Journal of Oral and Maxillofacial Surgery Medicine and Pathology最新文献

{"title":"Exosome-derived miR-1290 in blood as a predictive biomarker for occult neck metastasis in patients with cT1-2N0 tongue cancer","authors":"Junki Inoue ,&nbsp;Keisuke Yamana ,&nbsp;Ryoji Yoshida ,&nbsp;Yuki Seki ,&nbsp;Kosuke Shinohara ,&nbsp;Akiyuki Hirosue ,&nbsp;Kenta Kawahara ,&nbsp;Masafumi Nakamoto ,&nbsp;Masatoshi Hirayama ,&nbsp;Nozomu Takahashi ,&nbsp;Hideki Nakayama","doi":"10.1016/j.ajoms.2024.12.023","DOIUrl":"10.1016/j.ajoms.2024.12.023","url":null,"abstract":"<div><h3>Objective</h3><div>This study aimed to identify and evaluate the diagnostic potential of exosome-derived microRNAs (miRNAs) for the early detection of occult neck metastasis (ONM) in tongue squamous cell carcinoma (TSCC), a significant postoperative challenge.</div></div><div><h3>Methods</h3><div>Serum samples collected from 12 patients pre-treatment were divided into two groups: Cohort A: patients with cT1–2N0 TSCC who underwent transoral partial glossectomy alone and occurred (n = 3) or did not occur ONM (n = 3); Cohort B: patients with cN0 TSCC who underwent elective neck dissection (END) and diagnosed (n = 3) or did not diagnose pathological lymph node metastasis (n = 3). Exosomal miRNA was extracted for miRNA microarray analysis to identify candidate miRNAs, and the diagnostic potential was assessed using preoperative serum samples in 45 patients with cT1–2N0 TSCC who underwent transoral partial glossectomy. Real-time quantitative RT-PCR validated the expression of candidate miRNAs. Univariate analysis was used to evaluate the association between miR-1290 expression and clinicopathological factors. The log-rank test and multivariate Cox proportional hazards regression analysis was used to investigate clinicopathological risk factors, including miRNAs.</div></div><div><h3>Results</h3><div>Microarray and ROC analyses identified miR-1290 as a potential ONM biomarker. Univariate analysis indicated a significant correlation between miR-1290 expression and the clinical depth of invasion. Although miR-1290 was significantly associated with ONM occurrence in the log-rank test, the multivariate analysis with established ONM predictors did not reach statistical significance.</div></div><div><h3>Conclusions</h3><div>Exosome-derived miR-1290 may serve as a potential biomarker for ONM in patients with early stage TSCC, aiding END. However, larger prospective observational studies are needed to confirm this hypothesis.</div></div>","PeriodicalId":45034,"journal":{"name":"Journal of Oral and Maxillofacial Surgery Medicine and Pathology","volume":"37 4","pages":"Pages 631-638"},"PeriodicalIF":0.4,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144221569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Considerations on surgical ciliated cyst, an entity recognized by the World Health Organization","authors":"Daiana Cristina Pereira Santana,&nbsp;Águida Cristina Gomes Henriques","doi":"10.1016/j.ajoms.2024.12.025","DOIUrl":"10.1016/j.ajoms.2024.12.025","url":null,"abstract":"","PeriodicalId":45034,"journal":{"name":"Journal of Oral and Maxillofacial Surgery Medicine and Pathology","volume":"37 4","pages":"Pages 854-855"},"PeriodicalIF":0.4,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144221563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-term stability of mandibular transverse width after symphyseal fracture associated with bilateral condylar fracture: A case series","authors":"Keigo Maeda,&nbsp;Shinsuke Yamamoto,&nbsp;Naoki Taniike","doi":"10.1016/j.ajoms.2025.01.002","DOIUrl":"10.1016/j.ajoms.2025.01.002","url":null,"abstract":"<div><h3>Objective</h3><div>This study aimed to evaluate the long-term stability of the transverse width of the mandible after surgery for symphyseal region fractures associated with bilateral condylar fractures.</div></div><div><h3>Methods</h3><div>This case series study reviewed data of patients with mandibular symphyseal region fracture and bilateral condylar fracture who underwent open reduction and internal fixation (ORIF) of symphyseal region fracture; either closed treatment or ORIF for condylar fracture were included. Changes in intergonial and intercondylar dimensions from preoperative status to 6 months after ORIF were analysed. The amount of changes was calculated using surface-based superimposition with ProPlanCMF (Materialize, Belgium).