IF 1.7

GERMS Pub Date : 2024-03-31 eCollection Date: 2024-03-01 DOI: 10.18683/germs.2024.1413

Amira M Sultan, Noha Mostafa Mahmoud

{"title":"Detection of resistance integrons among biofilm and non-biofilm producing clinical isolates of Pseudomonas aeruginosa.","authors":"Amira M Sultan, Noha Mostafa Mahmoud","doi":"10.18683/germs.2024.1413","DOIUrl":"10.18683/germs.2024.1413","url":null,"abstract":"Introduction: Integrons are genetic systems that may confer antibiotic resistance to Pseudomonas aeruginosa. Biofilm formation can facilitate gene exchange and can accelerate the development of antibiotic resistance. The aim of this work was to assess the distribution of resistance integrons including class 1, 2 and 3 among biofilm- and non-biofilm producing clinical strains of P. aeruginosa. We also aimed to investigate the relationship between the existence of these integrons and the isolates' resistance patterns.Methods: Specimens were obtained from patients showing evidence of infection. P. aeruginosa isolates were identified using conventional techniques, while disk diffusion test was used to detect their antimicrobial susceptibilities. Biofilm formation was detected by the tissue culture plate technique, while classes of integrons were detected by polymerase chain reaction.Results: Out of 106 P. aeruginosa isolates, 55.7% were class 1 integron-positive while 19.8% were class 2 integron-positive. However, class 3 integrons were not detected. Significant associations were found between class 1 integrons and resistance toward amikacin, gentamicin, cefepime, ceftazidime and ciprofloxacin. Class 2 integrons were associated with amikacin, ceftazidime and cefepime resistance. Of pseudomonal isolates, 61.3% were biofilm producing. Biofilm production was associated significantly with the existence of class 1 integrons (p<0.001) and class 2 integrons (p=0.039).Conclusions: About two thirds of isolated strains harbored resistance integrons, which emphasized their significance in our locality. The frequencies of class 1 and 2 integrons were significantly higher among biofilm forming isolates. Ongoing surveillance and infection control strategies are necessary to limit spread of integrons.","PeriodicalId":45107,"journal":{"name":"GERMS","volume":"14 1","pages":"11-19"},"PeriodicalIF":1.7,"publicationDate":"2024-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11333839/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142019030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epidemiology of Pseudomonas aeruginosa in diabetic foot infections: a global systematic review and meta-analysis. 糖尿病足感染中铜绿假单胞菌的流行病学：全球系统回顾和荟萃分析。

IF 2

GERMS Pub Date : 2023-12-31 eCollection Date: 2023-12-01 DOI: 10.18683/germs.2023.1406

Mitra Garousi, Sina MonazamiTabar, Hosein Mirazi, Zahra Farrokhi, Azad Khaledi, Ali Shakerimoghaddam

{"title":"Epidemiology of Pseudomonas aeruginosa in diabetic foot infections: a global systematic review and meta-analysis.","authors":"Mitra Garousi, Sina MonazamiTabar, Hosein Mirazi, Zahra Farrokhi, Azad Khaledi, Ali Shakerimoghaddam","doi":"10.18683/germs.2023.1406","DOIUrl":"10.18683/germs.2023.1406","url":null,"abstract":"Pseudomonas aeruginosa is one of the most common causes of diabetic foot infection globally. This study aimed to determine the global distribution of P. aeruginosa isolated from diabetic foot ulcer infection. PRISMA procedure was used to perform the current systematic review and meta-analysis. The Web of Science, MEDLINE/PubMed, Scopus, and other databases were searched for studies published in English from 2000 to 2022. Data was analyzed using the Comprehensive Meta-Analysis software (CMA). Keywords and MESH phrases included Pseudomonas aeruginosa, diabetic foot ulcer, P. aeruginosa, and diabetic foot infection. As a result of this review, 16.6% of diabetic foot wound infections were caused by P. aeruginosa. About 37.9% of strains were multidrug resistant (MDR). P. aeruginosa infection rates in diabetic foot ulcers ranged from 0.5 to 100% globally. In total, the prevalence rates of P. aeruginosa in diabetic foot ulcer infection from Asia, Africa, and Western countries were reported at 18.5%, 16.3%, and 11.1%, respectively. Data have shown that the prevalence of P. aeruginosa, particularly MDR strains, isolated from diabetic foot ulcer infection was relatively high; inherent resistance to antibiotics is also high; the wound either does not heal or if it does, it will be delayed. Therefore, timely treatment is essential.","PeriodicalId":45107,"journal":{"name":"GERMS","volume":"13 4","pages":"362-372"},"PeriodicalIF":2.0,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10866166/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139742279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hydropneumothorax as a complication of necrotizing pneumonia. 作为坏死性肺炎并发症的水肺气胸。

