Transfusion Medicine (Oxford, England)最新文献

{"title":"Blood donation in Morocco: a 20-year retrospective study of blood collection in the Rabat blood centre.","authors":"A Tazi-Mokha,&nbsp;A Soulaymani,&nbsp;A Mokhtari,&nbsp;R Alami","doi":"10.1111/j.1365-3148.2012.01138.x","DOIUrl":"https://doi.org/10.1111/j.1365-3148.2012.01138.x","url":null,"abstract":"<p><strong>Objectives: </strong>We trace the history of blood donors and the recruitment strategies developed between 1988 and 2008 in the regional blood transfusion centre of Rabat. Beside, we draw a distribution map of the blood donors' population in Morocco during the year 2008.</p><p><strong>Background: </strong>Limited resource countries face considerable obstacles to ensuring a safe blood supply and safe blood transfusions. Many countries used and some of them still depend on replacement blood donors.</p><p><strong>Methods: </strong>Data published in this article had been collected from the CRTS of Rabat archives and reports. Data from all the 16 regional blood transfusion centres were collected from the report annually presented by the different regions.</p><p><strong>Results: </strong>We found that during the 20 years period studied, the number of blood units collected by Rabat recorded many fluctuations. In the mean time, many measures were taken to promote the recruitment of voluntary blood donors. The proportion of these later jumped from 16·55% in 1988 to reach almost 80% in 2008. Beside, the whole country's blood collection map for the year 2008 showed that 190 504 of whole blood units were collected. This means that there are only six donations per 1000 people.</p><p><strong>Conclusion: </strong>This is the first study conducted in Morocco that provided detailed information on ongoing trends in blood donor profiles. The creation and development of new mobile drive units and maintaining them over time was the key to increase the collection of blood units from voluntary, non-remunerated blood donors.</p>","PeriodicalId":442504,"journal":{"name":"Transfusion Medicine (Oxford, England)","volume":" ","pages":"173-80"},"PeriodicalIF":1.5,"publicationDate":"2012-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1365-3148.2012.01138.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40165916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular background of D-negative phenotype in the Tunisian population.","authors":"H Moussa,&nbsp;M Tsochandaridis,&nbsp;T Chakroun,&nbsp;S Jridi,&nbsp;B Abdelneji,&nbsp;S Hmida,&nbsp;M Silvy,&nbsp;P Bailly,&nbsp;J Gabert,&nbsp;A Levy-Mozziconacci,&nbsp;Saloua Jemni-Yacoub","doi":"10.1111/j.1365-3148.2012.01142.x","DOIUrl":"https://doi.org/10.1111/j.1365-3148.2012.01142.x","url":null,"abstract":"<p><strong>Background: </strong>Most studies of the molecular basis of Rhesus D-negative phenotype have been conducted in Caucasian and African populations. A comprehensive survey of RHD alleles was lacking in people from North Africa (Tunisians, Moroccans and Algerians) which could be very efficient for managing donors and patients carrying an RHD molecular variant. We analyse the molecular background of D-negative population in Tunisia in the present study.</p><p><strong>Materials and methods: </strong>Blood samples were collected from native Tunisians. A total of 448 D-negative donors from different regions of Tunisia were analysed by RHD genotyping according to an adopted strategy using real-time PCR, ASP-PCR and sequencing.</p><p><strong>Results: </strong>Among the 448 D-negative samples, 443 were phenotyped unequivocally as true D-negative including three molecular backgrounds which were RHD gene deletion (n = 437), RHDψ pseudogene (n = 2) and RHD-CE-D hybrid gene (n = 4) with the respective frequencies of 0·9900, 0·0023 and 0·0046. The remaining five samples, in discordance with the serological results, were identified as two weak D type 11, one weak D type 29, one weak D type 4·0 and one DBT-1 partial D.</p><p><strong>Conclusion: </strong>This study showed that the Tunisian population gets closer to Caucasians, given that the RHD gene deletion is the most prevalent cause of D-negative phenotype, but it is slightly different by the presence of the RHDψ pseudogene which was found with a very low frequency compared with that described in the African population. Nevertheless, the relative occurrence of weak D variants among studied serologically D-negative samples make necessary the adaptation of RHD genotyping strategy to the spectrum of prevalent alleles.