Asian Journal of Biological and Life sciences最新文献

{"title":"A Study on Callus Induction and the Successful Regeneration of Plantlets from Rhizome Explants of Alpinia galanga (L.) Willd., a Valuable Medicinal Plant","authors":"Kizhakke Modongal Shamsudheen, Valiyaparambath Musfir Mehaboob, K. Faizal, Palusamy Raja, G. Thiagu, A. Shajahan","doi":"10.5530/ajbls.2023.12.68","DOIUrl":"https://doi.org/10.5530/ajbls.2023.12.68","url":null,"abstract":"Aim:Aim: The primary focus of our study is to establish a reliable approach for plant regeneration via indirect organogenesis of Alpinia galanga , utilizing rhizome buds. Materials and Methods: Materials and Methods: The rhizome explants were placed onto MS media supplemented with 0.5 to 2.5 mg/L 2,4-D to induce callus formation. The more effective callus induction was observed on MS media containing 1.5 mg/L 2,4-D with a light green, compact nature of callus. The callus transferred to MS media supplemented with various cytokinin’s such as BAP, Kinetin and TDZ in different concentrations for shoot induction. Results: Results: The highest shoot induction frequency (91.0±3.21%), the maximum number of shoots per callus (9.66±0.88) and the highest plantlet length (91.13±1.44 mm) were observed in 1.0 mg/L BAP. The best-regenerated shoot buds were then transferred to auxins such as NAA and IBA with various concentrations (0.25 mg/L, 0.5 mg/L, 0.75 mg/L and 1.0 mg/L) for root initiation. High rooting frequency (93.00±1.73%), maximum number of roots (21.66±1.76) and maximum root length (93.33±1.76 mm) were induced in MS medium containing 0.5 mg/L IBA. Conclusion: Conclusion: We have developed a reliable and easily replicable protocol for callus induction and subsequent plant regeneration of A. galanga in controlled laboratory settings. Following this, the in vitro plantlets were successfully acclimatized to field conditions.","PeriodicalId":413740,"journal":{"name":"Asian Journal of Biological and Life sciences","volume":" 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140386562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation of Lactobacilli from Freshly Drawn Raw Milk of Desi and Crossbred Cows of Gurugram Region, Haryana","authors":"Prachi Prachi P, Sandhya Khunger, Sunil Kumar, Mukesh Sharma, Mukul Mudgal","doi":"10.5530/ajbls.2023.12.82","DOIUrl":"https://doi.org/10.5530/ajbls.2023.12.82","url":null,"abstract":"Background: Background: Cow milk is of great concern these days because of its high nutritional value as well as the probiotic flora present in it, which confer several benefits to the human body. Lactobacillus is one such beneficial bacterium present in milk that helps the human body against allergic reactions, gastrointestinal diseases, and irritable bowel. Aim:Aim: The current study aims to determine the prevalence and isolation of Lactobacillus present in milk. The raw cow milk samples were collected from two categories of cows: desi cows and cross-breed cows. Raw cow milk is said to be harmful for human consumption due to the presence of pathogenic microflora. Materials and Materials and Methods: Methods: The raw cow milk samples were cultured on MRS agar (selective media) to isolate the diversity of Lactic acid bacteria (LAB) present in cow milk as probiotic flora. A total of twelve milk samples were taken; 5 from the cross-breed cows and 7 from the desi cows. Samples were cultured on MRS agar anaerobically for 24-48 hours. The bacterial isolates were tested for morphological characters, and biochemical tests including catalase, oxidase, citrate, sulfide, indole motility, and tolerance at physiological conditions like growth at different temperatures (ranging from 25-450C), growth in different saline concentrations (2%, 4%, 6%, 8%, and 10%), and growth in different pH (4, 5, 6, 7, 8). Result: Result: At the end of the study, three isolates were found gram-positive rods, out of which, 2 were catalase-negative and one was catalase-positive. All three strains were able to tolerate some physiological conditions. Conclusion: Conclusion: These can be further used as preservatives in some food items to increase their nutritional value as well as for further molecular studies for the identification of particular Lactobacillus species. Some new strategies should be implemented to make more efficient identification of LAB strains.","PeriodicalId":413740,"journal":{"name":"Asian Journal of Biological and Life sciences","volume":" 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140387103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial