Journal of Oral Biosciences最新文献

{"title":"Epigenetic modifier G9a is involved in regulation of mouse tongue development","authors":"Hisashi Ideno ,&nbsp;Kazuhisa Nakashima ,&nbsp;Koichiro Komatsu ,&nbsp;Hiroshi Kimura ,&nbsp;Yoichi Shinkai ,&nbsp;Makoto Tachibana ,&nbsp;Akira Nifuji","doi":"10.1016/j.job.2023.12.007","DOIUrl":"10.1016/j.job.2023.12.007","url":null,"abstract":"<div><h3>Objectives</h3><p>The tongue comprises multiple tissues of different embryonic origins, including pharyngeal arch, somite, and cranial neural crest (CNC). However, its developmental regulatory mechanisms, especially those involving epigenetic modifiers, remain poorly understood. This study examined the roles of the epigenetic modifier G9a in murine tongue development.</p></div><div><h3>Methods</h3><p>We deleted G9a using Sox 9 (SRY-related HMG-box gene 9)-Cre recombinase, which acts in tongue progenitor cells, including CNC-derived cells, to generate G9a conditional knockout (cKO) mice. Histochemical and immunohistochemical analyses were conducted on sections prepared from tongue tissues of control and cKO mice.</p></div><div><h3>Results</h3><p>Cre-dependent LacZ reporter mice, generated by crossing Rosa-LacZ mice with sox9-Cre mice, revealed Cre recombinase activity in the mucosal epithelium and tongue connective tissue of the embryonic tongue. Tongue volume was significantly reduced on embryonic day 17.5 (E17.5) and postnatal day 0 (P0) in cKO mice. Histological sections showed that the lingual mucosal epithelium was thinner in cKO mice. Reduced G9a levels were accompanied by decreased levels of a G9a substrate, dimethylated lysine 9 in histone H3, in the embryonic tongue. BrdU injection at E16.5 revealed reduced numbers of BrdU-positive cells in the mucosal epithelium and underlying connective tissue at E17.5 in cKO mice, indicating suppression of cell proliferation in both tissues. Investigation of keratin 5 and 8 protein localization showed significantly suppressed expression in the lingual mucosal epithelium in cKO mice.</p></div><div><h3>Conclusions</h3><p>G9a is required for proper proliferation and differentiation of sox9-expressing tongue progenitor cells and is thereby involved in tongue development.</p></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"66 1","pages":"Pages 35-40"},"PeriodicalIF":2.4,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1349007923001986/pdfft?md5=2f81a637b48fd62554c99f81af5f71a5&pid=1-s2.0-S1349007923001986-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139014083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Putative role of endothelin receptor B in the development and maintenance of taste buds within the circumvallate papillae","authors":"Jong-Min Lee,&nbsp;Han-Sung Jung","doi":"10.1016/j.job.2024.01.005","DOIUrl":"10.1016/j.job.2024.01.005","url":null,"abstract":"<div><p>This study aimed to achieve a better understanding of taste receptor cell development relative to endothelin receptor B (ETB) in circumvallate papillae (CVP). ETB localization was assessed by immunohistochemistry during tongue development of the mouse. Co-localization of ETB with taste receptor type III cell marker, Synaptosomal-Associated Protein 25 kDa (SNAP25), was evident in both the developing and adult CVP. ETB was strongly localized in the stromal core region. As development progressed, ETB became localized in the CVP mesenchyme and partially in the epithelium. ETB and SNAP25 co-localization indicates that ETB may regulate innervation from the CVP mesenchyme to taste buds.</p></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"66 1","pages":"Pages 249-252"},"PeriodicalIF":2.4,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1349007924000057/pdfft?md5=d7239c8fd709b5c61b6baa22445c4080&pid=1-s2.0-S1349007924000057-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139467323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rab11 suppresses head and neck carcinoma by regulating EGFR and EpCAM exosome secretion","authors":"Kunihiro Yoshida ,&nbsp;Kaung Htike ,&nbsp;Takanori Eguchi ,&nbsp;Hotaka Kawai ,&nbsp;Htoo Shwe Eain ,&nbsp;Manh Tien Tran ,&nbsp;Chiharu Sogawa ,&nbsp;Koki Umemori ,&nbsp;Tatsuo Ogawa ,&nbsp;Hideka Kanemoto ,&nbsp;Kisho Ono ,&nbsp;Hitoshi Nagatsuka ,&nbsp;Akira Sasaki ,&nbsp;Soichiro Ibaragi ,&nbsp;Kuniaki Okamoto","doi":"10.1016/j.job.2023.11.007","DOIUrl":"10.1016/j.job.2023.11.007","url":null,"abstract":"<div><h3>Objectives</h3><p>Rab11(Rab11a and Rab11b) localizes primarily along recycling endosomes in cells and is involved in various intracellular trafficking processes, including membrane receptor recycling and secretion of exosomes or small extracellular vesicles (EVs). Although Rab11 is closely associated with the progression and metastasis of various cancer types, little is known about Rab11’ role in head and neck squamous cell carcinoma (HNSCC). In this study, we investigated the roles of Rab11a and Rab11b in HNSCC.