</p>","PeriodicalId":442504,"journal":{"name":"Transfusion Medicine (Oxford, England)","volume":" ","pages":"192-8"},"PeriodicalIF":1.5,"publicationDate":"2012-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1365-3148.2012.01142.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40167963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nafamostat mesilate, a noncalcium compound, as an anticoagulant, induces calcium-dependent haemolysis when infused with packed erythrocytes.","authors":"H Fujita,&nbsp;R Sakuma,&nbsp;S Fujimoto,&nbsp;Y Hazama,&nbsp;C Ohtake,&nbsp;A Moriyama,&nbsp;K Kuhara,&nbsp;S Nishimura","doi":"10.1111/j.1365-3148.2012.01154.x","DOIUrl":"https://doi.org/10.1111/j.1365-3148.2012.01154.x","url":null,"abstract":"<p><strong>Background: </strong>Nafamostat mesilate (NM), a protease inhibitor, is available for acute pancreatitis and disseminated intravascular coagulopathy and is used as an anticoagulant for haemodialysis in Japan. Co-infusion of red cell concentrates (RCC) and intravenous drugs is usually contraindicated. Because of limited venous access, adherence to the guidelines may be compromised in some clinical settings. Therefore, we investigated the influence of co-infusion of RCC and various anticoagulants on haemolysis in vitro.</p><p><strong>Methods: </strong>We investigated the effect of co-incubation of RCC and various anticoagulant drugs [NM, gabexate mesilate (GM), heparin] in packed erythrocytes. We evaluated haemolysis using lactate dehydrogenase and free haemoglobin. In addition, we also evaluated the influence of co-incubation on phosphatidylserine (PS) expression on the erythrocyte membrane.</p><p><strong>Results: </strong>GM and NM induced haemolysis in a dose-dependent manner, which was inhibited by removal of citrate and pretreatment with the calcium chelator, ethylenediaminetetraacetic acid. In a dynamic experiment using an infusion pump, NM not only induced haemolysis during co-infusion with RCC but also elevated PS expression dependent on extracellular calcium.</p><p><strong>Conclusion: </strong>NM and GM induce haemolysis in packed erythrocytes in the presence of citrate that is dependent on extracellular calcium.</p>","PeriodicalId":442504,"journal":{"name":"Transfusion Medicine (Oxford, England)","volume":" ","pages":"186-91"},"PeriodicalIF":1.5,"publicationDate":"2012-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1365-3148.2012.01154.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40171143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Direct volumetric flow cytometric quantitation of CD34+ stem and progenitor cells.","authors":"K Gutensohn,&nbsp;A Nikolitsis,&nbsp;M Gramatzki,&nbsp;D Spitzer,&nbsp;U Buwitt-Beckmann,&nbsp;A Humpe","doi":"10.1111/j.1365-3148.2012.01155.x","DOIUrl":"https://doi.org/10.1111/j.1365-3148.2012.01155.x","url":null,"abstract":"<p><strong>Objectives: </strong>In this study, we compared a classic single-platform (SP) method applying beads for enumeration of CD45+ or CD34+ cells with a new device allowing direct volumetric measurements of stem and progenitor cells.</p><p><strong>Background: </strong>Following apheresis and cyropreservation, the precise enumeration of CD34+ cells as key parameter of graft quality is mandatory for the clinical course after transplantation. Currently, flow cytometry with SP technique represents the 'gold standard' for such determinations.</p><p><strong>Methods/materials: </strong>Fresh samples, 14 from mobilised peripheral blood (PB), 9 from apheresis products (AP) and 13 samples from frozen-thawed (FT) haematopoietic progenitor cell grafts, were analysed for CD34+ cells, CD45+ cells, and in frozen-thawed samples for viability by a bead-based flow cytometric method and in parallel by a direct, volumetric flow cytometric method.</p><p><strong>Results: </strong>Comparison of CD34+ analyses revealed a significant correlation (P < 0·01) for each material between both techniques with r = 0·95 (PB), r = 0·933 (AP) and r = 0·929 (FT). Also, for analysis of CD45+ cells µL(-1) , the measured numbers evaluated with the different techniques did not significantly differ for all three materials analysed. In frozen-thawed samples, the analysis of viability was comparable for both techniques.</p><p><strong>Conclusions: </strong>The results of this study demonstrate that a direct volumetric analysis of CD34+ cells µL(-1) or CD45+ cells µL(-1) is feasible. This technique represents a simple and economical approach for standardisation of progenitor and stem cell analyses.