Tzu Chi Medical Journal最新文献

Pathophysiology and potential treatment modalities in women with recurrent urinary tract infection. 女性复发性尿路感染的病理生理学和潜在的治疗方式。

IF 1.4

Tzu Chi Medical Journal Pub Date : 2025-04-07 eCollection Date: 2025-04-01 DOI: 10.4103/tcmj.tcmj_286_24

Wei-Ju Liao, Yuan-Hong Jiang, Jia-Fong Jhang, Sheng-Fu Chen, Yu Khun Lee, Cheng-Ling Lee, Tien-Lin Chang, Hann-Chorng Kuo

{"title":"Pathophysiology and potential treatment modalities in women with recurrent urinary tract infection.","authors":"Wei-Ju Liao, Yuan-Hong Jiang, Jia-Fong Jhang, Sheng-Fu Chen, Yu Khun Lee, Cheng-Ling Lee, Tien-Lin Chang, Hann-Chorng Kuo","doi":"10.4103/tcmj.tcmj_286_24","DOIUrl":"https://doi.org/10.4103/tcmj.tcmj_286_24","url":null,"abstract":"Urinary tract infection (UTI) of the urinary bladder is a common bacterial infection that predominantly affects women, with many experiencing recurrent episodes. Recurrent UTIs (rUTIs) are associated with significant physical, psychological, and social difficulties. Further, they are closely related to lower urinary tract dysfunction (LUTD). LUTD affects bladder function and structure, thereby contributing to urinary urgency, frequency, and incontinence, which, in turn, increases the risk of recurrent infections due to impaired urothelial defense mechanisms. The current study explored the pathophysiology of LUTD in women with rUTIs. Potential treatments for rUTIs include long-term prophylactic antibiotics, probiotics, D-mannose, vaccines, small molecule inhibitors, and stem cell therapy. Moreover, it evaluated the use of platelet-rich plasma (PRP) therapy as a treatment modality for LUTD. PRP has regenerative and anti-inflammatory properties. Hence, it can be a promising option for enhancing urothelial barrier integrity and reducing infection recurrence. Repeated intravesical PRP injections are effective in improving bladder symptoms and decreasing UTI recurrences by enhancing the proliferative ability of the urothelium in patients with rUTIs. Further, this review examined the potential predictors of successful PRP treatment outcomes such as cytokine and urothelial biomarker levels, which provided insights into patient selection and individualized treatment strategies. Identifying the predictive biomarkers of treatment responsiveness is essential for optimizing PRP therapy. Hence, to improve the clinical outcomes and quality of life of patients with rUTIs, future research should focus on refining the use of PRP, exploring combination therapies, and validating biomarkers.","PeriodicalId":45873,"journal":{"name":"Tzu Chi Medical Journal","volume":"37 2","pages":"117-124"},"PeriodicalIF":1.4,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12048121/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144040882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rare APOE p.Gly4Glu: A putative disease-causing variant for early-onset Alzheimer's disease identified by next-generation sequencing. 罕见APOE p.Gly4Glu：通过下一代测序确定的早发性阿尔茨海默病的推定致病变异

IF 1.4

Tzu Chi Medical Journal Pub Date : 2025-04-07 eCollection Date: 2025-04-01 DOI: 10.4103/tcmj.tcmj_117_24

Chu-Ting Wu, Liang-Hsuan Hu, Hui-Ying Weng, Yen-Ming Liu, Yung-Feng Lin, Shih-Feng Tsai, Raymond Y Lo, Yung-Hao Ching

