mehdian ,&nbsp;Mohammad Nabiouni ,&nbsp;Kamal Haji-Sharifi ,&nbsp;Elahe Amini","doi":"10.1016/j.cpme.2017.12.056","DOIUrl":"10.1016/j.cpme.2017.12.056","url":null,"abstract":"<div><p>Colon cancer<span> is one of the most common and most prevalent Neoplasma in men; adenocarcinomas make up 95 percent of all colon cancer cases. Due to the side effects of conventional treatment methods such as chemotherapy, today, attentions have been attracted to natural compounds that have less side effects and anticancer properties.</span></p><p><span>In attempting to investigate factors which may account for cancer treatment, this study raises two interrelated questions. Firstly, what is the effect of Verbascoside<span><span> as a natural compound in inhibiting HIF-1α signaling pathway which is an effective route in the </span>metastasis<span>, angiogenesis and cell migration in the human cell line of HT-29 colon cancer; second, is it possible to enhance and raise the effect of this </span></span></span>herbal drug by cold atmospheric pressure plasma jet?</p><p>The focus of this study will be investigating the effect of Verbascoside as a natural compound in inhibiting HIF-1α signaling pathway and also examining the effect of atmospheric pressure plasma on the function of Verbascoside.</p><p><span>A the first step, 29-HT cells were treated at different concentrations of verbascoside 10, 20, 30, 40, 50, 70, 100 μg / ml for 24 hours, then MTT test was used to calculate the inhibitory concentration. The colon cancer cell migration was evaluated by scratch test and HIF-1α gene expression was evaluated by Real Time PCR. The results of the </span>MTT assay<span> showed that verboscoside dose-dependent inhibition of cancer cells<span>. The HT-29 colon is at a concentration of 50 μg/ml. Scratch repair tests showed that verboscoside reduced the migration of cells in a dose-dependent manner, and the results of Real Time PCR showed a decrease in the expression of HIF-1α gene expression. Cancer tumors undergo some changes in oxygen deficiency conditions, including increasing the expression of the HIF-1α gene, which in turn play an important role in activating and enhancing the expression of genes involved in metastasis, angiogenesis and migration.</span></span></p><p>At the second step of this study, verboscoside was primarily treated by cold atmospheric plasma jet then the same procedure has been taken for plasma activated verboscoside. In this study helium jet has been used.</p></div>","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cpme.2017.12.056","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89783859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-Thermal Plasma As An Innovative Anticancer Strategy On Leukemia Models","authors":"Eleonora Turrini ,&nbsp;Augusto Stancampiano ,&nbsp;Emanuele Simoncelli ,&nbsp;Romolo Laurita ,&nbsp;Elena Catanzaro ,&nbsp;Cinzia Calcabrini ,&nbsp;Matteo Gherardi ,&nbsp;Vittorio Colombo ,&nbsp;Carmela Fimognari","doi":"10.1016/j.cpme.2017.12.025","DOIUrl":"10.1016/j.cpme.2017.12.025","url":null,"abstract":"<div><p><span><span>Antitumor chemotherapy is often hampered by the low therapeutic index of most anticancer drugs and the development of chemoresistance. Furthermore, in leukemia incidence and death rates </span>in patients<span> are really close to each other, when compared to other kind of tumors; pointing out that efficacy of anticancer therapy<span><span> is suboptimal. Thus, there is a continuous need for new intervention strategies, endowed with a better pharmaco-toxicological profile. Cold atmospheric plasma (CAP) has gained interest as a promising anticancer strategy and earlier studies demonstrated the “non-aggressive” nature of CAP [1]. Several lines of evidence showed that the anticancer activity of CAP mainly depends on the increase in oxidative and nitrosative stress that leads to tumor </span>cell death [2]. However, mechanisms of CAP-cell interaction are not yet completely understood. In this context, the aim of this work is to unravel CAP anticancer effects on </span></span></span><em>in vitro</em> and <span><em>ex vivo</em></span><span><span><span> leukemia models achieved within the Italian national project “Scientific Independence for young Researchers” (SIR), that brings together a multidisciplinary team in the areas of Engineering, Pharmacology and Oncohematology. The cytotoxic impact of different CAP treatments performed by means of two plasma sources, a nanosecond pulsed </span>dielectric<span> barrier discharge (DBD) [3,4] and a microsecond pulsed DBD jet (Fig.1), on T-lymphoblastic cell lines was investigated. In particular, we analyzed apoptotic and/or necrotic events, cell-cycle progression, levels of proteins involved in the regulation of apoptosis correlated to reactive oxygen and nitrogen species (RONS) induced in culture medium by CAP treatment. Due to the key role of RONS in the biological effects of CAPs, its genotoxic potential was assessed. Furthermore, some preliminary results indicate that CAPs can induce cytotoxic effects also on leukemia cells cultivated in </span></span>hypoxia, which plays a critical role in promoting chemoresistance. Taken together, the results we obtained contribute to understand the pharmaco-toxicological potential of CAP, thus making the basis to further investigate its anticancer properties.</span><span><figure><span><img><ol><li><span>Download : <span>Download high-res image (66KB)</span></span></li><li><span>Download : <span>Download full-size image</span></span></li></ol></span></figure></span></p><p>Figure 1: Nanosecond pulsed DBD on a grounded plate (right) and microsecond pulsed DBD jet (left).</p></div>","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cpme.2017.12.025","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91268500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}