</p></div><div><h3>Methods</h3><p>The clinical significance of Rab11 expression in HNSCC was investigated using a public database and tissue microarray analysis. Stable cell lines with loss and gain of Rab11a or Rab11b were originally established to investigate their roles in the proliferative, migratory, and invasive capabilities of HNSCC cells.</p></div><div><h3>Results</h3><p>Database analysis revealed a significant association between Rab11b mRNA expression and a favorable patient survival rate in HNSCC. Tissue microarray analysis revealed that Rab11b expression was the highest in normal tissues and gradually decreased across the stages of HNSCC progression. Overexpression of Rab11a or Rab11b resulted in a decrease in epidermal growth factor receptor (EGFR), Epithelial cell adhesion molecule (EpCAM) exosome secretion, and the migratory and invasive potential of HNSCC cells. The knockdown of Rab11a or Rab11b increased EpCAM/CD9 exosome secretion in addition to the migratory and invasive potential of HNSCC cells.</p></div><div><h3>Conclusions</h3><p>Rab11 suppresses HNSCC by regulating EGFR recycling and EpCAM exosome secretion in HNSCC cells. Our results indicate that Rab11b is a superior prognostic indicator of HNSCC and holds promise for developing novel therapeutic strategies.</p></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"66 1","pages":"Pages 205-216"},"PeriodicalIF":2.4,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1349007923001883/pdfft?md5=01b32f478220a18f2fbbbbd0fb4ef775&pid=1-s2.0-S1349007923001883-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138811857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the effect of butyric acid, a metabolite from periodontopathic bacteria, on primary human melanocytes: An in vitro study","authors":"Shilpi Goenka","doi":"10.1016/j.job.2024.01.002","DOIUrl":"10.1016/j.job.2024.01.002","url":null,"abstract":"<div><p>Effects of butyric acid, a bacterial metabolite implicated in periodontitis progression, have never been examined on oral melanocytes. Herein, primary human epidermal melanocytes were used as a model for oral melanocytes. Results show the adverse effects of butyric acid (sodium butyrate; NaB) on them, which comprise marked cytotoxicity at higher concentrations (&gt;1 mM) and robust differentiation at lower nontoxic concentrations. NaB did not alter MITF protein levels; however, it stimulated tyrosinase protein synthesis and inhibited tyrosinase activity, with no changes in cellular melanin. NaB did not affect oxidative stress, although it induced significant levels of the pro-inflammatory cytokine IL-6.</p></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"66 1","pages":"Pages 253-259"},"PeriodicalIF":2.4,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1349007924000021/pdfft?md5=08d178d8315fe4026617ac417c868720&pid=1-s2.0-S1349007924000021-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139433101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological differences between the first and second maxillary premolar crowns: A three-dimensional surface homologous modeling analysis","authors":"Julie Miyazaki ,&nbsp;Shintaro Kondo ,&nbsp;Toyohisa Tanijiri ,&nbsp;Shinichi Negishi","doi":"10.1016/j.job.2024.01.010","DOIUrl":"10.1016/j.job.2024.01.010","url":null,"abstract":"<div><h3>Objectives</h3><p>The current study used a three-dimensional (3D) surface homologous modeling to analyze the structure of maxillary first premolar (P¹) and second premolar (P²) crowns, to identify any morphological differences between them, particularly in their cuspal structures.