{"title":"BIOCHEMICAL CHARACTERIZATION AND PARTIAL PURIFICATION OF A BACTERIOCIN LIKE-INHIBITORY SUBSTANCE PRODUCED FROM Bacillus sp. T68 STRAIN","authors":"Sultan KAYA ŞEN, H. Katı","doi":"10.18038/estubtda.882229","DOIUrl":"https://doi.org/10.18038/estubtda.882229","url":null,"abstract":"Bacteriocins are peptides produced by various types of bacteria. Members of the Bacillus genus are known to produce compounds with inhibitory activity in protein structure against pathogenic and non-pathogenic microorganisms. In this study, a bacteriocin-like inhibitory substance (BLIS) synthesized by Bacillus sp. T68 strain, which was previously isolated from soil, was characterized. T68 strain was grown on different media to produce bacteriocin. Crude BLIS obtained was tested by well diffusion method against indicator bacterium. It was investigated within the temperature range of 10-121 C and pH range from 3.0 to 10.0. It was examined in terms of different organic solvents, enzymes and chemicals. Extracellularly produced BLIS was partially purified by ammonium sulphate precipitation method and analyzed on SDS-PAGE. Activity of partially purified BLIS was investigated. It was observed that BLIS produced in Luria Bertani Broth medium was higher as compared to the other media against indicator bacterium at 30 °C for 24 h. BLIS activity maintained at low temperatures (10-40 °C) and lost completely at high temperatures (> 60 °C). It was detected that BLIS exhibited activity in the pH range between 5.0 and 10.0. The effect of EDTA on BLIS activity was slightly positive. Proteinase K and trypsin inhibited BLIS activity. Among the detergents, sodium dodecyl sulphate and Triton X-100 reduced BLIS activity, while Tween 20 and Tween 80 retained it. Additionally, it was determined that application of Tween 20 at 30 °C for 5 hours increased the BLIS activity by 40%. It was found that the solvents used other than butan 1-ol preserved the BLIS activity over 80%. Chloroform and isopropanol increased the BLIS activity slightly. An inhibitory zone formed by the BLIS corresponding to a molecular weight of about 15 kDa was detected. This BLIS exhibited stability over wide pH and temperature ranges and in organic solvent treatments.","PeriodicalId":436776,"journal":{"name":"Eskişehir Technical University Journal of Science and Technology A - Applied Sciences and Engineering","volume":"14 2","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"120812771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"PHOTOLUMINESCENCE PROPERTIES OF Nd3+, Yb3+ CODOPED Ga2O3 NANOPARTICLES","authors":"A. Dulda","doi":"10.18038/estubtda.912081","DOIUrl":"https://doi.org/10.18038/estubtda.912081","url":null,"abstract":"The availability of sensitive photon detectors and inexpensive lasers allowed us to explore more efficient fluorescent probes that will work in the second near-infrared optical window. In this study, the optical properties of Nd3+ and Yb3+ co-doped Ga2O3 nanoparticles were studied. In order to indicate the correlation between particle size, crystallinity, and optical property of present samples XRD, TEM, and Photoluminescence analyses were performed. Various excitation wavelengths and dopant concentrations were used to understand the energy transfer mechanism in Nd3+ and Yb3+ co-doped Ga2O3 nanoparticles. As the excitation wavelength increased from 325 nm to 477 and 515 nm, Yb3+ emission peak intensity decreased while Nd3+ emission peak intensity increased. This inverse relationship between the emission intensities of Yb3+ and Nd3+ ion showed the presence of energy transfer between them. The resulting emission peaks were broad and weak, indicating the presence of a non-radiative decay channel due to the crystal defects.","PeriodicalId":436776,"journal":{"name":"Eskişehir Technical University Journal of Science and Technology A - Applied Sciences and Engineering","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114820462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"ASYMPTOTIC ANALYSIS OF ACOUSTIC GREEN’S FUNCTIONS","authors":"Hazel Yücel, Yaǧmur Ece Uçar","doi":"10.18038/estubtda.1248808","DOIUrl":"https://doi.org/10.18038/estubtda.1248808","url":null,"abstract":"An asymptotic expansion of layered Green’s function is examination in a spatial domain and parametric approach is proposed by determining a small parameter to derive the shortened form of Green’s function for 3- and 4-layered structures. For qualitative testing and comparison of the exact Green’s function and asymptotic shortened Green’s functions associated with the measurement and source point in the same layer, we performed numerical calculations.","PeriodicalId":436776,"journal":{"name":"Eskişehir Technical University Journal of Science and Technology A - Applied Sciences and Engineering","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124988123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"ON THE EFFECTIVENESS OF PARAGRAPH VECTOR MODELS IN DOCUMENT SIMILARITY