Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy最新文献_第8页

Advancing anaerobic microbial studies with in situ Raman spectroscopy: Methanogenic archaea as a model

IF 4.3 2区化学

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2025-03-12 DOI: 10.1016/j.saa.2025.126043

Jintao Zhuo , Rikuan Zheng , Zhendong Luan , Lianfu Li , Shichuan Xi , Zengfeng Du , Wanying He , Chaomin Sun , Xin Zhang

{"title":"Advancing anaerobic microbial studies with in situ Raman spectroscopy: Methanogenic archaea as a model","authors":"Jintao Zhuo , Rikuan Zheng , Zhendong Luan , Lianfu Li , Shichuan Xi , Zengfeng Du , Wanying He , Chaomin Sun , Xin Zhang","doi":"10.1016/j.saa.2025.126043","DOIUrl":"10.1016/j.saa.2025.126043","url":null,"abstract":"<div><div>Methanogenic archaea play a critical role in methane (CH<sub>4</sub>) production and the global carbon cycle, yet accurately monitoring their gas metabolism under anaerobic conditions remains a technical challenge. In this study, we developed a Raman spectroscopy-based gas quantification model, achieving high-precision monitoring of CO<sub>2</sub>–N<sub>2</sub>–CH<sub>4</sub> ternary gas mixtures over a temperature range of 12–52 °C. The model exhibited strong linear correlations between the Raman peak area ratios and gas molar ratios, which were further validated against gas chromatography, revealing no significant differences (<em>p</em> > 0.05). This confirms the reliability and accuracy of the approach.. Building upon this model, we conducted real-time monitoring of the gas metabolism of methylotrophic methanogenic archaea under anaerobic conditions. The results demonstrated that methanol concentration significantly influenced the gas production kinetics. At a methanol concentration of 10 μL/mL, the highest CH<sub>4</sub> yield (59.97 %) was achieved, along with stable metabolic activity. In contrast, higher concentrations caused substrate saturation effects, leading to decreased metabolic efficiency. Furthermore, by integrating Raman spectroscopy with high-precision pressure monitoring, this study successfully achieved real-time molar quantification of CH<sub>4</sub> and CO<sub>2</sub> during methanogen cultivation. This approach provided detailed insights into gas production dynamics and substrate utilization patterns. Compared to traditional methods, this non-destructive, real-time monitoring platform offers a novel tool for anaerobic metabolism research and lays a solid foundation for applications in biogas optimization, industrial fermentation, and renewable energy development.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"336 ","pages":"Article 126043"},"PeriodicalIF":4.3,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143620729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Changes in absorption spectra of indocyanine green after visible light exposure and cold dark storage

IF 4.3 2区化学

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2025-03-12 DOI: 10.1016/j.saa.2025.126048

Magdalena Szpunar , David Aebisher , Andrzej Wal

{"title":"Changes in absorption spectra of indocyanine green after visible light exposure and cold dark storage","authors":"Magdalena Szpunar , David Aebisher , Andrzej Wal","doi":"10.1016/j.saa.2025.126048","DOIUrl":"10.1016/j.saa.2025.126048","url":null,"abstract":"<div><div>Indocyanine green (ICG) is commonly used as a fluorescent cardiovascular imaging agent, but its stability under different conditions remains insufficiently studied. This research examines the photochemical stability and aggregation behaviour of ICG in aqueous solutions subjected to visible light irradiation and during cold, dark storage. ICG solutions at different concentrations were exposed to visible light, and changes in absorption spectra were monitored over time. Exponential decreases in the absorption maxima were observed for both monomeric and dimeric ICG during irradiation. These changes were modelled, allowing for the description of decay processes caused by irradiation for monomers and dimmers, respectively. Furthermore, after a month of dark storage at 4 °C, a decrease in absorption was observed, accompanied by the formation of J-aggregates. These results indicate that ICG undergoes significant structural changes that may affect its fluorescence properties, with potential applications for use in photodynamic therapy. More research is needed to explore the impact of these changes on the performance of ICG in vivo.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"336 ","pages":"Article 126048"},"PeriodicalIF":4.3,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143643900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel dual-function peptide-based fluorescent probe with large Stokes shift for the simultaneous detection and quantification of Ag(I) and Hg(II) ions

