Journal of Oral Biosciences最新文献

{"title":"Complete genome sequence of a tetracycline-resistant Streptococcus mutans strain carrying the tet(M) gene","authors":"Saki Nishihama ,&nbsp;Yujin Suzuki ,&nbsp;Tomoaki Shintani ,&nbsp;Manami Tsunoi ,&nbsp;Junzo Hisatsune ,&nbsp;Yo Sugawara ,&nbsp;Katsuhiro Takeda ,&nbsp;Miki Kawada-Matsuo ,&nbsp;Mikihito Kajiya ,&nbsp;Motoyuki Sugai ,&nbsp;Hideki Shiba ,&nbsp;Hitoshi Komatsuzawa","doi":"10.1016/j.job.2025.100679","DOIUrl":"10.1016/j.job.2025.100679","url":null,"abstract":"<div><h3>Introduction</h3><div>Tetracyclines are widely used in dental treatment. Here, we report the genomic information of the tetracycline-resistant <em>Streptococcus mutans</em> strain, HSM45, for the first time.</div></div><div><h3>Methods</h3><div>Susceptibility to tetracycline was determined using the microdilution method. The complete genome sequence of HSM45 was determined and compared with public genome data.</div></div><div><h3>Results</h3><div>HSM45 was resistant to tetracycline. The tetracycline resistance gene <em>tet</em>(M) was carried by Tn<em>916</em>, a conjugative transposon that is widely found in Gram-positive bacteria.</div></div><div><h3>Conclusion</h3><div>This study showed that <em>S. mutans</em> can acquire tetracycline resistance and it can also be a source of horizontal transfer of resistance genes.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 3","pages":"Article 100679"},"PeriodicalIF":2.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144369401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Repetitive/rhythmic masticatory muscle activity under urethane anesthesia in guinea pigs: a descriptive pilot study","authors":"Sho Katsura ,&nbsp;Yutaka Matsuura ,&nbsp;Ayano Katagiri ,&nbsp;Hiroki Toyoda ,&nbsp;Makoto Higashiyama ,&nbsp;Yuji Masuda ,&nbsp;Takafumi Kato","doi":"10.1016/j.job.2025.100673","DOIUrl":"10.1016/j.job.2025.100673","url":null,"abstract":"<div><h3>Objectives</h3><div>Rhythmic/repetitive masticatory muscle activity may occur spontaneously during cyclic alternations in unconscious brain states such as sleep. An experimental model is needed to clarify these underlying mechanisms. This study investigated jaw movements and masticatory muscle activity during cyclic state alternations under urethane anesthesia.</div></div><div><h3>Methods</h3><div>Cortical electroencephalography, electrocardiography, and nasal airflow were recorded simultaneously with jaw movements and jaw muscle electromyography activity in seven urethane-anesthetized male guinea pigs (420–594 g). Cortical brain states were divided into deactivated and activated states according to electroencephalogram (EEG) delta power. The respiratory and heart rates were quantified during the two cortical states. Rhythmic jaw movements (RJMs) were visually scored and the characteristics of masticatory electromyographic bursts were analyzed. Transient changes in cortical, cardiac, and respiratory activities were analyzed in association with RJMs.</div></div><div><h3>Results</h3><div>Cortical activity and respiratory and heart rate variabilities differed significantly between activated and deactivated states. Of 321 RJMs, 290 occurred in clusters under urethane anesthesia; The majority (73.7 %) were scored during the activated state. RJM episodes were associated with alternate lateral jaw excursion, predominantly with masseter muscle activation, and occasionally with tooth-grinding sounds. RJMs were preceded by decreases in EEG delta activity and transient increases in cardiac and respiratory activities.</div></div><div><h3>Conclusions</h3><div>Masticatory muscles may be activated repetitively and rhythmically during cyclic alternations in brain states under urethane anesthesia. Urethane-anesthetized guinea pigs are a potential experimental model for examining the mechanisms underlying the generation of RJMs in unconscious states, such as sleep bruxism.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 3","pages":"Article 100673"},"PeriodicalIF":2.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144192319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical, radiographic, and histological features of buccal bifurcation cysts: A systematic review to aid accurate diagnosis and treatment decisions","authors":"Matheus de Castro Costa ,&nbsp;Rani Kanthan ,&nbsp;Marina Lara de Carli ,&nbsp;Felipe Fornias Sperandio","doi":"10.1016/j.job.2025.100652","DOIUrl":"10.1016/j.job.2025.100652","url":null,"abstract":"<div><h3>Objective</h3><div>This systematic review delves into the nuanced landscape of buccal bifurcation cysts (BBCs), emphasizing their clinical significance amid the diagnostic challenges in oral and maxillofacial pathology. We trace the evolution of BBC classification from historical perspectives to its current status in the World Health Organization's classification system, aiming to equip dental professionals with crucial insights for accurate diagnosis and effective management.