Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy最新文献

{"title":"CH3/CF3 substituent dependent tunable fluorescence and thermofluorochromism triphenylamine-acetophenone fluorophore","authors":"Parthasarathy Gayathri ,&nbsp;Sasikala Ravi ,&nbsp;R. Rameshbabu Priyadharsan ,&nbsp;Mehboobali Pannipara ,&nbsp;Abdullah G. Al-Sehemi ,&nbsp;Vedichi Madhu ,&nbsp;Dohyun Moon ,&nbsp;Savarimuthu Philip Anthony","doi":"10.1016/j.saa.2025.126962","DOIUrl":"10.1016/j.saa.2025.126962","url":null,"abstract":"<div><div>Triphenylamine-acetophenone donor-acceptor fluorophore with methyl ((E)-3-(4-(diphenylamino)phenyl)-1-(p-tolyl)prop-2-en-1-one (<strong>1-CH</strong>_{<strong>3</strong>})) and trifluoromethyl ((E)-3-(4-(diphenylamino)phenyl)-1-(4-(trifluoromethyl)phenyl)prop-2-en-1-one (<strong>1-CF</strong>_{<strong>3</strong>})) substituents were synthesized and explored the substituent effect on the photophysical properties in solution as well as solid-state. <strong>1-CH</strong>_{<strong>3</strong>} and <strong>1-CF</strong>_{<strong>3</strong>} showed solvent polarity dependent tunable fluorescence between 489 and 564 nm (quantum yield (ϕ_F) = 0.258 (toluene) compared to quinine sulfate). <strong>1-CH</strong>_{<strong>3</strong>} and <strong>1-CF</strong>_{<strong>3</strong>} also exhibited strong green and yellow fluorescence in the solid-state at 535 and 570 nm, respectively. The change of substituent significantly altered the melting point, <strong>1-CH</strong>_{<strong>3</strong>} melted at 110 °C whereas <strong>1-CF</strong>_{<strong>3</strong>} melts at 55 °C. The low melting point of <strong>1-CF</strong>_{<strong>3</strong>} enabled to achieve thermoresponsive off-on reversible fluorescence switching in solids, aggregated state and polymer matrix at near room temperature. Both <strong>1-CH</strong>_{<strong>3</strong>} and <strong>1-CF</strong>_{<strong>3</strong>} exhibited strong reduction of fluorescence intensity upon crushing. But only <strong>1-CF</strong>_{<strong>3</strong>} showed recovering of fluorescence with time or by solvent exposure. Data encryption application was demonstrated using the thermoresponsive <strong>1-CF</strong>_{<strong>3</strong>} by integrating with polymer matrix (PMMA) and fabricating thin films. <strong>1-CF</strong>_{<strong>3</strong>}-polmer exhibited complete disappearance of fluorescence upon heating and reversed to initial upon cooling at −15 °C for a minute. Thus, the present work elucidated the role of substituent group for developing functional fluorescent molecules for reversible temperature dependent fluorescence switching applications.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"346 ","pages":"Article 126962"},"PeriodicalIF":4.6,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145118283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Upconversion luminescence reversible modulation of photochromic NaYF4: Yb, Er @N-TiO2 core-shell structures toward dual-field anti-counterfeiting","authors":"Zhenhua Li ,&nbsp;Qi Han ,&nbsp;Tianpeng Yan ,&nbsp;Jingyu Shang ,&nbsp;Qi Xiao ,&nbsp;Huixin Tong ,&nbsp;Yinglin Song ,&nbsp;Yuxiao Wang ,&nbsp;Xueru Zhang","doi":"10.1016/j.saa.2025.126923","DOIUrl":"10.1016/j.saa.2025.126923","url":null,"abstract":"<div><div>Upconversion luminescent materials exhibit significant potential for optical storage and anti-counterfeiting applications. However, conventional upconversion-encoded materials lack dynamic control over optical states and color modulation, particularly in both visible and NIR modes. Herein, we propose that combining an excellent upconversion luminescent material with photochromic materials provides an efficient strategy for developing a wider variety of photochromic luminescent systems. Novel NaYF₄: Yb, Er @N-TiO₂ core-shell structures were designed to integrate upconversion luminescence and photochromic properties. The core-shell structures exhibit distinct photochromic behavior, demonstrating reversible color changes from white to dark blue under 365 nm irradiation. Using reversible photochromism, the upconversion luminescence intensity can be modulated reversibly with a maximum luminescence modulation rate of 83.4 %. We demonstrated the utility of dual-field stimulus-responsive anti-counterfeiting labels and light-printing applications, where both color and luminescence intensity are recovered via laser thermal treatment. This work establishes a new strategy for designing advanced functional materials for high-security anti-counterfeiting and dynamic information display technologies.