Jiaxue Liu, Xiaoli Bao, I. Kolesnik, Boyan Jia, Zihan Yu, Caiyun Xing, Jiawen Huang, Tingting Gu, Xiaotong Shao, Alexey Kletskov, A. Kritchenkov, V. Potkin, Wenliang Li
{"title":"Enhancing the in vivo stability of polycation gene carriers by using PEGylated hyaluronic acid as a shielding system","authors":"Jiaxue Liu, Xiaoli Bao, I. Kolesnik, Boyan Jia, Zihan Yu, Caiyun Xing, Jiawen Huang, Tingting Gu, Xiaotong Shao, Alexey Kletskov, A. Kritchenkov, V. Potkin, Wenliang Li","doi":"10.15212/bioi-2021-0033","DOIUrl":null,"url":null,"abstract":"\n To increase the in vivo stability of cationic gene carriers and avoid the adverse effects of their positive charge, we synthesized a new shielding material by conjugating low molecular weight polyethylene glycol (PEG) to a hyaluronic acid (HA) core. The HA-PEG conjugate assembled with the positively charged complex, forming a protective layer through electrostatic interactions. DNA/polyetherimide/HA-PEG (DNA/PEI/HA-PEG) nanoparticles had higher stability than both DNA/polyethyleneimine (DNA/PEI) and DNA/PEI/HA complexes. Furthermore, DNA/PEI/HA-PEG nanoparticles also showed a diminished nonspecific response toward serum proteins in vivo. The in vivo transfection efficiency was also enhanced by the low cytotoxicity and the improved stability; therefore, this material might be promising for use in gene delivery applications.\n","PeriodicalId":431549,"journal":{"name":"BIO Integration","volume":"1 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"15","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"BIO Integration","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.15212/bioi-2021-0033","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 15
Abstract
To increase the in vivo stability of cationic gene carriers and avoid the adverse effects of their positive charge, we synthesized a new shielding material by conjugating low molecular weight polyethylene glycol (PEG) to a hyaluronic acid (HA) core. The HA-PEG conjugate assembled with the positively charged complex, forming a protective layer through electrostatic interactions. DNA/polyetherimide/HA-PEG (DNA/PEI/HA-PEG) nanoparticles had higher stability than both DNA/polyethyleneimine (DNA/PEI) and DNA/PEI/HA complexes. Furthermore, DNA/PEI/HA-PEG nanoparticles also showed a diminished nonspecific response toward serum proteins in vivo. The in vivo transfection efficiency was also enhanced by the low cytotoxicity and the improved stability; therefore, this material might be promising for use in gene delivery applications.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
