BIO Integration最新文献

Low-intensity Pulsed Ultrasound Enhances NK Cell Adoptive Therapy by Modulating the Wnt/β-catenin Signaling Pathway 低强度脉冲超声通过调节Wnt/β-catenin信号通路增强NK细胞过继治疗

BIO Integration Pub Date : 2025-01-01 DOI: 10.15212/bioi-2025-0036

Renjie Feng, Ao Gu, Gökhan Zengin, Meng Du

引用次数: 1

Combating Multi-drug Resistant Mycobacterium Species by Efflux Pump Inhibitors: Contemporary Trends 外排泵抑制剂对抗多药耐药分枝杆菌：当代趋势

BIO Integration Pub Date : 2025-01-01 DOI: 10.15212/bioi-2025-0034

Gautam Kumar, Priya Mondal, Amit Kadam, Isha Dhamija, Ravindra Pal Singh, Deepak Nathiya, Sandeep Kumar

{"title":"Combating Multi-drug Resistant Mycobacterium Species by Efflux Pump Inhibitors: Contemporary Trends","authors":"Gautam Kumar, Priya Mondal, Amit Kadam, Isha Dhamija, Ravindra Pal Singh, Deepak Nathiya, Sandeep Kumar","doi":"10.15212/bioi-2025-0034","DOIUrl":"https://doi.org/10.15212/bioi-2025-0034","url":null,"abstract":"Tuberculosis (TB) is caused by Mycobacterium tuberculosis (M.tb) complex (MTBC), which includes M.tb as one of the causative bacteria. In contrast, non-tuberculous mycobacteria (NTM) refers to Mycobacterium spp. that do not cause TB or leprosy (MTBC spp., M . leprae , and M . lepromatosis ). Mycobacterium spp. are responsible for the deadliest infections and remain a significant challenge in diagnosis and treatment. Mycobacterium spp. have developed multiple complementary mechanisms to defend against antibiotics. Specifically, the mechanisms include modifying the drug target sites, enzymatically inactivating the drugs, and lowering intracellular antibiotic concentrations by overexpressing efflux pumps. These adaptations contribute to the emergence of multi-drug resistant pathogens. This review provides an overview of antibiotic resistance in Mycobacterium spp. with a focus on several key factors, such as enzyme-mediated antibiotic deactivation, gene expression, biofilm formation, and the role of efflux pumps. A critical objective of this review includes Mycobacterium efflux pumps, the significant role in antibiotic resistance, and compounds that act against these efflux pumps.","PeriodicalId":431549,"journal":{"name":"BIO Integration","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.scienceopen.com/document_file/87583d22-cc62-43da-ac04-76dc8afe6cce/ScienceOpen/bioi20250034.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147897425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Ultrasound-Launched Targeted Nanoparticle Enhances Antibacterial Sonodynamic Therapy for Effective Eradication of Pseudomonas aeruginosa Biofilm 超声发射靶向纳米颗粒增强抗菌声动力治疗，有效根除铜绿假单胞菌生物膜

BIO Integration Pub Date : 2024-01-01 DOI: 10.15212/bioi-2024-0001

Lei Xin, Jiaxin Shen, Zixuan Huang, Jifan Chen, Tao Lin, Hongjian Chen, Liuhong Shi, Guowei Wang, Pintong Huang

