{"title":"Bioprospecting of Lactobacillus sp. Starter Culture from Colostrum Breast Milk for Probiotic Milk Production from Soybeans","authors":"Mufti Hatur Rahmah, Masriany Masriany, Ramlah Ramlah, Isdaryanti Isdaryanti","doi":"10.36987/jpbn.v9i2.4427","DOIUrl":"https://doi.org/10.36987/jpbn.v9i2.4427","url":null,"abstract":"Lactobacillus sp. is a bacterium that naturally exists in various environments, including in breast milk colostrum. This bacterium belongs to a group of lactic acid bacteria that produce lactic acid as the main product of sugar fermentation so that it has probiotic properties that also play a role in the fermentation process. This study aims to produce probiotic milk using Lactobacillus sp isolates from breast milk colostrum with soybean (Glycine max (L.) Merril.) as the base material and then test the total lactic acid content, pH value, reducing sugar and the number of lactic acid bacteria. The production of soybean fermented milk is based on the analysis of total lactic acid content by acid-base titration method with 0.1 N NaOH standard solution, pH value by using universal pH meter, reducing sugar test by spectrophotometric method of Nelson-Somogyi and the number of lactic acid bacteria by using Glucose Yeast Pepton Agar (GYPA) medium added with CaCO3 then calculated by using Standard Plate Count (SPC) method with TPC value (Total Plate Number) is 2.3. 1012 colonies/ml, 2.5.1012 colonies/ml, 4.5.1014 colonies/ml, 4.1.1014 colonies/ml. From the results of the study it is known that Lactobacillus sp isolates from breast milk colostrum fluid can ferment soybean milk (Glycine max (L.) Merril.)","PeriodicalId":422054,"journal":{"name":"JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS","volume":"83 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124798958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Length – Weight Relationship Analysis and Condition Factor Blood Clams (Anadara granosa) In Panipahan, Rokan Hilir Regency, Riau Province","authors":"Manja Asmita, R. Machrizal","doi":"10.36987/jpbn.v9i2.4386","DOIUrl":"https://doi.org/10.36987/jpbn.v9i2.4386","url":null,"abstract":"This research was conducted in the Rokan Hilir district with the aim of analyzing the length-weight relationship and condition factors of blood clams (Anadara granosa). The benefit of this research is to provide information about the length of weight and condition factors of A. granosa which can be used as a reference in cultivation and further research. This research was carried out for 90 days from October to December 2021, in the waters of Panipahan, Rokan Hilir Regency. This length-weight relationship was calculated using a linear allometric model (LAM), while the condition factor was calculated using the Fulton formula (K) and relative weight (Wr). The results of the analysis of the length-weight relationship of blood clams get a value of b <3 = 0.894, which is a negative allometric where the increase in length is faster than the growth in weight. With the value of relative weight (Wr) 71,325-149,883 and Fulton’s condition factor (K) 75,372-387,106. This is caused by environmental conditions that are quite good in supporting blood clams (A. granosa) . in the waters of Panipahan Kab. Rokan Hilir","PeriodicalId":422054,"journal":{"name":"JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS","volume":" 29","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132075141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F. Harahap, Kenari Br Sinuraya, S. Syarifuddin, C. Suriani, Ayu Putri Ningsih, Syahmi Edi, Nusyirwan Nusyirwan
{"title":"The Effect of IAA and BAP on Root Induction of Cattleya Orchids","authors":"F. Harahap, Kenari Br Sinuraya, S. Syarifuddin, C. Suriani, Ayu Putri Ningsih, Syahmi Edi, Nusyirwan Nusyirwan","doi":"10.36987/jpbn.v9i2.4481","DOIUrl":"https://doi.org/10.36987/jpbn.v9i2.4481","url":null,"abstract":"Orchid is an ornamental plant commodity that has an important meaning in international trade. Until now, orchids are still the center of attention of farmers and lovers of ornamental plants, because of their bright potential as cut flowers and potted plants. The popular and widespread type of orchid is Cattleya sp. The Cattleya orchid is also called the queen of orchids because of its colorful flowers. Cattleya sp. orchids take a long time to propagate by seed, about 4 to 7 years, so other methods are needed to deal with them. Tissue culture is a method for isolating plant parts such as cells, tissues or organs, and cultivating them in an aseptic environment. The purpose of this study was to determine the optimal interaction of growth regulators indole acetic acid (IAA), benzyl amino purine (BAP), and their interactions with the root induction of Cattleya sp. Cytokinins interact with auxin in determining the direction of cell differentiation. In this research method, differences in concentrations of indole acetic acid (IAA: 0, 2, 4, 6 ppm) and concentrations of benzyl amino purine (BAP: 0, 0, 5, 