</div></div><div><h3>Results</h3><div>Thirteen patients were included in this study. The mean change between the intergonial width preoperatively and intergonial width a few days postoperatively was −3.3 mm (SD: 5.8 mm, range: –12.5–5.2 mm) and that between the first few postoperative days and 6 months postoperative was −0.58 mm (SD: 1.2 mm, range: −3.0–0.9 mm). The mean change in intercondylar width between the first few postoperative days and 6 months postoperative was −0.92 mm (SD 1.9 mm, range: −5.5–1.7 mm). Two patients who underwent closed treatment of bilateral condylar fractures showed a relatively large amount of changes. Among them, one patient had the symphyseal fracture fixed with a miniplate, while the other patients had denture occlusion.</div></div><div><h3>Conclusions</h3><div>Long-term postoperative mandibular transverse width was comparatively stable. However, closed treatment of bilateral condylar fracture might be related to a decrease in the stability of mandibular transverse width.</div></div>","PeriodicalId":45034,"journal":{"name":"Journal of Oral and Maxillofacial Surgery Medicine and Pathology","volume":"37 4","pages":"Pages 649-653"},"PeriodicalIF":0.4,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144221604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preliminary evidence of helicobacter pylori in oral squamous cell carcinoma and oral premalignant disorders: A meta-analysis highlighting tissue associations","authors":"Gowri Sivaramakrishnan ,&nbsp;Kannan Sridharan","doi":"10.1016/j.ajoms.2024.12.024","DOIUrl":"10.1016/j.ajoms.2024.12.024","url":null,"abstract":"<div><h3>Introduction</h3><div>Oral squamous cell carcinoma (OSCC) is the most common head and neck cancer, linked to tobacco, alcohol, and HPV. The bacterium Helicobacter pylori (<em>H. pylori</em>), associated with gastrointestinal cancers, may also play a role in OSCC and oral premalignant disorders (OPMDs). This meta-analysis examines the potential association between <em>H. pylori</em>, OSCC, and its precursor lesions.</div></div><div><h3>Methodology</h3><div>Following PRISMA guidelines, databases were searched until July 30, 2024. Eligible studies focused on OSCC, OPMDs, and H. pylori. Data on study characteristics and detection methods were extracted. Bias was evaluated using the New Castle Ottawa Scale. Statistical analysis utilized a random-effects model in RevMan, reporting odds ratios (OR) and 95 % confidence intervals (CI). Heterogeneity was assessed with I².</div></div><div><h3>Results</h3><div>Twenty-three studies were included. Pooled estimates showed significantly more patients with OSCC and oral lichen planus (OLP) tested positive for H. pylori, with estimates of 0.17 [0.03, 0.31] and 0.19 [0.04, 0.34], respectively. Tissue sample studies indicated a significant association between H. pylori and OSCC (5.57 [3.23, 9.60]). However, data from other sample types were insufficient for pooled estimates.</div></div><div><h3>Conclusion</h3><div>This meta-analysis provides preliminary evidence of H. pylori's role in OSCC and OPMDs, suggesting its potential as a biomarker for early detection. Significant research gaps remain in <em>H. pylori</em> detection across various sample types, emphasizing the need for further studies, especially regarding non-invasive screening methods like saliva analysis.</div></div>","PeriodicalId":45034,"journal":{"name":"Journal of Oral and Maxillofacial Surgery Medicine and Pathology","volume":"37 4","pages":"Pages 599-606"},"PeriodicalIF":0.4,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144221565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MicroRNA-1260a suppression inhibits the growth of human oral squamous cell carcinoma cell lines","authors":"Hiroyuki Shirai,&nbsp;Koh-ichi Nakashiro ,&nbsp;Daisuke Uchida","doi":"10.1016/j.ajoms.2024.12.027","DOIUrl":"10.1016/j.ajoms.2024.12.027","url":null,"abstract":"<div><div>Oral squamous cell carcinoma (OSCC) is the most common malignant tumour among head and neck squamous cell carcinomas, with over 90 % cases histologically classified as squamous cell carcinoma. Despite