</div></div><div><h3>Methods</h3><div>This systematic review (PROSPERO: CRD42023405169) followed PRISMA guidelines to examine the epidemiological characteristics of BBCs. Observational studies were included, while reviews, meta-analyses, and experimental studies were excluded. A comprehensive search across five databases identified eligible studies. Two independent reviewers screened articles, resolving disagreements by consensus or a third reviewer. Data extraction included clinical, histological, and imaging findings. Risk of bias was assessed using Murad's framework for case reports/series and the Newcastle–Ottawa Scale for other study types, with studies rated as low, moderate, or high quality.</div></div><div><h3>Results</h3><div>The information presented here is crucial for preventing past treatment errors associated with BBC. In addition, this review confirms that BBCs predominantly affect the posterior mandible of pediatric patients and exhibit consistent clinical and histopathological features, aiding in their differentiation from similar maxillofacial lesions. Thus, well-informed clinicians should be able to diagnose BBC and make a proper treatment choice after familiarizing themselves with this review, which will ultimately lead to a favorable prognostic outcome and reduced risk of lesion recurrence.</div></div><div><h3>Conclusion</h3><div>This study provides a comprehensive analysis of BBC, aiming to enhance clinical understanding and ultimately improve patient care.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 2","pages":"Article 100652"},"PeriodicalIF":2.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143639740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial effects of great salt lake mineral salts on oral pathogenic bacteria: Implications for oral care","authors":"Inori Inui ,&nbsp;Atsushi Iwatsuki ,&nbsp;Yoshie Yoshioka ,&nbsp;Manabu Habu ,&nbsp;Wataru Ariyoshi ,&nbsp;Ryota Yamasaki","doi":"10.1016/j.job.2025.100633","DOIUrl":"10.1016/j.job.2025.100633","url":null,"abstract":"<div><div>This study investigated the antimicrobial effects of purified natural mineral salts from the Great Salt Lake on oral pathogenic bacteria. Salts rich in sodium, calcium, potassium, and magnesium effectively inhibit the growth and biofilm formation of cariogenic and periodontopathogenic bacteria at lower concentrations than does sodium chloride. These findings suggest the potential applications of these salts in oral care products such as toothpaste and mouthwash.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 2","pages":"Article 100633"},"PeriodicalIF":2.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143548746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alendronate inhibits bone-specific blood vessels in the femoral metaphyses of mice","authors":"Tomoka Hasegawa ,&nbsp;Mako Sakakibara ,&nbsp;Xuanyu Liu ,&nbsp;Hirona Yoshino ,&nbsp;Yan Shi ,&nbsp;Jiaxin Cui ,&nbsp;Mai Haraguchi-Kitakamae ,&nbsp;Weisong Li ,&nbsp;Wang Haoyu ,&nbsp;Tomomaya Yamamoto ,&nbsp;Hotaka Ishizu ,&nbsp;Tamaki Sekiguchi ,&nbsp;Tomohiro Shimizu ,&nbsp;Norio Amizuka","doi":"10.1016/j.job.2025.100655","DOIUrl":"10.1016/j.job.2025.100655","url":null,"abstract":"<div><div>To determine whether alendronate affects vascular endothelial cells and regulates the interactions between blood vessels and osteoblasts, we have examined the femoral metaphyses of alendronate-administered mice. Following administration, the bone-specific blood vessels exhibited significantly reduced luminal diameters and rough luminal surfaces with numerous small protrusions and vesicles. Although the osteoclast distribution remained unchanged in alendronate-treated mice, osteoblasts were inactivated in the metaphyseal regions where blood vessels had shrunk. Additionally, the expression of genes such as <em>Ephb4</em>/<em>Efnb2</em>, which mediate vascular endothelial cell–osteoblast interactions, was diminished. Therefore, alendronate may primarily affect bone-specific blood vessels, thus leading to osteoblast inactivation.