IF 2

GERMS Pub Date : 2023-12-31 eCollection Date: 2023-12-01 DOI: 10.18683/germs.2023.1402

Andria Papazachariou, Eleni Papadokostaki, Despoina Kypraiou, Vironas Malikides, Ioanna Papakitsou, Theodosios D Filippatos, Petros Ioannou, Diamantis P Kofteridis

引用次数: 0

Acinetobacter baumannii: a known pathogen, a new problem. 鲍曼不动杆菌：已知病原体，新问题。

IF 2

GERMS Pub Date : 2023-12-31 eCollection Date: 2023-12-01 DOI: 10.18683/germs.2023.1408

Ricardo Campos Monteiro, Rogerio Caldeira Rodrigues Malta, Geovana Lacerda Rodrigues, Gustavo Luis de Paiva Anciens Ramos, Janaína Dos Santos Nascimento

引用次数: 0

Effect of dexamethasone and tenoxicam on the virulence activities of different Pseudomonas aeruginosa clinical isolates. 地塞米松和替诺昔康对不同铜绿假单胞菌临床分离株毒力活性的影响

IF 2

GERMS Pub Date : 2023-12-31 eCollection Date: 2023-12-01 DOI: 10.18683/germs.2023.1401

Heba E AboElfarh, El-Sayed E Habib, Mohamed M A El-Sokkary

{"title":"Effect of dexamethasone and tenoxicam on the virulence activities of different Pseudomonas aeruginosa clinical isolates.","authors":"Heba E AboElfarh, El-Sayed E Habib, Mohamed M A El-Sokkary","doi":"10.18683/germs.2023.1401","DOIUrl":"10.18683/germs.2023.1401","url":null,"abstract":"Introduction: This study aimed to examine the effect of commonly used non-antibiotic drugs (dexamethasone and tenoxicam), on treatment of Pseudomonas aeruginosa infections, antibiotic resistance and virulence in this pathogen.Methods: Four antibiotics (gentamicin, cefepime, ciprofloxacin and meropenem) were investigated. The proteolysis and hemolysis were selected as virulence factors for investigation. In this work, we selected the following final concentrations: dexamethasone (0.0052 μg/mL) and tenoxicam (2.7 μg/mL) to be used in combination with antibiotics or alone for investigation of their effects on antibiotic resistance and virulence in P. aeruginosa isolates.Results: The drugs either increased or decreased antibiotic resistance in only 0-3 isolates, which indicates that the investigated drugs did not significantly affect the antibiotic resistance. Interestingly, our study demonstrated that both dexamethasone and tenoxicam increased the hemolytic activity of the investigated isolates. On the other hand, our results indicated that no overall final increasing or decreasing effect could be observed for dexamethasone on the proteolytic activity, while tenoxicam increased the proteolytic activity of the investigated isolates. Interestingly, by real-time PCR dexamethasone has shown significant down-regulation of virulence genes namely algD, plcH and toxA, apparently, in case of combination with ciprofloxacin and with gentamicin in one isolate. However, a negative influence was observed in another isolate. Unfortunately, in the case of tenoxicam the only positive effect was observed in the combination with gentamicin in one isolate.Conclusions: Resistance of P. aeruginosa against gentamicin and ciprofloxacin may be affected by combining these antibiotics with dexamethasone or tenoxicam.","PeriodicalId":45107,"journal":{"name":"GERMS","volume":"13 4","pages":"321-331"},"PeriodicalIF":2.0,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10866164/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139742224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Changing clinical patterns and ear-nose-throat complications of seasonal viral respiratory tract infections. 季节性病毒性呼吸道感染不断变化的临床模式和耳鼻喉并发症。

IF 2

GERMS Pub Date : 2023-12-31 eCollection Date: 2023-12-01 DOI: 10.18683/germs.2023.1399