</p>","PeriodicalId":442504,"journal":{"name":"Transfusion Medicine (Oxford, England)","volume":" ","pages":"205-10"},"PeriodicalIF":1.5,"publicationDate":"2012-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1365-3148.2012.01155.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40170446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Consent to transfusion: patients' and healthcare professionals' attitudes towards the provision of blood transfusion information.","authors":"R Davis,&nbsp;C Vincent,&nbsp;A Sud,&nbsp;S Noel,&nbsp;R Moss,&nbsp;M Asgheddi,&nbsp;I Abdur-Rahman,&nbsp;M Murphy","doi":"10.1111/j.1365-3148.2012.01148.x","DOIUrl":"https://doi.org/10.1111/j.1365-3148.2012.01148.x","url":null,"abstract":"<p><strong>Background: </strong>Patients should be informed about the risks and benefits of blood transfusion and their consent should be documented. However, this is not routinely practised in the UK, and there have been few studies to investigate patients' and healthcare professionals' attitudes towards this process.</p><p><strong>Objectives: </strong>To investigate patients' and healthcare professionals' attitudes towards the information patients are provided with about transfusion and obtaining consent for transfusion.</p><p><strong>Measures: </strong>A cross-sectional qualitative survey design was employed. Attitudes towards transfusion-related information and consenting to transfusion were assessed using a patient survey and healthcare professional survey.</p><p><strong>Participants: </strong>One hundred and ten patients who had received a transfusion aged between 18 and 93 (60 males and 50 females) and 123 healthcare professionals (doctors, nurses and midwives) involved in administering transfusions.</p><p><strong>Results: </strong>Sixty-one patients recalled consenting transfusion. The majority said they were just told they needed a transfusion (N = 67) and only 1 patient said a full discussion about the risks and the benefits of the transfusion took place. However, although 82 patients said they were satisfied with the information, 22 patients reported they would have liked to have been given more details. The majority of healthcare professionals (N = 83) felt that patients were often not given sufficient information about transfusion.</p><p><strong>Conclusion: </strong>Greater efforts should be made to provide information to patients about the risks and benefits of blood transfusions. Future research should explore the most effective ways of delivering this information to patients in an appropriate and timely manner.</p>","PeriodicalId":442504,"journal":{"name":"Transfusion Medicine (Oxford, England)","volume":" ","pages":"167-72"},"PeriodicalIF":1.5,"publicationDate":"2012-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1365-3148.2012.01148.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40170979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Red blood cell storage and cell morphology.","authors":"B Blasi,&nbsp;A D'Alessandro,&nbsp;N Ramundo,&nbsp;L Zolla","doi":"10.1111/j.1365-3148.2012.01139.x","DOIUrl":"https://doi.org/10.1111/j.1365-3148.2012.01139.x","url":null,"abstract":"<p><strong>Aim: </strong>In this study, we performed weekly assessment of morphology-related parameters through monitoring of CPD-SAGM leuco-filtered erythrocyte concentrates from blood withdrawal until the 42nd day of storage.</p><p><strong>Background: </strong>Liquid storage of red blood cells (RBCs) delivers a blood-derived therapeutic, which is safe, available, effective and affordable for most patients who need transfusion therapy in developed countries. However, a growing body of accumulating controversial evidences, from either biochemical or retrospective clinical studies, prompted safety concerns about longer stored RBCs.</p><p><strong>Methods: </strong>Statistical image analysis through scanning electron microscope was coupled to osmotic fragility and erythrocyte sedimentation rate.</p><p><strong>Results: </strong>We could observe that by day 21 more than 50% of RBCs displayed non-discocyte phenotypes. This observation was related to an increase in osmotic fragility, which was totally overlapped in day 0 controls and day 7 RBCs while only slightly augmented in day 14 samples. Cation dysregulation (pH internal/external alteration and potassium) might both reflect and trigger a negative feedback loop with metabolic fluxes and membrane cation pumps.</p><p><strong>Conclusion: </strong>Morphology parameters suggest that significant alterations to RBC morphology over storage duration occur soon after the 14th day of storage, as to become significant enough within the 21st day.