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 2","pages":"Article 100655"},"PeriodicalIF":2.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143674736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Letter to the editor, “Mirror-polished ceria-stabilized zirconia/alumina nanocomposite enhances gingival junctional epithelial cell adhesion” [J Oral Biosci, Volume 67, 2025. DOI: 10.1016/j.job.2024.100593]","authors":"Nicholas G. Fischer","doi":"10.1016/j.job.2025.100647","DOIUrl":"10.1016/j.job.2025.100647","url":null,"abstract":"","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 2","pages":"Article 100647"},"PeriodicalIF":2.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143562257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide analyses of susceptibility genes responsible for mandibular prognathism in the Japanese population","authors":"Marie Hoshi-Numahata ,&nbsp;Atsuko Nakanishi-Kimura ,&nbsp;Haruhisa Watanabe ,&nbsp;Mai Nishiura ,&nbsp;Shinnosuke Nishimoto ,&nbsp;Fumi Ueno ,&nbsp;Riyu Koguchi ,&nbsp;Akira Oka ,&nbsp;Yoshiaki Sato ,&nbsp;Takashi S. Kajii ,&nbsp;Tadahiro Iimura","doi":"10.1016/j.job.2025.100670","DOIUrl":"10.1016/j.job.2025.100670","url":null,"abstract":"<div><h3>Background</h3><div>Mandibular prognathism (MP) is a type of malocclusion characterized by an imbalance in the anteroposterior position of the upper and lower jaws. The prevalence of MP in Japan is relatively high, suggesting a unique genetic background in the population.</div></div><div><h3>Highlight</h3><div>Genome-wide analyses identified susceptibility genes responsible for mandibular prognathism in the Japanese population.</div></div><div><h3>Conclusion</h3><div>Identification of the genes associated with malocclusion will pave the way for personalized and precise medicine and contribute to craniofacial biology.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 2","pages":"Article 100670"},"PeriodicalIF":2.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143947569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cyclic compression loading alters osteoarthritis-related gene expression in three-dimensionally cultured human articular chondrocytes via a different mechanism than interleukin-1β induction","authors":"Minami Hikida ,&nbsp;Takashi Kanamoto ,&nbsp;Yoshihito Tachi ,&nbsp;Kosuke Ebina ,&nbsp;Masahiro Nakajima ,&nbsp;Ken Nakata","doi":"10.1016/j.job.2025.100653","DOIUrl":"10.1016/j.job.2025.100653","url":null,"abstract":"<div><h3>Objectives</h3><div>Mechanical and inflammatory stimuli are key factors in the pathophysiology of osteoarthritis (OA). However, the effects of mechanical stimulation on joint tissues and cells at the molecular level and the mechanisms of interaction after stimulation with inflammatory cytokines remains uninvestigated.</div></div><div><h3>Methods</h3><div>Three-dimensional cyclic compression loading (CCL) was applied to human articular chondrocytes, and the expression of OA-related genes was analyzed using reverse transcription quantitative real-time polymerase chain reaction. Additionally, the effects of CCL after the chondrocytes were stimulated with interleukin (IL)-1β were evaluated. A DNA microarray assay was used to compare changes in gene expression after chondrocytes were stimulated with IL-1β and CCL was applied, and to search for pathways that are affected by CCL.</div></div><div><h3>Results</h3><div>CCL of 40 kPa significantly upregulated the expression of IL-8, cyclooxygenase (COX)-2, nerve growth factor, matrix metalloproteinase (MMP)-1, and MMP-3. Transcription of IL-8, COX-2, and MMP-3 was synergistically promoted by CCL and IL-1β. The top 10 pathways enriched in the Kyoto Encyclopedia of Genes and Genomes enrichment analysis of differentially expressed genes were not common in either group, except for the “cytokine-cytokine receptor interaction”. The “tumor necrosis factor signaling pathway” and the “nuclear factor-kappa B signaling pathway” in the IL-1β group and “cell cycle” and the “Hippo signaling pathway” in the CCL group were included.</div></div><div><h3>Conclusions</h3><div>Comprehensive gene expression analysis revealed that CCL-induced changes in gene expression were different to those induced by stimulation with IL-1β. Our results provide new insights into the involvement of mechanical stimulation in the pathogenesis of OA.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 2","pages":"Article 100653"},"PeriodicalIF":2.