TAP CHI SINH HOC最新文献_第5页

Distribution and descriptive characteristics of the two giant pill millipedes of the genus Sphaerobelum Verhoeff, 1924 (Diplopoda, Sphaerotheriida, Zephroniidae) in Vietnam 文章标题1924年越南球形千足虫属两种巨型丸足虫的分布及描述特征(双足目，球形千足虫科，球形千足虫科)

TAP CHI SINH HOC Pub Date : 2019-03-28 DOI: 10.15625/0866-7160/V41N1.11400

N. Anh, P. Sierwald

引用次数: 0

Evaluation of the accumulation of organochlorine pesticides (ocps) in Bostrychus sinensis fish growing the soai rap estuary - Ho Chi Minh city 胡志明市soai rap河口养殖中华白鱀豚鱼体内有机氯农药(ocps)积累评价

TAP CHI SINH HOC Pub Date : 2019-03-28 DOI: 10.15625/0866-7160/V41N1.13358

Nguyen Xuan Tòng, T. T. Huong, M. Huong, D. T. Thủy, H. Luc

引用次数: 1

Transformation of E. coli modified gdha gene into Nicotiana tabacum 大肠杆菌修饰gdha基因在烟草中的转化

TAP CHI SINH HOC Pub Date : 2019-03-28 DOI: 10.15625/0866-7160/V41N1.10588

P. Hằng, N. T. Linh, Lê Bắc Việt, N. T. Lien, L. T. Hien, H. Hue

{"title":"Transformation of E. coli modified gdha gene into Nicotiana tabacum","authors":"P. Hằng, N. T. Linh, Lê Bắc Việt, N. T. Lien, L. T. Hien, H. Hue","doi":"10.15625/0866-7160/V41N1.10588","DOIUrl":"https://doi.org/10.15625/0866-7160/V41N1.10588","url":null,"abstract":"The bacterial gdhA gene encodes NADH-GDH enzyme. The gdhA-transgenic tobacco plants exhibited high growth performance and enhanced herbicide resistance as well as drought tolerance. In addition, the gdhA gene when expressed in maize plants could increase productivity because of improving stress tolerance. In this study, we isolated and analyzed a gdhA gene from the E. coli strain JM109. Then, the E.coli derived-gdhA sequence was modified by using OptimumGene™ software for plant compatibility. The optimized gdhA gene was inserted into an expression cassette containing the CaMV 35S promoter and the 35S terminator of the pRTRA7/3 vector. The 35S::gdhA::35S construct was ligated into the plant expression plasmid pCAMBIA1300 which then introduced into the A. tumefaciens strain EHA105. In order to examine the expression of the gdhA gene, we created gdhA-transgenic plants via Agrobacterium-mediated transformation into N. tabacum K326. The PCR analysis showed that two out of 20 plants were positive to the presence of the gdhA gene. The RT-PCR experiment also revealed that gdhA was expressed in transcriptional level. These results suggested that the gdhA expression construction can be transformed into other crops such as maize. ","PeriodicalId":409863,"journal":{"name":"TAP CHI SINH HOC","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121010930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic variation and outcrossing rate of Dipterocarpus dyeri in the Tan Phu tropical rain forest (Dong Nai), Vietnam 越南同奈檀富热带雨林双掌木的遗传变异和异交率

TAP CHI SINH HOC Pub Date : 2019-03-28 DOI: 10.15625/0866-7160/V41N1.10639

N. M. Duc, V. D. Duy, Dang Phan Hien, T. Thanh, N. Trang, N. M. Tam

{"title":"Genetic variation and outcrossing rate of Dipterocarpus dyeri in the Tan Phu tropical rain forest (Dong Nai), Vietnam","authors":"N. M. Duc, V. D. Duy, Dang Phan Hien, T. Thanh, N. Trang, N. M. Tam","doi":"10.15625/0866-7160/V41N1.10639","DOIUrl":"https://doi.org/10.15625/0866-7160/V41N1.10639","url":null,"abstract":"Dipterocarpus dyeri (Dipterocarpaceae) is widely distributed in lowland rainforests in southeastern Vietnam. Due to over- exploitation and habitat destruction in the 1980s and 1990s, this species is listed as threatened. Understanding the genetic variation and mating rate among D. dyeri population that occurs in forest patches is necessary to establish effectively conservation strategies for this species. To conserve the species in tropical forests, genetic diversity and mating rate were investigated using eight microsatellites (single sequence repeat, SSR) as markers. All of the eight loci were polymorphic. A total of 36 different alleles were observed across the loci screened. The SSR data indicated high genetic diversity (NA = 4.5; HO = 0.542 and HE = 0.667) and high inbreeding value, FIS= 0,182. The mating system parameters were determined using the mixed mating model and the results indicated high outcrossing rate (tm=0.81 and ts=0.675) and lowselfing rate (0.19). Difference of tm-ts value indicated that inbreeding contributed to selfing rate for this species in the Tan Phu tropical rain forest. The results reflected that D. dyeri habitat in this area has been restored and the number of individuals was high, about 500 individuals. However, seedlings were not found so far in this study. These results indicated the importance of conserving of the genetic resources of Dipterocarpus dyeri species in the Tan Phu rain forest. The conservation strategy should include an establishment of an ex-situ conservation site with new big population for this species from all genetic groups, which might improve its fitness under different environmental stresses. ","PeriodicalId":409863,"journal":{"name":"TAP CHI SINH HOC","volume":"44 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132829780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of the recombinant single chain variable fragments recognizing blood antigen fused with thioredoxin in Escherichia coli 硫氧还蛋白融合血抗原重组单链可变片段在大肠杆菌中的表达

