Nivedita Rajakumar, S. Sharda, S. Bhatt, Parth Shah, S. Murarka
{"title":"A Unique NIPT Discordance: FISH Comes to Rescue","authors":"Nivedita Rajakumar, S. Sharda, S. Bhatt, Parth Shah, S. Murarka","doi":"10.5530/ajbls.2023.12.28","DOIUrl":"https://doi.org/10.5530/ajbls.2023.12.28","url":null,"abstract":"Detection of mosaicism in prenatal genetic screening or diagnostic tests has always been a challenge both technologically and in predicting clinical outcomes. Riddled with concerns of false positives, confined mosaicism and other technological limitations, mosaicism can pose significant challenges in leading to an accurate diagnosis. Here we present a case where unique mosaicism was detected following a case of the high-risk non-invasive prenatal screening test (NIPT). Currently, as the most sensitive prenatal screening test, NIPT has proven consistency in even identifying mosaic cases of trisomies. Choosing the appropriate confirmatory test for a high-risk NIPT becomes all the more important for accurate counselling, diagnosis and taking further reproductive decisions.","PeriodicalId":413740,"journal":{"name":"Asian Journal of Biological and Life sciences","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129000832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mridusmita Dutta, N. Gogoi, B. Neog, K. Lekharu, Sarojmoni Sonowal, Himadree Phukon
{"title":"Morphology and Genetic Diversity of Garcinia cowa (Roxb.) in Upper Brahmaputra Valley of Assam","authors":"Mridusmita Dutta, N. Gogoi, B. Neog, K. Lekharu, Sarojmoni Sonowal, Himadree Phukon","doi":"10.5530/ajbls.2023.12.12","DOIUrl":"https://doi.org/10.5530/ajbls.2023.12.12","url":null,"abstract":"Background:Background: Garcinia cowa is a lesser-known species and it is therefore the limelight of this study to bring to notice its morphological and genetic diversity. Materials and Methods: Materials and Methods: The morphological diversity was studied using morphological markers and the genetic diversity was analyzed using 30 different primers for ISSR analysis. The DNA isolation in 2X and 4X buffer for PCR was done using the method given by Doyle and Doyle. The method was slightly modified. Results:Results: Tinsukia district showed the highest leaf length, leaf breadth and leaf vein. Dibrugarh district showed the highest leaf petiole. 4X buffer proved to be more effective lysis buffer in comparision to 2X buffer. Garcinia cowa showed 100% polymorphism with primers UBC-41 and UBC-42. Conclusion: Conclusion: Garcinia cowa was found to have considerable morphological diversity. 4X buffer proved to be more effective. The accessions of Garcinia cowa showed polymorphism only with primers UBC-40, UBC-41 and UBC-42 and the polymorphism percentage of Garcinia cowa was calculated which was found to be 100% with primers UBC-41 and UBC-42.","PeriodicalId":413740,"journal":{"name":"Asian Journal of Biological and Life sciences","volume":"39 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121065578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Avian Species Diversity in Different Habitats of Shivamogga, Karnataka, India","authors":"Saadath Ali Shairali, Krishna Naik Lokesh Naik","doi":"10.5530/ajbls.2023.12.22","DOIUrl":"https://doi.org/10.5530/ajbls.2023.12.22","url":null,"abstract":"Background:Background: Avifaunal diversity and its abundance measures the quality of the ecosystem. Diversified landscapes were assumed to furnish more niches or supportive resources which in turn helps to increase diversity of avifauna. Aim:Aim: To assess the diversity of avifauna and its status in different habitats of Shivamogga taluk. Materials and Methods: Materials and Methods: Field exploration for assessing the bird diversity was undertaken in the four different habitats of Shivamogga Taluk. Point count method and line transects was used for gathering data on abundance and diversity of birds in all the four different habitats. Results:Results: The present study was conducted in the study site for a period of one year. A total of 6,866 individual birds of 137 species representing 53 families and 18 orders was observed and 4 Near Threatened species and 3 Vulnerable species was recorded from the study area. Significant variation in avian species richness was observed from the four study sites (F=10.36, p <0.0001, df=5). Fisher alpha indices which consider both richness and abundance was higher in Site 1 (18.36) and lowest was observed Site 2 (11.32). The order Passeriformes dominated the bird community with 26 families and 57 species. Seven foraging guilds were observed in the study area. Insectivorous birds with 46 species were found to be dominant. Principal Component Analysis (PCA) was used to find the positive correlation between the Near threatened, Vulnerable species and the study sites. Conclusion:Conclusion: These findings implicate that the study