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"346 ","pages":"Article 126923"},"PeriodicalIF":4.6,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145118285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancing quinoa(Chenopodium quinoa Willd.) quality assessment using hyperspectral imaging","authors":"Xiaojiang Wang ,&nbsp;Junying Han ,&nbsp;Chengzhong Liu ,&nbsp;Yuming Wei","doi":"10.1016/j.saa.2025.126955","DOIUrl":"10.1016/j.saa.2025.126955","url":null,"abstract":"<div><div>Quinoa, recognized as a nutritionally dense and functionally versatile food source, necessitates non-destructive approaches for quality attribute assessment. This investigation demonstrates enhanced predictive accuracy for three critical quality parameters—protein, starch, and total phenolic content—through spectral information fusion (VIS-NIR and NIR) and spectral feature augmentation, thereby validating the feasibility of employing spectral fusion and enhancement technologies for quinoa quality evaluation. By intensifying characteristic bands closely associated with quinoa quality attributes (identified through Random Frog, Successive Projections Algorithm, and Competitive Adaptive Reweighted Sampling), the spectral model performance was significantly improved(Protein: <span><math><msubsup><mi>R</mi><mi>P</mi><mn>2</mn></msubsup></math></span>=0.9561, RMSEP = 0.1924;Starch:<span><math><msubsup><mi>R</mi><mi>P</mi><mn>2</mn></msubsup></math></span>=0.9692，RMSEP = 0.2175;Total phenols:<span><math><msubsup><mi>R</mi><mi>P</mi><mn>2</mn></msubsup></math></span>=0.9420, RMSEP = 0.0325). Furthermore, an innovative framework integrating explainable artificial intelligence with optimal predictive modeling was developed to enhance quinoa quality assessment. The model's validity and interpretability were substantiated through Shapley Additive exPlanations (SHAP) values. Collectively, the proposed spectral information supplementation methodology and novel model evaluation strategy provide significant insights for quinoa quality attribute detection.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"346 ","pages":"Article 126955"},"PeriodicalIF":4.6,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145103211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An AIE-active triphenylethylene probe for ultrasensitive detection of peroxynitrite and imaging in living cells","authors":"Pu Wang,&nbsp;Su-Hang Xu,&nbsp;Jia-Hao Tang,&nbsp;Zuo-Qin Liang,&nbsp;Chang-Qing Ye","doi":"10.1016/j.saa.2025.126957","DOIUrl":"10.1016/j.saa.2025.126957","url":null,"abstract":"<div><div>Accurate detection of mitochondrial peroxynitrite (ONOO⁻) is crucial for elucidating its roles in physiological and pathological processes. However, its detection remains challenging due to its short half-life, low concentration, and interference from reactive species. To address this, we developed an activatable AIE fluorescent probe, NN-3, which achieves high selectivity through an addition-elimination reaction involving its diphenylphosphinyl group. Upon ONOO⁻-mediated cleavage of the phosphoester bond, the probe exhibits a significant fluorescence enhancement of up to 3062-fold. NN-3 provides a quantitative response to ONOO⁻ over a wide concentration range (5–50 μM), with an ultra-low detection limit of 7.0 nM and rapid response characteristics, while maintaining excellent resistance to biological interference. Furthermore, the probe successfully imaged dynamic ONOO⁻ concentrations in the mitochondria of living cells, exhibiting excellent cell membrane permeability and negligible cytotoxicity.