</p></div><div><h3>Methods</h3><p>The study sample comprised 27 male elementary and junior high school students from Chiba Prefecture, Japan. Plaster casts were collected and the 3D coordinates were used to measure the crown structures. Thereafter, principal component (PC) analysis was carried out using the 3D coordinates of the homologous models, containing 4498 anatomical data points, including 9 landmarks.</p></div><div><h3>Results</h3><p>The findings indicated that P¹ was significantly larger than P², despite both teeth exhibiting similar intercuspal distances. The homologous model analysis revealed that 61.5 % of the total variance could be explained up to the fourth PC. Overall size and shape in the mesiodistal and buccolingual directions were estimated using PC1 and PC2, respectively. Both components highlighted a shape factor, indicating that the buccal cusp was more well-developed than the lingual cusp in P¹ compared to P².</p></div><div><h3>Conclusions</h3><p>The variations in the size of the mesial and distal premolar teeth and the relationships between the cusps in the completed tooth crowns can be explained using molecular biology developmental models.</p></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"66 1","pages":"Pages 20-25"},"PeriodicalIF":2.4,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1349007924000100/pdfft?md5=26ae6c6648a1da20e7de50bd85536f97&pid=1-s2.0-S1349007924000100-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139571777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of macrophages in trigeminal ganglia in ectopic orofacial pain associated with pulpitis","authors":"Miki Sunaga,&nbsp;Yoshiyuki Tsuboi,&nbsp;Akihiro Kaizu,&nbsp;Masamichi Shinoda","doi":"10.1016/j.job.2024.02.001","DOIUrl":"10.1016/j.job.2024.02.001","url":null,"abstract":"<div><h3>Objectives</h3><p>This study aimed to elucidate the role of macrophages in the trigeminal ganglia (TG) in developing pulpitis-associated ectopic orofacial pain.</p></div><div><h3>Methods</h3><p>Rats underwent maxillary pulp exposure, and Fluoro-Gold (FG) was administered in the ipsilateral whisker pad (WP). Head withdrawal threshold (HWT) upon mechanical stimulation of the WP was recorded, and liposomal clodronate clophosome-A (LCCA; macrophage depletion agent) was administered to the TG at three and four days after pulp exposure. Immunohistochemically, TG sections were stained with anti-Iba1 (a macrophage marker) and anti-Nav1.7 antibodies.</p></div><div><h3>Results</h3><p>Pulp exposure decreased HWT and increased the number of Iba1-IR cells near FG-labelled TG neurons. LCCA inhibited the decrease in HWT and stopped the increase of FG-labelled Nav1.7-IR TG neurons in the pulpitis group.</p></div><div><h3>Conclusions</h3><p>Activation of macrophages by pulpitis induces the overexpression of Nav1.7 in TG neurons receiving inputs from WP, resulting in pulpitis-induced ectopic facial mechanical allodynia.</p></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"66 1","pages":"Pages 145-150"},"PeriodicalIF":2.4,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1349007924000148/pdfft?md5=f3eb16b7aae5604655707bdea0c039be&pid=1-s2.0-S1349007924000148-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139717884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of IgA nephropathy on the progression of pulpitis and apical periodontitis in HIGA mice","authors":"Reona Hayashi ,&nbsp;Shiori Yamazaki ,&nbsp;Noriko Mutoh ,&nbsp;Tatsuo Hashimoto ,&nbsp;Hayato Ohshima ,&nbsp;Nobuyuki Tani-Ishii","doi":"10.1016/j.job.2023.11.003","DOIUrl":"10.1016/j.job.2023.11.003","url":null,"abstract":"<div><h3>Objectives</h3><p>Immunoglobulin (Ig)A nephropathy has been associated with oral infections such as periodontitis, but its pathogenesis is not fully understood; no treatments exist. This study analyzes the influence of IgA nephropathy, an autoimmune disease, on the pathogenesis of pulpitis and apical periodontitis.</p></div><div><h3>Methods</h3><p>Two groups of mice were used in pulp infection experiments: high serum IgA nephropathy model mice (HIGA) and control mice (BALB/c). Histologic analyses of the pulp and apical periodontal tissues were performed on days 3, 5, 7, 14, and 28 following oral bacterial infection. The dynamics of odontoblasts, apoptotic cells, and IgA expression were analyzed using anti-Nestin, TUNEL, and anti-IgA staining, respectively.