{"title":"Rare APOE p.Gly4Glu: A putative disease-causing variant for early-onset Alzheimer's disease identified by next-generation sequencing.","authors":"Chu-Ting Wu, Liang-Hsuan Hu, Hui-Ying Weng, Yen-Ming Liu, Yung-Feng Lin, Shih-Feng Tsai, Raymond Y Lo, Yung-Hao Ching","doi":"10.4103/tcmj.tcmj_117_24","DOIUrl":"https://doi.org/10.4103/tcmj.tcmj_117_24","url":null,"abstract":"Objectives: We aimed to identify the early-onset Alzheimer's disease (EOAD)-causing variants in the Eastern Taiwanese population.Materials and methods: Twenty-one patients diagnosed with EOAD in the memory clinic at Hualien Tzu Chi Hospital were enrolled during 2014-2018. We conducted whole-exome sequencing to identify the disease-causing variations and validated by Sanger sequencing. SIFT, PolyPhen-2, and AlphaFold were applied to predict the functional impact of the identified variants.Results: Two unrelated normolipidemic EOAD patients were carrying a rare heterozygous APOE variant (rs373985746, NC_000019.10:g. 44905879G>A, NM_001302688.2:c. 11G>A, and NP_001289617.1:p.Gly4Glu) with the allele frequency as 0.000206. Sanger sequencing uncovered the ∑ haplotypes in which the c.11G>A variation resided. SIFT predicted that the variant severely impacts protein structure and, maybe thus, function. AlphaFold predicted a dysfunctional conformation of the mutant APOE precursor a protein (p.Gly4Glu).Conclusion: Our data strongly suggest that the rare p.Gly4Glu variant is associated with EOAD but does not cause dyslipidemia.","PeriodicalId":45873,"journal":{"name":"Tzu Chi Medical Journal","volume":"37 2","pages":"175-180"},"PeriodicalIF":1.4,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12048122/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144020646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

3-Hydroxy-3-methylglutaryl-CoA reductase variants strongly associated with low-density lipoprotein cholesterol levels and diabetes mellitus risk in a Taiwanese population: A Mendelian randomization study. 3-羟基-3-甲基戊二酰辅酶a还原酶变异与台湾人群低密度脂蛋白胆固醇水平和糖尿病风险密切相关：孟德尔随机研究。

IF 1.4

Tzu Chi Medical Journal Pub Date : 2025-04-07 eCollection Date: 2025-04-01 DOI: 10.4103/tcmj.tcmj_247_24

Lung-An Hsu, Ming-Sheng Teng, De-Min Duan, Kuan-Hung Yeh, Semon Wu, Yu-Lin Ko

{"title":"3-Hydroxy-3-methylglutaryl-CoA reductase variants strongly associated with low-density lipoprotein cholesterol levels and diabetes mellitus risk in a Taiwanese population: A Mendelian randomization study.","authors":"Lung-An Hsu, Ming-Sheng Teng, De-Min Duan, Kuan-Hung Yeh, Semon Wu, Yu-Lin Ko","doi":"10.4103/tcmj.tcmj_247_24","DOIUrl":"https://doi.org/10.4103/tcmj.tcmj_247_24","url":null,"abstract":"Objectives: 3-Hydroxy-3-methylglutaryl-CoA reductase (HMGCR) is a rate-limiting enzyme involved in cholesterol synthesis. The common HMGCR variants are associated with low-density lipoprotein cholesterol (LDL-C) levels. We aimed to identify novel HMGCR variants influencing the lipid profiles of Taiwanese and assess the causal links between LDL-C levels and diabetic risk based on HMGCR genotypes.Materials and methods: Genome-wide genotyping of 108,880 participants from Taiwan Biobank was used for the association studies and Mendelian randomization (MR) analysis.Results: Regional association and stepwise linear regression analyses showed HMGCR rs3064191, rs150454634, and rs13354746 variants were independently associated with total cholesterol (TC), LDL-C, and non-high-density lipoprotein cholesterol (non-HDL-C) levels with the former two variants in strong linkage disequilibrium with HMGCR rs3846662, a variant influencing exonal alternative splicing, and HMGCR rs191835914 (p.Y311S), an Asian-specific nonsynonymous mutation, respectively. Multivariate MR analyses showed significant associations between weighted genetic risk scores using LDL-C-determining HMGCR variants and using genome-wide association study identifying LDL-C-determining 47 variants and the prevalence of diabetes mellitus (DM) (P = 0.0011 and P = 1.66 × 10-8, respectively).Conclusion: The HMGCR variants exhibited significant associations with TC, LDL-C, and non-HDL-C levels as well as causally with DM risk in our Taiwanese population. HMGCR genotypes may play an important role and serve as a reference for the prevention and treatment of cardiovascular diseases in the clinical settings.","PeriodicalId":45873,"journal":{"name":"Tzu Chi Medical Journal","volume":"37 2","pages":"181-188"},"PeriodicalIF":1.4,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12048127/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144024281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dietary supplementation roles in concussion management: A systematic review. 膳食补充在脑震荡管理中的作用：一项系统综述。