Activity of Water Soluble and Fat- Soluble Vitamins against Drug Resistance Clinical Bacteria Klebsiella Pneumonia","authors":"Afrah Abdul Kader, Alfiya Azeez, Ayifa Mahjabin, Fairos Babu, Farha Madathil Mikacha, Fathima Shabeena, Fida Fathima, Fidha Sulthana, Hameena Hanna, Hanoona Parambatt, Jumni Ambalavan Puthan Peediyakkal, Lafa Shihab, Mohammed Abdul Kareem, Muhammed Nihad, Munawvara Fathima, Nadeena Sherin, Nandhana Dineeshan Sinitha, Nida Sherin, Mohamed Nafees, Ruqaya Mustafa, Shahana Sini, Shahla Thasni, Sreeshna Pallath, Vafeena Mariyam, Abdul Hannan Shaikh, Shilpa Shilpa VP, Sirajudheen Mukriyan Kallungal, Muddukrishnaiah Kotakonda","doi":"10.5530/ajbls.2023.12.67","DOIUrl":"https://doi.org/10.5530/ajbls.2023.12.67","url":null,"abstract":"Background:Background: Vitamins play an important role in enhancing immunity, which helps the body fight various infections. Aim:Aim: This study investigates the antimicrobial activity of Biotin (Vitamin B7) and Ascorbic Acid (Vitamin C) against clinically significant microorganisms, specifically Klebsiella pneumoniae . We explored their potential as agents for disrupting protein biotinylation and the mechanisms underlying their antimicrobial effects. Materials and Methods: Materials and Methods: Clinical microorganisms were obtained from the Clinical Microbiology Lab in Coimbatore, India and cultured to match the MacFarland standard turbidity. The Kirby-Bauer Method was used to assess the antimicrobial activities of Biotin, Ascorbic Acid, and other vitamins against K. pneumoniae . The Minimum Inhibitory Concentrations (MIC) of Biotin and Ascorbic Acid were determined using a serial dilution method. Transmission Electronic Microscopy (HR-TEM) was used to visualise the antimicrobial effects of Biotin and Ascorbic Acid on K. pneumoniae . Results:Results: The results showed antimicrobial activity of Biotin and Ascorbic Acid against K. pneumoniae , with zones of inhibition of 14 and 12 mm, respectively. The MIC values for Biotin and Ascorbic Acid were 62.5 and 125 µg/mL, respectively. HR-TEM analysis revealed significant morphological alterations in the treated bacterial cells compared with those in untreated cells. Conclusion:Conclusion: This study provides insights into the antimicrobial potential of Biotin and Ascorbic Acid, and highlights their mechanisms of action. Unlike traditional antibiotics, these vitamins demonstrate promising antimicrobial effects and have potential applications as combination therapies or adjuncts to conventional treatments.","PeriodicalId":413740,"journal":{"name":"Asian Journal of Biological and Life sciences","volume":" 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140386497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elevated Temperature and Carbon Dioxide alter the Tea Rhizosphere Soil Dynamics","authors":"A. S. Rahman, Jayanta Barukial, Satya Ranjan Sarmah, R. D. Baruah","doi":"10.5530/ajbls.2023.12.76","DOIUrl":"https://doi.org/10.5530/ajbls.2023.12.76","url":null,"abstract":"Aim: Aim: The aim of this work is to explore the impact of climate change on the tea rhizosphere soil dynamics. The experiment sought to study the tea rhizosphere Soil Organic Carbon (SOC), Total Nitrogen (TN), Microbial Biomass Carbon (MBC) and Microbial Biomass Nitrogen (MBN) under elevated levels of temperature and Carbon Dioxide (CO 2 ). Materials and Methods: Materials and Methods: The experimental design was set up with two Open Top Chamber (OTC) facilities, creating localized conditions with increased temperature and CO 2. Both temperature and CO 2 were elevated in OTC-1 (eTemp+eCO 2 ), with CO 2 concentration of 550 ppm, while in OTC-2 (eTemp), only the temperature was elevated. Temperature was 1.5-2ºC higher than ambient. Four tea cultivars (TV1, TV20, TV22 and TV23) were placed inside the OTCs and rhizosphere soil samples were collected at regular intervals. Results: Results: The results indicated that in eTemp+eCO 2 treatment, over the period of time, the rate of change in SOC showed significant increase ( p< 0.01 ) compared to control, while TN showed no significant variations, the rate of change in MBC showed significant increase ( p< 0.05 ) in eTemp treatment. No significant variation was observed in the rate of change in MBN. Overall, SOC and MBC, after 300 hr of treatments showed significant increase ( p <0.001) in both eTemp and eTemp+eCO 2 treatments when compared to control. However, after 300 hr of treatment overall soil TN exhibited a significant increase ( p <0.05) in eTemp+eCO 