ESTIMATION FOR TURKISH NEWS CATEGORIZATION","authors":"Ali Yürekli","doi":"10.18038/estubtda.1175001","DOIUrl":"https://doi.org/10.18038/estubtda.1175001","url":null,"abstract":"News categorization, which is a common application area of text classification, is the task of automatic annotation of news articles with predefined categories. In parallel with the rise of deep learning techniques in the field of machine learning, neural embedding models have been widely utilized to capture hidden relationships and similarities among textual representations of news articles. In this study, we approach the Turkish news categorization problem as an ad-hoc retrieval task and investigate the effectiveness of paragraph vector models to compute and utilize document-wise similarities of Turkish news articles. We propose an ensemble categorization approach that consists of three main stages, namely, document processing, paragraph vector learning, and document similarity estimation. Extensive experiments conducted on the TTC-3600 dataset reveal that the proposed system can reach up to 93.5% classification accuracy, which is a remarkable performance when compared to the baseline and state-of-the-art methods. Moreover, it is also shown that the Distributed Bag of Words version of Paragraph Vectors performs better than the Distributed Memory Model of Paragraph Vectors in terms of both accuracy and computational performance.","PeriodicalId":436776,"journal":{"name":"Eskişehir Technical University Journal of Science and Technology A - Applied Sciences and Engineering","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114792859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"OPTIMIZATION OF CHROMIUM AND LEAD BIOSORPTION IN WASTEWATER USING 33 FACTORIAL DESIGN","authors":"B. Yazici, S. Malkoc, Ece Özgören, Nur Dursun","doi":"10.18038/estubtda.1068351","DOIUrl":"https://doi.org/10.18038/estubtda.1068351","url":null,"abstract":"In this study removing heavy metals, Cr (III), and Pb (II) from wastewater, Microorganism Trichoderma sp. biosorption was performed using Cr (III), and Pb (II) removal was taken into account. For this study, 3^3 Factorial Experiment Designs were used, and temperature (°C), biosorbent dosage (g/L), and pH were selected as the main factors for Cr (III), and Pb (II) metals and three levels of these factors were determined as low, medium, and high. In this study, which was carried out to increase the metal removal efficiency and biosorption capacity, the main factors and the significance of each interaction of these factors were examined with 3^3 Factorial Design. For this purpose, by conducting Analysis of Variance (ANOVA) via Response Surface Methodology and optimization, more detailed results were obtained regarding the factors affecting the efficiency of metal removal from wastewater.","PeriodicalId":436776,"journal":{"name":"Eskişehir Technical University Journal of Science and Technology A - Applied Sciences and Engineering","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122340125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"CLASSIFICATION OF SATELLITE IMAGES WITH DEEP CONVOLUTIONAL NEURAL NETWORKS AND ITS EFFECT ON ARCHITECTURE","authors":"Fadime Diker, Ilker Erkan","doi":"10.18038/estubtda.1165890","DOIUrl":"https://doi.org/10.18038/estubtda.1165890","url":null,"abstract":"Unlike traditional machine learning methods, deep learning methods that can learn from image, video, audio, and text data, especially recently with the increase in hardware power, are also increasing in success. Considering the success and benefits of deep learning methods in many different fields with increasing data, similar effects are expected in architecture. In this study, we focused on textures by going down to specifics rather than general images. In this direction, a total of 4500 satellite images belonging to cloud, desert, green areas and water bodies were classified in the model developed using deep convolutional neural networks. In the developed model, 0.97 accuracy for cloud images, 0.98 accuracy for desert images, 0.96 accuracy for green areas images and 0.98 accuracy for water bodies images were obtained in the classification of previously unused test data (675 images). Although there are similarities in the images of cloud and desert, and images of green areas and water bodies, this success in textures shows that it can be successful in detecting, analyzing, and classifying architectural materials. Successful recognition, analysis and classification of architectural materials and elements with deep convolutional neural networks will be able to facilitate the acquisition of appropriate and useful data through shape recognition among many data, especially at the information collection phase in the architectural design process. Thus, it will help to take more accurate decisions by obtaining more comprehensive data that cannot be obtained from manual data analysis. Learning the distinctive features for classification