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"346 ","pages":"Article 126957"},"PeriodicalIF":4.6,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145103237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Accurate detection of rice blast using UAV hyperspectral red-edge bands and deep learning method based on cross-attention","authors":"Yuan Qi ,&nbsp;Tan Liu ,&nbsp;Songlin Guo ,&nbsp;Peiyan Wu ,&nbsp;Jun Ma ,&nbsp;Qingyun Yuan ,&nbsp;Weixiang Yao ,&nbsp;Tongyu Xu","doi":"10.1016/j.saa.2025.126939","DOIUrl":"10.1016/j.saa.2025.126939","url":null,"abstract":"<div><div>Rice blast is a fungal disease that threatens rice yield and quality, and timely detection methods are essential to control its spread. Unmanned aerial vehicle (UAV)-based optical Remote Sensing offers a promising approach for monitoring disease in field environments. However, the complex symptoms of rice blast make it challenging to capture the sensitivity of hyperspectral features to disease progression. This study first proposed a collaborative gray wolf optimizer feature selection method with mutual information ranking (MI-CGWO) for characterizing the spectral response of rice blast. Subsequently, the impact of different combinations of canopy red-edge reflectance and fraction vegetation coverage (FVC) on rice blast was evaluated. On this basis, a channel fusion dense cross-attention transformer (CFXFormer) disease monitoring model was developed. The model incorporated a channel interaction fusion module (CIFM) to mitigate modal discrepancies. Additionally, a cross-attention mechanism using Gaussian and self-attention was introduced to capture relationships between fine-grained and coarse-grained data. The results demonstrated that MI-CGWO achieved better classification accuracy with fewer features in a shorter time. Furthermore, vegetation indices (VIs) covering the red-edge and FVC significantly improved model performance compared to models based on conventional VIs, with average OA and Kappa reaching 95.07 % and 93.80 %. Meanwhile, the ideal red edge band may be located at about 701–711 nm and 739 nm. Compared to other classification methods, CFXFormer achieved the best classification performance, with a maximum OA and Kappa of 96.59 % and 95.71 %. Overall, this study offers scientific support for precision agriculture and provides a reference for detecting other crop diseases.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"346 ","pages":"Article 126939"},"PeriodicalIF":4.6,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145155217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A ratiometric fluorescent aptasensor for detection of ACE pesticide through toehold-mediated strand displacement and FRET process","authors":"Zahra Gholami ,&nbsp;Salehe Ganjali ,&nbsp;Mehdi Dadmehr ,&nbsp;Abasali Emamjomeh ,&nbsp;Morteza Hosseini","doi":"10.1016/j.saa.2025.126956","DOIUrl":"10.1016/j.saa.2025.126956","url":null,"abstract":"<div><div>Detection of pesticide residues is important due to their significant environmental side effects and public health concern. Herein, a facile and sensitive approach was developed based on dual emission ratiometric fluorescence signal for detection of acetamiprid (ACE) pesticide. The sensing platform was based on MWCNTs/ AuNPs nanocomposite to generate the output signals. The fluorescent FAM-functionalized aptamer with stem-loop structure was deposited on platform which resulted to fluorescence quenching during FRET process via extinction of MWCNTs/ AuNPs. Upon addition of ACE, aptamer structure was unfolded and specific sequence as the toehold was hybridized with another complementary Cy5 labeled probe. Following, FAM segment was displaced and its fluorescence signal was recovered while the fluorescence emission of Cy5 was quenched by FRET process in proximity of nanocomposite. The biosensor showed efficient detection performance in the linear range between 4 and 40 pM and the LOD was calculated at 2.8 pM. Selectivity of present strategy was confirmed among different applied targets and the real sample assay showed efficient performance in bell pepper samples. Thus, this strategy indicated sensitive and efficient performance for detection of ACE in agricultural product and food stuffs.