Asian Journal of Biological and Life sciences最新文献

{"title":"Histological findings of Lasia spinosa extract in Arthritic Model","authors":"Mrityunjay Kumar, B. Kakoti, Sudarshana Borah, Kabita Mahato, K. Barman, Bhanita Das, A. Bora, P. Kalita, Innocent Sutnga","doi":"10.5530/ajbls.2022.11.70","DOIUrl":"https://doi.org/10.5530/ajbls.2022.11.70","url":null,"abstract":"Background: Arthritis is a phrase that encompasses more than 100 different medical disorders. Arthritis is a condition in which one or more joints become inflamed. Arthritis causes cartilage to break down. A joint’s cartilage protects it and allows it to move freely. When pressure is applied to the joint, such as when walking, cartilage absorbs the stress. The bones rub together when there isn’t enough cartilage, producing discomfort, swelling (inflammation), and stiffness. Lymphadenopathy, Oedema, ocular inflammation, glaucoma Bursitis, urethritis, tenosynovitis (tendon sheath effusions) (swollen bursa), Diarrhea, Ulcers in the orogenital area. Materials and Methods: Lasia spinosa is a herbal medication with roots in many different traditional medical systems all over the world. Results: In this investigation, histopathology of the synovial joint revealed that Lasia spinosa group therapy reduced vascularity, lymphocytic infiltration, there was no thickening of the synovial membrane and no lymphoid follicles, indicating angiogenesis. Conclusion: This study reveals the histology of synovial joints, treated with the Lasia spinosa that reduced the vascularity, lymphocytic infiltration, and angiogenesis without causing the synovial membrane to thicken. Our research findings indicated that Lasia spinosa has potent anti-arthritic properties.","PeriodicalId":413740,"journal":{"name":"Asian Journal of Biological and Life sciences","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114903282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of blaCTX-M, blaTEM, and blaSHV Genes of Extended-Spectrum Beta Lactamase-Producing Escherichia coli in Aquaculture in Asia: A Systematic Review","authors":"Allysah Rhein C. Tolentino, Alyanna Renae P. Opeda, Lorraine L. Naniong, Lea Janine I. Paralejas, Faith Marie M. Porciuncula, Irah Mae A. Getuiza, Czarene Bon Harvey G. Evangelista, C.P. Tolenada","doi":"10.5530/ajbls.2022.11.31","DOIUrl":"https://doi.org/10.5530/ajbls.2022.11.31","url":null,"abstract":"","PeriodicalId":413740,"journal":{"name":"Asian Journal of Biological and Life sciences","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114290648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enumeration and Identification of Surface Endophytes of Solanum lycopersicum and their Seasonal Recurrence","authors":"Lemma Abayneh, Abreham Ayele, Tesfaye Lamore, Denebo Sebaro2, V. Srinivasan","doi":"10.5530/ajbls.2022.11.71","DOIUrl":"https://doi.org/10.5530/ajbls.2022.11.71","url":null,"abstract":"Background: Endophytes have been identified in various plant species; however, only a few studies have been conducted to explore the endophytic fungi folklore in fruit-bearing plants. The present study was conducted to enumerate the foliar endophytes of Solanum lycopersicum . Materials and Methods: The leaf samples of S. lycopersicum were harvested in four different seasons during the year 2020-2021. Samples were surface sterilized with 0.2% HgCl 2 , inoculated onto potato dextrose agar, amended with 0.12mg/ml chloramphenicol and incubated at 25-32°C with exposure to light and dark cycles. Identification was done by morphological typing. Results: Leaf samples harboured endophytes in all sampling seasons; however, season Sept-Nov which observed higher rainfall, witnessed more endophytes than the other seasons. The diversity of endophyte assemblage identified were hyphomycete and coelomycete species. Geotrichum sp., Phomopsis sp., Trichoderma sp., and Humicola sp. have proven their recurrence. Colletotrichum sp., being the dominant genus with higher colonization frequency, was found to be harboured in all sampling seasons. Conclusion: The study is the first of its kind in exploring the fungal endophytic profile in S. lycopersicum . Hence further studies are warranted to explore if the inherent endophytes of this plant confer any role in the metabolic physiology of the plant or host-pathogen interaction.","PeriodicalId":413740,"journal":{"name":"Asian Journal of Biological and Life sciences","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129320552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preliminary Phytochemical Screening and GC-MS Analysis of Ethanolic Stem Extract of the Ethnomedicinal Plant- Tinospora crispa (L.) Miers [Menispermaceae]","authors":"J. Akila Shree, C. Krishnaveni","doi":"10.5530/ajbls.2022.11.65","DOIUrl":"https://doi.org/10.5530/ajbls.2022.11.65","url":null,"abstract":"Tinospora crispa (L.) Miers belongs to the family Menispermaceae and is used as a traditional medicine in China, Malaysia, Philippines, etc. It is one of the most popular local medicinal