Mihai Săndulescu, Oana Săndulescu

引用次数: 0

Fosfomycin: A potential oral option for treatment of urinary tract infections in Sri Lanka in the context of high antibiotic resistance. 磷霉素：在斯里兰卡抗生素耐药性高发的情况下，治疗尿路感染的一种潜在口服选择。

IF 2

GERMS Pub Date : 2023-12-31 eCollection Date: 2023-12-01 DOI: 10.18683/germs.2023.1400

Nishadi Jayathilaka, Tharushi Pathirana, Chathurika Kumari, Varuna Navaratne, Samanmalee Gunasekara, Dilini Nakkawita, Thamarasi Senaratne

{"title":"Fosfomycin: A potential oral option for treatment of urinary tract infections in Sri Lanka in the context of high antibiotic resistance.","authors":"Nishadi Jayathilaka, Tharushi Pathirana, Chathurika Kumari, Varuna Navaratne, Samanmalee Gunasekara, Dilini Nakkawita, Thamarasi Senaratne","doi":"10.18683/germs.2023.1400","DOIUrl":"10.18683/germs.2023.1400","url":null,"abstract":"Introduction: Fosfomycin is an effective treatment for urinary tract infections (UTIs). It is not currently used in Sri Lanka to treat UTIs. Hence, this study was conducted to assess the fosfomycin susceptibility for E. coli in urinary isolates, with an aim to find the usability of fosfomycin in the context of high antibiotic resistance.Methods: E. coli isolates were identified by the colony appearance and by performing biochemical tests for the urinary coliform isolates collected from two different hospitals in Western Province Sri Lanka, during the period of November 2021 to February 2022. Susceptibility to fosfomycin 200 μg disc was performed following the Clinical Laboratory Standard Institute (CLSI) disc diffusion method.Results: A total of 101 E. coli isolates from both oncology patients (52.5%) and non-oncology patients (47.5%) were identified and included in the study. The study sample showed majority of females (63.3%). Ampicillin showed the highest resistance rate (72.2%) while fosfomycin was the only antibiotic that showed 100% in vitro susceptibility to all the tested clinical isolates. The overall presence of multidrug-resistant (MDR) and carbapenem-resistant (CR) E. coli were 47.5% and 9.9% respectively.Conclusions: Fosfomycin is a potential antibiotic option especially for MDR and CR organisms, with 100% in vitro susceptibility. Further studies involving multiple centers, with larger sample size and clinical efficacy studies would be important to assess the potential use of fosfomycin especially for the treatment of UTI-causing MDR and CR organisms.","PeriodicalId":45107,"journal":{"name":"GERMS","volume":"13 4","pages":"314-320"},"PeriodicalIF":2.0,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10866161/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139742281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The analysis of saliva as screening in patients with COVID-like symptoms. 分析唾液，筛查 COVID 类症状患者。

IF 2

GERMS Pub Date : 2023-12-31 eCollection Date: 2023-12-01 DOI: 10.18683/germs.2023.1410

Giorgio Novelli, Mattia Moretti, Maria Costanza Meazzini, Davide Paolo Bernasconi, Sergio Maria Ivano Malandrin, Monica Raggi, Cristina Maria Angela Cassé, Luca Arturo Pavesi, Davide Sozzi

引用次数: 0

Role of gender in hesitancy toward the COVID-19 vaccine beyond medical students: Reply to Rujittika Mungmunpuntipantip and Viroj Wiwanitkit. 性别在医学生对 COVID-19 疫苗犹豫不决中的作用：回复 Rujittika Mungmunpuntipantip 和 Viroj Wiwanitkit。

IF 2

GERMS Pub Date : 2023-12-31 eCollection Date: 2023-12-01 DOI: 10.18683/germs.2023.1409

Aris P Agouridis, Spyridon A Karageorgos, Constantinos Tsioutis

引用次数: 0

Bacterial species associated with peri-implant disease - a literature review. 与种植体周围疾病相关的细菌种类--文献综述。

IF 2

GERMS Pub Date : 2023-12-31 eCollection Date: 2023-12-01 DOI: 10.18683/germs.2023.1405

Mihai Săndulescu, Valentin Daniel Sîrbu, Ion Alexandru Popovici

引用次数: 0

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