IF 4.3 2区化学

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2025-03-12 DOI: 10.1016/j.saa.2025.126032

Chunmei Pu, Shiyang Li, Xinlin Cao, Yi Ren, Ruihan Bai, Fengling You, Peng Wang

{"title":"A novel dual-function peptide-based fluorescent probe with large Stokes shift for the simultaneous detection and quantification of Ag(I) and Hg(II) ions","authors":"Chunmei Pu, Shiyang Li, Xinlin Cao, Yi Ren, Ruihan Bai, Fengling You, Peng Wang","doi":"10.1016/j.saa.2025.126032","DOIUrl":"10.1016/j.saa.2025.126032","url":null,"abstract":"<div><div>Achieving highly selective detections of Ag<sup>+</sup> and Hg<sup>2+</sup> is of great importance because their excessive emission can cause many diseases. Herein, a novel large Stokes shift fluorescent probe <strong>DKT</strong> based on dansyl fluorophore modified peptide biomolecules (NH<sub>2</sub>-Thr-Lys-Thr-NH<sub>2</sub>) was successfully synthesized, which can simultaneously detect Ag<sup>+</sup> (turn on) and Hg<sup>2+</sup> (turn off) based on different fluorescent response patterns in 100% aqueous solution. <strong>DKT</strong> also enables quantitative detection of Ag<sup>+</sup> and Hg<sup>2+</sup> with LODs of 37.1 nM and 26.7 nM, respectively. Besides, ESI-HRMS spectra, Job’s plot, and fluorometric titration confirmed that the binding stoichiometry was determined to be 2: 1 between <strong>DKT</strong> and Ag<sup>+</sup>/Hg<sup>2+</sup>. Moreover, <strong>DKT</strong> has good fluorescence stability and rapidly detected Ag<sup>+</sup> and Hg<sup>2+</sup> over a wide pH range. Furthermore, <strong>DKT</strong> was applied to quantitative detect Ag<sup>+</sup> and Hg<sup>2+</sup> in three actual water samples, three tea samples and watermelon juice, which exhibited that <strong>DKT</strong> has good potential for accuracy in environmental monitoring and water quality control. Additionally, the results of fluorescence bioimaging showed that <strong>DKT</strong> had significant distinguishing ability to detect Ag<sup>+</sup> and Hg<sup>2+</sup> in living cells and zebrafish larvae. More importantly, smartphone <em>App</em> platform based on <strong>DKT</strong> not only provided a reliable new method for the field quantitative monitoring of Ag<sup>+</sup> and Hg<sup>2+</sup>, but also expanded its application prospect in the field of heavy metal ions pollution detection.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"336 ","pages":"Article 126032"},"PeriodicalIF":4.3,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143629428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ARCHER. A new algorithm for automatic removal of cosmic spikes and saturated pixels in hyperspectral Raman spectroscopy

IF 4.3 2区化学

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2025-03-12 DOI: 10.1016/j.saa.2025.126041

Enmanuel Cruz Muñoz , Davide Ballabio , José Manuel Amigo

引用次数: 0

Split T7 switch-based orthogonal logic operation of fluorogenic RNA aptamer for small molecules detection

IF 4.3 2区化学

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2025-03-12 DOI: 10.1016/j.saa.2025.126044