of data in deep convolutional neural networks also explains architectural design differences and similarities. This situation reveals the hidden relationship in the designs and thus can offer architects the opportunity to make creative and original designs.","PeriodicalId":436776,"journal":{"name":"Eskişehir Technical University Journal of Science and Technology A - Applied Sciences and Engineering","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116790138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"IMPROVING EXISTING HOUSING STOCKS A MODEL WITH ARTIFICIAL SMART SUPPORT","authors":"Murat Batuhan Zeybek, L. Y. Tokman","doi":"10.18038/estubtda.1166064","DOIUrl":"https://doi.org/10.18038/estubtda.1166064","url":null,"abstract":"Habitable housing and a healthy environment are a fundamental human right. In ensuring that existing housing stocks are re-acquired with artificial intelligence support, in making them healthy and sustainable, the development of a support intelligent system is the main topic of this study. The improvement of what is present provides a holistic benefit that has a return in economic, social, cultural and environmental contexts. It is increasingly important to take advantage of technology to make predictions and assessments with effective policies as well as effective strategies, algorithms, software development, and simulation in improvement projects. The goal is to preserve the resources and energy we have, reduce waste production to zero, reduce carbon footprint, and use the smart model supported by artificial intelligence in this study. Demolition and rebuilding should only be considered as the last resort, as the growth of environmental, cultural, waste areas, and waste resources and energy waste. Improving robust and functional buildings without demolishing them, adapting structures with cultural value to current technology, ensuring sustainability with a smart system can be achieved. In this study, it is aimed at achieving a ‘Smart Model’, which is supported by artificial intelligence, which is designed in light of examples around the world to evolve existing housing stocks with high environmental impact and resource utilization, into buildings with technology-appropriate equipment that have sustainable properties for resource use.","PeriodicalId":436776,"journal":{"name":"Eskişehir Technical University Journal of Science and Technology A - Applied Sciences and Engineering","volume":"85 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117265726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Özgüç Bertuğ Capunaman, Cemal Koray Bingöl, Benay Gürsoy
{"title":"FROM STITCHES TO DIGITS AND BACK: COMPUTATIONAL CROCHETING OF BRANCHING GEOMETRIES","authors":"Özgüç Bertuğ Capunaman, Cemal Koray Bingöl, Benay Gürsoy","doi":"10.18038/estubtda.1169938","DOIUrl":"https://doi.org/10.18038/estubtda.1169938","url":null,"abstract":"Crocheting is a hands-on craft that involves repetitive manipulation of a single continuous thread with a hook-like tool to generate surfaces and 3D forms. In a previous study, we have presented a parametric model [1] that generates crochet patterns of NURBS surfaces using a 10-stitches-by-10-rows swatch to account for all the physical variables that affect the crocheted object (i.e., yarn thickness, hook size, crafter’s grip). The dimensions of the previously crocheted tension swatches were used as the inputs of the crochet pattern generator algorithm, alongside the desired NURBS geometry, to generate individualized crochet patterns. These crochet patterns are text-based representations, similar to g-code in additive manufacturing, enabling the documentation and communication of the step-by-step hands-on crocheting process. Following these crochet patterns, the users can crochet physical objects with the same dimensions and form as their digitally modeled counterparts. \u0000This paper presents the second stage of this research in which we expanded this computational framework to enable crocheting of parametric branching geometries with multiple components by multiple crafters. While the components of the branching geometries can be crocheted by a single user, it is also possible to have different users crochet the components since the tension swatch can capture crafter-specific variables. As a proof-of-concept, a branching structure made of 14 unique components is designed and crocheted by two students of architecture as part of the Advanced Digital Fabrication course at the Pennsylvania State University. The students each crocheted 7 components based on their individual inputs while maintaining the dimensions and form of the digitally designed branching geometry. The findings suggest the possibility of a collective and distributed crocheting platform which can be used to create crocheted artifacts in various scales. This can be considered an alternative way to transition from the digital to the physical without relying on digital fabrication