DOKLADY NATSIONALNOI AKADEMII NAUK BELARUSI最新文献_第2页

Determining the methylation status of the promoter regions of MARCH11, HOXA9, PTGDR, and UNCX genes in patients with non-small cell lung cancer 测定非小细胞肺癌患者中MARCH11、HOXA9、PTGDR和UNCX基因启动子区域的甲基化状态

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DOKLADY NATSIONALNOI AKADEMII NAUK BELARUSI Pub Date : 2023-09-01 DOI: 10.29235/1561-8323-2023-67-4-300-306

Е. П. Михаленко, А. Н. Щаюк, Т. В. Никитинская, Ю. В. Полюхович, С. В. Кубрак, М. Н. Шепетько, А. В. Кильчевский, Alena P. Mikhalenka, A. Shchayuk, Tatsiana V. Nikitinskaya, Yu. V. Paliukhovich, Sviatlana V. Kubrak, Michail N. Shapetska, Academician Aleksandr, V. Kilchevsky

{"title":"Determining the methylation status of the promoter regions of MARCH11, HOXA9, PTGDR, and UNCX genes in patients with non-small cell lung cancer","authors":"Е. П. Михаленко, А. Н. Щаюк, Т. В. Никитинская, Ю. В. Полюхович, С. В. Кубрак, М. Н. Шепетько, А. В. Кильчевский, Alena P. Mikhalenka, A. Shchayuk, Tatsiana V. Nikitinskaya, Yu. V. Paliukhovich, Sviatlana V. Kubrak, Michail N. Shapetska, Academician Aleksandr, V. Kilchevsky","doi":"10.29235/1561-8323-2023-67-4-300-306","DOIUrl":"https://doi.org/10.29235/1561-8323-2023-67-4-300-306","url":null,"abstract":"The aim of this study was to determine the methylation status of the promoter regions of MARCH11, HOXA9, PTGDR, and UNCX genes in the tumor and non-tumor lung tissue in patients with non-small cell lung cancer (NSCLC). A relative level of methylation of the promoter regions of MARCH11, HOXA9, PTGDR, and UNCX genes was determined by the quantitative methylation-specific PCR in 73 patients with NSCLC. The quantitative methylation-specific reaction was performed both for tumor tissue samples and non-tumor tissue samples of the same patient. For each of the samples, a reaction was set both by the investigated genes (MARCH11, UNCX, HOXA9, and PTGDR) and by the reference beta-actin gene (β-actin). Positive levels of methylation of the HOXA9 gene were established for 83.5 % patients; the MARCH11 gene – for 80.8 % patients; the PTGDR gene – for 68.4 % patients; the UNCX gene – for 84.9 % patients. In the study group of patients with NSCLC, significant differences were found in the relative levels of methylation of the promoter regions of MARCH11, HOXA9, PTGDR, and UNCX genes in the tumor and non-tumor lung tissue. The data suggest that hypermethylation of MARCH11, HOXA9, PTGDR, and UNCX genes may play a role in NSCLC tumor progression.","PeriodicalId":41825,"journal":{"name":"DOKLADY NATSIONALNOI AKADEMII NAUK BELARUSI","volume":null,"pages":null},"PeriodicalIF":0.1,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43776346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Analysis of transformation of a thin graphite-like carbon layer on the detonation nanodiamond surface into diamond 爆轰纳米金刚石表面薄石墨样碳层转化为金刚石的分析

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DOKLADY NATSIONALNOI AKADEMII NAUK BELARUSI Pub Date : 2023-09-01 DOI: 10.29235/1561-8323-2023-67-4-331-339

P. Vityaz, V. Senyut, A. M. Parnitskiy

{"title":"Analysis of transformation of a thin graphite-like carbon layer on the detonation nanodiamond surface into diamond","authors":"P. Vityaz, V. Senyut, A. M. Parnitskiy","doi":"10.29235/1561-8323-2023-67-4-331-339","DOIUrl":"https://doi.org/10.29235/1561-8323-2023-67-4-331-339","url":null,"abstract":"The article considers the process of obtaining nanostructured diamond materials due to phase transformation of thin films based on carbon non-diamond forms on the nanodiamond surface into diamond. For the synthesis of nanostructured diamond polycrystalline superhard material, various variants of the initial mixture based on nanodiamond with non-diamond carbon on the surface are proposed (surface-graphitized nanodiamond, surface-graphitized nanodiamond with addition of purified nanodiamond, detonation diamond-containing charge with a surface layer of “amorphous” carbon, including purified nanodiamond additives). The influence of the structure of a thin non-diamond layer on the parameters of graphite to diamond transition is revealed. For a carbon film with a disordered structure (the so-called “amorphous” carbon), the transition pressure to diamond will be about 10–15 GPa, which is significantly higher than the phase transformation pressure for thin graphite films in this temperature range. It is shown that the increase in the pressure of transformation of a thin layer of “amorphous” carbon is caused by its lower surface energy compared to the surface energy of graphite. It has been established that the region of transformation of a thin graphite-like film with a thickness of about 1 nm, formed on the 2–10 nm nanodiamond surface into diamond will be below the graphite-diamond equilibrium line in the temperature range of 1000– 2500 °C. Additional introduction of purified nanodiamond particles leads to a decrease in the pressure of transformation of thin layers of non-diamond carbon into a diamond structure from 10–15 to 2–7 GPa, which is due to the effect of the surface of catalytically active diamond nanoparticles on the thermodynamic stimulus of phase transformation.","PeriodicalId":41825,"journal":{"name":"DOKLADY NATSIONALNOI AKADEMII NAUK BELARUSI","volume":null,"pages":null},"PeriodicalIF":0.1,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44316488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of the stress-strain state of problematic sections of the shaft of the mine using computer simulation 利用计算机模拟分析了矿井井筒问题断面的应力-应变状态