advancements in chemotherapy, radiotherapy, and surgical techniques, the relative survival rate of patients with OSCC have shown little improvement over the past several decades. Two strategies can be considered to significantly improve treatment outcomes: early detection and novel therapeutic approach development for standard treatment-resistant OSCC. Here, we focused on microRNAs (miRNAs) as potential therapeutic targets in OSCC. Microarray and RT-PCR analyses revealed miRNA-1260a overexpression in primary OSCC tissues. Subsequently, the introduction of antisense oligonucleotides (ASOs) and synthetic tough decoys (S-TuDs) targeting miRNA-1260a significantly inhibited proliferation in human OSCC cell lines. Conversely, introducing the miRNA-1260a mimic into OSCC cells with high endogenous miRNA-1260a levels did not induce significant changes in proliferation. Finally, an RNA immunoprecipitation assay showed that miRNA-1260a targets tumour suppressor-like genes, such as ARHGAP24, CLMP, MOB1A, and PTPRK, in human OSCC cells. These results suggest that miRNA-1260a suppresses human OSCC proliferation, indicating its potential as a therapeutic target. Further investigations of miR-1260a and its regulatory mechanisms may provide valuable insights into novel treatment strategies for OSCC, ultimately aimed at improving patient outcomes.<span><span>²</span></span></div></div>","PeriodicalId":45034,"journal":{"name":"Journal of Oral and Maxillofacial Surgery Medicine and Pathology","volume":"37 4","pages":"Pages 639-648"},"PeriodicalIF":0.4,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144221605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiple organ tumor lesions analyzed using tumor-related immunostaining in a patient with codon 130 mutation of Cowden syndrome: A case report","authors":"Akiho Mori ,&nbsp;Shuhei Tsuchiya ,&nbsp;Keiji Matsumoto ,&nbsp;Kenichiro Ishibashi ,&nbsp;Kazuto Okabe ,&nbsp;Yasuyuki Shibuya","doi":"10.1016/j.ajoms.2024.12.020","DOIUrl":"10.1016/j.ajoms.2024.12.020","url":null,"abstract":"<div><div>Cowden syndrome (CS), also known as multiple hamartoma syndrome, is a rare autosomal dominant genodermatosis. We report a case of a 53-year-old woman diagnosed with CS through phosphatase and tensin homolog (PTEN) gene sequencing, which revealed a mutation in codon 130 of exon 5 (Arg130Ter). A mass appeared on the right buccal mucosa and tongue owing to misbiting. An excision biopsy was conducted, and the sample was analyzed via immunohistochemistry to understand why oral papillomatosis did not progress to malignancy. A review of PubMed identified 21 cases of CS with Arg130Ter mutation. No predilection for women was observed, with an equal distribution between men and women. The incidence rates of thyroid, breast, and urogenital neoplasms in patients with Arg130Ter mutation were higher than those in all patients with CS. Additionally, patients with the Arg130Ter mutation were diagnosed at a younger age than that in all patients with CS. Histopathological examination of the oral mucosa lesions revealed papillary fibroepithelial hyperplasia. Immunohistochemistry revealed negative results for PTEN, MIB-1, and S-100, but positive results for p53 and Bcl-2 in some epithelial cells. These results suggest that symptoms appear at a younger age and tend to be more severe, with p53 as a factor that suppresses malignant transformation in patients with Arg130Ter mutation in CS.</div></div>","PeriodicalId":45034,"journal":{"name":"Journal of Oral and Maxillofacial Surgery Medicine and Pathology","volume":"37 4","pages":"Pages 793-799"},"PeriodicalIF":0.4,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144221607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapidly growing primary squamous cell carcinoma of the submandibular gland: A rare case and literature review","authors":"Ya-qing Mao ,&nbsp;Jian-yun Zhang ,&nbsp;Rui Sun ,&nbsp;Chi Mao ,&nbsp;Xin Peng","doi":"10.1016/j.ajoms.2024.12.026","DOIUrl":"10.1016/j.ajoms.2024.12.026","url":null,"abstract":"<div><div>Primary squamous cell carcinoma (PSCC) is rare and aggressive in major salivary gland tumors, especially in the submandibular gland. In this report, we present a rare case of PSCC of the submandibular gland in an 81-year-old