BIO Integration最新文献

{"title":"Editorial note: A Review on Toxicity and Challenges in Transferability of Surface-functionalized Metallic Nanoparticles from Animal Models to Humans","authors":"","doi":"10.15212/bioi-2022-1001","DOIUrl":"https://doi.org/10.15212/bioi-2022-1001","url":null,"abstract":"<jats:p>\u0000 \u0000 </jats:p>","PeriodicalId":431549,"journal":{"name":"BIO Integration","volume":"101 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131684095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combination of Interscalene Brachial Plexus Block with General Anesthesia Attenuates Stress and Inflammatory Response in Arthroscopic Shoulder Surgery","authors":"D. Lin, Zhixiao Han, Yanni Fu, Xiaoqiu Zhu, Jin Li, Hui Xu, Jing Wen, Fei Wang, M. Guo","doi":"10.15212/bioi-2021-0013","DOIUrl":"https://doi.org/10.15212/bioi-2021-0013","url":null,"abstract":"\u0000 In arthroscopic shoulder surgery, general anesthesia (GA) is the common method of anesthesia. Recently, the combined usage of interscalene brachial plexus block with general anesthesia (ISB/GA) was reported to have a lower incidence of adverse side effects than GA alone. However, to date, no study has compared stress and inflammatory responses between these two methods. Since stress and inflammatory responses are critical on intraoperative management and postoperative recovery, we integrated the laboratory and clinical methods and compared the stress and inflammatory factors, such as epinephrine, norepinephrine, glucose, lactate, inflammatory factors tumor necrosis factor a (TNF-a) and interleukin 6 (IL-6), as well as the clinical outcomes to determine whether ISB/GA provides an advantage on stress and inflammatory inhibition. Data showed that ISB/GA resulted in significantly lower epinephrine, norepinephrine, and glucose levels perioperatively. Six hours after operation, the TNF-a and IL-6 levels were also significantly lower in the ISB/GA group. ISB/GA patients had lower blood pressure, a more stable heart rate, lower visual analog scale score, and less opioid consumption during and after surgery. Our results indicate that ISB/GA is a better choice for arthroscopic shoulder surgery, owing to less stress and inflammatory responses during and after operation, which provides better clinical outcomes. Therefore, we recommend ISB/GA as a preferred anesthesia method for arthroscopic shoulder surgery.\u0000","PeriodicalId":431549,"journal":{"name":"BIO Integration","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115681822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Phase I, Randomized, Single-Ascending-Dose, Multiple-Dose, and Food-Effect Trial of the Safety, Efficacy, and Pharmacokinetics of Topiroxostat in Healthy Chinese Participants","authors":"Suiwen Ye, W. Zhuang, Yunni Lin, Xiuping Lai, Junyi Chen, Jing Wang, Guoping Zhong, H. Yao, Junyan Wu","doi":"10.15212/bioi-2022-0006","DOIUrl":"https://doi.org/10.15212/bioi-2022-0006","url":null,"abstract":"\u0000 Background: As the structure of the human diet changes, the prevalence of hyperuricemia is increasing each year. Hyperuricemia and its comorbidities, such as gout, severely affect quality of life. Moreover, hyperuricemia causes renal impairment and is associated with chronic kidney disease. Topiroxostat, a selective xanthine oxidoreductase inhibitor, has been approved to treat hyperuricemia or gout in Japan. Topiroxostat has shown good tolerance and efficacy in the Japanese population. However, its pharmacokinetic (PK) characteristics, efficacy, and safety in the Chinese population remains unknown.Objective: This trial evaluated the PK profile, safety, efficacy, and food effects of Topiroxostat in healthy Chinese participants.Methods: The major endpoint was determination of the PK profile of Topiroxostat. Topiroxostat concentrations were detected with LC-MS/MS. PK parameters were calculated in Phoenix WinNonlin 8.1. Minor endpoints were safety and efficacy assessments. Assessment of adverse events and safety was performed by clinicians. Plasma uric acid concentration (ΔECmax and ΔAUEC) was determined as the pharmacodynamic index. This study consisted of three arms: single ascending dose (20, 40, and 80 mg, N = 10), multiple dose (80 mg BID, 7 days, N = 10), and food effects (40 mg single dose, fasting-fed cross-over design, N = 10).Results: In the single-ascending-dose arm, Topiroxostat showed rapid absorption and excretion, with Tmax <1.6 h and T1/2 2.49–3.72 h. Additionally, Topiroxostat showed a wide distribution, on the basis of moderate Vz/F (242.8–336.36 L). The main PK parameters Cmax, AUC0-t, and AUC0-C showed a linear relationship with dose (R2 = 0.5146, 0.8416, 0.8386, respectively). In the multiple-dose arm, no significant differences were observed in Cmin on days 3–6 (P = 0.265). No serious adverse events were observed. Regarding efficacy, plasma uric acid levels were controlled to low levels during multiple-dose administration. In the food-effects arm, the fed group showed a lower Cmax than the fasting group (316.00 ± 135.81 vs. 478.40 ± 175.42 ng/mL, P = 0.033) but demonstrated better efficacy (ΔECmax, P < 0.001; ΔAUEC, P < 0.001).Conclusions: Topiroxostat showed rapid absorption and a broad distribution in healthy Chinese adults. Additionally, it showed good safety and tolerance in the Chinese population. Moreover, the pharmacodynamic profile indicated that post cibum administration increased the efficacy of Topiroxostat.