</p></div><div><h3>Results</h3><p>Inflammatory cells infiltrated the exposed pulp at day three in both groups and by 14 days, these cells had infiltrated from the pulp to the apical periodontal tissue. The area of necrotic pulp tissue increased significantly in the control group at seven days. Odontoblasts decreased from day three onwards and disappeared by 28 days in both groups. The number of apoptotic cells in the pulp and apical periodontal tissues was significantly higher in the experimental group at day 28. The experimental group exhibited a significant increase in IgA production in the pulp after 14 days. Bone resorption in the apical periodontal tissue was significantly decreased in the experimental group at day 28.</p></div><div><h3>Conclusions</h3><p>The results of this study suggest that IgA nephropathy may modulate the inflammatory response and sustain long-term biological defense responses in pulpitis and apical periodontitis in HIGA mice.</p></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"66 1","pages":"Pages 98-104"},"PeriodicalIF":2.4,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1349007923001846/pdfft?md5=398eac2e39bd8893e2d4caac5fc0ad9d&pid=1-s2.0-S1349007923001846-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138048167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome and metabolome analyses of Streptococcus gordonii DL1 under acidic conditions","authors":"Naoto Hayashida ,&nbsp;Yumiko Urano-Tashiro ,&nbsp;Tetsuro Horie ,&nbsp;Keitarou Saiki ,&nbsp;Yuki Yamanaka ,&nbsp;Yukihiro Takahashi","doi":"10.1016/j.job.2023.12.005","DOIUrl":"10.1016/j.job.2023.12.005","url":null,"abstract":"<div><h3>Objectives</h3><p><em>Streptococcus gordonii</em> is associated with the formation of biofilms, especially those that comprise dental plaque. Notably, <em>S. gordonii</em> DL1 causes infective endocarditis (IE). Colonization of this bacterium requires a mechanism that can tolerate a drop in environmental pH by producing acid via its own sugar metabolism. The ability to survive acidic environmental conditions might allow the bacterium to establish vegetative colonization even in the endocardium due to inflammation-induced lowering of pH, increasing the risk of IE. At present, the mechanism by which <em>S. gordonii</em> DL1 survives under acidic conditions is not thoroughly elucidated. The present study was thus conducted to elucidate the mechanism(s) by which <em>S. gordonii</em> DL1 survives under acidic conditions.</p></div><div><h3>Methods</h3><p>We analyzed dynamic changes in gene transcription and intracellular metabolites in <em>S. gordonii</em> DL1 exposed to acidic conditions, using transcriptome and metabolome analyses.</p></div><div><h3>Results</h3><p>Transcriptome analysis revealed upregulation of genes involved in heat shock response and glycolysis, and down regulation of genes involved in phosphotransferase systems and biosynthesis of amino acids. The most upregulated genes were a beta-strand repeat protein of unknown function (<em>SGO_RS06325</em>), followed by copper-translocating P-type ATPase (<em>SGO_RS09470</em>) and malic enzyme (<em>SGO_RS01850</em>). The latter two of these contribute to cytoplasmic alkalinization. <em>S. gordonii</em> mutant strains lacking each of these genes showed significantly reduced survival under acidic conditions. Metabolome analysis revealed that cytoplasmic levels of several amino acids were reduced.</p></div><div><h3>Conclusions</h3><p><em>S. gordonii</em> survives the acidic conditions by recovering the acidic cytoplasm using the various activities, which are regulated at the transcriptional level.</p></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"66 1","pages":"Pages 112-118"},"PeriodicalIF":2.4,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1349007923001962/pdfft?md5=d725b74f29091a24ad2da05c0a2726ab&pid=1-s2.0-S1349007923001962-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138886213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Globo-series Gb4 activates ERK and promotes the proliferation of osteoblasts","authors":"Hanami Kato ,&nbsp;Mayu Nagao ,&nbsp;Koichi Furukawa ,&nbsp;Yoshitaka Mishima ,&nbsp;Shota Ichikawa ,&nbsp;Takuma Sato ,&nbsp;Ken Miyazawa ,&nbsp;Kazunori Hamamura","doi":"10.1016/j.job.2023.10.004","DOIUrl":"10.1016/j.job.2023.10.004","url":null,"abstract":"<div><h3>Objectives</h3><p>Globo-series Gb4 (globoside) is involved in the immune system and disease pathogenesis. We recently reported that systemic Gb4 deficiency in mice led to decreased bone formation due to a reduction in osteoblast number. However, it remains unclear whether Gb4 expressed in osteoblasts promotes their proliferation. Therefore, we investigated the role of Gb4 in osteoblast proliferation <em>in vitro</em>.