Journal of medical and bioengineering最新文献_第4页

Quantification and Characterization of Allicin in Garlic Extract 大蒜提取物中大蒜素的含量测定与表征

Journal of medical and bioengineering Pub Date : 1900-01-01 DOI: 10.12720/jomb.5.1.24-27

Ho Jian Herng

{"title":"Quantification and Characterization of Allicin in Garlic Extract","authors":"Ho Jian Herng","doi":"10.12720/jomb.5.1.24-27","DOIUrl":"https://doi.org/10.12720/jomb.5.1.24-27","url":null,"abstract":"Garlic (Allium sativum) is a plant well known for its extensive use in traditional and modern medicine. Its healing properties are attributed to thiosulfinates, compounds formed through an enzymatic reaction when garlic cloves are crushed. One of the most prominent thiosulfinate found in garlic is diallyl thiosulfinate or allicin. \u0000The condition in this research mimics Malaysian climate conditions. This allows allicin to be studied and characterized for its uses in the local agricultural sector as potential urea inhibitor. A spectophotometric method is used in this research to quantify allicin found in garlic extract. L-cysteine is used to react with allicin in garic extract with the knowledge that one molecule of allicin reacts rapidly with two molecules of cysteine to form two molecules of S-allyl mercaptocysteine. 5,5’-dithiobis-(2-nitrobenzoic acid) (DTNB) is added to the mixture so that spectrophotometrically active 2-nitro-5-thiobenzoate (NTB) is formed after residual cysteine reacts with DTNB. Quantification of allicin is done at 30°C with garlic extract concentration ranging from 0.005 g/mL to 0.03 g/mL. This is followed by tests on allicin stability at pH 4 – 8 at 30°C and effects of temperature on allicin from 30°C to 85°C.","PeriodicalId":437476,"journal":{"name":"Journal of medical and bioengineering","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125111849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

The Effects of Novel AMPK Activator on Human Vascular Endothelial Cells 新型AMPK激活剂对人血管内皮细胞的作用

Journal of medical and bioengineering Pub Date : 1900-01-01 DOI: 10.12720/JOMB.3.2.144-148

Han‐Min Chen, Jiun‐Tsai Lin, Cheng-Yi Kuo, Chun-fang Huang

引用次数: 2

Influence of Feature Selection on Naïve Bayes Classifier for Recognizing Patterns in Cardiotocograms 特征选择对Naïve贝叶斯分类器心电图模式识别的影响

Journal of medical and bioengineering Pub Date : 1900-01-01 DOI: 10.12720/JOMB.2.1.66-70

M. Menai, Fatimah J. Mohder, Fayha Al-mutairi

引用次数: 28

Prediction of the Binding Affinities of PSD95 PDZ Domain in Complex with the CRIPT Peptide PSD95 PDZ结构域与CRIPT肽配合物结合亲和力的预测

Journal of medical and bioengineering Pub Date : 1900-01-01 DOI: 10.12720/JOMB.2.2.137-141

Junmei Wang

引用次数: 1

Indian perspective Architecture for Advancement of Biomedical Telemedicine and mHealth System 印度视角的生物医学远程医疗和移动医疗系统发展架构

Journal of medical and bioengineering Pub Date : 1900-01-01 DOI: 10.12720/JOMB.2.2.80-83

Changchuan Yin, Dongchul Yoo, S. Yau

引用次数: 0

Bioconversion of Ferulic Acid into High Value Metabolites by White Rot Fungi Isolated from Fruiting-Body of the Polypore Mushroom 多孔菌子实体白腐菌阿魏酸转化为高价值代谢物的研究

Journal of medical and bioengineering Pub Date : 1900-01-01 DOI: 10.12720/JOMB.2.3.168-172

K. Tanruean, N. Chandet, N. Rakariyatham

引用次数: 4

In Vitro Propagation of an Edible Bamboo Bam- busa Bambos and Assessment of Clonal Fidelity through Molecular Markers 食用竹竹的离体繁殖及分子标记克隆保真度评价

Journal of medical and bioengineering Pub Date : 1900-01-01 DOI: 10.12720/JOMB.2.4.257-261

M. Anand, J. Brar, A. Sood

{"title":"In Vitro Propagation of an Edible Bamboo Bam- busa Bambos and Assessment of Clonal Fidelity through Molecular Markers","authors":"M. Anand, J. Brar, A. Sood","doi":"10.12720/JOMB.2.4.257-261","DOIUrl":"https://doi.org/10.12720/JOMB.2.4.257-261","url":null,"abstract":" Abstract—An efficient and reproducible protocol has been established through the technique of forced axillary branching for the propagation of an important edible bamboo species namely Bambusa bambos. High frequency multiple shoot induction was achieved from nodal segments collected from elite genotype on Murashige and Skoog’s (MS) medium supplemented with 4.4 µM Benzylaminopurine (BAP) and 1.16 µM Kinetin (Kn). The size of explant and season greatly influenced the frequency of bud break. Rooting posed a major problem to be worked out in this particular species. Best rooting response was observed on 9.80 µM of Indole- 3 Butyric acid (IBA) with 60 ± 14.1 % rooting. In vitro raised plants were successfully acclimatized and established in the field conditions where they exhibited normal growth. In a bid to ascertain genetic fidelity, DNA was extracted by CTAB method and samples were analysed in 1.8% agarose gel electrophoresis. In the present study no variation was reported among the in vitro raised progeny and the mother plant in the banding profiles generated by the total of fifteen Random Amplified polymorphic DNA (RAPD) and Inter Simple Sequence Repeats (ISSR) markers. Hence, molecular analysis confirmed that these plants were genetically similar and can be used as elite plants.","PeriodicalId":437476,"journal":{"name":"Journal of medical and bioengineering","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122667470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 32