TAP CHI SINH HOC Pub Date : 2019-03-28 DOI: 10.15625/0866-7160/V41N1.10924

D. Ha, L. Hong, T. N. Hải

引用次数: 0

Distribution of and the taxonomic keys to the species in the genus Daptonema Cobb, 1920 in Vietnam 标题越南水蚤属的分布及分类键

TAP CHI SINH HOC Pub Date : 2019-03-28 DOI: 10.15625/0866-7160/V41N1.13016

N. D. Tu, N. Thanh

引用次数: 2

Effects of potassium dichromate on the survival and reproduction of Moina micrura Kurz. 1875 (Cladocera: Moinidae) 重铬酸钾对古蚊(Moina microura Kurz. 1875)存活和繁殖的影响

TAP CHI SINH HOC Pub Date : 2019-03-28 DOI: 10.15625/0866-7160/V41N1.12568

T. Mau, L. V. Trang, N. T. N. Trinh, Tran Ngoc Son, Vo Van Minh

引用次数: 1

Cladoceran diversity in Vinh Long province and redescription of the rare species Grimaldina brazzai richard, 1892 (macrotricidae, anomopoda, branchiopoda) for zooplankton fauna Vietnam 越南永隆省枝足类的多样性及珍稀种Grimaldina brazzai richard, 1892(大桡足科，anomopod, branchiopod)的重新描述

TAP CHI SINH HOC Pub Date : 2019-03-28 DOI: 10.15625/0866-7160/V41N1.8609

L. T. Nga, D. Phan

{"title":"Cladoceran diversity in Vinh Long province and redescription of the rare species Grimaldina brazzai richard, 1892 (macrotricidae, anomopoda, branchiopoda) for zooplankton fauna Vietnam","authors":"L. T. Nga, D. Phan","doi":"10.15625/0866-7160/V41N1.8609","DOIUrl":"https://doi.org/10.15625/0866-7160/V41N1.8609","url":null,"abstract":"In a total of four surveys at eight sites in some major rivers in Vinh Long province during 2013 to 2014, 18 species of cladoceran, belonging to 14 genera, 7 families and 2 orders, were recorded. The cladocerans fauna in Vinh Long province is recognized as moderate diverse, and the species recorded were mostly common species. Among 18 species, Grimaldina brazzai Richard, 1892 is a rare species. A total of four females specimens of species G. brazzai were collected at the Co Chien river, but male specimens were absent. The specimens were morphologically identified as G. brazzai described first by Richard (1892), followed by Brook (1959), Idris (1983), Smirnov (1992) and Hollwedel (2003). Grimaldina brazzai is currently the only one species in the genus Grimaldina and has been recorded in several tropical countries, such as Brazil, Thailand, Laos, Philippines, Indonesia, Malaysia, Cambodia and Singapore. They occur in lakes and rice fields, but population of this species is very limited in nature. In Vietnam, this species was first recorded in South Vietnam by Shirota (1966), but has not been described since then until recently Sinev & Korovchinsky (2013) recorded this species in the Cat Tien National Park, Dong Nai province, South Vietnam. ","PeriodicalId":409863,"journal":{"name":"TAP CHI SINH HOC","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114923195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antioxidant, anti-inflammatory and hepatoprotective activities on carbon tetrachloride - induced hepatic damage in mice of trang to (ixora duffii) leaf extract 对四氯化碳致小鼠肝损伤的抗氧化、抗炎和保肝作用