man, who is the oldest reported person with this disease. He was diagnosed with PSCC on the basis of clinical and imaging examinations and differential diagnosis with other salivary gland tumors through immunohistochemical staining and fluorescence in-situ hybridization. He underwent wide local excision of the submandibular gland with partial mandibulectomy and ipsilateral modified radical neck dissection. The patient subsequently received adjuvant radiotherapy and immunotherapy but died due to multiple pulmonary metastases 22 months after surgery. The features and findings of this case were compared with those of previously reported cases (n = 18) to shed light on the diagnosis, prognosis, and treatment of this disease. These findings indicate that this malignancy is very rare along with poor prognosis. Surgery is the basic treatment often supplemented by radiotherapy or chemotherapy, and further study is needed to figure out long-term outcomes.</div></div>","PeriodicalId":45034,"journal":{"name":"Journal of Oral and Maxillofacial Surgery Medicine and Pathology","volume":"37 4","pages":"Pages 829-834"},"PeriodicalIF":0.4,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144221614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synchronous mandibular squamous cell carcinoma and parotid gland salivary duct carcinoma ex-pleomorphic adenoma: A case report","authors":"Atsuro Noguchi ,&nbsp;Kenji Yamagata ,&nbsp;Natsumi Kawamatsu ,&nbsp;Shohei Takaoka ,&nbsp;Satoshi Fukuzawa ,&nbsp;Fumihiko Uchida ,&nbsp;Naomi Ishibashi-Kanno ,&nbsp;Hiroki Bukawa","doi":"10.1016/j.ajoms.2024.12.021","DOIUrl":"10.1016/j.ajoms.2024.12.021","url":null,"abstract":"<div><div>The upper gastrointestinal and respiratory tracts are the most common sites of synchronous cancers associated with oral squamous cell carcinoma (OSCC). To the best of our knowledge, synchronous OSCC and salivary duct carcinoma ex-pleomorphic adenoma (SDC ex-PA) have not been reported. A 51-year-old Japanese man was referred to our hospital with a complaint of dull pain in the mandible. He had been under observation for parotid mass that had not changed in size for 20 years. Contrast-enhanced magnetic resonance imaging revealed a 49 × 42 mm mandibular tumor and a 35 mm mass in the parotid gland with relatively well-defined borders. The left upper jugular and submandibular lymph nodes were enlarged. The clinical diagnosis was mandibular carcinoma (T4bN2bM0) and suspected pleomorphic adenoma. The patient was treated by tracheotomy, modified radical neck dissection, hemi-mandibulotomy, parotid tumor enucleation, and free fibula flap reconstruction under general anesthesia. The histopathology findings revealed that the mandibular tumor contained multilobed tumor cells that infiltrated and proliferated like foci. Tumor foci in the parotid gland were dispersed throughout the hyalinized stroma. The tumor cells had abundant and acidophilic cytoplasm, as well as nuclei of varying sizes and morphologies. Immunohistochemical staining revealed that the parotid tumor was positive for androgen receptor, human epidermal growth factor receptor type 2 and gross cystic disease fluid protein-15. The pathological diagnosis was well-differentiated SCC of the mandible (pT4bN0M0) and SDC ex-PA of the parotid gland (pT3N0M0). The surgical margin of parotid gland carcinoma was insufficient and required adjuvant chemoradiotherapy. The patient has remained disease-free for 2.5 years.</div></div>","PeriodicalId":45034,"journal":{"name":"Journal of Oral and Maxillofacial Surgery Medicine and Pathology","volume":"37 4","pages":"Pages 703-710"},"PeriodicalIF":0.4,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144222198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of prevalence and pattern of maxillary sinus septa based on three CBCT planes: New classification in the coronal plane","authors":"Ali Jamali ,&nbsp;Fereshteh Goudarzi ,&nbsp;Dani Stanbouly","doi":"10.1016/j.ajoms.2024.12.019","DOIUrl":"10.1016/j.ajoms.2024.12.019","url":null,"abstract":"<div><h3>Introduction</h3><div>Due to the presence of the maxillary sinus and its anatomic structures, such as septa, placing dental implants in the posterior maxilla can be challenging. This study aims to determine the occurrence, distribution, and shape of these septa in the maxillary sinus.