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"346 ","pages":"Article 126956"},"PeriodicalIF":4.6,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145105891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual − ligand metal − organic frameworks for dual − emission ratiometric and visual detection of hypochlorous acid","authors":"Weiqi Wu,&nbsp;Linlin Wang,&nbsp;Tingting Xiao,&nbsp;Xue−Bo Yin","doi":"10.1016/j.saa.2025.126951","DOIUrl":"10.1016/j.saa.2025.126951","url":null,"abstract":"<div><div>A dual−emission metal−organic framework (UiO − 66 − TCPP) was prepared using 2 − aminoterephthalic acid (BDC − NH₂) and tetrakis(4 − carboxyphenyl) porphyrin (H₂TCPP) as the dual−ligand for ratiometric fluorescent, colorimetric, and visualization triple−mode detection of hypochlorous acid (HClO). Structural and spectroscopic characterization confirmed that the dual−ligand strategy integrated the nanosize of UiO − 66 and the red emission of H₂TCPP, resulting in a stable monodisperse and dual−emission at 465 and 668 nm under single excitation at 390 nm for UiO − 66 − TCPP. Upon exposure to HClO, UiO − 66 − TCPP displayed the enhanced emission at 668 nm, while that at 465 nm increased a little for ratiometric sensing of HClO. Correspondingly, the color transition from yellow to pale pink achieved colorimetric detection of HClO. The detection limits were 0.15 μM for ratiometric sensing and 0.36 μM for colorimetric detection, with a linear range of 5–200 μM for both modes. A test strip was developed for visual assay, allowing on−site fluorescent detection of HClO by RGB analysis with a smartphone. The performance of UiO − 66 − TCPP was confirmed with the recovery testing for water and serum samples. The response mechanism was investigated as the oxidation of pyrrole nitrogen and amino groups, resulting in the multi−modality detection. Thus, we proposed the dual−ligand strategy to realize the nanosize and dual−emission, simultaneously, as a robust platform for sensitive and visible detection of HClO with great potential for environmental and biological monitoring.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"346 ","pages":"Article 126951"},"PeriodicalIF":4.6,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145103258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aggregation-induced emission mechanism-enable fluorescent probes base on benzothiazine for detecting hypochlorous acid in water","authors":"Zhixin Zhang ,&nbsp;Xiangyi Wang ,&nbsp;Xiaoxiao Wu ,&nbsp;Tu Chen ,&nbsp;Jiong Li ,&nbsp;Shijing Shi ,&nbsp;Baolei Fan ,&nbsp;Fei Zhang ,&nbsp;Helin Lu ,&nbsp;Min Li","doi":"10.1016/j.saa.2025.126952","DOIUrl":"10.1016/j.saa.2025.126952","url":null,"abstract":"<div><div>Hypochlorous acid (HClO), as a member of the reactive oxygen species (ROS) family with unique biological properties, plays a sophisticated regulatory role in maintaining organismal homeostasis. In this study, a novel aggregation-induced emission (AIE) fluorescent probe <strong>OEA-BSZ</strong> with “off-on-off” response characteristics was successfully constructed by innovatively using benzothiazine as the molecular framework. This probe exhibits unique dual-mode response behavior: it shows a significant AIE activation effect in the aqueous phase (“off-on” transition), and undergoes fluorescence quenching in the presence of HClO (“on-off “transition). This phenomenon is attributed to the novel AIE mechanism we have first revealed. Moreover, <strong>OEA-BSZ</strong> was successfully applied to the detection of HClO in real water samples by adding a certain amount of standard HClO with known concentration to the samples. This study presents the first application of benzothiazine-based fluorescent probes based on the AIE mechanism for HClO recognition.