TAP CHI SINH HOC Pub Date : 2019-03-21 DOI: 10.15625/0866-7160/V41N1.12734

Phan Kim Dinh, Nguyen Trong Tuan, D. Trang

{"title":"Antioxidant, anti-inflammatory and hepatoprotective activities on carbon tetrachloride - induced hepatic damage in mice of trang to (ixora duffii) leaf extract","authors":"Phan Kim Dinh, Nguyen Trong Tuan, D. Trang","doi":"10.15625/0866-7160/V41N1.12734","DOIUrl":"https://doi.org/10.15625/0866-7160/V41N1.12734","url":null,"abstract":"Trang To (Ixora duffii) belongs to the genus Ixora in the family Rubiaceae. The in vitro total antioxidant and anti-inflammatory capacity of the methanol extract of Trang To leaf was investigated using the phosphomolypdenum method and the bovine serum albumin denaturation test, respectively. The results showed that the methanol extract of Trang To leaf possessed relatively high antioxidant activity (OD0,5 = 15,551 ± 0,344 μg/mL) compared to that of the standard, trolox (OD0,5=2,315±0,083 μg/mL). The anti-inflammatory effect using bovine serum albumin denaturation test showed that the activity of the methanol extract of Trang To leaf (EC50= 6,03±0,12 μg/mL) was comparable to that of the reference drug, diclofenac sodium (EC50=0,57±0,21µg/mL). To evaluate the hepatoprotective activity of the methanol extract of Trang To leaf, the liver damage was induced in mice given with carbon tetrachloride (CCl4) mixed in olive oil (1: 4) at a dose of 2.5 mL/kg body weight/day. As a control, the standard hepatoprotective agent, silymarin, was used at the dose of 16 mg/kg body weight for 4 consecutive weeks. Trang To leaf extract was given at three different doses 100, 200 and 400 mg/kg body weight. The results showed the effective dose-dependent reduction of transaminase enzyme (ALT and AST) levels in sera. In addition, Trang To leaf extract also improved the oxidative stress status of the liver through effective reduction of MDA level and increase of GSH level in the liver. Histopathological examination of the liver tissue revealed that the mice treated with Trang To leaf extract showed significant improvement of liver tissue damages similar to silymarin treatment compared to the non-treated control group. The present results demonstrated that Trang To leaf extract has potent antioxidant and hepatoprotective activity. ","PeriodicalId":409863,"journal":{"name":"TAP CHI SINH HOC","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131395787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Evaluating cytotoxic effect of the extracted compounds from Ehretia asperula Zoll. & Mor stem on several cancer cell lines 曲霉Ehretia asperula Zoll提取化合物的细胞毒作用评价。&更多的干细胞在几种癌细胞系上

TAP CHI SINH HOC Pub Date : 2018-11-06 DOI: 10.15625/0866-7160/V40N2.12955

Vu Thi Nguyet, N. Dat, L. Huong, Tran Thị Hong Ha, Nguyen Hong Chuyen, N. Hang, Dang Dinh Kim

{"title":"Evaluating cytotoxic effect of the extracted compounds from Ehretia asperula Zoll. & Mor stem on several cancer cell lines","authors":"Vu Thi Nguyet, N. Dat, L. Huong, Tran Thị Hong Ha, Nguyen Hong Chuyen, N. Hang, Dang Dinh Kim","doi":"10.15625/0866-7160/V40N2.12955","DOIUrl":"https://doi.org/10.15625/0866-7160/V40N2.12955","url":null,"abstract":"This paper reports the cytotoxic effect on several cancer cell lines of the stem extracts and of some isolated compounds from Ehretia asperula. All the extracts exhibited cytotoxic effects on at least one cancer cell line. The n-hexane extract showed potent cytotoxic activity on Hep-G2, MCF-7 and HeLa cell lines with IC50 values of 28.3 g/ml, 14.42 g/ml and 18.59 g/ml, respectively, while the methanolic, ethyl acetate and water extracts exhibited toxicity towards MCF-7 cells with IC50 values of 16.45 g/ml, 13.4 g/ml and \u000039.78 g/ml, respectively. \u000006 compounds have been isolated from the ethyl acetate fraction of Ehretia asperula stem. Methyl caffeate has a strong cytotoxicity against Hep-G2, HeLa and MCF-7 cancer cell lines with IC50 values of 2.83 g/ml, 3.38 g/ml and 4.4 g/ml, respectively. Oresbiusin B was active against Hep-G2 with IC50 value of 9.89 g/ml. The other compounds including coniferaldehyde, 9′-methoxydehydrodiconiferyl alcohol and vanillic acid did not have any cytotoxic effect on the tested cancer cell lines. So, the obtained results have suggested possibility of using the potential Ehretia asperula extracts as health food for preventing and curing cancer diseases. \u0000Keywords: Ehretia asperula, methyl caffeate, oresbiusin B, Cancer cell lines. \u0000Citation: Vu Thi Nguyet, Nguyen Tien Đat, Le Mai Huong, Tran Thi Hong Ha, Nguyen Hong Chuyen, Nguyen Thi Hang4, Đang Đinh Kim, 2018. Evaluating cytotoxic effect of the extracted compounds from ehretia asperula zoll. & mor stem on several cancer cell lines.Tap chi Sinh hoc, 40(2): 145153. https://doi.org/10.15625/0866-7160/v40n2.12955. \u0000*Corresponding author: dangkim.iet@gmail.com","PeriodicalId":409863,"journal":{"name":"TAP CHI SINH HOC","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126582769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1