plants. It is also called Makabühai, which means “to give life”. The GCMS analysis revealed the presence of 20 compounds in the stem extract of Tinospora crispa . Some of the compounds are 1,2,3-Propanetriol, diacetate (CAS) Diacetin, 3-Tetradecene, (Z)- (CAS), 4-Penten-1-ol, trimethylsilyl ether, Diethyl Phthalate 1,2-benzene dicarboxylic acid, diethyl ester, 9-Eicosene, (E)- (CAS), Benzyl Benzoate, 9-Octadecenoic acid (Z)- (CAS) Oleic acid, Phosphonic acid, dioctadecyl ester (CAS) Di(n-octadecyl) phosphite, 9,12-Octadecadienoic acid (Z,Z)-, 9,12,15-Octadecatrienoic acid, (Z,Z,Z)-(CAS) Linolenic acid, 3-furylmethanol, 1,2-Benzenedicarboxylic acid, bis(2-ethylhexyl) ester (CAS) Bis(2-ethylhexyl) phthalate. This plant contains compounds such as alkaloids, saponins, glycosides, tannins, and flavonoids. These compounds have antipyretic, antiparasitic, antifungal, antidiabetic and antitumor properties.","PeriodicalId":413740,"journal":{"name":"Asian Journal of Biological and Life sciences","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124298364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two-dimensional Gel Analysis of Total Seed Protein of Horse Gram (Macrotyloma uniflorum) for Identification of their Proteomic Diversity","authors":"N. Sharma, S. Bisht, S. Gupta, Seeta Dewali, N. Parveen, R. Pant","doi":"10.5530/ajbls.2022.11.45","DOIUrl":"https://doi.org/10.5530/ajbls.2022.11.45","url":null,"abstract":"Horse gram ( Macrotyloma uniflorum ) is a popular pulse in the Kumaun region of Uttarakhand, locally known as Gahat dal and Kulti. An experiment was conducted with 5 horse gram cultivars having different grain colors. Total seed protein was isolated and quantified with standard protocols. Five protein samples were subjected to one-dimensional (1D) and two-dimensional (2D) gel for proteomic analysis. 300g of each sample was loaded onto immobilized pH gradient (IPG) strips (3–10 pH Linear, 18 cm) and kept for Iso-Electric Focusing (IEF) and SDS-PAGE. The gels were scanned using a scanner and analyzed for proteomic diversity. Basically, two major clusters were constructed, and samples 1, 2, and 3 are in group one and 4 and 5 are in group two, and group one has two further sub-clusters. Sample one was separately clustered based on the number of spots presented on the 2D gel and based on its color. The analysis of variance revealed that the genotypes observed had highly significant differences. Further study is needed for the identification of unique peptides presented by protein sequencing. The proteomic analysis of total seed proteins could be useful for the development of nutraceuticals and food-to-food fortification. This study’s current proteome and other proteomic information will be extremely useful to horse gram breeding programmes.","PeriodicalId":413740,"journal":{"name":"Asian Journal of Biological and Life sciences","volume":"102 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117336552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Novel Design Chewing Gum of Calcium Ions Isolated from Natural Source","authors":"V. Muruganantham, B. Venkateswarlu, Murali Anjappa Chetty","doi":"10.5530/ajbls.2022.11.47","DOIUrl":"https://doi.org/10.5530/ajbls.2022.11.47","url":null,"abstract":"A pharmaceutical excipient includes calcium carbonate. It is widely used as a diluent in solid dosage forms. As a base for pharmaceutical and dental treatments, as a buffering and dissolution aid for dispersible tablets, as a food additive, and as a calcium supplement, this is utilised. Mineral salts, chiefly calcium carbonate, make up a significant percentage of the eggshell, accounting for around 95 percent of the shell. This Layer farm produces such a large amount of egg shells that their disposal is a problem for the environment. Oral drug delivery system researchers have created new formulations and technologies. The studies show the importance of the oral route among patients. There is an abundance of eggshells that are discarded by the food industry. Transformation of this waste into a pharmaceutical excipient. The conversion of this waste into pharmaceutical excipients will result in cost savings as well as a new supply of raw materials for chewing gum production. The goal of this research is to create eggshell powder as a calcium ion excipient in the production of chewing gum. To obtain a contamination-free material, the egg shells were first ground in a blender and then sterilized in a 20-litre horizontal digital stermax autoclave for 45 min at 122°C. The egg shells were then ground into a fine powder in a Marconi MA 500 ball mill at 249 RPM for 12 hr. The ground eggshell powders were sieved in a No. 80 sieve (aperture of 0.177 mm) to limit particle size. Optical microscopy was used to examine the external morphology of powdered egg shells. Excipients improved the stability of the chewing gum while also enhancing medication release. The quicker the administration of the prescribed chewing gum begins, the better. Scanning electron