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"346 ","pages":"Article 126952"},"PeriodicalIF":4.6,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145105892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Isophorone Zn(II) based metal-organic complex fluorescent sensor for monitoring H2O2 and application in real samples, cells, zebrafish and mice","authors":"Ya-Tong Liu ,&nbsp;Han-Wen Cui ,&nbsp;Zhi-Bo Jiang,&nbsp;Si-Yi Yao,&nbsp;Hao Wang,&nbsp;Jing-Yi Li,&nbsp;Yue-Li Zou,&nbsp;Li-Xia Zhao","doi":"10.1016/j.saa.2025.126947","DOIUrl":"10.1016/j.saa.2025.126947","url":null,"abstract":"<div><div>Hydrogen peroxide (H₂O₂) is not only widely used as a biomarker in organisms, but also can be used to effectively remove heavy metal ions, harmful bacteria, and refractory organic compounds in the water environment. Therefore, it is necessary to develop a sensing and visualization tool to monitor the levels of H₂O₂ in organism and water samples. A novel complex <strong>MP</strong> was successfully synthesized based on isophorone, which formed a fluorescent sensor (<strong>Zn</strong>^{<strong>2+</strong>}<strong>-MP</strong>) with Zn²⁺ for swiftly and accurately detecting H₂O₂. Notably, the sensor exhibited rapid, remarkable selectivity, anti – interference ability, and multi - cycle reversibility through “turn–off–on” fluorescence for H₂O₂. Surprisingly, through Job's plot, FT-IR, ESI-HRMS, ¹HNMR titration and theoretical calculations, the possible mechanism by which <strong>MP</strong> formed a complex with Zn²⁺ to achieve the detection of H₂O₂ has been deduced, contained the <strong>Zn</strong>^{<strong>2+</strong>}<strong>-MP</strong> sensor itself exhibited low fluorescence, however when H₂O₂ was added, H₂O₂ competitively captured the <strong>MP</strong> complex in <strong>Zn</strong>^{<strong>2+</strong>}<strong>-MP</strong> sensor, releasing <strong>MP</strong> to enhance fluorescence. Besides, the ability of <strong>Zn</strong>^{<strong>2+</strong>}<strong>-MP</strong> sensor to detect actual water samples (including medicine honeysuckle, soil samples, green tea extract solutions and so on) were tested. Meanwhile, the <strong>Zn</strong>^{<strong>2+</strong>}<strong>-MP</strong> sensor has been successfully achieved visual monitoring in mice, zebrafish and cells. To sum up, the <strong>Zn</strong>^{<strong>2+</strong>}<strong>-MP</strong> sensor held great promise as a highly versatile approach for the rapid and reversible detection of H₂O₂.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"347 ","pages":"Article 126947"},"PeriodicalIF":4.6,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145096676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The organic fluorescent probe for pesticide imazethapyr based on triphenylamine cyanoacrylic acid derivative","authors":"Tao Deng ,&nbsp;Jianhua Lan ,&nbsp;Hongyu Guo ,&nbsp;Xiaoting Fang ,&nbsp;Fafu Yang","doi":"10.1016/j.saa.2025.126950","DOIUrl":"10.1016/j.saa.2025.126950","url":null,"abstract":"<div><div>Imazethapyr is a widely used herbicide but its residue posts a threat to aquatic ecosystems. There is an urgent need to develop a simple, rapid, and in-situ methods for detecting imazethapyr. This study reported the first organic fluorescent probe based on a triphenylamine cyanoacrylic acid derivative (<strong>TPC</strong>) for selective, sensitive and in-situ detection of imazethapyr. Upon binding imazethapyr in aqueous media, <strong>TPC</strong> exhibited a remarkable “turn-on” fluorescence response accompanied by a fluorescence color change from pale yellow to bright green, achieving a low limitation of detection of 4.9 × 10⁻⁸ M. <strong>TPC</strong> demonstrated exceptional sensing selectivity among 16 pesticides and 11 ions, along with pH stability, anti-interference capability, and photostability. The 1:2 binding mechanism was deduced as the multiple hydrogen-bonding interactions and the destruction of intrinsic intramolecular charge transfer (ICT) effect to restore strong AIE emission. Practical applications included test strips and real-sample detection (tap water/mineral water/river water, four soil types, vegetables, and fruits), achieving 94–101.3 % recovery rates. This work demonstrated the excellent potential for simple, rapid and in-situ detection of imazethapyr as an alternative to traditional chromatography in environmental and agricultural monitoring.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"346 ","pages":"Article 126950"},"PeriodicalIF":4.6,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145093372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}