</p></div><div><h3>Methods</h3><p>We examined osteoblast proliferation in Gb3 synthase knockout mice lacking Gb4. We investigated the effects of Gb4 synthase knockdown in the mouse osteoblast cell line MC3T3-E1 on its proliferation. Furthermore, we administered Gb4 to MC3T3-E1 cells in which Gb4 was suppressed by a glucosylceramide synthase (GCS) inhibitor and evaluated its effects on their proliferation. To elucidate the mechanisms by which Gb4 promotes osteoblast proliferation, the phosphorylated extracellular signal-regulated kinases 1 and 2 (ERK1/2) levels were measured in MC3T3-E1 cells.</p></div><div><h3>Results</h3><p>Osteoblast proliferation was lower in Gb3 synthase knockout mice lacking Gb4 than in wild-type mice. Proliferation was inhibited by Gb4 synthase knockdown in MC3T3-E1 cells. Furthermore, the administration of Gb4 to MC3T3-E1 cells, in which a GCS inhibitor suppressed Gb4, promoted their proliferation. Moreover, it increased the phosphorylated ERK1/2 levels in MC3T3-E1 cells.</p></div><div><h3>Conclusions</h3><p>Our results suggest that Gb4 expressed in osteoblasts promotes their proliferation through ERK1/2 activation.</p></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"66 1","pages":"Pages 41-48"},"PeriodicalIF":2.4,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1349007923001792/pdfft?md5=9a7f0f23e008ef2aec3886072bb8335e&pid=1-s2.0-S1349007923001792-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71522910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ser252Trp mutation in fibroblast growth factor receptor 2 promotes branching morphogenesis in mouse salivary glands","authors":"Daiki Iwata ,&nbsp;Kaori Kometani-Gunjigake ,&nbsp;Kayoko Nakao-Kuroishi ,&nbsp;Masahiro Mizuhara ,&nbsp;Mitsushiro Nakatomi ,&nbsp;Keiji Moriyama ,&nbsp;Kentaro Ono ,&nbsp;Tatsuo Kawamoto","doi":"10.1016/j.job.2024.01.001","DOIUrl":"10.1016/j.job.2024.01.001","url":null,"abstract":"<div><h3>Objectives</h3><p>The purpose of this study was to perform morphological and immunohistochemical (IHC) analysis of the submandibular glands (SMGs) in early development in Apert syndrome model mice (Ap mice).</p></div><div><h3>Methods</h3><p><em>ACTB-Cre</em> homozygous mice were mated with fibroblast growth factor receptor 2 (<em>Fgfr2</em>^{<em>+/Neo-S252W</em>}) mice; <em>ACTB-Cre</em> heterozygous mice (ACTB-Cre mice) at embryonic day (E) 13.5 served as the control group, and <em>Fgfr2</em>^{<em>+/S252W</em>} mice (Ap mice) served as the experimental group. Hematoxylin and eosin (H&amp;E) staining was performed on SMGs; Total SMG area and epithelial area were determined, and the epithelial occupancy ratio was calculated. Immunostaining was performed to assess the localization of FGF signaling-related proteins. Next, bromodeoxyuridine (BrdU)-positive cells were evaluated to assess cell proliferation. Finally, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining was performed to assess apoptosis in SMGs.</p></div><div><h3>Results</h3><p>The epithelial occupancy ratio was significantly higher in SMGs of Ap mice compared with that in SMGs of controls. FGF7 and bone morphogenetic protein 4 (BMP4) exhibited different localizations in SMGs of Ap mice compared with SMGs of controls. Cell proliferation was higher in SMGs of Ap mice compared with that of controls; however, apoptosis did not different significantly between the two groups.</p></div><div><h3>Conclusion</h3><p>Our results suggest that enhanced FGF signaling conferred by missense mutations in FGFR2 promotes branching morphogenesis in SMGs of Ap mice.</p></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"66 1","pages":"Pages 90-97"},"PeriodicalIF":2.4,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S134900792400001X/pdfft?md5=7ccae4cc6399f8f0219db40cc88dbd09&pid=1-s2.0-S134900792400001X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139513532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}