IF 1.4

Tzu Chi Medical Journal Pub Date : 2025-04-07 eCollection Date: 2025-04-01 DOI: 10.4103/tcmj.tcmj_241_24

Andre Marolop Pangihutan Siahaan, Alvin Ivander, Bahagia Wilibrordus Maria Nainggolan, Ruth Hasian Nami Siagian, Lidya Veronika, Natanael Ramoti

{"title":"Dietary supplementation roles in concussion management: A systematic review.","authors":"Andre Marolop Pangihutan Siahaan, Alvin Ivander, Bahagia Wilibrordus Maria Nainggolan, Ruth Hasian Nami Siagian, Lidya Veronika, Natanael Ramoti","doi":"10.4103/tcmj.tcmj_241_24","DOIUrl":"https://doi.org/10.4103/tcmj.tcmj_241_24","url":null,"abstract":"Concussion, one of the most common types of mild traumatic brain injury, remains a global problem that poses substantial effects on individuals, families, and society. When dealing with concussion, clinicians primarily focus on symptomatic treatment and modified activity with no established therapies specifically addressing the underlying pathophysiological changes. In recent years, there has been a growing increase in attention to the effectiveness of dietary supplements (DS) and nutritional interventions as adjunctive therapy options for concussion. Hence, this review aims to comprehensively explore the existing human studies on using DS as adjunctive therapy in the management of concussion. This systematic review adhered to the Preferred Reporting Items for Systematic Reviews and Meta-analyses guidelines. The search strategy was created based on the population, intervention, comparison, outcome framework. The findings are conveyed narratively and analyzed according to the timing of the intervention. DS administered within 7 days of onset were classified as acute interventions, while those given after this period were classified as nonacute interventions. After screening, we identified 21 reports for 19 studies involving 13 DS. Thirteen DS were included in this review. Notably, omega-3 polyunsaturated fatty acids were the most extensively studied and accounted for 23.81% of studies, followed by melatonin and pine bark extract (19.05% and 9.5%). At least 13 supplements were identified in clinical studies, with 77% demonstrating favorable outcomes. However, none of the interventions reviewed offer strong enough evidence to justify regular use in clinical practice.","PeriodicalId":45873,"journal":{"name":"Tzu Chi Medical Journal","volume":"37 2","pages":"157-166"},"PeriodicalIF":1.4,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12048115/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144019884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Endothelial dysfunction in chronic kidney disease: Mechanisms, biomarkers, diagnostics, and therapeutic strategies. 慢性肾脏疾病的内皮功能障碍：机制、生物标志物、诊断和治疗策略。

IF 1.4

Tzu Chi Medical Journal Pub Date : 2025-03-21 eCollection Date: 2025-04-01 DOI: 10.4103/tcmj.tcmj_284_24