area has a good number of avian species diversity. Each habitat has its own specialist species. Avian species richness was found to be higher in forest ecosystem while the dominance and abundance was more in Agrarian ecosystem. Alpha diversity was measured to comprehensively evaluate the quality of the different habitats.","PeriodicalId":413740,"journal":{"name":"Asian Journal of Biological and Life sciences","volume":"61 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126548003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Recent Advances in Herbal-Nano Formulation: A Systematic Review","authors":"Srinivasan N Srinivasan","doi":"10.5530/ajbls.2023.12.4","DOIUrl":"https://doi.org/10.5530/ajbls.2023.12.4","url":null,"abstract":"Plants have been used in the maintenance and protection of human health since ancient days and these medicinal plants play a dynamic role in preventing diseases. Natural compounds present in medicinal herbs were used to treat a bacterial infection, digestive disorders, respiratory illness, cardiac disorders, cancer, disorders in brain etc. Despite their outstanding therapeutic applications, the phytochemical components have limited solubility in water and thereby affecting bioavailability. In order to make effective use of this property and overcome limitations, novel strategies have been implemented in using medicinal herbs in health care. Nanotechnology has led to numerous scientific discoveries and invention of nano-medicine is tremendous. Nano-drug delivery is an attractive approach in the treatment of life-threatening diseases like cancer and neurological disorders as these nanoparticles could be linked with drugs to actively cross the blood-brain barrier. This review focusses on herbo-nano formulations and methods of delivering phytochemical drug to targeted cells. Nanoformulations have been implemented in research on delivering active herbal components, improving bioavailability and decrease toxic effects of diseases.","PeriodicalId":413740,"journal":{"name":"Asian Journal of Biological and Life sciences","volume":"188 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131748513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Study on Ovitrap Surveillance and Molecular Phylogeny of Dengue Vectors (Aedes aegypti and Aedes albopictus) based on Cytochrome C Oxidase Subunit I (COI) Genes from Different Habitat Types of North Eastern, India","authors":"Himadri Borah, D. Bora","doi":"10.5530/ajbls.2023.12.21","DOIUrl":"https://doi.org/10.5530/ajbls.2023.12.21","url":null,"abstract":"","PeriodicalId":413740,"journal":{"name":"Asian Journal of Biological and Life sciences","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129593302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Studies on Mycorrhizal Associations in an Orchid","authors":"L. K. Attri","doi":"10.5530/ajbls.2023.12.24","DOIUrl":"https://doi.org/10.5530/ajbls.2023.12.24","url":null,"abstract":"","PeriodicalId":413740,"journal":{"name":"Asian Journal of Biological and Life sciences","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114392448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Babitha Babitha MC, Gomathi Gomathi V, Uma Maheswara Reddy C, Kumar Kumar M, Venkateswarlu Venkateswarlu BS
{"title":"In vitro Neuroprotective and Antioxidant Studies on Extract of Woodfordia fruticosa Flowers and its Phytochemical Analysis","authors":"Babitha Babitha MC, Gomathi Gomathi V, Uma Maheswara Reddy C, Kumar Kumar M, Venkateswarlu Venkateswarlu BS","doi":"10.5530/ajbls.2023.12.7","DOIUrl":"https://doi.org/10.5530/ajbls.2023.12.7","url":null,"abstract":"Aim:Aim: Medical illnesses known as Neurodegenerative Diseases (NDDs) have generated considerable attention in the science-based community. The in vitro approach for determining the antioxidant potentials of W. fruticosa flowers and their neuroprotective effects. Materials and Materials and Methods:Methods: Woodfordia fruticosa flower extract samples that underwent GC-MS analysis. DPPH and ABTS Radical Scavenging assays were performed. The neuroprotective potential of the selected extract was assessed using IMR-32 by MTT assay. Results:Results: Twenty-one compounds were found in the methanolic leaf extract of Woodfordia fruticosa , according to the GC-MS analysis. Using the DPPH and ABTS assays, in vitro antioxidant activity was assessed. The standard and test samples produced various concentrations (12.5, 25, 50, 100, and 200 g/mL). Percentage suppression of the anti-oxidant action is shown. Ten distinct acetone extract compounds and nine different ethanol extraction components were found. Both preparations are more effective in scavenging free radicals as their concentration increases. Conclusion:Conclusion: The current research demonstrates W. fruticosa’s ability to protect neurons from Lipopolysaccharide-induced neurotoxicity in neuronal cells. Maximum cytotoxic effect was found in the MTT solubilization found better at high-end concentrations than at Lower concentrations among both extracts.","PeriodicalId":413740,"journal":{"name":"Asian Journal of Biological and Life sciences","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131246940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. Chaudhary, Raghavdeep Sharma, Sayali Rupanar, S. Jadhav, Atharva Bongade, P. Shinde, Snehal Gavit