Hao Liu, Jia-Min Peng, Cheng-Jun Zha, Mei Su, Zhan-Ming Ying

{"title":"Split T7 switch-based orthogonal logic operation of fluorogenic RNA aptamer for small molecules detection","authors":"Hao Liu, Jia-Min Peng, Cheng-Jun Zha, Mei Su, Zhan-Ming Ying","doi":"10.1016/j.saa.2025.126044","DOIUrl":"10.1016/j.saa.2025.126044","url":null,"abstract":"<div><div>Recent advances in fluorescent biosensors have stimulated the development of molecular detection. We herein developed a new orthogonal logic operation of fluorescent biosensor with cell-free to accomplish the detection of atrazine (ATZ) and tetrachlorobiphenyls (PCB77). The transcriptional process to generate fluorescent RNA aptamers (Mango) was controlled by molecules-probe bindings, which regulate split T7 promoter transcription switches ON or OFF. Leveraging the rapid in vitro T7 transcription process and high signal-to-background ratio of the Mango-TO1-Biotin complex, this biosensor demonstrates remarkable sensitivity in detecting ATZ and PCB77, with detection limit of 1.56 pM and 10.2 pM. Moreover, the orthogonality of four logic gates (AND, NOR, INHIBT, NIMPLY) were utilized the ATZ and PCB77 as input to construct, which could be activated by utilizing the target probe-driven association. The output of the fluorescence signal was controlled by split/intact fluorescent RNA aptamer (Mango) to achieve flexible and sensitive orthogonal operations. Significantly, the development of two-input logic gates has enabled the modular detection of various small molecules, offering a promising approach to intelligent multi-input analysis. Predictably, with the advantages of sensitivity, flexibility and easy-to-operate, this orthogonal logic gate platform holds immense potential in small molecules detection.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"336 ","pages":"Article 126044"},"PeriodicalIF":4.3,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143620727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The encapsulation of pyrene-substituted amphiphilic polymers within the interfaces of hydrophobic/hydrophilic core-shell silica gel for the detection of nitroaromatic compounds

IF 4.3 2区化学

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2025-03-12 DOI: 10.1016/j.saa.2025.126047

Bengi Ozgun Ozturk, Aleyna Hillik

{"title":"The encapsulation of pyrene-substituted amphiphilic polymers within the interfaces of hydrophobic/hydrophilic core-shell silica gel for the detection of nitroaromatic compounds","authors":"Bengi Ozgun Ozturk, Aleyna Hillik","doi":"10.1016/j.saa.2025.126047","DOIUrl":"10.1016/j.saa.2025.126047","url":null,"abstract":"<div><div>This study reports the encapsulation of pyrene-substituted amphiphilic polymers within void interfaces of mesoporous core–shell silica gel, specifically engineered for fluorescence-based detection of NACs in both aqueous and organic media. The hydrophobic shell of the silica gel enhances the diffusion of hydrophobic NACs to the silica gel core, where the confined pyrene-substituted amphiphilic polymer acts as a sensor, enabling efficient fluorescence quenching in both aqueous and organic media. The sensor exhibited strong quenching responses, particularly for TNT, with a Stern-Volmer constant of 7.51 × 10<sup>4</sup> M<sup>−1</sup> in dichloromethane and 6.30 × 10<sup>4</sup> M<sup>−1</sup> in water. The sensor also demonstrated excellent reusability, retaining over 95 % of its original fluorescence intensity after four quenching cycles. These results highlight the potential of pyrene-encapsulated core–shell silica gels as highly sensitive, reusable fluorescence sensors for NAC detection, offering promising environmental monitoring and security applications.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"336 ","pages":"Article 126047"},"PeriodicalIF":4.3,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143636738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to “A polarity-sensitive fluorescent probe for visualizing lipid droplets in ferroptosis, cuproptosis, and autophagy” [Spectrochim. Acta Part A: Mol. Biomol. Spectrosc. 332 (2025) 125854]

IF 4.3 2区化学

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2025-03-12 DOI: 10.1016/j.saa.2025.125959

Yang Lv , Haoyu Jin , Zhe Liu , Na Li , Ye-Xin Liao , Jianliang Shen , Ji-Ting Hou

引用次数: 0

A Golgi apparatus-targeted ratiometric fluorescent probe for HOCl and its applications for anti-inflammatory evaluation of Dachengqi Decoction