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143643501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Piezo1 promotes double-directional differentiation from human periodontal ligament progenitor cells","authors":"Yuri Kono ,&nbsp;Hiroshi Kajiya ,&nbsp;Riko Nagano ,&nbsp;Chisato Tominaga ,&nbsp;Hidefumi Maeda ,&nbsp;Tsugumi Fujita ,&nbsp;Sachio Tamaoki","doi":"10.1016/j.job.2025.100651","DOIUrl":"10.1016/j.job.2025.100651","url":null,"abstract":"<div><h3>Objectives</h3><div>Human periodontal ligament (PDL) progenitor cells (hPDLPCs) sense mechanical stress and differentiate into osteoblasts, cementoblasts, and fibroblasts during orthodontic tooth movement. The mechanosensitive ion channel Piezo1 has been known to be present in PDL tissues and is involved in mineralization during bone regeneration. However, the functional role and underlying mechanisms of Piezo1 in osteogenesis and cementogenesis are unknown. We hypothesize that Piezo proteins are expressed in and regulate the differentiation of hPDLPCs.</div></div><div><h3>Methods</h3><div>We examined the effects of Piezo1 activation, by agonist and mechanical stretching, on the expression of osteogenesis- and cementogenesis-related molecules in hPDLPCs using RT-PCR, western blotting, and immunofluorescence methods.</div></div><div><h3>Results</h3><div>hPDLPCs showed calcium influx in Piezo1 and Piezo2, but not in TRPV4 and its channels. In hPDLPCs, the Piezo1 agonist Yoda1 significantly upregulated osteogenesis- and cementogenesis-related molecules through the Ca²⁺/CREB pathway. To investigate the role of Piezo1 in hPDLPC-mediated differentiation, knockout (KO) of Piezo1 in hPDLPCs was generated; significant downregulation of osteogenesis- and cementogenesis-related molecules was observed in KO hPDLPCs. Furthermore, Piezo1 enhanced the mineralization of hPDLPCs.</div></div><div><h3>Conclusions</h3><div>hPDLPCs expressed Piezo1 and Piezo2. Yoda1, Piezo1 agonist, significantly upregulated osteogenesis- and cementogenesis-related molecules through the Ca²⁺/CREB signaling pathway.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 2","pages":"Article 100651"},"PeriodicalIF":2.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143639645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced osteogenic differentiation of human periodontal ligament cells by mature osteoclasts","authors":"Sumit Suamphan ,&nbsp;Anupong Makeudom ,&nbsp;Suttichai Krisanaprakornkit ,&nbsp;Pimphorn Meekhantong ,&nbsp;Ekapong Dechtham ,&nbsp;Chidchanok Leethanakul","doi":"10.1016/j.job.2025.100632","DOIUrl":"10.1016/j.job.2025.100632","url":null,"abstract":"<div><h3>Objective</h3><div>Several <em>in vitro</em> studies have shown that reverse signaling from osteoclasts regulates osteoblast differentiation and mineralization. However, none of these studies have reported the effects of this signaling pathway on periodontal ligament (PDL) cells. Therefore, in this study, we aimed to investigate the interaction between receptor activators of nuclear factor kappa B (RANK) released from mature human osteoclasts and the membranous RANK ligand (RANKL) in human PDL cells.</div></div><div><h3>Methods</h3><div>Multinucleated mature human osteoclasts were differentiated from peripheral blood mononuclear cells upon incubation with recombinant macrophage colony-stimulating factor and RANKL. Mature osteoclasts and human PDL cells were characterized. A mature osteoclast-conditioned medium (OC-CM) was used to induce osteogenic differentiation of PDL cells. Mechanistic analysis of RANK-RANKL reverse signaling were conducted to determine the regulation of osteogenic induction using conditioned medium from mature osteoclasts treated with GW4869 (GW–OC–CM) or PDL cells pretreated with recombinant human osteoprotegerin (OPG).</div></div><div><h3>Results</h3><div>OC-CM significantly upregulated the mRNA expression of osteogenic genes and enhanced the osteogenic differentiation and biomineralization of PDL cells (<em>p</em> &lt; 0.05). GW–OC–CM significantly reduced the expression of osteogenic genes, osteogenic differentiation, and biomineralization in PDL cells (<em>p</em> &lt; 0.05). Similarly, the pretreatment of PDL cells with OPG before OC-CM treatment significantly reduced the osteogenic induction of PDL cells (<em>p</em> &lt; 0.05).</div></div><div><h3>Conclusion</h3><div>Mature osteoclasts can induce osteogenesis in human PDL cells via RANK-RANKL reverse signaling.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 2","pages":"Article 100632"},"PeriodicalIF":2.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143478645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}