{"title":"Ultrasound-Launched Targeted Nanoparticle Enhances Antibacterial Sonodynamic Therapy for Effective Eradication of Pseudomonas aeruginosa Biofilm","authors":"Lei Xin, Jiaxin Shen, Zixuan Huang, Jifan Chen, Tao Lin, Hongjian Chen, Liuhong Shi, Guowei Wang, Pintong Huang","doi":"10.15212/bioi-2024-0001","DOIUrl":"https://doi.org/10.15212/bioi-2024-0001","url":null,"abstract":"Abstract Infections caused by Pseudomonas aeruginosa biofilm significantly endanger human health worldwide. Biofilms are closely associated with antibiotic resistance because biofilms significantly undermine the efficacy of antibiotics. A novel ultrasound-launched targeted nanoparticle was developed to universally destroy biofilm, target bacteria, deliver antibiotics, and efficiently kill bacteria via ultrasonic cavitation and antibacterial sonodynamic therapy. The nanoparticle consisted of poly (lactic-co-glycolic acid) loading ciprofloxacin and perfluoropentane with a bacteria-targeted antibody installed on the nanoparticle for binding to specific bacteria. The nanoparticle exhibited a sensitive response to ultrasound and the rapid liquid-gas phase transition of perfluoropentane resulted in a cavitation effect that destroyed the extracellular polymeric substances of the biofilm and allowed deep penetration of the antibiotics. In addition, ciprofloxacin induced additional reactive oxygen species production under ultrasound stimulation, leading to an enhanced bactericidal effect and potent anti-infective activity in vivo . This study presents an effective strategy to tackle the extracellular polymeric substance barriers for overcoming antibiotic resistance and removing a biofilm.","PeriodicalId":431549,"journal":{"name":"BIO Integration","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.scienceopen.com/document_file/6761c6a7-0be9-4c81-a5c6-98a4d48a2067/ScienceOpen/bioi20240001.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147916041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20

The CircHAS2/RPL23/MMP9 Axis Facilitates Brain Tumor Metastasis CircHAS2/RPL23/MMP9轴促进脑肿瘤转移

BIO Integration Pub Date : 2024-01-01 DOI: 10.15212/bioi-2023-0013

Qiang Fu, Haojie Yang, Jingxuan Huang, Fan Liu, Yanni Fu, Phei Er Saw, Yongxin Wang

{"title":"The CircHAS2/RPL23/MMP9 Axis Facilitates Brain Tumor Metastasis","authors":"Qiang Fu, Haojie Yang, Jingxuan Huang, Fan Liu, Yanni Fu, Phei Er Saw, Yongxin Wang","doi":"10.15212/bioi-2023-0013","DOIUrl":"https://doi.org/10.15212/bioi-2023-0013","url":null,"abstract":"Abstract Background: Circular RNAs (circRNAs) regulate tumor development by interacting with microRNAs. However, limited research has been conducted on the roles of circRNAs in gliomas. Therefore, we sought to demonstrate the function and molecular mechanism of circHAS2 in gliomas. Methods: CircHAS2, hsa-miR-508-3p, RPL23, and MMP9 mRNA levels were assessed with qRT-PCR. RPL23 and MMP9 protein levels were determined with western blotting and immunohistochemical staining. Glioma cell migration and invasion were assessed with Transwell assays. The interaction between hsa-miR-508-3p and circHAS2 or RPL23 was predicted with RNAhybrid and miRanda, and confirmed through luciferase reporter assays. The effects of circHAS2 on glioma cells were demonstrated in a nude mouse orthotopic xenograft glioma model. Results: We computationally analyzed the differentially expressed circRNAs in glioma tissues by using the GEO database. The screening indicated that circHAS2 was located primarily in the cytoplasm. Functionally, silencing of circHAS2 inhibited glioma migration and invasion. Mechanically, hsa-miR-508-3p was identified as a downstream target of circHAS2. CircHAS2 was found to regulate RPL23 and influence MMP9 via hsa-miR-508-3p, thereby promoting glioma migration and invasion. Moreover, inhibition of circHAS2 impeded the progression of U87 glioma cells in vivo. Conclusion: CircHAS2 regulates RPL23 and subsequent MMP9 expression by sponging hsa-miR508-3p in glioma cells.","PeriodicalId":431549,"journal":{"name":"BIO Integration","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.scienceopen.com/document_file/b178f60d-9310-4e15-9cf5-a1f951661597/ScienceOpen/bioi20230013.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147895469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Current Applications of Organ-on-a-Chip: A Step Closer to Personalized Medicine 器官芯片的当前应用:离个性化医疗又近了一步

BIO Integration Pub Date : 2022-12-23 DOI: 10.15212/bioi-2022-0027

A. Victorious

引用次数: 2

Nanoparticles Enable Efficient Delivery of Antimicrobial Peptides for the Treatment of Deep Infections 纳米颗粒能够有效地递送抗菌肽用于治疗深部感染

