用拉曼光谱监测活细胞对慢病毒感染的实时反应研究

IF 4.6 2区化学 Q1 SPECTROSCOPY

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2025-06-03 DOI:10.1016/j.saa.2025.126535

Rheta Elkhaira, Keita Iwasaki, Hidetoshi Sato

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引用次数: 0

摘要

本研究的目的是研究活细胞对病毒入侵的实时反应。我们分别用重组慢病毒（rLV）和HEK293细胞作为模型病毒和模型细胞。rLV不能在细胞内繁殖，但将其基因，绿色荧光蛋白（GFP）整合到宿主细胞的基因组中。利用拉曼光谱和主成分分析（PCA）监测了病毒侵袭过程中细胞分子组成的变化。受病毒感染的细胞仅在感染后2h就出现了分子变化，主要是蛋白质引起的。这表明，这些分子变化代表了细胞对病毒入侵的早期反应，因为这些变化发生得太早，无法激活病毒诱导的反应。我们成功地检测了活细胞在遇到第二次病毒感染时的分子变化。这些结果有力地表明，细胞有一种机制来检测病毒入侵其体内，并启动对入侵的快速反应。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Study on real time response of live cells to lentivirus infection monitored by Raman spectroscopy

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Study on real time response of live cells to lentivirus infection monitored by Raman spectroscopy

The purpose of the present study is to investigate real time responses of live cells to　virus invasion. We employ recombinant lentivirus (rLV) and HEK293 cell for a model virus and a model cell, respectively. The rLV is not able to propagate in the cell but integrates its gene, green fluorescence protein (GFP) in the present case, into the genome of the host cell. Molecular compositional changes in the cells have been monitored during the viral invasion activity using Raman spectroscopy and principal components analysis (PCA). The cell with virus infection showed molecular changes which mostly attributed to protein at only 2 h post infection. It suggests that the molecular changes represent a very early cellular reaction against the viral invasion, because the changes have taken place too early to activate the reactions induced by the virus. We have successfully detected the molecular changes of the live cells also when they met the second viral infection. The results strongly suggest that the cell has a mechanism to detect the viral invasion into its body and initiates a quick reaction against the invasion.

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来源期刊

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 物理-光谱学

CiteScore

8.40

自引率

11.40%

发文量

1364

审稿时长

40 days

期刊介绍： Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science. The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments. Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate. Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to: Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences, Novel experimental techniques or instrumentation for molecular spectroscopy, Novel theoretical and computational methods, Novel applications in photochemistry and photobiology, Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.