Ji-Hung Wang, Yu-Li Lin, Bang-Gee Hsu

{"title":"Endothelial dysfunction in chronic kidney disease: Mechanisms, biomarkers, diagnostics, and therapeutic strategies.","authors":"Ji-Hung Wang, Yu-Li Lin, Bang-Gee Hsu","doi":"10.4103/tcmj.tcmj_284_24","DOIUrl":"https://doi.org/10.4103/tcmj.tcmj_284_24","url":null,"abstract":"Endothelial cells regulate vascular tone, blood flow, coagulation, and inflammation, with heterogeneous populations serving specific roles throughout the body. In the kidney, endothelial cells maintain vascular integrity and function, contribute to filtration, and support other renal structures. Nitric oxide (NO) is a key signaling molecule that maintains vascular tone and endothelial function. It is synthesized by nitric oxide synthase (NOS) isoforms, with endothelial NOS playing a central role in vascular health. Chronic kidney disease (CKD) is characterized by reduced NO bioavailability, driven by the accumulation of endogenous NOS inhibitors such as asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA). Uremic toxins, oxidative stress, and proinflammatory cytokines contribute to a prothrombotic and proinflammatory state, contributing to endothelial dysfunction and exacerbating cardiovascular (CV) risks in CKD. Biomarkers such as ADMA, SDMA, endothelial microparticles, and soluble adhesion molecules offer insights into vascular health, while invasive or noninvasive diagnostic techniques can assess endothelial function in CKD. Effective management strategies focus on enhancing NO bioavailability, controlling oxidative stress, reducing inflammation, and optimizing dialysis to minimize uremic toxin levels. Emerging therapeutic approaches, including antioxidant therapies and endothelial progenitor cell-based interventions, show promise in preserving vascular function. A multifaceted approach to managing endothelial dysfunction is critical for mitigating CV complications and improving patient outcomes in CKD.","PeriodicalId":45873,"journal":{"name":"Tzu Chi Medical Journal","volume":"37 2","pages":"125-134"},"PeriodicalIF":1.4,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12048126/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144038688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cardiac computed tomography: Current practice, guidelines, applications, and prospects. 心脏计算机断层扫描：目前的实践、指南、应用和前景。

IF 1.4

Tzu Chi Medical Journal Pub Date : 2025-03-05 eCollection Date: 2025-04-01 DOI: 10.4103/tcmj.tcmj_125_24

Yu-Tai Shih, Jia-Hao Zhou, Jong-Kai Hsiao

{"title":"Cardiac computed tomography: Current practice, guidelines, applications, and prospects.","authors":"Yu-Tai Shih, Jia-Hao Zhou, Jong-Kai Hsiao","doi":"10.4103/tcmj.tcmj_125_24","DOIUrl":"https://doi.org/10.4103/tcmj.tcmj_125_24","url":null,"abstract":"Cardiac computed tomography (CT) has evolved significantly as a critical tool in diagnosing and managing cardiac diseases, greatly facilitated by technological advancements in multidetector systems, dose-reduction techniques, and sophisticated imaging algorithms. This article discusses the historical progression and technological evolution in cardiac CT (CCT), focusing on the impact of 64-multidetector row CT and dual-energy CT systems on improving spatial and temporal resolutions and reducing radiation exposure. It explores the role of these technologies in enhancing diagnostic accuracy, such as through detailed three-dimensional reconstructions and minimized imaging artifacts. Furthermore, it highlights the integration of machine learning to automate complex imaging analysis and photon-counting CT, which promises higher resolution and further dose reduction. Prospective studies and ongoing trials such as FASTTRACK coronary artery bypass grafting also underscore the potential of advanced CT technologies in refining procedural planning and execution. The continuous advancements in detector technology, computational techniques, and image reconstruction are poised to expand the applications and efficacy of CCT, cementing its role in modern cardiology.","PeriodicalId":45873,"journal":{"name":"Tzu Chi Medical Journal","volume":"37 2","pages":"145-151"},"PeriodicalIF":1.4,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12048117/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144064798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Narrative medicine in pediatric medical education and patient care: A scoping review. 叙事医学在儿科医学教育和病人护理：范围综述。

IF 1.4

Tzu Chi Medical Journal Pub Date : 2025-03-04 eCollection Date: 2025-04-01 DOI: 10.4103/tcmj.tcmj_181_24