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DOKLADY NATSIONALNOI AKADEMII NAUK BELARUSI Pub Date : 2023-09-01 DOI: 10.29235/1561-8323-2023-67-4-322-330

Denis A. Diulin, Academician Viktor, Y. Prushak, Maryna G. Gegedesh, Д. А. Диулин, В. Я. Прушак, М. Г. Гегедеш

{"title":"Analysis of the stress-strain state of problematic sections of the shaft of the mine using computer simulation","authors":"Denis A. Diulin, Academician Viktor, Y. Prushak, Maryna G. Gegedesh, Д. А. Диулин, В. Я. Прушак, М. Г. Гегедеш","doi":"10.29235/1561-8323-2023-67-4-322-330","DOIUrl":"https://doi.org/10.29235/1561-8323-2023-67-4-322-330","url":null,"abstract":"The article presents the computer simulation results for the stress-strain state of the lining sections of the shaft No. 1 of mine 3 of JSC Belaruskali that were obtained using visual and instrumental investigation of the mine lining state. The loading parameters of the shaft take into account the specific feature of the lateral pressure calculation in the considered location. This means that the shaft intersects vertically rocks different in their physical and mechanical properties. Computer simulation of loading of the most problematic vertical mine shaft sections observed by the lining examination results was performed using the Static Structural component of the ANSYS Workbench engineering software package. Distribution diagrams of longitudinal normal, shear and von Mises equivalent stresses in the vertical mine shaft lining are obtained for the design and residual values of the cast-iron tubing thickness. A comparative analysis of the mine shaft residual bearing capacity for various parameters of the lining elements was carried out. Based on the computer simulation results, the conclusions were made on the bearing capacity of the shaft No. 1 of mine 3 of JSC Belaruskali and the recommendations for a further safe operation of the mine shaft were suggested.","PeriodicalId":41825,"journal":{"name":"DOKLADY NATSIONALNOI AKADEMII NAUK BELARUSI","volume":null,"pages":null},"PeriodicalIF":0.1,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48910014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anti-coronavirus properties of brassinosteroids 油菜素甾体的抗冠状病毒特性

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DOKLADY NATSIONALNOI AKADEMII NAUK BELARUSI Pub Date : 2023-09-01 DOI: 10.29235/1561-8323-2023-67-4-295-299

V. Zhabinskii, Alexey M. Matorin, O. Savinova, E. Boreko, Academician Vladimir A. Khripach, Член-корреспондент В. Н. Жабинский, А. М. Маторин, О.В. Савинова, Е.И. Бореко, В. А. Хрипач

引用次数: 0

Sperm double-strand DNA breaks in patients with normozoospermia and pathozoospermia. 正常精子症和病态精子症患者精子双链DNA断裂。

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DOKLADY NATSIONALNOI AKADEMII NAUK BELARUSI Pub Date : 2023-09-01 DOI: 10.29235/1561-8323-2023-67-4-307-314

И. Д. Кужель, О. В. Прибушеня, И. В. Наумчик, И. В. Курлович, Н. И. Рябоконь, Irena D. Kuzhal, Oksana V. Pribushenya, Irina V. Naumchik, Ivan V. Kurlovich, Nadezhda I. Ryabokon