1 ppm) were carried out. Parameters observed were the number of roots and root length. Data were analyzed descriptively and inferentially using ANOVA. The highest number of roots was obtained in the treatment medium containing 0.5 ppm BAP and 6 ppm IAA, which was 6.33. The highest root length was found in the B0I4 treatment (BAP 0 + IAA 4 ppm). The results of the analysis of the interaction variance between the IAA treatment and the BAP treatment were 0.005, meaning that there was a significant effect of the combination of IAA and BAP on the leaf length of Cattleya sp.","PeriodicalId":422054,"journal":{"name":"JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS","volume":"77 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117119033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Morphometric Study of African Spurred Tortoise (Centrochelys sulcata) Based on Age Group","authors":"Tetty Barunawati Siagian, Muhammad Firman Bayu Prayogo, Danuja Widigdaya, Pungki Pungki","doi":"10.36987/jpbn.v9i2.4344","DOIUrl":"https://doi.org/10.36987/jpbn.v9i2.4344","url":null,"abstract":"Centrochelys sulcata are kept and bred to prevent extinction. The sulkata tortoises were identified to determine their respective identities by measuring them morphometrically. Morphometric studies on sulkata tortoises are still minimal, so a morphometric study of the sulkata tortoises is needed. The purpose of this study was to identify and describe the morphometry of sulcata tortoises with three different age ranges kept in ex-situ conservation. The research sample used 15 sulcata turtles which were divided into 3 groups namely group A (1 year), Group B (2 years), and Group C (3 years). The morphometric parameters measured were body weight, head, carapace, plastron, forelimb, hindlimb and bridge. The results showed that the average body weight of group A, B and C turtles was 2,179 kg, 1,289 kg and 0,167 kg respectively. The average maximum carapace length in a row for groups A, B, C, namely 11.64, 23.18 and 16.72 cm. The average plastron lengths for groups A, B, C were 9.08, 6.44 and 9.24 cm respectively. The average forelimb length in groups A, B and C were 3.04, 6.28 and 7.78 cm respectively. The average hindlimb length for groups A, B, and C were 1.86, 3.5 and 4.04 cm, respectively. The average bridge width for groups A, B and C were 4.36, 8.94 and 10.1 cm respectively. Based on the results of morphometric measurements of the three groups of sulcata tortoises, it was shown that group C had a larger morphometric size than groups B and A, this was due to differences in age and growth factors. Sulcata tortoises group C is 3 years old and belongs to the juvenile group. Sulcata tortoise groups A and B aged 1 and 2 years are included in the yearly group","PeriodicalId":422054,"journal":{"name":"JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133561094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Development of Scratch Software-Based Interactive Learning Media on Regulatory System Material","authors":"Nurhalizah Nurhalizah, U. N. A. D. Jayanti","doi":"10.36987/jpbn.v9i2.4462","DOIUrl":"https://doi.org/10.36987/jpbn.v9i2.4462","url":null,"abstract":"The research aims to develop interactive learning media based on software scratch on the material of the regulatory system and to know the validity, practicality, and efficacy of the media. The research and development method with the ADDIE model is used in this research. The research instruments used are needs analysis in the form of teacher interview guides and student questionnaires, validation sheets consisting of media and materials expert validation sheets, and implementation tools in the form of effectiveness sheets, namely pre-test and post-test, and practicality sheets, namely teacher and student response questionnaires. Based on the results and discussion, the media expert`s validity test result is 87.33%, and the material expert's result is 87.50%, indicating that it is very valid. Then, the practicality test results of the teacher's responses received a percentage of 97.50%, which is very practical. The practicality test of student responses on a small scale yielded a rate of 93.25%, which is very practical, while the field test yielded a rate of 84.30%, which is quite practical. In the effectiveness test, the percentage of n-gain obtained was 84%, which means it was effective. The results show that scratch-based interactive learning media have proven to be valid, practical, and effective. This learning media can be implied as one of the supporting media for teachers in biology subjects, particularly on regulatory system material that may boost students' motivation for learning and academic success.","PeriodicalId":422054,"journal":{"name":"JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130192746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Development of Student Worksheets Based on Science Process Skills on Human Excretion System Materials","authors":"Erma Lisa, Ira