microscopy was used to examine chicken eggshells with all excipients and pure eggshells (SEM). X-Ray Diffraction was used to examine the chicken eggshell with all excipients and pure eggshell (XRD). Pharmacopea specifications were used to evaluate all formulations. The pH, moisture content, viscosity, weight uniformity, And thickness tests were all within acceptable ranges.","PeriodicalId":413740,"journal":{"name":"Asian Journal of Biological and Life sciences","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133569574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Staining Capability of Plant Extracts for the Identification of Gram-positive and Gram-negative Bacteria: A Systematic Review","authors":"Rae Antoinette D. Badar, Junissa Lalaine R. Carmona, John Gabriel C. Collantes, Daneery R. Lojo, Sean Mairose B. Ocampo, Roxette Lorie C. Ursua, Daniel H Bercede","doi":"10.5530/ajbls.2022.11.37","DOIUrl":"https://doi.org/10.5530/ajbls.2022.11.37","url":null,"abstract":"Background: Gram Staining is an essential diagnostic procedure where bacteria are stained to be properly identified. However, the stains used were found to be toxic with carcinogenic properties and (insert may) potentially harm living organisms as well as the environment. Methods: To address this issue, plant extracts were utilized in different experiments to test their staining capability on the bacteria. This study provides a systematic review of online articles and studies related to the staining capabilities of various plants. Results: Following the inclusion and exclusion criteria, a total of 137 papers were reviewed and only 11 papers were included in the study. Both the presence of phytochemicals and the pH concentration influenced the staining capacity of most plant extracts. In the following studies that were examined, most of the plants were able to stain gram-positive bacteria in comparison to the gram-negative bacteria. Conclusion: It was found that oxidized aqueous (hot or cold) extract of Lawsonia inamis, methanolic extracts of Solanum melongena L., 100% concentration of ethanolic extracts of Ipomoea batatas , ethanolic extract of Clitoria ternatea , Pterocarpus osun extracts, Bixa orellana extracts, and Hibuscus sabderiffa extract were able to stain the bacteria. Meanwhile, certain extracts have poor staining capability namely Garcinia kola mesocarp , Vitex doniana fruit , Lantana aculaeta fruit , Cnestis ferrugnea fruit , and Pterocarpus soyauxii stem. It is recommended to find other alternative extracts along with various extraction methods and concentration levels that may further enhance the affinity of the stain. Anthocyanin The use of Ipomoea batatas extract with the concentrations of 60%, 70%, 80%, and 90% in staining gram-positive bacteria showed substandard results. The bacteria did not stain well as compared to using crystal violet dye. However, a 100% concentration of the extract was revealed to have a good staining capacity. On the other hand, all mentioned concentrations of the extract were able to stain gram-negative bacteria thoroughly.","PeriodicalId":413740,"journal":{"name":"Asian Journal of Biological and Life sciences","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128416861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Systematic Review on the Antihyperglycemic Property and Effect of Roselle (Hibiscus sabdariffa Linn.) Fruit Peel Extract in Diabetic Male Rats (Rattus norvegicus) Induced by Streptozotocin","authors":"Marycel Aison, Joshua Alvin Surisantos, Erin Anjella Chua, Ma. Danielle Kabiling, Kashima Mira Manalo, Edzyrel Ann Maravilla, Aldren Enrick Natividad, Winny Kyla Therese Medrano, E. A. A. Cano","doi":"10.5530/ajbls.2022.11.41","DOIUrl":"https://doi.org/10.5530/ajbls.2022.11.41","url":null,"abstract":"Aim/Background: Diabetes mellitus is a chronic disease that inhibits insulin production, which affects blood levels. Over-the-counter drugs regulate blood levels, while medicinal plants such as Roselle ( Hibiscus sabdariffa Linn.) are used as a remedy. This systematic review aims to establish the antihyperglycemic properties of Roselle fruit peel extract, determine its effectiveness in diabetic male rats ( Rattus norvegicus ) induced by Streptozotocin, and evaluate the efficiency of Roselle fruit peel extract compared to over-the-counter diabetes medication; A comprehensive and systematic search of (1) Antihyperglycemic property and effect of Hibiscus sabdariffa Linn., (2) Samples used are diabetic male rats (3) Orally induced Streptozotocin given by medical professionals. Materials and Methods: The data were gathered through different databases, such as PubMed Central, the IOSR Journal of Pharmacy, EBSCOHost, Google Scholar, and ResearchGate; out of 100 studies retrieved, only 13 were utilized due to the elimination of the 87 by exclusion criteria. Diabetes is ranked 6th as the highest cause of death in the Philippines, ranking 9th worldwide. Results and Conclusion: In lieu of this, herbal or more organic forms of