2 treatment. Conclusion: Conclusion: This study addresses the research gap through an evidence based experimental work that monitors the impact of temperature and CO 2 on tea rhizosphere. From the study, it can be concluded that elevated temperature and CO 2 alter the rate of change in soil organic carbon pool of tea rhizosphere soil along with the accumulation of biomass carbon, whereas no such alteration was found for microbial biomass nitrogen.","PeriodicalId":413740,"journal":{"name":"Asian Journal of Biological and Life sciences","volume":" 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140386706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Compost Preparation from Different Organic Wastes: Their Biochemical Analysis and Effect on Growth of Bottle Gourd","authors":"T. Taneja, Indu Sharma, Mukesh Kumar, Mahesh Kumar Rana, Raj Singh","doi":"10.5530/ajbls.2023.12.62","DOIUrl":"https://doi.org/10.5530/ajbls.2023.12.62","url":null,"abstract":"Aim:Aim: A study was carried out to prepare from the different organic wastes and to assess their effect on bottle gourds. Background:Background: Composting is a crucial agricultural process that aids in the recycling of agricultural and farm wastes. Beneficial microbes and plant nutrients are abundant in high-quality compost. It is a natural process to break organic matter, like leaves and food scraps, into useful manure, which is then used as manure to enrich the soil with carbon and nitrogen, phosphorus, potassium and especially micronutrients, essential for the growth of the plants. Materials and Methods: Materials and Methods: Four types of compost were prepared from (i) leaves (ii) fruits and vegetables, waste (iii) cow dung 2 years old (iv) cow dung 4 years old which were analyzed for pH, electrical conductivity, moisture content, bulk density and ash content. These composts were also analyzed for nitrogen, phosphorus and potassium content. Results:Results: Out of the four composts, the compost prepared from leaves had the maximum pH (7.69), electrical conductivity (8.44 S/m), moisture content (55%) and bulk density (0.57 g/cm 3 ) as compared to the rest of the three composts. Compost prepared from fruits and vegetable wastes had the maximum concentration of nitrogen (1.5%), potassium (1.2%) and phosphorus (0.8%) as compared to compost prepared from leaves, vegetables and cow dung. The study also revealed the significantly positive effect of composts on vine length and number of leaves per plant of bottle gourd ( Lagenaria siceraria ) 10, 15, 30, 50 and 70 days after the application of composts. Conclusion:Conclusion: Composting recycles a variety of organic materials, which would otherwise be considered garbage. These processes play a significant role in sustainable agriculture and organic farming.","PeriodicalId":413740,"journal":{"name":"Asian Journal of Biological and Life sciences","volume":" 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140387075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Red Grape Seed Extract (RGSE) declines Neuronal and Oxidative Damage in the Brain Regions of Alzheimer’s Induced Wistar Rats.","authors":"Thulasi M Thulasi, Janardhana K Janardhana, Aruna C Aruna, M. M. Naik, A. G. Naik, V. U. Kiran","doi":"10.5530/ajbls.2023.12.72","DOIUrl":"https://doi.org/10.5530/ajbls.2023.12.72","url":null,"abstract":"Objectives:Objectives: This research explores Red Grape Seed Extract ( RGSE ) declines Neuronal and Oxidative Damage in the brain regions of Alzheimer’s Induced Wistar Rats. Materials and Methods: Materials and Methods: A cohort of three-month-old Wistar rats were divided into two groups, receiving either a standard diet (control diet) or a diet supplemented with 2% RGSE over a 60-day period. RGSE, containing 592.5 mg/g dry weight of Total Phenolic Content (TPC), consisted of Gallic acid (49 mg/g), Catechin (41 mg/g), Epicatechin (66 mg/g), and Proanthocyanidins (436.6 mg catechin equivalents/g). Results:Results: Long-term RGSE diet feeding proved well-tolerated, exhibiting no fatalities or behavioral abnormalities. Furthermore, no irregularities in food consumption or body weight were observed. The levels of Amyloid-beta (Aβ) in the brains of Wistar rats subjected to RGSE were notably lower compared to Alzheimer ‘s-induced Wistar rats on the control diet. Additionally, RGSE administration led to a reduction in amyloid plaques and microgliosis in the brains of Alzheimer’s-induced Wistar rats. Conclusion:Conclusion: The polyphenol component of RGSE demonstrated a substantial