Suryani, Efrida Pima Sari Tambunan","doi":"10.36987/jpbn.v9i2.4285","DOIUrl":"https://doi.org/10.36987/jpbn.v9i2.4285","url":null,"abstract":"Development of Student Worksheets (LKPD) based on science process skills on human excretory system material presented in the form of an application on a smartphone which contains human excretion system material, stages of practicum activities and questions adapted to indicators of science process skills, which are expected to be able to train participants' thinking abilities educate. The research aims to find out: (1) the feasibility of developing science process skills-based LKPD (Student Worksheets): (2) the practicality of developing process skills-based LKPD (Student Worksheets); and (3) The effectiveness of developing LKPD (Student Worksheets) based on science process skills on human excretory system material. The development model in this study is 4D which consists of 4 stages, namely define, design, development, and disseminate the research subject of 30 students of class XI IPA MAS 12 Perbaungan. The research instruments are validation sheets for material experts, linguists, and media experts on feasibility testing, practitioner validation sheets to determine practicality, pretest and posttest sheets for effectiveness testing. The results of this study prove that the skill-based LKPD science process developed is declared very feasible with a percentage of 89.39%, very practical with a percentage of 81.94% and very effective with a high category of learning improvement that has been tested with n-gain with a score of 0.73 and excellent student response with a percentage of 81.58%. The results of this study prove that the developed science process skills-based learner worksheets are very feasible, very practical, and very effective to use in the learning process","PeriodicalId":422054,"journal":{"name":"JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS","volume":"87 12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127991758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Juswardi Juswardi, Rina Yuliana, Nina Tanzerina, Harmida Harmida, N. Aminasih
{"title":"Anthocyanin, Antioxidant and Metabolite Content of Butterfly Pea Flower (Clitoria ternatea L.) Based on Flowering Phase","authors":"Juswardi Juswardi, Rina Yuliana, Nina Tanzerina, Harmida Harmida, N. Aminasih","doi":"10.36987/jpbn.v9i2.4064","DOIUrl":"https://doi.org/10.36987/jpbn.v9i2.4064","url":null,"abstract":"The butterfly flowers (Clitoria ternatea L.) are used as natural dyes and herbal medicines because they contain metabolites, anthocyanins and antioxidants. The content of these metabolites is influenced by the phase of flower development. This study aims to determine the levels of anthocyanins, antioxidants and metabolites of butterfly pea flowers from different flowering phases. The Butterfly flower collection was taken from the village of Mulyaguna, Teluk Gelam, Ogan Komering Ilir, South Sumatra. The extraction was carried out with 70% ethanol solvent, and determination of the amount of anthocyanin content by spectrophotometry, antioxidant content by DPPH method, and metabolite compounds by GC-MS. Data on anthocyanin and antioxidant content were analyzed with averages and standard deviations, and GC-MS chromatograms were traced for compounds with reference to the PubChem, KEGG, ChEBI, PlantCyc, and Spectrabase websites, which then determined the dominant compound group. The results of the study on blooming butterfly pea flowers found that the antioxidant content was 6.58 ppm, higher than that of bud flowers, which were 2.55 ppm, and wither flowers, which were 1.74 ppm. The anthocyanin content of the blooming butterfly pea flower was 40.33 ppm, the withering flower was 4.36 ppm, and the bud flower was 3.60 ppm. The dominant metabolites were identified as fatty acids, organic acids, aromatics and flavanoids, followed by differences in antioxidant and anthocyanin content in the flowering phase of the butterfly pea flower","PeriodicalId":422054,"journal":{"name":"JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS","volume":"36 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131972930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. Lestari, Eliza Febrianti, Shahnaz Sabrina Wulansari, A. Putra
