Journal of Contemporary Medical Sciences最新文献

{"title":"Effectiveness of radial Extracorporeal Shock Wave Therapy in Reduces muscle spasms in Stroke Patients; A Systematic Review and Meta-Analysis","authors":"Isam Ali Hameedi, A. Shadmehr, T. Alsaadawi","doi":"10.22317/jcms.v9i3.1346","DOIUrl":"https://doi.org/10.22317/jcms.v9i3.1346","url":null,"abstract":"Objectives: Effectiveness of radial extracorporeal shock wave therapy (rESWT) on pain, range of motion and muscle tone in patients with stroke injuries. \u0000Methods: PubMed, EMBASE, Cochrane Library and VIP information were used to collect information for research conducted between the beginning of 2013 and the end of 2022. These studies were randomized controlled trials that used rESWT for muscle spasm in stroke patients with conventional treatments as controls. There are no specific restrictions on the duration of treatment, the type of reESWT, or the severity of symptoms. These studies must have assessed at least one of the following outcome mesurements: visual analog scale (VAS) for pain, Ashwerth measure for muscle tone or external rotation range of motion (ER ROM). RevMan 5.3 software was used to check the quality of included studies. For continuous variables, mean difference (MD) or standardized MD (SMD) with CI 95% were derived. For dichotomous data, event proportions and sample sizes were extracted. \u0000Results: In the conducted investigations, it was found that 7 studies investigated the effectiveness of rESWT  treatments after the intervention in comparison with conventional treatment in terms of reducing pain intensity. In total, all 7 studies showed that the rESWT intervention was effective in reducing pain intensity compared to conventional treatments (MD, –0.97 [95% CI, –2.13 to –0.42], P < .00001, I2 = 71%). Compared with traditional treatments, the effectiveness of rESWT plus routine treatments on muscle tone after intervention was assessed in terms of Ashwerth critrion  in 4 trials (SMD, 1.13 [95% CI, 0.46-1.23], P < .00001, I2 =  59%). ER ROM was used to reflect the ROM, which was assessed in 4 RCTs. Because of the limited number of studies, ER ROM was measured immediately after treatment in all included studies. The pooled result of the included studies showed that the heterogeneity was high and unacceptable (MD, 10.31 [95% CI, 2.47–16.18], P < .003, I2 = 81%). \u0000Conclusion: The results of this research indicated that rESWT treatment can be used as a safe and non-invasive method to quickly reduce spasticity and increase joint range of motion in stroke patients. But more research on the long-term effects of rESWT as well as the factors influencing its effectiveness to reduce spasticity and comparison with other new treatment protocols is suggested.","PeriodicalId":42860,"journal":{"name":"Journal of Contemporary Medical Sciences","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41298706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Demographic and Clinical Profiles Including Comorbidities in Hospitalized Patients Under 18 Years of Age with COVID-19","authors":"A. Memarian, S. Ghasempouri, Mehran Kouchek, B. Sobouti, K. Aghakhani","doi":"10.22317/jcms.v9i3.1340","DOIUrl":"https://doi.org/10.22317/jcms.v9i3.1340","url":null,"abstract":"Objectives: This study was aimed to evaluate the various demographic and clinical characteristics in hospitalized children with COVID-19 as well as their comorbidities.\u0000Methods: This cross-sectional study evaluated a total of 809 hospitalized COVID-19 patients under 18 years of age in the referral university based Ali-Asghar Hospital in 2020. Demographic and clinical characteristics of patients were extracted from the archived records and data analysis was performed using SPSS software version 26.\u0000Results: The mean age of patients was 4.1 years and the higher percentage of patients (57%) were male. The most common symptoms of COVID-19 in children were fever, cough, and diarrhea.  As well, the most common symptoms in 22.1% of patients admitted to the intensive care unit (ICU) were gastrointestinal (GI) symptoms (79%), fever (62.6%), and respiratory distress (53.6%), respectively. The majority of patients were in the age group less than one year (52%) and mortality rate was 6.3% in total and 10% in children with underlying disease. Besides, the mortality rate of intubated cases was 13 times higher.