BIO Integration Pub Date : 2021-07-16 DOI: 10.15212/BIOI-2021-0003

Ying-Hu Deng, Rui Huang, Songyin Huang, Menghua Xiong

{"title":"Nanoparticles Enable Efficient Delivery of Antimicrobial Peptides for the Treatment of Deep Infections","authors":"Ying-Hu Deng, Rui Huang, Songyin Huang, Menghua Xiong","doi":"10.15212/BIOI-2021-0003","DOIUrl":"https://doi.org/10.15212/BIOI-2021-0003","url":null,"abstract":"Abstract Antimicrobial peptides (AMPs) have emerged as promising alternatives of traditional antibiotics against drug-resistant bacteria owing to their broad-spectrum antimicrobial properties and low tendency to drug resistance. However, their therapeutic efficacy in vivo,\u0000 especially for infections in deep organs, is limited owing to their systemic toxicity and low bioavailability. Nanoparticles-based delivery systems offer a strategy to increase the therapeutic index of AMPs by preventing proteolysis, increasing the accumulation at infection sites, and reducing\u0000 toxicity. Herein, we will discuss the current progress of using nanoparticles as delivery vehicles for AMPs for the treatment of deep infections.Statement of significanceAntimicrobial peptides (AMPs) are rarely directly used to treat deep infections due to their systemic toxicity\u0000 and low bioavailability. This review summarizes recent progress that researchers employed nanoparticles-based delivery systems to deliver AMPs for the treatment of deep infections. Nanoparticles-based delivery systems offer a strategy to increase the therapeutic index of AMPs by preventing\u0000 proteolysis, increasing the accumulation at infection sites, and reducing toxicity. Especially, the development of intelligent nanocarriers can achieve selective activation and active target in the infectious sites, thus improving the therapeutic efficacy against bacterial infection and reducing\u0000 the toxicity against normal tissues.","PeriodicalId":431549,"journal":{"name":"BIO Integration","volume":"112 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116660495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Voice Series: Interview with Prof. Dr. Sangyong Jon, KAIST Chair Professor : Published Online: January 13 2021 语音系列:采访教授博士Sangyong Jon, KAIST讲座教授:在线发布:2021年1月13日

BIO Integration Pub Date : 2021-07-13 DOI: 10.15212/BIOI-2020-0042

P. Saw

{"title":"Voice Series: Interview with Prof. Dr. Sangyong Jon, KAIST Chair Professor : Published Online: January 13 2021","authors":"P. Saw","doi":"10.15212/BIOI-2020-0042","DOIUrl":"https://doi.org/10.15212/BIOI-2020-0042","url":null,"abstract":"Prof. Sangyong Jon received his Bachelor’s, Master’s and PhD degrees from Korea Advanced Institute of Science and Technology (KAIST). Now, he has returned to KAIST as the Chair Professor at the Department of Biological Sciences and also as the Director of the Center for Precision BioNanomedicine. His research interest lies at the interface of biomedicine, biotechnology, biomaterials, and nanomedicine. His major research focus is on the development of various platform technologies that may address unmet medical needs. Some of the platforms his team developed over a decade ago include the “Aptide” platform that enables the screening and identification of high-affinity peptides for various protein targets such as antibodies; the “Bilirubin Nanomedicine” platform that provides a universal nanomedicine solution for various inflammatory diseases, and the “Cancer Stem Cell” platform that enables facile generation of three-dimensional (3D) cancer stem cell spheroids from various differentiated cancer cells for basic cancer research and drug development. Prof. Jon has received numerous awards and honors including the 2020 KAIST Innovation Prize and the 2015 College of Fellows at the American Institute of Medical Imaging and Biological Engineering. His most recent innovation is the development of bilirubin-based nanoparticles, which has demonstrated its therapeutic feasibility in many pre-clinical disease models, including inflammatory bowel disease. More interestingly, he cofounded a start-up company (BiliX Inc) based on the platform technology. In this interview, we learn first-hand about Prof. Jon’s exciting innovation and the importance of integrating basic science and translational medicine.","PeriodicalId":431549,"journal":{"name":"BIO Integration","volume":"46 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114963922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Voice Series COVID-19 Special Collection Part 1: Interview with clinicians: COVID-19 mutation and current breakthrough in vaccine development 语音系列COVID-19特别集第一部分:临床医生访谈:COVID-19突变和当前疫苗开发的突破