decrease in brain Aβ load and microglia activation. Ultimately, polyphenol-rich RGSE exhibited the potential to inhibit Aβ deposition and alleviate neuronal and oxidative damage in the Wistar rat model, suggesting its promise in delaying the progression of Alzheimer’s disease.","PeriodicalId":413740,"journal":{"name":"Asian Journal of Biological and Life sciences","volume":" 15","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140386290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of in vitro Anti-Inflammatory Activity of Leaves of Terminalia chebula Retz. (Combretaceae)","authors":"Soumya Jose, S. Thankappan","doi":"10.5530/ajbls.2023.12.73","DOIUrl":"https://doi.org/10.5530/ajbls.2023.12.73","url":null,"abstract":"Aim:Aim: The present study focused to establish the in vitro anti-inflammatory activity of leaf extracts of Terminalia chebula prepared using different solvents. Materials and Methods: Materials and Methods: The in vitro anti-inflammatory potentiality of leaves of Terminalia chebula were tested using the protein denaturation inhibition assay (bovine serum albumin inhibition assay). Results:Results: Leaves of Terminalia chebula were extracted by ultrasonic assisted extraction using different solvents in increasing order of polarity. The extracts so obtained were screened for anti-inflammatory potential. Leaf extracts of different concentrations ranging from 6.25-100 µg/mL were incubated with bovine serum albumin and its ability to inhibit protein denaturation was measured. The percentage of inhibition of standard diclofenac sodium was determined and its IC 50 value was found to be 49.57 µg/ mL. The ethanol, ethanol: distilled water extracts (4:1, 3:2, 2:3, 1:4) and distilled water extracts exhibited greater inhibition of protein denaturation and their IC 50 values ranged from 52.03 µg/mL to 77.75 µg/mL. Conclusion:Conclusion: The current study proved that leaves of Terminalia chebula exhibited considerable amount of anti-inflammatory activity and could be used as anti-inflammatory drug but only after proper scientific validation (further in vitro and in vivo studies).","PeriodicalId":413740,"journal":{"name":"Asian Journal of Biological and Life sciences","volume":" 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140386855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical Analysis and in vitro Antioxidant Activities of Three Species of Premna L. from Kerala","authors":"Harikrishnan Harikrishnan MR, S. Beevy","doi":"10.5530/ajbls.2023.12.70","DOIUrl":"https://doi.org/10.5530/ajbls.2023.12.70","url":null,"abstract":"Aim:Aim: Premna, a genus belonging to the family Lamiaceae, is known for its immense bioactivity and has a significant role in traditional medicine and modern pharmacological research. The present study sought to look into the bioactive compounds present in the methanolic extracts and the antioxidant activities of three selected species of Premna from different localities of Kerala State, India. Materials and Methods: Materials and Methods: This study offers a thorough comparison of the phytochemical composition, antioxidant activity, and Gas Chromatography-Mass Spectrometry (GC-MS) profiling of three species of Premna such as Premna scandens Roxb., Premna mollissima Roth. Premna glaberrima Wight. from Kerala, India. Results: Results: Qualitative analysis revealed the presence of major phytochemical compounds such as alkaloids, phenolics, flavonoids, terpenoids and steroids in the three species and the quantitative assays indicated considerable differences in their concentrations in these species. The antioxidant activity experiments revealed varied degrees of free radical scavenging ability, which corresponded to the observed phytochemical diversity. Several bioactive compounds were identified by GC-MS investigation of three different Premna species. Among them P. glaberrima, showed peaks indicating the presence of 22 compounds, whereas P. scandens and P. mollisima were confirmed with the presence of 17 and 16 compounds respectively some of which may contribute to the reported antioxidant activity. Conclusion: Conclusion: Thus, a variety of phytochemicals present in the three Premna species can potentially enhance their antioxidant activity. The ability to neutralize free radicals and lessen oxidative stress was indicated by the antioxidant capacity measured using the DPPH, nitric oxide scavenging, and superoxide scavenging procedures.","PeriodicalId":413740,"journal":{"name":"Asian Journal of Biological and Life sciences","volume":" 