Ting-Chun Tseng, Pan-Yuan Kuo, Meei-Ju Lin, Shao-Yin Chu

{"title":"Narrative medicine in pediatric medical education and patient care: A scoping review.","authors":"Ting-Chun Tseng, Pan-Yuan Kuo, Meei-Ju Lin, Shao-Yin Chu","doi":"10.4103/tcmj.tcmj_181_24","DOIUrl":"https://doi.org/10.4103/tcmj.tcmj_181_24","url":null,"abstract":"Narrative medicine is an approach centered on patients' experiences and their illness stories. Its application in pediatric population warrants further research. This study aims to overview (1) the application of narrative medicine in pediatric medical education and (2) the implementation of narrative medicine in pediatric patient care. Searches were conducted in bibliographic databases (Cochrane Library, ClinicalTrials.gov, EBSCOhost, Embase, MedEdPORTAL, Ovid, PubMed, and Web of Science) without date or language restrictions. Researchers independently screened articles, charted data, and performed data synthesis. Educational intervention studies were evaluated with Kirkpatrick Scale, and Mixed Methods Appraisal Tool was used for methodological quality assessment of all studies. Forty-one articles met our criteria: 17 focused on the application of narrative medicine in pediatric medical education and 24 on its implementation in pediatric patient care. Educational interventions indicated general satisfaction, with reported improvements in empathy, reflection, patient understanding, and professional development. In patient care settings, narrative medicine provided a safe space for story sharing, enhancing emotional, relational, and social connections, and promoting patient- and family-centered care. This review highlights the translation of narrative medicine from educational interventions to clinical practice in pediatric settings. Although more robust research is required, existing evidence supports narrative medicine's potential to foster authentic engagement with patients and caregivers and enhance the quality of pediatric care across various conditions and developmental stages.","PeriodicalId":45873,"journal":{"name":"Tzu Chi Medical Journal","volume":"37 2","pages":"167-174"},"PeriodicalIF":1.4,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12048120/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144054344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unmet needs of metabolic dysfunction - Associated "fatty or steatotic" liver disease. 未满足代谢功能障碍的需要-相关的“脂肪或脂肪变性”肝病。

IF 1.4

Tzu Chi Medical Journal Pub Date : 2025-03-04 eCollection Date: 2025-04-01 DOI: 10.4103/tcmj.tcmj_232_24

Yu-Ming Cheng, Shao-Wen Wang, Ching Wang, Chia-Chi Wang

引用次数: 0

An exploration of the natural and acquired immunological mechanisms to high-risk human papillomavirus infection and unmasking immune escape in cervical cancer: A concise synopsis. 探索高危人乳头瘤病毒感染的自然和获得性免疫机制，揭示宫颈癌的免疫逃逸：简要概述。

IF 1.4

Tzu Chi Medical Journal Pub Date : 2024-12-03 eCollection Date: 2025-01-01 DOI: 10.4103/tcmj.tcmj_134_24

Mohammed Mohsen Mohammed, Zaytoon Abdulrida Ighewish Al-Khafaji, Nadia Mudher Al-Hilli