</p>","PeriodicalId":442504,"journal":{"name":"Transfusion Medicine (Oxford, England)","volume":" ","pages":"90-6"},"PeriodicalIF":1.5,"publicationDate":"2012-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1365-3148.2012.01139.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40141981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Haemolysis after treatment with intravenous immunoglobulin due to anti-A.","authors":"S Morgan,&nbsp;P Sorensen,&nbsp;G Vercellotti,&nbsp;N D Zantek","doi":"10.1111/j.1365-3148.2011.01078.x","DOIUrl":"https://doi.org/10.1111/j.1365-3148.2011.01078.x","url":null,"abstract":"<p><strong>Background: </strong>Intravenous immunoglobulin (IVIG) is used to treat an increasing number of conditions. IVIG contains immunoglobulin G (IgG) directed against many targets, including red blood cell (RBC) antigens.</p><p><strong>Methods/materials: </strong>We report on three patients identified within a 7-month period in a single institution who developed haemolysis because of passively transferred anti-A.</p><p><strong>Results: </strong>The patients were a 34-year-old A (non-A1) D-positive male with aplastic anaemia, a 61-year-old A1 D-negative female with myasthenia gravis and a 57-year-old AB D-positive female lung transplant recipient. The haemoglobin decreased from 11.1 to 5.3 g dL(-1) over 2 days, 12.8 to 7.8 g dL(-1) over 6 days and 7.8 to 6.0 g dL(-1) over several hours, respectively. All three patients had a negative antibody screen, positive direct antiglobulin test for IgG only and an elution containing anti-A1 reactivity. The patients were transfused with O RBC with an appropriate rise in haemoglobin.</p><p><strong>Conclusion: </strong>These cases illustrate the potential severity of haemolysis after IVIG because of passively transferred antibodies to blood group antigens. Lack of recognition of IVIG as a cause for haemolysis by clinicians may be further confounded if routine testing fails to detect the passively transferred ABO blood group antibodies.</p>","PeriodicalId":442504,"journal":{"name":"Transfusion Medicine (Oxford, England)","volume":" ","pages":"267-70"},"PeriodicalIF":1.5,"publicationDate":"2011-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1365-3148.2011.01078.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40103659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gene frequencies of platelet-specific antigens in Croatian population.","authors":"M Pavic,&nbsp;R Zadro,&nbsp;D Coen Herak,&nbsp;M Radic Antolic,&nbsp;S Dodig","doi":"10.1111/j.1365-3148.2009.00971.x","DOIUrl":"https://doi.org/10.1111/j.1365-3148.2009.00971.x","url":null,"abstract":"<p><p>The human platelet antigens (HPA) are genetically defined polymorphisms expressed on platelet membrane glycoproteins. As platelet antigens are very important in several clinical situations and in population genetics, we used the polymerase chain reaction with sequence-specific primers (PCR-SSP) to investigate HPA-1, -2, -3 and -5 allele frequencies in the Croatian population. The HPA frequencies obtained in 219 Croatians were: 1a-0.854, 1b-0.146, 2a-0.890, 2b-0.110, 3a-0.575, 3b-0.425, 5a-0.895 and 5b-0.105. These data are similar to the frequencies reported in most European studies with some significant differences in HPA-2 when compared with the Dutch and German population, in HPA-3 when compared with the Swiss population and in HPA-5 when compared with the Finnish population. The three most common condensed HPA genotypes in the Croatian population were: HPA-1a/a, -2a/a, -3a/b, -5-a/a (0.283), HPA-1a/a, -2a/a, -3a/a, -5-a/a (0.137) and HPA-1a/b, -2a/a, -3a/b, -5-a/a (0.087). Data obtained in this study can be used for better understanding and treatment of immune-mediated platelet disorders in our population.</p>","PeriodicalId":442504,"journal":{"name":"Transfusion Medicine (Oxford, England)","volume":" ","pages":"73-7"},"PeriodicalIF":1.5,"publicationDate":"2010-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1365-3148.2009.00971.