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140386382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Hematological Profile of Three Species of Schizothorax Group Found in the Streams of Himalayan Region","authors":"Siddiqui Siddiqui U, Rani Rani A, Bisht Bisht HCS, Pandey Pandey NN","doi":"10.5530/ajbls.2023.12.81","DOIUrl":"https://doi.org/10.5530/ajbls.2023.12.81","url":null,"abstract":"Assessing hematological parameters offers a comprehensive evaluation of the health condition of fish. The present study evaluated the variations in hematological parameters, including Total Eryth - rocyte Count (TEC), Total Leukocyte Count (TLC), Hemoglobin (Hb), Hematocrit (Hct), Mean Corpus - cular Volume (MCV), Mean Corpuscular Hemoglobin (MCH), and Mean Corpuscular Hemoglobin Concentration (MCHC), in three species of Schizothorax fish (S. richardsonii, S. plagiostomus, and S. progastus ) . The hematocrict values of the three species differed significantly ( S. richardsonii 25.5±2.3 has the lowest hematocrit value compared to S. progastus 27.5±2.6 and S. plagios-tomus 29±4.6). S. plagiostomus had considerably greater Hemoglobin (Hb) and Red Blood Cell count (RBC) levels compared to S. richardsonii and S. progastus. The results revealed significant ( p <0.05) variations among the three species. The MCHC value ranged between 34.1%, 35.0%, and 35.9% indicating an insignificant ( p >0.05) difference. Therefore, the baseline data produced during this study can be efficiently utilised to monitor the health condition of coldwater fishes in order to preserve them sustainably in aquatic systems under the scenario of climate change.","PeriodicalId":413740,"journal":{"name":"Asian Journal of Biological and Life sciences","volume":" 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140386518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of Bioactive Compounds in Acetone Leaf and Stem-Bark Extracts of Psychotria dalzellii Hook.f. by GC-MS Analysis and Evaluation of in vitro Anti-bacterial Properties","authors":"Aralahalli Borappa Thejashree, Raja Naika","doi":"10.5530/ajbls.2023.12.66","DOIUrl":"https://doi.org/10.5530/ajbls.2023.12.66","url":null,"abstract":"Background: Background: Psychotria dalzellii Hook.f. (Rubiaceae) is an ethnobotanically important plant species, traditionally the stem part is used as a remedy for pruritus and the leaf juice is utilized in the preparation of eye drops for migraines. However, these claims lack of scientific validation. The current study focused on Gas Chromatography-Mass Spectrometry analysis and in vitro evaluation of antibacterial properties of leaf and stem bark acetone extracts of P. dalzellii. Materials and Methods: Materials and Methods: P. dalzellii leaf and stem-bark samples were collected from Joida, Western Ghats, Karnataka, India. The collected plant materials were subjected to Soxhlet extraction using acetone. The presence of phytochemicals were investigated by preliminary qualitative screening and GC-MS for quantitative estimation. Then, the antibacterial potential was analysed by agar well diffusion technique against selected plant and animal pathogenic bacterial strains. Results:Results: GC-MS chromatogram of acetone stem-bark extract shows the presence of 15 chemical constituents in that, Resorcinol (58.36%) and Hexadecanoic acid, 2-hydroxy-1-(hydroxymethyl) ethyl ester (8.81%) were found to be the major phytoconstituents. Whereas, the leaf extract shows 19 bioactive compounds in that, 3,7,11,15-Tetramethyl-2-hexadecen-1-ol (26.99%); Hexadecanoic acid, 2-hydroxy-1-(hydroxymethyl)ethyl ester (21.99%) and Resorcinol (17.87%) were found to be the major compounds. P. dalzellii extracts of stem-bark and leaf exerted greater antibacterial activity towards all tested bacterial strains. Among leaf and stembark extracts, the stembark acetone extract were shown to be the most potent against Gram-negative pathogenic bacterial strains like Pseudomonas syringae (19.33 ± 0.16 mm), Klebsiella pneumoniae (19.25 ± 0.14 mm), Xanthomonas compestris (18.16 ± 0.16 mm) and Escherichia coli (17.25 ± 0.14 mm). Conclusion:Conclusion: This study provides new insights into the Psychotria dalzellii as a potential tool for antibacterial drug discovery through in vitro investigation that could be used to treat human and plant infectious diseases.","PeriodicalId":413740,"journal":{"name":"Asian Journal of Biological and Life sciences","volume":" 17","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140386637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}