{"title":"An exploration of the natural and acquired immunological mechanisms to high-risk human papillomavirus infection and unmasking immune escape in cervical cancer: A concise synopsis.","authors":"Mohammed Mohsen Mohammed, Zaytoon Abdulrida Ighewish Al-Khafaji, Nadia Mudher Al-Hilli","doi":"10.4103/tcmj.tcmj_134_24","DOIUrl":"10.4103/tcmj.tcmj_134_24","url":null,"abstract":"The most common STD that triggers cervical cancer is the human papillomavirus. More than 20 types of human papillomavirus (HPV) can induce uterine cervical cancer. Almost all women acquire genital HPV infection soon after their first intercourse, with most of them clearing the virus within 3 years. An immune response is necessary to clear. The first responders to HPV infection are the innate immune system elements composed of macrophages, keratinocytes, natural killer cells, and natural killer T-lymphocytic (NKT) cells. Cytotoxic T lymphocytes (CTLs) comprise the second line of defense and kill HPV16-infected cells expressing various peptides derived from their transforming early viral oncoproteins, mainly E2•E6. Even though HPV can manage to trick away our immune systems, first of all, it is important to emphasize that HPV replication does not kill the host cells. It does not replicate viral antigens or cause inflammation. The HPV16 E6 and E7 genes suppress host cell type 1 interferons (IFNs), which are detectable after infection. The patient may have immunological tolerance; hence, there are no costimulatory signals from inflammatory cytokines like IFNs during antigen recognition. Evidence shows that HlA class I generations have been inhibited by HPV16 E5, which could protect this tumor cell from CTL attack. HPV16 E7 is responsible for initiating immunotolerance and increasing regulatory T cells (Treg) to repress immunological regression. Evasion from immune system protection plays a critical role in the outcome of persistent HPV infection and the development of cervical cancer. Vaccination against HPV16 and 18 during adolescence is the most effective method for preventing cervical cancer in women, considering the immunological processes involved.","PeriodicalId":45873,"journal":{"name":"Tzu Chi Medical Journal","volume":"37 1","pages":"28-41"},"PeriodicalIF":1.4,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11753526/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143029685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A simple, economical, and high-yield method for polyethylene glycol-based extraction of follicular and serum-derived extracellular vesicles. 一种简单、经济、高产的聚乙二醇提取卵泡和血清来源的细胞外囊泡的方法。

IF 1.4

Tzu Chi Medical Journal Pub Date : 2024-10-25 eCollection Date: 2025-01-01 DOI: 10.4103/tcmj.tcmj_160_24

Aye Aye Khine, Pao-Chu Chen, Ying-Hsi Chen, Sung-Chao Chu, Yu-Shuan Chen, Hsuan-Shun Huang, Tang-Yuan Chu

{"title":"A simple, economical, and high-yield method for polyethylene glycol-based extraction of follicular and serum-derived extracellular vesicles.","authors":"Aye Aye Khine, Pao-Chu Chen, Ying-Hsi Chen, Sung-Chao Chu, Yu-Shuan Chen, Hsuan-Shun Huang, Tang-Yuan Chu","doi":"10.4103/tcmj.tcmj_160_24","DOIUrl":"10.4103/tcmj.tcmj_160_24","url":null,"abstract":"Objectives: The optimization of polyethylene glycol (PEG)-based extracellular vesicles (EVs) extraction from human follicular fluid (FF) and serum was investigated, and their functional analysis was confirmed. The PEG-based EV results were compared to the ExoQuick (ExoQ)-based EV.Materials and methods: FF-EVs and serum-EVs were extracted by using different concentrations of PEG (8000). Nanoparticle tracking analysis was used to count the particles, and electron microscopy of EVs was performed for visualization. Exosomes were confirmed by the western blot analysis with exosome-specific markers. RNA and microRNA were extracted from exosomes and quantitative polymerase chain reaction analysis was performed. Fallopian tube epithelial (FTE) cells were used for the EV uptake experiment and an anchorage-independent growth test to confirm that extracted EVs harbor transformation activity.Results: The PEG 8% enriched method produced the highest yield and the lowest carry-over protein. Salt containing PEG 8% produced a higher yield than nonsalted PEG 8%. Overnight enrichment increased four times and 18 times for PEG 8% and ExoQ-based EV extraction from FF. For serum EV, the same overnight enrichment moderately increased yield for both PEG 8% and ExoQ methods. Less carry-over protein resulted in more EV-promoted transformation activity.Conclusion: This study overcomes the time-consuming, expensive, laborious, and complicated machine-dependent EV extraction methods. The study highlights that longer incubation time is needed for EV extraction from FF. PEG 8000-based EV extraction provided a higher yield and less carry-over protein than ExoQ-based EV extraction.","PeriodicalId":45873,"journal":{"name":"Tzu Chi Medical Journal","volume":"37 1","pages":"49-57"},"PeriodicalIF":1.4,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11753524/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143029620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0