</div></div><div><h3>Method</h3><div>Each patient's head was aligned and evaluated the presence of septa was in the remaining 381 CBCT images and 762 sinuses across the axial, coronal, and sagittal planes. The shape of the septa was assessed in the sagittal plane (based on Sigaroudi classification) and coronal planes. The variables were compared using chi-square and Pearson’s chi-square tests.</div></div><div><h3>Results</h3><div>In the study of 762 sinuses, we found 194 septa in 22.17 % of the sinuses. Most of the septa were located in the posterior third of the maxillary sinus. Based on the modified Al-Faraje classification, Types I and III were the most common septa found in the study. The shapes of the septa in the coronal plane were classified into 13 groups: short vertical septa, long vertical septa, horizontal line, curved line septa, oblique septa M, oblique septa MF, oblique septa LF, oblique septa ML, hazy shape, button-like, plate-like, semicircular and atypical. There was a significant correlation between the prevalence of septa and dental status, unlike age and sex.</div></div><div><h3>Conclusion</h3><div>Septa occurrence can have different classifications and radiological features. Clinicians should be aware of anatomical variations in Underwood septa and consider the shapes, patterns, and involved walls of the maxillary sinus to minimize potential complications.</div></div>","PeriodicalId":45034,"journal":{"name":"Journal of Oral and Maxillofacial Surgery Medicine and Pathology","volume":"37 4","pages":"Pages 624-630"},"PeriodicalIF":0.4,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144221568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of the molecular mechanism of claudin-1 expression in SCCKN cells, oral squamous cell carcinoma cell line","authors":"Tadayoshi Nobumoto ,&nbsp;Sachiko Yamasaki ,&nbsp;Atsuko Hamada ,&nbsp;Mirai Higaki ,&nbsp;Nanako Ito ,&nbsp;Fumitaka Obayashi ,&nbsp;Yasutaka Ishida ,&nbsp;Tomoaki Hamana ,&nbsp;Tomoaki Shintani ,&nbsp;Ryouji Tani ,&nbsp;Koichi Koizumi ,&nbsp;Souichi Yanamoto ,&nbsp;Yasutaka Hayashido","doi":"10.1016/j.ajoms.2024.12.017","DOIUrl":"10.1016/j.ajoms.2024.12.017","url":null,"abstract":"<div><h3>Objectives</h3><div>Expression of human double minute-2 (HDM2), a p53 ubiquitin ligase, increases the nuclear translocation of β-catenin and expression of claudin-1 (CLD1), a major component protein of tight junctions. Ligand of numb protein X1 (LNX1), an E3 ubiquitin ligase, binds to CLD1 and promotes its degradation by lysosomes in canine renal tubular epithelial cells. However, effect of LNX1 on CLD1 expression in oral squamous cell carcinoma (OSCC) remains unknown. Therefore, in this study, we aimed to elucidate the molecular mechanisms by which LNX1 affects CLD1 expression in OSCC cells.</div></div><div><h3>Methods</h3><div>We investigated the role of the β-catenin/TCF pathway in CLD1 expression to determine the relationship between LNX1 and CLD1 expression in SCCKN cell line, an OSCC cell line, via co-immunoprecipitation and mammalian two-hybrid assays. Additionally, we evaluated the effect of HDM2 on LNX1 expression in SCCKN cells.</div></div><div><h3>Results</h3><div>CLD1 expression was regulated via the β-catenin pathway, indicating that nuclear translocation of β-catenin upon HDM2 upregulation elevated the CLD1 levels in SCCKN cells. Inhibitor assays revealed that CLD1 protein in SCCKN cells was degraded by proteasomes or lysosomes. Co-immunoprecipitation and mammalian two-hybrid assays showed that CLD1 bound to LNX1 in SCCKN cells. Furthermore, LNX1p80 bound to HDM2 and underwent proteasomal or lysosomal degradation in SCCKN cells.</div></div><div><h3>Conclusions</h3><div>Our results suggest that HDM2-induced increase in LNX1p80 degradation suppresses CLD1 degradation by the proteasomes and lysosomes, thereby increasing the CLD1 levels in SCCKN cells.</div></div>","PeriodicalId":45034,"journal":{"name":"Journal of Oral and Maxillofacial Surgery Medicine and Pathology","volume":"37 4","pages":"Pages 607-616"},"PeriodicalIF":0.4,"publicationDate":"2024-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144221566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}