IF 4.3 2区化学

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2025-03-11 DOI: 10.1016/j.saa.2025.126026

Li Yang , Xuan Zhao , Yan Liu , Ye-Xin Liao , Yuyu Fang , Ji-Ting Hou , Shan Wang

{"title":"A Golgi apparatus-targeted ratiometric fluorescent probe for HOCl and its applications for anti-inflammatory evaluation of Dachengqi Decoction","authors":"Li Yang , Xuan Zhao , Yan Liu , Ye-Xin Liao , Yuyu Fang , Ji-Ting Hou , Shan Wang","doi":"10.1016/j.saa.2025.126026","DOIUrl":"10.1016/j.saa.2025.126026","url":null,"abstract":"<div><div>Verifying the fluctuations of reactive oxidative species in the Golgi apparatus (GA) is crucial to investigate the pathology in inflammation. In this work, we present a fluorescent probe <strong>GA-PBC</strong> based on the phenothiazine-coumarin chromophore with a benzenesulfonamide unit as the GA target. <strong>GA-PBC</strong> manifests a red fluorescence peak at 620 nm, while it shifts to 510 nm upon reaction with HOCl, thus facilitating a ratiometric sensing manner. The probe shows high selectivity, superb sensitivity (limit of detection: 85.8 nM), rapid response (within seconds), and predominant accumulation in the GA. Intracellular imaging tests demonstrates the ability of <strong>GA-PBC</strong> to indicate the concentration changes of HOCl in live cells. Especially, it can be utilized to identify the active ingredients of Dachengqi Decoction (a kind of Traditional Chinese Medicine formula) in inflamed cells using HOCl in the GA as biomarker, suggesting that this probe is potentially useful for the evaluation of anti-oxidation efficacy of natural medicine.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"336 ","pages":"Article 126026"},"PeriodicalIF":4.3,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143642340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A near-infrared “turn-on” fluorescent probe for selective detection of copper(II) ions in aqueous media and its application in cell imaging

IF 4.3 2区化学

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2025-03-11 DOI: 10.1016/j.saa.2025.126036

Hang Zhou, Sheng Wang, Yu Jin, Xiu-fen Pang, Qing Zhao, Ting Zhang, Jian Zhang

引用次数: 0

Detection of starch content in maize kernel based on Raman hyperspectral imaging technique

IF 4.3 2区化学

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2025-03-11 DOI: 10.1016/j.saa.2025.126025

Yuan Long , Qingyan Wang , Xiuying Tang , Wenqian Huang , Bin Zhang

{"title":"Detection of starch content in maize kernel based on Raman hyperspectral imaging technique","authors":"Yuan Long , Qingyan Wang , Xiuying Tang , Wenqian Huang , Bin Zhang","doi":"10.1016/j.saa.2025.126025","DOIUrl":"10.1016/j.saa.2025.126025","url":null,"abstract":"<div><div>Starch is an important component for maize to ensure the quality of maize kernels. However, maize aging has a negative effect on the quality. In this study, Raman hyperspectral imaging technique was employed to detect the starch content during maize aging. The typical Raman peaks were analyzed and two-dimensional correlation spectroscopy (2D-COS) was applied to further characterize the relationship between Raman peaks and maize aging process. The changes order of Raman peaks under the effect of maize aging were revealed. Six preprocessing methods and five modeling methods were used to establish the maize starch content prediction model. The characteristic Raman shifts extracted by variable selection methods and 2D-COS peaks were used as inputs to establish the prediction model, and further fused to explore the model. The results showed that ELM model coupled with 35 characteristic Raman shifts based on normalization preprocessing method exhibited the optimal performance with Rc, Rp, RMSEC and RMSEP of 0.8827, 0.8502, 0.0539, 0.0577, respectively. In conclusion, this study provided a feasibly and powerfully nondestructive detection method for the determination of maize starch during maize aging and laid a theoretical foundation in the cereal detection industry.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"336 ","pages":"Article 126025"},"PeriodicalIF":4.3,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143642341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0