\u0000Conclusion: The findings of present study showed that COVID-19 in children was associated with various clinicopathological manifestations. Underlying disease including respiratory distress, cancer, and kidney disease as well as GI symptoms might be guided predicting the hospitalized cases in ICU. Newborns less than one year of age are exposed to severe COVID-19 infection which is associated with higher mortality rate and it should be given special attention in the early diagnosis and management of the COVID-19 disease.","PeriodicalId":42860,"journal":{"name":"Journal of Contemporary Medical Sciences","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45992973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Adipose Derived Stromal Vascular Fraction on The Early Inflammatory Phase of Burn Wound Healing in Male Rat Model","authors":"Laura Noori, K. Fakiha, A. Aljahdali","doi":"10.22317/jcms.v9i3.1331","DOIUrl":"https://doi.org/10.22317/jcms.v9i3.1331","url":null,"abstract":"Objectives: Severe burn treatment remains challenging. Research has investigated the efficacy of stem cells and stromal vascular fraction (SVF) in many types of injuries. SVF has advantages for its heterogenous population of cells and avoidance of culturing and ethical issues. SVF can be isolated either enzymatically or mechanically. Enzymatically isolated SVF reduced inflammation and enhanced neovascularization and re-epithelization in the treatment of burn injury. This study highlighted the efficacy of applying mechanically isolated SVF on the early inflammatory phase of deep partial-thickness burn by investigating toll-like receptor 4 (TLR4) signaling pathway. \u0000Methods: 30 male Wistar rats with deep partial-thickness burns were assigned into: the control group, silver sulfadiazine (SSD) cream group, and mechanically isolated SVF group. Morphological, histopathological evaluation of inflammation, and immunohistochemical analysis for (TLR4, TNF-α, IL‐1β, and IL-6) were evaluated for the first eight days post-treatment. \u0000Results: Morphologically, the SVF group significantly reduced edema and increased wound bed dryness on day one compared to the control group (P=0.0001) and to the SSD group (P=0.001). Histopathologically, the SVF group significantly reduced inflammation four days post-treatment compared with the control group (P=0.045). The SVF group significantly reduced TLR4 protein expression on days four and eight post-treatment compared with the control group (P= 0.001, P= 0.042, respectively). The TNF-α protein expression in the SVF group was significantly lower on days four and eight post-treatment compared with the control group (P= 0.046, P= 0.046, respectively) and with the SSD group (P= 0.008, P= 0.001, respectively). The IL‐1β expression was significantly reduced in the SVF group compared to the control group on day four post-treatment (P= 0.017). There were no significant differences in IL-6 expressions between all groups on both days. \u0000Conclusion: The mechanical isolation of SVF has an early anti-inflammatory impact on deep partial-thickness burn injury. This effect could be through inhibiting TLR4, TNF-α, and IL‐1β pathways. This could partially explain the mechanism behind SVF efficacy in the healing process of burn injury.","PeriodicalId":42860,"journal":{"name":"Journal of Contemporary Medical Sciences","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46424122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dosimetric effects and impacts caused by a carbon fiber table and its accessories in a linear accelerator","authors":"H. Alabedi, M. S. Al Musawi, Nabaa Mohammed Ali","doi":"10.22317/jcms.v9i3.1355","DOIUrl":"https://doi.org/10.22317/jcms.v9i3.1355","url":null,"abstract":"Objective : Most contemporary treatment planning systems (TPS) exclude the couch during treatment planning. The present investigation aimed to evaluate the impact of couches and accessories on radiation therapy planning by quantifying the degree of attenuation for two-photon beam energies, 6 MV and 10 MV, at two different field sizes, 5 cm x 5 cm and 10 cm x 10 cm.   \u0000Methods: An x-ray radiation beam at two energies (6 MV and 10 MV) generated by a linear accelerator (Elekta Synergy). The output dose was measured using a digital parallel plate ionization chamber of the IBA cc13 type (IBA Dosimetry, Germany), and the readings were recorded with a 2944 Farmer Type chamber. Different commercially available couch tops and accessories evaluated in this study. \u0000Results: The results show that the highest attenuation values were observed at 1300 gantry angles for both energies and field sizes. The lowest attenuation values were recorded at 1000 gantry angles with 6 MV energy for 5cm x 5cm and 10cm x 10cm field sizes. The breast board with D level demonstrated the highest attenuation of 6.27% and 5.51% for 6 MV and 10 MV energies, respectively. \u0000Conclusion: the findings indicate that the degree of attenuation is not uniform across all angles and may exceed tolerable limits, indicating the need for careful consideration during treatment planning to ensure optimal treatment outcomes.","PeriodicalId":42860,"journal":{"name":"Journal of Contemporary Medical Sciences","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41749843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular detection of mononucleotide biomarkers of microsatellite instability in sporadic colorectal carcinoma patients with clinicopathological correlation","authors":"Wed Thamer Salman Al-Jumaili, Bassam M S Al-Musawi","doi":"10.22317/jcms.v9i3.1348","DOIUrl":"https://doi.org/10.22317/jcms.v9i3.1348","url":null,"abstract":"Objectives: To identify the frequency and types of microsatellite instability among a group of sporadic CRC patients and to correlate the findings with clinicopathological characteristics. \u0000Methods: During an 8-month period, all patients with sporadic CRC who attended to two teaching hospitals in Baghdad, Iraq were recruited to this cross-sectional study regardless of age, sex, ethnicity, or tumor characteristics. Demographic, clinical, and histopathological features were recorded. DNA was extracted from FFPE-blocks of the resected tumors and normal tissues. PCR amplification of five microsatellite mononucleotide repeat loci (BAT25, BAT26, NR-21, NR-24, and MONO-27) and 2 pentanucleotide repeat control markers (Penta C and Penta D) was performed to determine the MSI status. Capillary electrophoresis and Genetic Analyzer 3500 (Applied Biosystem, Japan) were used to separate and examine the products. Data were analyzed by Genescan software (Promega, USA). Instability of two or more loci is considered MSI-H. \u0000Result: In this study, ages of the 45 recruited patients ranged between 20-80 years, with a mean±SD of 55±12.3 years; of them, 31(68.9%) were ≥50 years; 25 (55.6%) were males. Rectal bleeding was the most frequent presenting feature [22 (48.9%)] patients; 23 (51.1%) of CRCs were located at recto-sigmoid region, 29 (64.4%) were T3 tumors, 34(75.5%) were non-mucinous adenocarcinoma, 39(86.7%) were moderately differentiated, 17 (37.8%) patients had stage III tumors; and 25 (55.5%) had lymphovascular invasion. MSI-H was seen in 5/45 (11.1%) patients; 3(60%) of them were ≥50 years, 4(80%) were males, 3(60%) were smokers, 2 (40%) presented with intestinal obstruction and altered bowel habits each; 4(80%) had T3 tumors, 3(60%) had mucinous adenocarcinomas [p=0.004], 2(40%) had stage II tumor and stage III each. \u0000Conclusion: The frequency of MSI-H among the recruited patients with CRC was 5/45 (11.1%) and it was significantly associated with mucinous adenocarcinoma subtype. NR-24 and NR-21 were the most prevalent instable markers.","PeriodicalId":42860,"journal":{"name":"Journal of Contemporary Medical Sciences","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45703340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of Neuropsychological assessment in the Differential Diagnoses of Late-Onset Depression, Dementia, and Mild Cognitive Impairment","authors":"G. Morkavuk, Gokce Sirvanli","doi":"10.22317/jcms.v9i3.1233","DOIUrl":"https://doi.org/10.22317/jcms.v9i3.1233","url":null,"abstract":"Objective: The present study aimed to compare the neuropsychological test parameters of dementia, depression, and MCI patients and determine the disease-specific test characteristics and their relationship with electroencephalography.\u0000Methods: Ninety-one patients who were admitted to the neurology outpatient clinic with forgetfulness complaints between October 2019 and March 2022 and whose neuropsychological tests were performed were included in the study. The files of these 91 patients were reviewed retrospectively and their sociodemographic data were recorded. Furthermore, the EEG results which were taken during the patients' evaluation period due to forgetfulness were evaluated. Neuropsychological tests were compared between patients with dementia, MCI and depression. It was also investigated whether there was a relationship between NPT test parameters and EEG in patients with EEG results.