JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS最新文献_第3页

Rattan Species Inventory in Rawa Singkil Region of Conservation Forest Management Unit Area 保护林管理单位区域拉瓦辛基尔地区的藤类物种调查

JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Pub Date : 2023-11-07 DOI: 10.36987/jpbn.v9i3.4357

Nasrianti Syam, Mawaddah Putri Arisma Siregar, Eva Flourentina Kusumawardani, Laila Apriani Hasanah Harahap, Mardi Fadilah, Firman Firdauz Saputra, Perry Boy Chandra Siahaan

{"title":"Rattan Species Inventory in Rawa Singkil Region of Conservation Forest Management Unit Area","authors":"Nasrianti Syam, Mawaddah Putri Arisma Siregar, Eva Flourentina Kusumawardani, Laila Apriani Hasanah Harahap, Mardi Fadilah, Firman Firdauz Saputra, Perry Boy Chandra Siahaan","doi":"10.36987/jpbn.v9i3.4357","DOIUrl":"https://doi.org/10.36987/jpbn.v9i3.4357","url":null,"abstract":"Rawa Singkil region of Conservation Forest Management Area (KPHK) is a sanctuary for fauna teeming with an abundance of plant species. The species diversity is predominantly constituted of rattan and other non-timber forest products. This investigation seeks to compile a list of the various species of rattan found in the Rawa Singkil KPHK region. Therefore, thisÂ study was carried out in the Rawa Singkil region of KPHK were located in the Aceh Singkil Regency of Aceh Province, Indonesia. Spanning an area of 82,374 ha, this territory comprises nearly fifty percent peat ecosystem. A survey is utilized as the research strategy, with data acquisition conducted via sampling techniques. Moreover, the acquired data were subjected to descriptive analysis on the basis of morphological attributes, encompassing the following morphological characteristics: 1) the stem (including characteristics such as height, diameter, color, internode length, and thorn shape); 2) the leaf, including its length, ocrea, leaf count, leaf color, and leaf surface; 3) knee (color and outline of the knee); 4) thorn; (spine color, length of longest spines, position of spines, and length of shortest spines). Eight species of rattan were discovered in this region, i.e Plectocomia elongata Mart., Daemonorops mattanensis Becc, Daemonorops melanochaetes, Korthalsia echinometra Becc, and Calamus ornatus Blume","PeriodicalId":422054,"journal":{"name":"JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139283075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inventory of Pathogenic Fungi by Red Chili Seeds (Capsicum annuum L.) from Deli Serdang Regency, North Sumatera 北苏门答腊 Deli Serdang 地区红辣椒种子（Capsicum annuum L.）的病原真菌清单

JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Pub Date : 2023-11-07 DOI: 10.36987/jpbn.v9i3.4894

D. N. Sukapiring, Lily Novianty, Siti Nurlani Harahap

{"title":"Inventory of Pathogenic Fungi by Red Chili Seeds (Capsicum annuum L.) from Deli Serdang Regency, North Sumatera","authors":"D. N. Sukapiring, Lily Novianty, Siti Nurlani Harahap","doi":"10.36987/jpbn.v9i3.4894","DOIUrl":"https://doi.org/10.36987/jpbn.v9i3.4894","url":null,"abstract":"Red chilies known as Capsicum annuum L., hold significant importance as horticulture crops in Indonesia. The production of red chilies begins with the planting of seeds, making seed health a crucial factor in the process. Seeds contaminated with pathogens can serve as a reservoir for the transmission of diseases in the field, leading to the demise of seedlings and hindering their growth into fully mature plants, ultimately resulting in crop failure. This study used the blotter test technique to ascertain the morphology of a seed-borne pathogenic fungus. The research findings indicated that the blotter test technique detected four isolates of seed-borne pathogenic fungus, specifically TMIA with an infection rate of 0.5%, TMIB with an infection rate of 0.5%, TMIC with an infection rate of 2.5%, and TMIE with an infection rate of 3%. A single isolate of a seed-borne pathogenic fungus, named SRIA, was obtained from Sidodadi Ramunia village. The infection rate of this fungus was determined to be 0.5%. The chili seeds recovered were found to carry pathogenic fungal isolates belonging to Aspergillus sp., Penicillium sp., Verticillium sp., and Curvularia sp. based on morphological identification","PeriodicalId":422054,"journal":{"name":"JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139287714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Nutritional Content Found in Rhizophora Mangrove Fruits in Sondaken Village, Tatapaan Subdistrict, South Minahasa 南米纳哈萨省 Tatapaan 分区 Sondaken 村红树果实中的营养成分

JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Pub Date : 2023-11-07 DOI: 10.36987/jpbn.v9i3.4468

Priskila Indah Theresya Mongdong, Y. Mokosuli, N. Ogi

{"title":"The Nutritional Content Found in Rhizophora Mangrove Fruits in Sondaken Village, Tatapaan Subdistrict, South Minahasa","authors":"Priskila Indah Theresya Mongdong, Y. Mokosuli, N. Ogi","doi":"10.36987/jpbn.v9i3.4468","DOIUrl":"https://doi.org/10.36987/jpbn.v9i3.4468","url":null,"abstract":"Mangroves are fertile plants that dominate the coastal areas. The fruit of the Rhizophora mangrove can be used as food and beverage ingredients, containing numerous nutrients for the human body. The mangrove fruit contains carbohydrates, water, protein, fat, as well as secondary metabolites such as flavonoids, triterpenoids, tannins, and saponins. The aim of this research is to determine the nutritional content of the Rhizophora apiculata, Rhizophora mucronata, and Rhizophora stylosa mangrove fruits in Sondaken Village, Tatapaan Subdistrict, South Minahasa. This study employs a descriptive research method, where laboratory experiments were conducted using the SNI 01-2891-1992 method at the Baristand Manado laboratory. The nutritional analysis results for Rhizophora apiculata showed a water content of 62.17%, ash content of 1.09%, protein content of 0.59%, fat content of 0.79%, crude fiber content of 5.45%, and carbohydrate content of 35.36%. For Rhizophora mucronata, the analysis revealed a water content of 65.54%, ash content of 3.07%, protein content of 1.27%, fat content of 0.74%, crude fiber content of 7.52%, and carbohydrate content of 29.38%. Lastly, Rhizophora stylosa had a water content of 64.77%, ash content of 3.547%, protein content of 1.30%, fat content of 1.63%, crude fiber content of 7.14%, and carbohydrate content of 28.76%. These results indicate that mangrove fruits have numerous benefits and contain valuable nutrients and good nutritional value for the body. By preserving the mangrove ecosystem and utilizing the nutrients found in the fruits and leaves, there is an opportunity to explore their potential as alternative natural remedies","PeriodicalId":422054,"journal":{"name":"JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS","volume":"72 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139285938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Duration Effects of Shrimp Paste Storage on ALT of Mold Colonies: Variations by Brand and Pre-Standard Treatment 虾酱储存时间对霉菌菌落 ALT 的影响：不同品牌和标准前处理的差异

JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Pub Date : 2023-11-01 DOI: 10.36987/jpbn.v9i3.4690

Nur Laila Syafitri, U. S. Hastuti, S. Prabaningtyas

{"title":"Duration Effects of Shrimp Paste Storage on ALT of Mold Colonies: Variations by Brand and Pre-Standard Treatment","authors":"Nur Laila Syafitri, U. S. Hastuti, S. Prabaningtyas","doi":"10.36987/jpbn.v9i3.4690","DOIUrl":"https://doi.org/10.36987/jpbn.v9i3.4690","url":null,"abstract":"Shrimp paste is a commonly employed condiment or flavoring in culinary preparations. As a result of the nutrients present in shrimp paste, fungi and other microbial contaminants are able to proliferate and develop on the paste. This study aims as follows: 1) to determineÂ ALT measurements of mold colonies to assess the quality of two brands of pre-treated shrimp paste; and 2) to identifyÂ contaminating mold species present in steamed and unsteamed shrimp paste prior to storage. Descriptive quantitative and qualitative research methods wereÂ employed within the Biology Department of FMIPA UM's Microbiology Laboratory. A series of three samplings were conducted on treated shrimp paste brands A and B, one week apart between each sampling. A volume of 90 ml of 0.1% peptone water was used to dissolve 10 grams of shrimp paste, resulting in a dilution of 10-1; this process was repeated until the dilution reached 10-5. 0. The surface of PDA (Potato Dextrose Agar) medium was inoculated with 1 ml of suspension from each dilution. The medium was subsequently incubated at a temperature of 25oC for 7x24 hours. The findings of this study indicate the following: 1) At the 28th day of storage, both brands of shrimp paste that underwent treatment with steamed and unsteamed shrimp paste remained of acceptable quality for human consumption. 2) In steamed and unsteamed shrimp paste, thirteen species of mold contaminants were identified: Chrysosporium corda, Cladosporium sphaerospermum, Penicillium frequentans, Penicillium chrysogenum, Rhizoctonia sp1., Aspergillus candidus, Fusarium equeseti, Colletotrichum ti, Moniliella acetobutens, and Rhizoctonia sp3","PeriodicalId":422054,"journal":{"name":"JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139296269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening For Lipolytic Bacteria from Bonoloyo Cemetery, Surakarta 苏腊卡尔塔博诺洛约墓地脂肪分解细菌筛选

JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Pub Date : 2023-11-01 DOI: 10.36987/jpbn.v9i3.4944

Heni Sulistyo Rini, Erma Musbita Tyastuti, Yasir Siddiq, Triastuti Rahayu

{"title":"Screening For Lipolytic Bacteria from Bonoloyo Cemetery, Surakarta","authors":"Heni Sulistyo Rini, Erma Musbita Tyastuti, Yasir Siddiq, Triastuti Rahayu","doi":"10.36987/jpbn.v9i3.4944","DOIUrl":"https://doi.org/10.36987/jpbn.v9i3.4944","url":null,"abstract":"A cemetery is a burial place managed by the government. Inside the TPU, the body is decomposed by microorganisms, one of which is lipolytic bacteria, because the human body is composed of about 12.5â€“13.60% lipids. Research on lipolytic bacteria from TPU in Indonesia has not been found, even though it has the potential to obtain lipolytic bodies. Therefore, this research was conducted to select lipolytic bacteria from TPU and provide simple assistance. A total of 45 bacterial isolates from TPU Bonoloyo Surakarta were selected for their lipolytic activity using tributyrin agar media. Lipolytic activity was determined using the lipolytic index (LI). Bacterial isolates with lipolytic potential were identified based on colony morphology and Gram staining. The results showed that 30 isolates (67%) showed lipolytic activity, with the highest LI value of 5.43 (BLB 9) after 2x24 hours of incubation. Bacterial isolates that have lipolytic activity are white colonies with circular shapes. The results of Gram staining showed that the bacterial isolates belonged to a group of Gram-negative bacteria in the form of cocci. The conclusion put forward is that public cemeteries (TPU) have the potential to store isolates of lipolytic bacteria dominated by Gram-positive bacteria by as much as 57%","PeriodicalId":422054,"journal":{"name":"JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS","volume":"60 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139295292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Secondary Metabolites of Trichoderma sp. as Antifungal Against Rice Seed-borne Pathogen Fungi 毛霉的次级代谢产物对水稻种子病原真菌的抗真菌作用

JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Pub Date : 2023-11-01 DOI: 10.36987/jpbn.v9i3.4822

Anisa Rizki Amalia, A. Purnawati, Endang Triwahyu Prasetyawati, Vika Yanti

{"title":"Secondary Metabolites of Trichoderma sp. as Antifungal Against Rice Seed-borne Pathogen Fungi","authors":"Anisa Rizki Amalia, A. Purnawati, Endang Triwahyu Prasetyawati, Vika Yanti","doi":"10.36987/jpbn.v9i3.4822","DOIUrl":"https://doi.org/10.36987/jpbn.v9i3.4822","url":null,"abstract":"The use of quality seeds is necessary to increase rice productivity. The existence of pathogenic fungi infections causes the quality of rice seeds to decrease and impacts the ability to grow plants. Trichoderma sp. is known to produce secondary metabolites that can inhibit the growth of pathogens. This study aimed to examine the ability of the secondary metabolites of Trichoderma sp. at various concentration levels and immersion time to suppress pathogenic fungi infections carrying rice seeds. The research was carried out by giving seed immersion treatment. Then identify the pathogenic fungi the seeds carry for further testing with the blotter test method. The parameters tested were infection rate, infection suppression, and seed viability. The identification results showed types of pathogenic fungi carried by rice seeds included Fusarium sp., Alternaria sp., Aspergillus sp. Penicillium sp., and Rhizopus sp. Research also shows that the secondary metabolites of Trichoderma sp. are effective in improving the quality of rice seeds. The test results showed that the secondary metabolites of Trichoderma sp. can suppress the level of pathogenic fungi infection. There was no interaction between the treatment of metabolite concentrations and immersion time on all test parameters. Seed immersion treatment with secondary metabolites in the 5-20% concentration range has effectively reduced fungi infection rates and increased seed viability. Meanwhile, various levels of soaking time did not affect reducing the level of fungi infection but instead affected the viability of the rice seeds.","PeriodicalId":422054,"journal":{"name":"JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS","volume":"266 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139297670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Diversity of Reptile Species in Mangrove Ecotourism of Kampung Rawa Mekar Jaya, Siak Regency 甘榜拉瓦麦卡加亚红树林生态旅游中爬行动物物种多样性

JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Pub Date : 2023-07-31 DOI: 10.36987/jpbn.v9i2.4266

Aldi Ardiansyah Putra, Defri Yoza, S. C. Setyawatiningsih

{"title":"The Diversity of Reptile Species in Mangrove Ecotourism of Kampung Rawa Mekar Jaya, Siak Regency","authors":"Aldi Ardiansyah Putra, Defri Yoza, S. C. Setyawatiningsih","doi":"10.36987/jpbn.v9i2.4266","DOIUrl":"https://doi.org/10.36987/jpbn.v9i2.4266","url":null,"abstract":"Rawa Mekar Jaya mangrove ecotourism is the last mangrove forest on the east coast of Sumatra Island. This mangrove forest is a home for reptiles. The reptilian fauna of the Mekar Jaya mangrove has the potential to become an ecotourism object. This study aims to determine the diversity, evenness, abundance, and status of reptile species in the Mekar Jaya mangrove ecotourism. Four 3 x 400 m transects were conducted in two habitat types: aquatic (10 m of bank) and terrestrial (30 m of bank). Observations were made in the morning and evening at low tide and repeated five times at three-day intervals. Direct collection of reptile data using a visual survey (VES) and passive sampling (glue trap). The researchers managed to capture 52 individuals, which were reptiles, consisting of 4 species (Draco sumatranus, Hemidactylus platyurus, Mabuya multifasciata, and Varanus savator) found directly in the field. The results of the interviews with the managers indicate that there are 2 species of crocodiles: Crocodylus porosus and Tomistoma schlegelii. Shannon-Wiener diversity index value was medium (H'=1.02). Evenness index was labile (0.57). The highest species abundance was M. multifasciata (0.65), while the lowest abundance was H. platyurus (0.12). All reptile species found were not protected. V. salvator is classified as a low-risk species (LC/Minor Concern) and is listed in Appendix II of CITES. Two crocodile species reported by the community are protected. T. schlegelii is vulnerable and listed in Appendix I of CITES. C. porosus is low-risk and listed in CITES Appendix II.","PeriodicalId":422054,"journal":{"name":"JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122056704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Formulation of Red Beet (Beta vulgaris. L) and Aloe Vera (Aloe vera) Gel Extracts as Anti-Aging 红甜菜的配方研究。L)和芦荟(芦荟)凝胶提取物抗衰老

JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Pub Date : 2023-07-31 DOI: 10.36987/jpbn.v9i2.4478

Siti Maimunah, Andre Prayoga

{"title":"Formulation of Red Beet (Beta vulgaris. L) and Aloe Vera (Aloe vera) Gel Extracts as Anti-Aging","authors":"Siti Maimunah, Andre Prayoga","doi":"10.36987/jpbn.v9i2.4478","DOIUrl":"https://doi.org/10.36987/jpbn.v9i2.4478","url":null,"abstract":"Anti-Aging can prevent or slow down the effects of skin aging. This study aims to formulate extracts of beetroot (Beta vulgaris. L) and Aloe Vera (Aloe Vera) in gel preparations and test their anti-aging effectiveness. This research was conducted at the Laboratory of Pharmaceutical Chemistry, University of Sari Mutiara Indonesia. The tools used are skin analyzer and moisture checker. B. vulgaris Samples from Brastagi, Karo Regency and A.vera taken from Rantauprapat, Labuhan Batu Regency. Methods of implementation Beet extraction was extracted with 96% ethanol solvent, and A.vera extract from the juicer. The irritation test on 12 volunteers used nettle extract cream with the highest concentration of 0.5%. Anti-aging activity testing used 12 female volunteers aged 19-22 years, who were divided into 4 groups, namely 3 volunteers (A; Blank), administration of beetroot extract gel (B. vulgaris. L) and A.vera (B) : 3%), (C: 6%), and (D: 9%). 3. Parameters measured include moisture content, number of pores and wrinkles. Data distribution was analyzed using the One sample Kolmonogrov-Smirnov Test method. Then proceed with the analysis of the One-Way Anova method. The results of research on beet (B. vulgaris. L) and Aloe Vera (A.vera) gel preparations can be formulated in a gel dosage form. pH 6.9, W/O emulsion type, viscosity 2500-3500 cps, non-irritating, and stable on storage for 28 days. Beet fruit extract (B. vulgaris) and aloe vera viscous extract (A.vera) can be formulated in the form of an anti-aging gel that can provide an anti-aging effect at the best concentration, namely at a concentration of 9% by increasing skin moisture to 40.24%; smaller pores 0.53%; and reduce wrinkles by 0.33%","PeriodicalId":422054,"journal":{"name":"JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122278792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioactive Contents, BSLT Toxicity and antioxidants from Apis dorsata Binghami and Apis mellifera nest extracts 白桦和蜜蜂巢提取物的生物活性含量、BSLT毒性及抗氧化剂研究

JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Pub Date : 2023-07-29 DOI: 10.36987/jpbn.v9i2.4514

Reinny Silvana Tuegeh, H. Sumampouw, M. N. Tanor, Y. Mokosuli

{"title":"Bioactive Contents, BSLT Toxicity and antioxidants from Apis dorsata Binghami and Apis mellifera nest extracts","authors":"Reinny Silvana Tuegeh, H. Sumampouw, M. N. Tanor, Y. Mokosuli","doi":"10.36987/jpbn.v9i2.4514","DOIUrl":"https://doi.org/10.36987/jpbn.v9i2.4514","url":null,"abstract":"Beenest is rich in secondary metabolites because honeycombs, among others, are formed from plant resins (propolis). This study aimed to analyze differences in flavonoid content and in vitro antioxidant activity of Apis dorsata Binghami and Apis mellifera nest extracts. The samples used were A. dorsata from North Sulawesi and A. mellifera from South Sulawesi. The honeycomb was extracted using 95% ethanol solvent. Honeycomb extract was analyzed for its flavonoid content by the HPLC method, toxicity was tested by the BSLT method, and in vitro antioxidant activity was tested by the DPPH method. The results of the analysis of flavonoids showed that A. dorsata nest extract produced 21 types of compounds while A. mellifera produced 26 types of compounds. The toxicity test results showed that the A. dorsata nest extract had a better LC50 of 245,691 mg/l than the A. mellifera nest extract with an LC50 of 443,701 mg/l. The in vitro antioxidant test results of A. dorsata nest extract were more robust, namely IC 50 1.161 mg/l, compared to A. mellifera nest extract IC 50 2.404 mg/l. However, both were included in the category of powerful antioxidants. In vitro, anticancer test results on MCF-7 cells, A. mellifera nest extract was active with IC 50 100.02mg/l. Compared to A. dorsata extract, it was active with IC 50 102.217mg/l, but the two were not significantly different. Based on the analysis of flavonoid content, toxicity test, and antioxidant test, A.dorsata and A.mellifera honeycomb extracts have potential as in vitro antioxidants","PeriodicalId":422054,"journal":{"name":"JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS","volume":"7 4","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121015853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In Vitro Antidiabetic Activity and Bioactive Ingredients of Apis mellifera and Apis dorsata Binghami Nest Extracts 蜜蜂和杜鹃冰哈密巢提取物体外抗糖尿病活性及生物活性成分研究

JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Pub Date : 2023-07-29 DOI: 10.36987/jpbn.v9i2.4511

Henike Sibala, O. Naharia, N. Manampiring, Y. Mokosuli

{"title":"In Vitro Antidiabetic Activity and Bioactive Ingredients of Apis mellifera and Apis dorsata Binghami Nest Extracts","authors":"Henike Sibala, O. Naharia, N. Manampiring, Y. Mokosuli","doi":"10.36987/jpbn.v9i2.4511","DOIUrl":"https://doi.org/10.36987/jpbn.v9i2.4511","url":null,"abstract":"Indonesia ranks 7th out of ten countries with diabetes mellitus, which continues to increase yearly. Honeycomb is rich in secondary metabolites formed from plant resins (propolis) which contain alkaloids, flavonoids, saponins, tannins, steroids and triterpenes. The research aimed to analyze the comparison of flavonoid content and antidiabetic activity with the enzyme α-glucosidase In Vitro nest extract of A. mellifera and A. dorsata Binghami. The samples used were nests of A. mellifera from northern Toraja, southern Sulawesi and A. dorsata Binghami from Southeast Minahasa, North Sulawesi. Using 96% ethanol, HPLC method of flavonoid testing and in vitro antidiabetic activity testing using α-glucosidase enzymes. The flavonoid content results were based on the number of peaks produced and the retention time of A. mellifera produced 26 compounds, and A. dorsata Bingham produced 21 compounds. The results of in vitro antidiabetic tests with α-glucoside enzyme inhibitors obtained IC50 values from the nest extract of A. mellifera of 3.605 mg/L and the nest extract of A. dorsata Binghami of 4.992 mg/L. The extract from A. mellifera had better antidiabetic potential than that of A. dorsata Binghami, although the difference is insignificant.","PeriodicalId":422054,"journal":{"name":"JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS","volume":"266 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133726071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0