BIO Integration Pub Date : 2021-07-08 DOI: 10.15212/bioi-2021-0008

Phei Er Saw

{"title":"Voice Series COVID-19 Special Collection Part 1: Interview with clinicians: COVID-19 mutation and current breakthrough in vaccine development","authors":"Phei Er Saw","doi":"10.15212/bioi-2021-0008","DOIUrl":"https://doi.org/10.15212/bioi-2021-0008","url":null,"abstract":"As we look back on the rough year of 2020, with the emergence of a novel coronavirus (COVID-19), we were faced with many uncertainties, with no known standard treatments or available vaccines. Scientists and clinicians work around the clock to understand and combat this virus. So far, tremendous progresses have been made especially in genetically identifying this virus, which in turn leads to rapid development of drugs and vaccines. With 3 Food and Drug Administration– and 4 China Food and Drug Administration–approved COVID-19 vaccines so far and many more in the pipelines, we are now faced with the dilemma of weighing the effectiveness of these vaccines versus their side effects. The questions that were asked frequently is: should I get vaccinated? In this two-part interview, BIO Integration first interviewed the team of clinicians who were deeply involved in the writing of “The Guidelines for the Diagnosis, Treatment, Prevention and Control of Coronavirus in Adults in China” during the COVID-19 outbreak. This team, led by Professor Shanping Jiang, along with team member Tiantian Tang, now discusses SARSCoV-2 mutation and progress in vaccine development with BIO Integration.","PeriodicalId":431549,"journal":{"name":"BIO Integration","volume":"48 7","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132624218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Nanocarriers in the Enhancement of Therapeutic Efficacy of Natural Drugs 纳米载体在提高天然药物疗效中的应用

BIO Integration Pub Date : 2021-07-06 DOI: 10.15212/BIOI-2020-0040

Xiuling Li, Shunung Liang, Chee Hwee Tan, Shuwen Cao, Xiaoding Xu, P. Saw, Wei Tao

{"title":"Nanocarriers in the Enhancement of Therapeutic Efficacy of Natural Drugs","authors":"Xiuling Li, Shunung Liang, Chee Hwee Tan, Shuwen Cao, Xiaoding Xu, P. Saw, Wei Tao","doi":"10.15212/BIOI-2020-0040","DOIUrl":"https://doi.org/10.15212/BIOI-2020-0040","url":null,"abstract":"Abstract Since time immemorial, plant derived natural products have been used for the treatment of various human diseases before the intervention of modern medicine. The basis of modern medicine is still being inspired from traditional medicine and therapies. However, despite\u0000 their tremendous therapeutic potential, these natural drugs often have poor bioavailability, metabolic instability, and aqueous insolubility. These factors greatly impede a natural drug’s commercialization potential as a mainstream medicine. Therefore, the development of nanocarrier\u0000 drug delivery systems is indispensable in overcoming the various constraints of the bottlenecks which occur with natural drugs. Of particular interest in this review are four plant materials endogenous to China with the common names of barrenwort or horny goat weed (Epimedium), Shu\u0000 Di Huang (Rehmannia glutinosa, RG), ginseng (Panax ginseng), and Dong Quai or female ginseng (Angelica sinensis, AS), each having been scientifically investigated for a wide range of therapeutic uses as has been originally discovered from the long history of traditional\u0000 usage and anecdotal information by local population groups in Asia. The integration of natural drugs from the East and nanocarrier drug delivery systems developed from the West is paving the way towards further accurate and efficient medicine therapy. We further discuss the potential benefits\u0000 of these plants and the enhancement of their therapeutic efficacy by nanotechnology intervention.","PeriodicalId":431549,"journal":{"name":"BIO Integration","volume":"255 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131665361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

CT Imaging Features of Patients Infected with 2019 Novel Coronavirus 新型冠状病毒感染患者的CT影像学特征

BIO Integration Pub Date : 2021-04-25 DOI: 10.15212/BIOI-2020-0038

Tianhong Yao, Huirong Lin, Jingsong Mao, Shuaidong Huo, G. Liu

引用次数: 1