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40034975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of blood donor deferral causes in the Trinidad and Tobago National Blood Transfusion Service.","authors":"K S Charles,&nbsp;P Hughes,&nbsp;R Gadd,&nbsp;C J Bodkyn,&nbsp;M Rodriguez","doi":"10.1111/j.1365-3148.2009.00968.x","DOIUrl":"https://doi.org/10.1111/j.1365-3148.2009.00968.x","url":null,"abstract":"<p><p>The majority of blood donations in Trinidad and Tobago are made as replacement by family members or friends. National Blood Transfusion Policy was drafted in 2007 to promote voluntary, repeated donation. The objective of this study is to assess the current rate and reasons for donor deferral, and the aim is to guide the proposed donor education and recruitment programme. A retrospective study of pre-donation deferral of prospective blood donors at the National Blood Transfusion Centre, Port of Spain, Trinidad and Tobago, was conducted. Records of all pre-donation deferrals over a 12-month period were studied. As many as 11,346 pre-donation screening interviews were conducted. There were 4043 (35.6%) deferrals. The most common reasons for donor deferral were exposure to high-risk sexual activity (27.6%), low haemoglobin 22.2% and hypertension 17.5%. Other reasons such as medication, chronic medical illness, tattoos, travel history, recent pregnancy, surgery or presentation outside the accepted age limit caused 33.8% of all deferrals and the majority (34.7%) of male deferrals. Low haemoglobin (44.5%) was the most common reason among females. The rate of deferral of voluntary donors was not significantly different from that for replacement donors (31.7 vs. 35.4%, P = 0.25). This study exposed a lack of public awareness as the principal reason for an unacceptably high rate of donor deferral. Donor education about selection criteria needs to be urgently addressed as an objective of the National Policy. Monitoring and evaluation of deferral rates and reasons could be used as one indicator of the effectiveness of the Policy.</p>","PeriodicalId":442504,"journal":{"name":"Transfusion Medicine (Oxford, England)","volume":" ","pages":"11-4"},"PeriodicalIF":1.5,"publicationDate":"2010-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1365-3148.2009.00968.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40043478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Solvent-detergent filtered (S/D-F) fresh frozen plasma and cryoprecipitate minipools prepared in a newly designed integral disposable processing bag system.","authors":"M El-Ekiaby,&nbsp;M A Sayed,&nbsp;C Caron,&nbsp;S Burnouf,&nbsp;N El-Sharkawy,&nbsp;H Goubran,&nbsp;M Radosevich,&nbsp;J Goudemand,&nbsp;D Blum,&nbsp;L de Melo,&nbsp;V Soulié,&nbsp;J Adam,&nbsp;T Burnouf","doi":"10.1111/j.1365-3148.2009.00963.x","DOIUrl":"https://doi.org/10.1111/j.1365-3148.2009.00963.x","url":null,"abstract":"summary. Solvent‐detergent (S/D) viral inactivation was recently adapted to the treatment of single plasma donations and cryoprecipitate minipools. We present here a new process and a new bag system where the S/D reagents are removed by filtration and the final products subjected to bacterial (0·2 μm) filtration. Recovered and apheresis plasma for transfusion (FFP) and cryoprecipitate minipools (400 ± 20 mL) were subjected to double‐stage S/D viral inactivation, followed by one oil extraction and a filtration on a S/D and phthalate [di(2‐ethylhexyl) phthalate (DEHP)] adsorption device and a 0·2 μm filter. The initial and the final products were compared for visual appearance, blood cell count and cell markers, proteins functional activity, von Willebrand factor (VWF) multimers and protein profile by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE). Tri (n‐butyl) phosphate (TnBP) was quantified by gas chromatography and Triton X‐45 and DEHP by high‐performance‐liquid chromatography (HPLC). General safety tests were by 6·5 mL/kg intravenous injection in rats. The treated plasmas and cryoprecipitates were very clear and the protein content and functionality, VWF multimers and SDS–PAGE profiles were well preserved. TnBP and Triton X‐45 were < 1 and <25 ppm, respectively, and DEHP (about 5 ppm) was less than it was in the starting materials. Blood cell counts and CD45, CD61 and glycophorin A markers were negative. There was no enhanced toxicity in rats. Thus, plasma and cryoprecipitate can be S/D‐treated in this new CE‐marked disposable integral processing system under conditions preserving protein function and integrity, removing blood cells, S/D agents and DEHP, and ensuring bacterial sterility. This process may offer one additional option to blood establishments for the production of virally inactivated plasma components.","PeriodicalId":442504,"journal":{"name":"Transfusion Medicine (Oxford, England)","volume":" ","pages":"48-61"},"PeriodicalIF":1.5,"publicationDate":"2010-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1365-3148.2009.00963.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40034976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}