\u0000Results: The study was completed with 87 patients. Of these 87 patients, 54 were female and 33 were male. Twenty-four patients had depression, 16 MCI, and 47 dementia. All of the dementia patients had Alzheimer's type dementia. When dementia, depression, and MCI groups were compared, the age difference was statistically significant (p=0.001). The mean age of the depression group was 66.5, the MCI group was 73.5, and the dementia group was 77. WMS-I, WMS-II, WMS-III, WMS-IV, similarities test, clock drawing test, trail making test, shape copying, language, and mood evaluation tests were statistically significantly different between the groups.  There was no statistically significant difference between the groups regarding gender, education level, dominant hand, and occupation. EEG background activity frequencies were also examined between the groups, and there was no statistically significant difference.\u0000Conclusion: In conclusion, when evaluating patients who present with the complaint of forgetfulness, a detailed neuropsychological evaluation must be performed in addition to other diagnostic tests. Sensitive tests should be included to confirm the diagnosis, especially in cases where being in between for the differential diagnosis. Thus, further studies are needed on this subject.","PeriodicalId":42860,"journal":{"name":"Journal of Contemporary Medical Sciences","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42296911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Case report: Identification of a rare homozygous missense variant in the PKLR gene reported for the first time in transfusion-dependent Saudi Patient","authors":"Rawabi Zahed, B. Sajer","doi":"10.22317/jcms.v9i3.1354","DOIUrl":"https://doi.org/10.22317/jcms.v9i3.1354","url":null,"abstract":"Red cell pyruvate kinase deficiency is one of the most common erythrocytic glycolytic pathway defects connected with congenital non-spherocytic anemia. The condition inherited as an autosomal recessive Mendelian trait is caused by mutations in the PKLR gene located on chromosome 1q21. Pyruvate kinase enzyme is crucial in the energy-producing glycolysis pathway that provides red blood cells with the primary source of energy (ATP). We report here a case of a Saudi female patient that was initially diagnosed at a few months old with beta-thalassemia major and was treated with regular blood transfusions and iron overload management. At the time of our sample collection, the patient was recently transferred to King Abdul Aziz University Hospital. Genetic testing was performed to identify the disease-contributing variant of beta-thalassemia using TaqMan genotyping of six common beta-thalassemia variants (negative results). NGS targeted HBB gene sequencing which did not reveal any related variants. MLPA was performed to rule out alpha thalassemia diagnosis. The use of whole genome sequencing revealed a rare missense variant in the PKLR gene c.1015G>A (D339N) in a homozygous state that correlates to her severe phenotype. Documenting this incident will aid medical staff in providing appropriate care to similar cases and highlights the importance of following up with the diagnosis investigation process to minimize misdiagnosis incidences.","PeriodicalId":42860,"journal":{"name":"Journal of Contemporary Medical Sciences","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47930982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic polymorphisms of DPYD in patients with breast cancer on capecitabine therapy","authors":"Raaid Fadhl Abbas, A. Sahib, Hasanain Shakir Mahmood, Karar Kadhim Mohsin, Ali Amal Aldeen Majeed","doi":"10.22317/jcms.v9i3.1350","DOIUrl":"https://doi.org/10.22317/jcms.v9i3.1350","url":null,"abstract":" Objectives:   Breast cancer is the primary cause of death in Iraqi women aged 30–54 years. The study examined the relationship between (G > A) (rs3918290) and (rs55886062, T >G) DPYD gene polymorphisms, their haplotypes, and capecitabine serum concentrations in postmenopausal Iraqi women with breast cancer in postmenopausal women. During capecitabine chemotherapy. \u0000Methods: The study included 200 women: 100 apparently health (45-75 years old) and 100 with breast cancer (40-70 years old). This study, conducted between July and October 2022 at the oncology centre at Imam al-Hussain medical city in Kerbala, Iraq, plasma levels of Capacetabine and 5fu were measured in  breast cancer patients who had been taking capecitabine for at least three months. All participants gave informed consent. \u0000Results: Capecitabine, and 5FU concentrations in breast cancer patients differed significantly, As the results showed, Capecitabine, and 5FU had a significantly higher concentration of them in patients with the TT allele than in those with the CC and CT alleles for the polymorphism (IVS14 + 1G > A) (rs3918290) and in patients with DPYD*13 (rs55886062) with the CC allele rather than the AA and AC alleles. Mutant allele carriers had increased Capecitabine concentrations (p<0.001). \u0000Conclusion: Ca15.3, serum calcium, and estradiol all exist in bodily serum, making them a potentially useful novel diagnostic biomarker for patients with breast cancer due to their high levels of stability, as well as the biological properties of tumors, such as serum calcium and estrogen.","PeriodicalId":42860,"journal":{"name":"Journal of Contemporary Medical Sciences","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49105610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation, identification and comparative analysis of 16S rRNA of Multidrug-resistant Bacteria Clinically isolated from Al Qassim Region Hospitals in Saudi Arabia","authors":"H.S. Alkhowaiter, Othman Alyahyawy, Abdullah Abdulhafeez Aljeddawi, Salah El-Deen Mohamed Abo-Aba","doi":"10.22317/jcms.v9i2.1337","DOIUrl":"https://doi.org/10.22317/jcms.v9i2.1337","url":null,"abstract":"Objective:   This study concluded that the antibiotic resistance and gene transfer across bacterial strains in the hospital setting are two possible explanations for the observed sequence changes in the target microorganisms. \u0000Methods:   The 16S rDNA genes of all isolates were effectively amplified using PCR, and detailed identification results were derived from GenBank databases. The BLAST search resulted in the classification of 28 isolates into five strains. The GC content of bacterial sequences varies greatly between single species. \u0000Results:  16S rDNA was utilized to identify bacterial species from isolates strains results demonstrated that identification of bacterial strains. Sequences varied between and within strains, also, variation are noticed in genomic nucleotide content of isolated and identified strains. Results of the present study demonstrated that the observed heterogeneity in the sequences of our target bacterial strains may be linked to antibiotic resistance and gene transfer between bacteria that evolved as a result of the hospital environment. \u0000Conclusion:  This study concluded that antibiotic-resistant bacteria are capable of transferring copies of their DNA encoding a resistance mechanism to other bacteria, even those that are distantly related to them. the observed heterogeneity in the sequences of our target bacterial strains may be linked to antibiotic resistance and gene transfer between bacteria that evolved as a result of the hospital environment.","PeriodicalId":42860,"journal":{"name":"Journal of Contemporary Medical Sciences","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41635771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A potential role of extracellular DNA in biofilm and ciprofloxacin resistance","authors":"Hind Tahseen Ibrahim, Ali A. Mussa, Harith Jabbar Fahad Al-Mathkhury","doi":"10.22317/jcms.v9i2.1338","DOIUrl":"https://doi.org/10.22317/jcms.v9i2.1338","url":null,"abstract":"Objectives: This study aims to broaden our knowledge of the role of eDNA in bacterial biofilms and antibiotic-resistance gene transfer among isolates. \u0000Methods: Staphylococcus aureus, E. coli, and Pseudomonas aeruginosa were isolated from different non-repeated 170 specimens. The bacterial isolates were identified using morphological and molecular methods. Different concentrations of genomic DNA were tested for their potential role in biofilms formed by study isolates employing microtiter plate assay. Ciprofloxacin resistance was identified by detecting a mutation in gyrA and parC. \u0000Results: The biofilm intensity significantly decreased (P < 0.05) concerning S. aureus isolates and insignificantly (P > 0.05) concerning E. coli isolates. Yet, one E. coli isolate's biofilm was significantly decreased (P < 0.05) linearly with increasing eDNA. Of considerable interest, the addition of eDNA led to a significant increase (P < 0.05) in the biofilm of the two-tested P. aeruginosa isolates. Moreover, eDNA participated in transferring Ciprofloxacin resistance to the sensitive isolate when it presents in its biofilm. \u0000Conclusion: eDNA has a dual effect on bacterial biofilms either supportive or suppressive following bacterial species per se. Also, it seems to play an important role in antibiotic resistance within the biofilm.","PeriodicalId":42860,"journal":{"name":"Journal of Contemporary Medical Sciences","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43602588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}