{"title":"Preparation and Evaluation of Herbal Mouthwash Containing Hydroalcoholic Extract of Pongamia pinnata","authors":"P. Chaudhary, Raghavdeep Sharma, Sayali Rupanar, S. Jadhav, Atharva Bongade, P. Shinde, Snehal Gavit","doi":"10.5530/ajbls.2023.12.23","DOIUrl":"https://doi.org/10.5530/ajbls.2023.12.23","url":null,"abstract":"Aim:Aim: To formulate and evaluate an herbal mouthwash containing hydroalcoholic extract of Pongamia pinnata . The oval shaped oral cavity contains surfaces covered with bacteria such as Streptococcus mutans , Porphyromonas gingivalis , Staphylococcus and Lactobacillus that causes various tooth problems such as decay, gingivitis, sensitive teeth, root infection, bad breath and enamel erosions. The role of herbal plants is significant in the development of many oral formulations. Materials and Methods: Materials and Methods: Phytochemical screening of the above-mentioned plant was done. Leaf extraction was carried out for preparation of mouthwash. Formulation and evaluation of all three formulations was done. Results:Results: Preliminary phytochemical screening of hydroalcoholic leaves extract of Pongamia pinnata confirmed the presence of phenolic compounds, tannins, flavonoids, terpenoids and alkaloids. Three formulations of mouthwash were prepared by using Pongamia pinnata , mint oil, PEG 40, glycerol, benzoic acid etc. and evaluated for different parameters like colour, odour, pH, viscosity, turbidity and stability studies. Conclusion:Conclusion: In comparison to chemical products, herbal drugs are widely regarded as highly effective. The goal of the present research was to formulate and evaluate an herbal mouthwash. So, in this research article we have focused on Pongamia pinnata (Common name Pongame oil tree) plant that belongs to Fabaceae family. Herbal mouthwashes can be used in combination with other oral hygiene practices like brushing and flossing. Due to their effectiveness against oral pathogens, herbal mouthwashes are high in demand because it provides immediate pain relief and has few side effects.","PeriodicalId":413740,"journal":{"name":"Asian Journal of Biological and Life sciences","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130898788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Phytochemical Evaluation and Metabolic Profiling of Methanolic Extracts from the Stem and Leaf of Suregada multiflora (A. Juss.) Baill.: An Ethnobotanically Important Medicinal Plant","authors":"Usha Usha SS","doi":"10.5530/ajbls.2023.12.5","DOIUrl":"https://doi.org/10.5530/ajbls.2023.12.5","url":null,"abstract":"Aim:Aim: In Indian traditional knowledge; many plants are documented for their therapeutic usage. Secondary metabolites are responsible for this pharmacological potentiality. Suregada multiflora , known as false lime, is used traditionally for the treatment of many diseases. Therefore, the present study was undertaken to evaluate the phytochemical profile of stem and leaf methanolic extracts from S. multiflora to substantiate their therapeutic efficacy. Materials and Methods: Materials and Methods: Physicochemical evaluation was undertaken to check its usage in crude drug formulations; it was followed by the phytochemical analysis of the stem and leaf using non-polar to polar solvents. Methanolic extracts of stem and leaf were subjected to HPTLC analysis, and to identify the compounds, LCMS analysis was carried out. Results:Results: Preliminary phytochemical analysis showed that the methanolic extract of stem and leaf comprises a remarkable number of phytoconstituents. It was further substantiated with HPTLC analysis in terms of the total number of peaks, peak heights, peak area, percent area and R f values. Following this, LC-MS analysis was conducted, and positive ionization of the leaf revealed 47 components, while the negative mode showed 60 identified molecules. Similarly, the positive ionization of the stem showed 41 components, and the negative ionization of the stem showed 58 identified components. Conclusion:Conclusion: Methanolic extract of S. multiflora stem and leaf contains a pool of phytochemicals which might be accountable for its therapeutic value and justifies its traditional usage.","PeriodicalId":413740,"journal":{"name":"Asian Journal of Biological and Life sciences","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133175725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}