remedy are pursued by people who have diabetes. The review of studies shows that the Hibiscus sabdariffa Linn. plant possesses hypoglycemic, hyperlipidemic, and antioxidative properties due to the phytochemicals present. This review also solidifies the notion that Roselle extract and over-the-counter drugs have the same effect on blood sugar levels upon rat experimentation.","PeriodicalId":413740,"journal":{"name":"Asian Journal of Biological and Life sciences","volume":"74 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127368697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of Serum and Salivary Lipid Profile in Control Subjects and Diabetic Patients–A Comparative Study","authors":"Nirmala Arunachalam, M. Sultana","doi":"10.5530/ajbls.2022.11.46","DOIUrl":"https://doi.org/10.5530/ajbls.2022.11.46","url":null,"abstract":"Patients with diabetes mellitus have a greater risk of dyslipidaemia. Abnormalities in lipid metabolism have been shown to be key risk factors for the development of diabetes-related complications. Lipids are the critical biomolecules for pathological and physiological processes in the human body and their examination is essential for diagnosing health and disease conditions such as diabetes mellitus, atherosclerosis and related complications. The objective of this study was to ascertain the diagnostic potential of saliva in the assessment of lipid profile as compared to that of serum lipid profile. The selected study area of Thiruporur conducted a Diabetic camp to collect the samples from 200 control subjects and 200 Type - II diabetic patients from different age groups. Total Cholesterol (TC), High-Density Lipoprotein (HDL), Triglycerides (TG), Low-Density Lipoprotein (LDL), Very Low-Density Lipoprotein (VLDL), and the ratio of Total Cholesterol to High-Density Lipoprotein (TC/HDL) were analysed in the serum and saliva of the diabetic patients and the control subjects. When compared to the control group, patients with Type II diabetes have an abnormal blood lipid profile with elevated TC (246.8253.48mg/dl), high LDL (156.6253.484mg/dl), VLDL (42.36510.99mg/dl), TG (211.833 54.97 mg/dl), and TC/HDL ratio (5.471 1.239), but inadequate HDL (45.2793.199 mg/dl) levels were observed. According to the findings of this study, an increase in serum lipid profile values and corresponding increases in saliva lipid profile values were also observed. As per the outcome of the present study, saliva can be utilised as a non-invasive diagnostic tool for measuring lipid profile in diabetic patients","PeriodicalId":413740,"journal":{"name":"Asian Journal of Biological and Life sciences","volume":"171 6","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132906289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of Multidrug-Resistant Salmonella spp. in Asia: A Mini-Review","authors":"Lara Julia R. Salvador, Leanne Wilfrede D. Intengan, Lheean Aira G. Castillo, Allyssa Dale D. Baluyut, Maria Ellaine Mae C. Camarao, Ariane De D. Rueda, Blessed Joy B. Baligod, Champagne P. Go, Mary Rose F. Lirio","doi":"10.5530/ajbls.2022.11.36","DOIUrl":"https://doi.org/10.5530/ajbls.2022.11.36","url":null,"abstract":"Salmonella is a known causative agent for food poisoning across the world with thousands of cases reported annually. The surging multidrug-resistant Salmonella was also evident throughout the decade, worsening the situation and making it an even more crucial public health burden . This paper aims to raise public health awareness on the rapid progression of Salmonella infection resulting from proliferating antimicrobial resistance in different Asian regions. A total of 50 articles extracted from Google Scholar, PubMed, NCBI, and ScienceDirect were included in this review. The prevalent Salmonella serotype and antibiotic that most isolates have built resistance to were identified per Asian region. Overall, Salmonella Typhi is identified as the most isolated typhoidal Salmonella strain while Salmonella Typhimurium is the prevalent NTS strain in Asia. Tetracycline is revealed to have the highest percentage of resistance among all isolated Salmonella spp. in conducted Asian studies. Alarming tetracycline resistance was observed in 11 out of 38 countries incorporated in this mini-review, this is due to chromosomal mutation and ribosomal binding site mutation. Most Salmonella isolates have inherited genetic elements with tetracycline-specific resistant genes, increasing the intrinsic mechanism for antimicrobial resistance. Following tetracycline, significant resistance against nalidixic acid and ampicillin was also observed. Thus, it is highly suggested for public health officials focus on improving the sanitary conditions in food production and processing together with water management safety to address the problem with Salmonella .","PeriodicalId":413740,"journal":{"name":"Asian Journal of Biological and Life sciences","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114209060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}