tools.","PeriodicalId":436776,"journal":{"name":"Eskişehir Technical University Journal of Science and Technology A - Applied Sciences and Engineering","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128722598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"DEMYSTIFYING MACHINE LEARNING FOR ARCHITECTURE STUDENTS","authors":"A. Sorguç, Müge Kruşa Yemişcioğlu, O. Yetkin","doi":"10.18038/estubtda.1169816","DOIUrl":"https://doi.org/10.18038/estubtda.1169816","url":null,"abstract":"With the developments in technology, mass data, new approaches/tools, and the increasing inclusion of machine learning applications, the necessity to teach these concepts and their applications have emerged in all research areas including architecture. In this context, a new course named “machine learning applications in architecture” containing lectures on data, data literacy, patterns, and various kinds of models along with a project conducted by the students was developed and started to be taught in spring’2020. Conducting a class on relatively new subjects for students was a great challenge. Yet, with a well-defined problem-based learning approach, the adaptation of students to the subject took place immediately. It is important to note that as students are equipped with information on machine learning concepts and applications with the given lectures, they were free to choose the project topics of their own which are believed to be one of the reasons for the success of the end results. \u0000As a result of this class, the project topics varied widely as coloring a given painting, predicting the era of a building, interpreting 2d drawings for 3d modeling, optimizing daylight gain, analyzing distinctive features of data in a city, and visualizing data to represent various aspects in data. The outcomes of the class are documented and analyzed to show how information in different fields such as computer science, engineering, statistics, and so on can broaden their thinking of how to attack problems in the architectural design domain. Finally, topics such as data, data literacy, pattern recognition, and intelligent models are projected to play a key role in the future of design education since it provides an interdisciplinary ground to think about problems at hand from a distinct perspective.","PeriodicalId":436776,"journal":{"name":"Eskişehir Technical University Journal of Science and Technology A - Applied Sciences and Engineering","volume":"32 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121789522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"IMPROVEMENT OF VOLTAGE STRESS ON MOTOR CONTROL HARDWARE VIA OPTIMAL LOCATION OF RC SNUBBER CIRCUIT","authors":"Mehmet Emin Sariaydin, Şener Ağalar","doi":"10.18038/estubtda.1062978","DOIUrl":"https://doi.org/10.18038/estubtda.1062978","url":null,"abstract":"While the increase in the customer failure rate in the consumer electronics sector affects the manufacturers negatively in terms of cost. It has also become one of the issues that cause customer loss and customer satisfaction. In addition to the economic losses, during the production and distribution of the spare parts and products that are changed at the customer's home due to the reliability problems experienced in the field, extra carbon emissions are released to the nature for this operation. For these reasons, it has become important to reduce the rate of customer failure. \u0000With the increase in electronic components, the quality and reliability precautions that may occur in the electronic circuits, have become the subject of study today. As in many white goods products, it is advantageous to have variable speed motors in refrigerators due to energy efficiency and energy regulation issues. For this reason, the compressor of refrigerator comes to the forefront as the component that dissipates the highest power proportionally. With the use of variable speed compressors, the need for an electronic board with including power conversion and motor driving capability has arisen. \u0000In this paper, RC Snubber circuits have been studied in order to prevent quality problems that may occur due to high switching frequency in flyback converter topology, which is one of the DC-DC converters that provide isolation. The methods in the literature based on the location of the RC Snubber in the circuit and the determination of its related values were examined and experiments were made on the simulation program. At the same time, a test mechanism was set up on the variable speed compressor motor control card of the refrigerator and measurements were taken with the help of an oscilloscope. The measurements taken in the simulation program and the experimental environment were compared.","PeriodicalId":436776,"journal":{"name":"Eskişehir Technical University Journal of Science and Technology A - Applied Sciences and Engineering","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115430908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}