{"title":"Analysis of Liver Histology Several Fish in the Air Kotok River in the Gold Mining Area, Lebong Regency","authors":"D. Lestari, Eliza Febrianti, Shahnaz Sabrina Wulansari, A. Putra","doi":"10.36987/jpbn.v9i2.4020","DOIUrl":"https://doi.org/10.36987/jpbn.v9i2.4020","url":null,"abstract":"Fish have habitats that are easily exposed to contamination from the environment such as heavy metal waste which can cause changes in the structure and function of body tissues. One type of waste that enters the river in the Lebong Regency River is gold mining processing waste that uses mercury and cyanide. Heavy metals can enter organisms and accumulation and biomagnification of mercury can occur in the bodies of aquatic biota such as fish. There has been no research related to fish histology in the Lebong gold mining area, so the aim of this research was to determine the histological condition of the liver organs of several fish in the form of Hemibagrus nemurus, Rasbora sp, Trichogaster trichopterus, and Macrognathus circumcinctus in the Air Kotok river in the gold mining area of Lebong Regency. The research was conducted using the paraffin method with hematoxylin-eosin staining. Data were analyzed descriptively qualitatively based on the histology of the fish liver. The results of the research on the liver histology, it shows damage to the fish liver structure which includes vacuolization, congestion, pyknosis, dilated sinusoids, melanomacrophages and leukocyte infiltration. The damage to the hepatocytes of this fish can be used as a bio-indicator of exposure to toxic substances in the Air Kotok river, one of which is heavy metal activity from gold mining, which can be a threat to the health and survival of the fish.Keywords: Bengkulu, gold mining waste, heavy metal, hepa","PeriodicalId":422054,"journal":{"name":"JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131291240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Abundance and Diversity of Polychaete Worms in Kapuas Estuary, West Kalimantan","authors":"Junardi Junardi, Riyandi Riyandi","doi":"10.36987/jpbn.v9i2.4003","DOIUrl":"https://doi.org/10.36987/jpbn.v9i2.4003","url":null,"abstract":"Estuaries provide valuable ecosystem services, but they are also subject to environmental pressures due to high anthropogenic activities. The diversity and abundance of Polychaete can be used as indicators of the health of the Kapuas estuary, but supporting data are currently lacking. The objective of this research is to assess the quality of the Kapuas estuary through a Polychaete community approach. The study employed a survey method by selecting ten Polychaete sampling points based on their surrounding environmental conditions. Samples were collected from each point using a Petite Ponar grab, filtered, sorted, and grouped by family. The Polychaete specimens were identified up to the genus level. The data were analyzed descriptively. A total of 108 Polychaete individuals were found, comprising nine genera and seven families. The highest abundance was observed in the genera Capitella (Capitellidae) and Sternaspis (Sternaspidae), with 778 ind.m-2and 667 ind.m-2, respectively. The diversity of Polychaete in the Kapuas estuary was classified as low, with diversity index ranging from 0.5 to 1.21. The high abundance, low diversity, and presence of indicator genera of organic pollutants such as Capitella indicate that the Kapuas estuary is affected by organic contamination","PeriodicalId":422054,"journal":{"name":"JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124127272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Plant Effectiveness of Acorus calamus, Pistia stratiotes, Azolla pinnata as Hyperaccumulator Candidate of Phytoremediation Agent for Copper (Cu) Absorption","authors":"Juriyah Astika Dewi, I. Nurwahyuni, E. Munir","doi":"10.36987/jpbn.v9i2.4291","DOIUrl":"https://doi.org/10.36987/jpbn.v9i2.4291","url":null,"abstract":"Metals are harmful pollutants because they cannot be broken down by living organisms. An excess of metal can become toxic. The metal in question is copper (Cu). This research aimed to determine the phytoremediation effectiveness of Acorus calamus, Pistia stratiotes, and Azolla pinnata as candidates for Cu metal absorption. This investigation employed a wholly random factorial design with two factors: plant absorbent materials comprised of three plant species (Acorus calamus, Pistia stratiotes, and Azolla pinnata) and absorption concentrations of 2 and 5 ppm. The ANOVA test was used to analyze the data, followed by the average difference test. According to the results of this study, three plants—Acorus calamus, Pistia stratiotes, and Azolla pinnata—are capable of reducing Cu metal in water. The Acorus calamus plant can reduce Cu concentrations in water from 2 ppm to 96.85% and from 5 ppm to 96.80%. Pistia stratiotes plants can reduce Cu level in water from 2 ppm to 96.50% and 5 ppm to 99.94% at concentrations of 2 and 5 ppm, respectively. The Azolla pinnata plant can reduce Cu level in water from 2 ppm to 98.50% and from 5 ppm to 96.54% at concentrations of 2 ppm and 5 ppm, respectively. The highest BCF value at a concentration of 2 ppm is in the roots of Azolla pinnata plants, at 6.77 mg/kg, followed by the leaves, at 8.88 mg/kg. The maximum BCF value at a concentration of 5 ppm for Pistia stratiotes plants is 2.26 mg/kg for the roots and 2.46 mg/kg for the foliage. The greatest concentration of TF at 2 ppm in Azolla pinnata is 1.31 mg/kg, while the maximum concentration at 5 ppm in Acorus calamus is 1.98 mg/kg","PeriodicalId":422054,"journal":{"name":"JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124207900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}