Proliferative Capacity of in Vitro Corneal Epithelium: Role of Acacia Honey in the Initial Step of Wound Healing 离体角膜上皮的增殖能力:金合欢蜂蜜在创面愈合初期的作用

Journal of medical and bioengineering Pub Date : 1900-01-01 DOI: 10.12720/JOMB.3.2.107-112

Choy Ker-Woon, N. A. Ghafar, C. K. Hui, Y. A. M. Yusof, Ng Sook Luan

引用次数: 3

Landmark-Based Geometric Morphometrics in Describing Facial Shape of the Sama-Banguingui Tribe from the Philippines 基于地标的几何形态计量学描述菲律宾sama - bananguigui部落的面部形状

Journal of medical and bioengineering Pub Date : 1900-01-01 DOI: 10.12720/JOMB.2.2.131-136

Olive S. Anies, M. Torres, M. M. E. Manting, C. Demayo

{"title":"Landmark-Based Geometric Morphometrics in Describing Facial Shape of the Sama-Banguingui Tribe from the Philippines","authors":"Olive S. Anies, M. Torres, M. M. E. Manting, C. Demayo","doi":"10.12720/JOMB.2.2.131-136","DOIUrl":"https://doi.org/10.12720/JOMB.2.2.131-136","url":null,"abstract":"Studies of human forms using traditional methods only show minimal variations. However, advances in image analysis and statistics have resulted to highly quantitative descriptions of detailed variations. To be able to describe detailed variations and to understand the human face of tribes practicing consanguineous marriages like the Sama-Banguingui tribe, landmark-based geometric morphometrics was used. Selected purebreed individuals (25 males and 21 females) of the tribe participated in the study. Face images were landmarked using the 28 anatomical landmarks defined in this study. Relative warps scores generated were used for the analysis of shape variations. Visualization of variations was done using histograms and boxplots. Results showed minor differences in the shapes of the whole face in RW2 and RW3 but not in RW1, RW4, RW5 and RW6 where variations observed were not significantly different. The similarity in the facial characters within and between sexes observed in this study could be attributed to common ancestry and possibly due to consanguinity. This study have shown that the tools of geometric morphometrics can be used for detailed quantification of variations in shapes of morphological structures. ","PeriodicalId":437476,"journal":{"name":"Journal of medical and bioengineering","volume":"282 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122915278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

In vitro Screening of Honey against Enterococcus spp. Biofilm 蜂蜜抗肠球菌生物膜的体外筛选

Journal of medical and bioengineering Pub Date : 1900-01-01 DOI: 10.12720/JOMB.3.1.23-28

Wen-Jie Ng, Kit-Yin Lim, Ju-Yee Chong, Karly Low

{"title":"In vitro Screening of Honey against Enterococcus spp. Biofilm","authors":"Wen-Jie Ng, Kit-Yin Lim, Ju-Yee Chong, Karly Low","doi":"10.12720/JOMB.3.1.23-28","DOIUrl":"https://doi.org/10.12720/JOMB.3.1.23-28","url":null,"abstract":"Honey is known widely as a remedial agent for its wound healing, antibacterial, antioxidant and anti- inflammatory properties. Enterococci, on the contrary are associated with biofilm formation on medical devices which lead to devastating infections. This study was conducted to investigate the inhibitory effect of honey on established biofilm and prevention of biofilm formation. The biofilms of Enterococcus spp. (ATCC 19433, ATCC 29212, LMG 16192 and LMG 16216) were cultivated in microtitre plates with the treatment of different types of honey (Malaysian Gelam honey and Manuka honey (UMF 10 and 15)). The estimation of biofilm biomass extension was determined by measuring absorbance at 570 nm wavelength. It was found that Manuka honey UMF 15 was the most effective in reducing established biofilm biomass as compared to Malaysian Gelam honey. Nevertheless, Malaysian Gelam honey was found to be effective in preventing biofilm formation of Enterococcus spp. as compared to Manuka honey. In brief, Malaysian Gelam honey is effective to prevent enterococcal biofilm fomation whereas Manuka honey can be recommended as a potential therapeutic agent for biofilm related enterococcal infections. ","PeriodicalId":437476,"journal":{"name":"Journal of medical and bioengineering","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127727431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9