\u0000","PeriodicalId":431549,"journal":{"name":"BIO Integration","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124533220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation, Characteristics, Differentiation and Exploitation of Human Amnion Mesenchymal Stem Cells","authors":"F. M. Thomas, Sandra Lisa Gumpil, S. F. Simat, V. Subbiah","doi":"10.15212/bioi-2022-0017","DOIUrl":"https://doi.org/10.15212/bioi-2022-0017","url":null,"abstract":"\u0000 Human amnion is a favorable potential source of mesenchymal stem cells (MSC) for future cell-therapy-based clinical applications, because of the painless collection procedure and easy accessibility of the placenta. Human amnion mesenchymal stem cells (hAMCs) have multilineage differentiation ability and high proliferation ability, are non-tumorigenic and have a relatively low risk of rejection after transplantation. Given the beneficial properties of hAMCs, herein, we review the isolation methods and characteristics of hAMCs. Furthermore, we summarize current hAMC applications and preservation methods.\u0000","PeriodicalId":431549,"journal":{"name":"BIO Integration","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116986013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment of Hematological and Serum Biochemical Parameters in 4,834 Healthy Cynomolgus Monkeys (Macaca fascicularis)","authors":"Xue Ling, Ting Luo, Qingyu Huang, Ying He, Longbao Lv, Yun Wang, Ronghua Ma, Jiewen Chen, J. He, Jinrong Zhang, Hongyu Lu, Jian He, Danrong Lin, Siyu Chen, Taiqi Liu, Yan-yan Guo, Z. Liu, Jiaqi Feng, Yudan Mao, Zhiying Chen, Ren Huang, Wen De Li","doi":"10.15212/bioi-2022-0011","DOIUrl":"https://doi.org/10.15212/bioi-2022-0011","url":null,"abstract":"\u0000 Background: In China and other countries, cynomolgus monkeys are commonly used experimental animal models in biomedical research. Reference hematologic and biochemical parameters must be established to evaluate healthy cynomolgus monkeys and investigate drug safety in non-clinical research. In the present study, data from 4,834 healthy cynomolgus monkeys were used to establish a reference for various age groups.Methods: A total of 4834 healthy cynomolgus monkeys (649 males and 4185 females) were selected and divided into six groups according to age and sex. Twenty hematological parameters and 17 serum biochemical parameters were detected, and the effects of age and sex were analyzed.Results: A reference database of hematological and biochemical parameters was established according to age (six groups) and sex (male and female). Significant differences were observed in White blood cell, Red blood cell, Hemoglobin, Hematocrit, Total protein, Albumin, Total bilirubin, Direct bilirubin, Alkaline phosphatase, Glucose, Blood urea nitrogen, Creatinine, Calcium, Total cholesterol, Triglyceride, and Lactate dehydrogenase values according to age in the juvenile and young groups (P<0.05). Significant differences between males and females were observed in Red blood cell, Hemoglobin, Hematocrit, Mean corpuscular hemoglobin concentration, White blood cell, Lymphocyte, Lymphocyte percentage, Monocyte, Monocyte percentage, Basophil, and Basophil percentage in most age groups (P<0.05).Conclusion: Reference ranges for healthy cynomolgus monkeys were established in different age and sex groups in this study. The findings may be useful in clinical care and non-human-primate research.\u0000","PeriodicalId":431549,"journal":{"name":"BIO Integration","volume":"51 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126350123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prognostic Value of Tumor-microenvironment-associated Genes in Ovarian Cancer","authors":"Shimei Li, Jiyi Yao, Shenyan Zhang, Xinchuan Zhou, Xinbao Zhao, N. Di, Shaoyun Hao, Hui Zhi","doi":"10.15212/bioi-2022-0008","DOIUrl":"https://doi.org/10.15212/bioi-2022-0008","url":null,"abstract":"\u0000 Background: Ovarian cancer (OV) is the fifth leading cause of cancer death among women. Growing evidence supports a key role of the tumor microenvironment in the growth, progression, and metastasis of OV. However, the prognostic effects of gene expression signatures associated with the OV microenvironment have not been well established. This study was aimed at applying the Estimation of STromal and Immune cells in MAlignant Tumor tissues using Expression data (ESTIMATE) algorithm to identify tumor-microenvironment-associated genes that predict outcomes in patients with OV.Methods: The gene expression profiles of OV samples were downloaded from The Cancer Genome Atlas database. The immune and stromal scores of 469 OV samples on the basis of the ESTIMATE algorithm were available. To better understand the effects of gene expression signatures associated with the OV microenvironment on prognosis, we categorized these samples into groups with high and low ESTIMATE scores. A different OV cohort from the Gene Expression Omnibus (GEO) database and immunohistochemistry from The Human Protein Atlas database were used for external validation.Results: The molecular subtypes of patients with OV correlated with the stromal