{"title":"Sperm double-strand DNA breaks in patients with normozoospermia and pathozoospermia.","authors":"И. Д. Кужель, О. В. Прибушеня, И. В. Наумчик, И. В. Курлович, Н. И. Рябоконь, Irena D. Kuzhal, Oksana V. Pribushenya, Irina V. Naumchik, Ivan V. Kurlovich, Nadezhda I. Ryabokon","doi":"10.29235/1561-8323-2023-67-4-307-314","DOIUrl":"https://doi.org/10.29235/1561-8323-2023-67-4-307-314","url":null,"abstract":"The levels of double-strand DNA breaks as a severe disruption of genome integrity were studied using the neutral version of the comet assay in the sperm samples of the men of the Belarusian population with normozoospermia and pathozoospermia, including asthenozoospermia, oligozoospermia, oligoasthenozoospermia, and other combined forms of pathozoospermia. It was demonstrated that double-strand DNA breaks have a large proportion (about 44–50 % on average) of the total number of DNA damage (singleand double-strand breaks, alkaline-labile sites, etc.) analyzed with the alkaline version of the comet assay recommended by the World Health Organization. Higher levels of sperm double-strand DNA breaks were established in the pathozoospermia group and in asthenozoospermia subgroup compared to the normozoospermia group. It was also shown that the levels of double-strand breaks observed at pathozoospermia correspond to the mutagenic effect in vitro of high concentrations (10–30 μg/ml) of bleomycin sulfate that acts as a strong radiomimetic, which points to a significant disruption of the DNA integrity at pathozoospermia. In general, the data obtained demonstrate the usefulness of the sperm double-strand DNA break analysis for male infertility diagnostics.","PeriodicalId":41825,"journal":{"name":"DOKLADY NATSIONALNOI AKADEMII NAUK BELARUSI","volume":null,"pages":null},"PeriodicalIF":0.1,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48834824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Crystal and magnetic structures of solid solutions based on BiFeO3 BiFeO3固溶体的晶体和磁性结构

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DOKLADY NATSIONALNOI AKADEMII NAUK BELARUSI Pub Date : 2023-09-01 DOI: 10.29235/1561-8323-2023-67-4-279-286

D. Karpinsky, S. I. Latushkа, D. V. Zheludkevich, A. N. Chobot, V. Kruplevich, V. K. Dolgiy, T. V. Latushkа, K. N. Neklyudov, M. Silibin, E. V. Budemko

引用次数: 0

Metric theory of diophantine approximation and asymptotic estimates for the number of polynomials with given discriminants divisible by a large power of a prime number 丢芬图近似的度量理论及可被一个素数的幂次整除的具有给定判别式的多项式数目的渐近估计

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DOKLADY NATSIONALNOI AKADEMII NAUK BELARUSI Pub Date : 2023-09-01 DOI: 10.29235/1561-8323-2023-67-4-271-278

V. Bernik, D. V. Vasilyev, N. I. Kalosha, Zh. I. Panteleeva

引用次数: 0

Elastification of the arterial wall by high-intensity low-frequency ultrasound 高强度低频超声对动脉壁的弹性作用

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DOKLADY NATSIONALNOI AKADEMII NAUK BELARUSI Pub Date : 2023-09-01 DOI: 10.29235/1561-8323-2023-67-4-287-294

I. Adzerikho, A. I. Kulak, T. Vladimirskaya, T. V. Leonchik, S. N. Chur, V. T. Minchenya, S. V. Shil’ko

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Strength and crack resistance of cement composites under multilevel reinforcement 多级配筋条件下水泥复合材料的强度和抗裂性能

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DOKLADY NATSIONALNOI AKADEMII NAUK BELARUSI Pub Date : 2023-09-01 DOI: 10.29235/1561-8323-2023-67-4-340-344

S. A. Zhdanok, S. Leonovich, E. Sadovskaya, E. Polonina

引用次数: 0

Image analysis of brain cortex cells in vitro using deep learning method 基于深度学习方法的体外脑皮层细胞图像分析

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DOKLADY NATSIONALNOI AKADEMII NAUK BELARUSI Pub Date : 2023-09-01 DOI: 10.29235/1561-8323-2023-67-4-315-321

A. A. Denisov, A. V. Nikiforov, A. V. Bahdanava, S. Pashkevich, N. S. Serdyuchenko

{"title":"Image analysis of brain cortex cells in vitro using deep learning method","authors":"A. A. Denisov, A. V. Nikiforov, A. V. Bahdanava, S. Pashkevich, N. S. Serdyuchenko","doi":"10.29235/1561-8323-2023-67-4-315-321","DOIUrl":"https://doi.org/10.29235/1561-8323-2023-67-4-315-321","url":null,"abstract":"The article presents a method for analyzing images of cultured cortical cells for a quantitative analysis of the parameters of development of biological neural networks using machine learning approaches. We have developed software modules for segmentation of images into cells, clusters, and neurites using the neural network model and the deep learning method; a training set of images of cultivated neurons and corresponding segmentation masks have been generated. The results were validated by analyzing the development of cultivated neurons in vitro based on the length count of neutrites at different growth stages of the culture. The developed methods for monitoring the processes of formation of biological neuronal networks based on the analysis of the neuronal growth under different conditions and on different substrates provide an opportunity to monitor the processes of stem cell differentiation in the neurogenic direction. The results can be used in monitoring the formation of organoids in bioengineering applications, as well as in modeling the processes of nerve tissue regeneration.","PeriodicalId":41825,"journal":{"name":"DOKLADY NATSIONALNOI AKADEMII NAUK BELARUSI","volume":null,"pages":null},"PeriodicalIF":0.1,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44719905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0