scores, and the mesenchymal subtype had the highest stromal scores. Patients with higher stromal scores had lower 5-year overall survival; 449 differentially expressed genes in the stromal score group were identified, 26 of which were significantly associated with poor prognosis in patients with OV (p < 0.05). In another OV cohort from the Gene Expression Omnibus database, six genes were further validated to be significantly associated with poor prognosis. Immunohistochemistry data from The Human Protein Atlas database confirmed the overexpression of CX3CR1, GFPT2, NBL1, TFPI2, and ZFP36 in OV tissues compared with normal tissues.Conclusion: Our findings suggest that CX3CR1, GFPT2, NBL1, TFPI2, and ZFP36 may be promising biomarkers for OV prognosis, with clinical implications for therapeutic strategies.\u0000","PeriodicalId":431549,"journal":{"name":"BIO Integration","volume":"207 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116068141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Voice Series: Interview with Prof. Fuwu Zhang, University of Miami","authors":"Phei Er Saw","doi":"10.15212/bioi-2022-0021","DOIUrl":"https://doi.org/10.15212/bioi-2022-0021","url":null,"abstract":"<jats:p>\u0000 \u0000 </jats:p>","PeriodicalId":431549,"journal":{"name":"BIO Integration","volume":"489 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122898532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hope or hype? Aducanumab as a magic bullet for Alzheimer’s disease","authors":"Wei-Jye Lin, C. Xiao, S. Salton","doi":"10.15212/bioi-2021-0034","DOIUrl":"https://doi.org/10.15212/bioi-2021-0034","url":null,"abstract":"<jats:p>\u0000 \u0000 </jats:p>","PeriodicalId":431549,"journal":{"name":"BIO Integration","volume":"66 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131259057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multilayer Perceptron Predicting Cervical Lymph Node Metastasis for Papillary Thyroid Carcinoma","authors":"Jingwen Shi, Qi Zhang, Tiantong Zhu, Ying Huang","doi":"10.15212/bioi-2021-0029","DOIUrl":"https://doi.org/10.15212/bioi-2021-0029","url":null,"abstract":"\u0000 Background: Lymph node metastasis is related to thyroid cancer recurrence; hence, early identification and prediction of cervical lymph node metastasis (CLNM) in thyroid cancer are essential.Materials and methods: Ultrasound characteristics and patients’ clinical information for 478 thyroid nodules from 383 patients were collected, and a multilayer perceptron (MLP) was used to train and test the veracity to predict CLNM and form a network model. Sixty new patients with papillary thyroid carcinoma (PTC) were evaluated with the MLP neural network model. The metastasis status of these patients was then compared with the pathological results. The prediction of metastasis by the MLP and by multiple regression was compared.Results: Calcification, age, sex, and maximum diameter were important predictive factors of CLNM by the MLP, and the area under the receiver operating characteristic curve was 0.715. No significant differences were found between the MLP and multiple regression in predicting CLNM. The average confidence of the model used in these new patients in predicting metastasis with PTC was 68.9%.Conclusion: Ultrasound images from thyroid nodule characteristics and patients’ clinical information can be used as predictive factors of CLNM by the MLP method to a certain extent.\u0000","PeriodicalId":431549,"journal":{"name":"BIO Integration","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132433138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeted Imaging of Tumor Associated Macrophages in Breast Cancer","authors":"Yanni Xu, Yunting Zeng, Xiaoyun Xiao, Hejun Liu, Boyang Zhou, B. Luo, Phei Er Saw, Q. Jiang","doi":"10.15212/bioi-2022-0010","DOIUrl":"https://doi.org/10.15212/bioi-2022-0010","url":null,"abstract":"\u0000 Breast cancer is a highly heterogeneous disease lacking prognostic markers. Tumor-associated macrophages (TAMs) in the tumor microenvironment are associated with distant metastasis as well as poorer outcomes in breast cancer. Therefore, monitoring TAMs may guide prognostic assessment. This study explores an imaging modality based on a two-step click chemistry procedure for detecting TAMs in breast cancer. Mannose-targeted liposomes (MAN-lipo-AAG) and non-targeted liposomes (lipo-AAG) encapsulating Ac4GalNAz were prepared and comprehensively characterized. The sizes of the prepared MAN-lipo-AAG and lipo-AAG were 126 ± 0.22 and 93 ± 0.23 nm, respectively. In vitro studies demonstrated higher uptake of MAN-lipo-AAG than lipo-AAG by RAW264.7 cells. Moreover, flow cytometry and confocal microscopy confirmed that bright, homogeneous fluorescence labeling was present on RAW264.7 cell membranes in the MAN-lipo-AAG group. Furthermore, in vivo analysis indicated that MAN-lipo-AAG, compared with lipo-AAG, had higher accumulation in a 4T1 xenograft model and higher uptake by mannose-overexpressing TAMs. This study describes a promising approach for specific and non-invasive TAM-targeted imaging via metabolic glycoengineering.\u0000","PeriodicalId":431549,"journal":{"name":"BIO Integration","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133488461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}