TAP CHI SINH HOC最新文献

{"title":"H(_{2})O(_{2}) production in Lactobacillus strains isolated from the intestinal microbiome of healthy people","authors":"H. Thu, T. Thạch, N. Hoa, L. L. T. Anh, V. Hien, N. Duy, D. Quyen, N. Nhung","doi":"10.15625/0866-7160/v42n1.14508","DOIUrl":"https://doi.org/10.15625/0866-7160/v42n1.14508","url":null,"abstract":"Lactobacillus sp. in the digestive tract are capable of producing H2O2 to inhibit the growth of harmful bacteria and balance the gut microflora. In this study, we have isolated 115 strains of Lactobacillus spp. from stool samples of healthy people in Ha Noi. Of the 50 tested Lactobacillus strains, 9 strains were capable of producing H2O2, of which the Lac.VFE-14 strain produced highly H2O2 with a concentration of 2.183 mM, followed by Lac.VFE-08 strains (2.081 mM) and Lac.VFE-04 (2.067 mM). All three strains grew well in MRS medium supplemented with bile salts or adjusted to low pH value. With 0.3% of bile salt, the survival rates of these 3 strains were 99%, 95% and 97%, respectively. At pH 3.0, after 3 hours of cultivation, the survival rates of the three strains were 98.54%, 94.15% and 95.27%, respectively. In addition, each of the cell-free culture supernatants of these three strains that inhibit the growth of S. aureus ATCC-23235. The inhibition zone diameters of the three strains were 19.0±1.0 mm, 14.0±1.0 mm and 11.7±1.3 mm, respectively. The results of 16S rRNA gene analyses showed that Lac.VFE-14, Lac.VFE-08 and Lac.VFE-04 had high similarity scores with L. plantarum ZZU 23 (100%), L. rhamnosus JCM 1136 (99%) and L. plantarum S7 (98.65%), respectively. This study indicates that all three strains have the potential to be used as probiotics in the future.  ","PeriodicalId":409863,"journal":{"name":"TAP CHI SINH HOC","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122863578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selection of purification condition for recombinant endoglucanase originated from goat rumen bacteria in Escherichia coli","authors":"N. Quy, N. Dương, Dao Trong Khoa, N. Việt, Nguyen Khanh Hai, T. N. Hải, Do Thi Thanh Huyen","doi":"10.15625/0866-7160/v42n1.14455","DOIUrl":"https://doi.org/10.15625/0866-7160/v42n1.14455","url":null,"abstract":"Cellulase is an important enzyme that plays a role in cleaving β-1,4 glucoside on cellulose to release glucose, which is of economic value and can be applied in many different fields. The 1545 bp endoglucanase gene mined from goat rumen's bacterial metagenomic data was expressed in Escherichia coli Rosetta2. In this study, the recombinant endoglucanase was purified by his-tag affinity chromatography with differrent processes, such as using phosphate buffer with or without sodium cloride, pretreatment of samples with ammonium sulphate before supplying into affinity column, using various concentration of imidazole for washing... Finally the endoglucanse was sucessfully purified by his-tag affinity column using sodium chloride-free phosphate buffer of which 150 mM and 400 mM imidazole were used for washing and enzyme elution, respectively. The resulting enzyme showed its high purity of 99%. CMC plate assay confirmed that although less active than commercial cellulase (Sigma), the recombinant cellulase hydrolyzed CMC to form a clear zone (halo) around the well. The purified enzyme is capable of using as material for further analysis. ","PeriodicalId":409863,"journal":{"name":"TAP CHI SINH HOC","volume":"6 Suppl 1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115069982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Provitamin a biofortification in maize through genetic engineering and marker-assisted selection","authors":"N. D. Thanh","doi":"10.15625/0866-7160/v41n4.13804","DOIUrl":"https://doi.org/10.15625/0866-7160/v41n4.13804","url":null,"abstract":"Maize is an important cereal in the global economy, which feeds one-third of the world’s population and is the third largest food crop after wheat and rice. Nutritional quality of most maize varieties is very low due to the lack of lysine and tryptophan and extremely low provitamin A carotenoids including α-carotene, β-carotene, and β-cryptoxanthin. Therefore, one of the solutions to improve nutritional value in maize is to improve provitamin A carotenoids contents. Many efforts have been made to produce maize plants with enhanced provitamin A carotenoids, especially, β -carotene. This article aims to review the research towards provitamin A biofortification through genetic engineering and marker-assisted selection. The published results and our recent achievements open the window for the improvement of provitamin A carotenoids in maize as well as the issues that need the further investigation. ","PeriodicalId":409863,"journal":{"name":"TAP CHI SINH HOC","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130149724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First report on two ostracod genera Loxoconcha Sars, 1866 (Loxoconchidae) and Xestoleberis Sars, 1866 (Xestoleberididae) along the coast of Vietam","authors":"Le Doan Dung, Akira Tsukagoshi","doi":"10.15625/0866-7160/v41n4.13972","DOIUrl":"https://doi.org/10.15625/0866-7160/v41n4.13972","url":null,"abstract":"One survey was done with SCUBA diving in Ha Long Bay and Cat Ba Island, Northern Vietnam (December 2013) and another one at Nha Trang Bay Marine Protected Area, Nha Trang city, Central Vietnam and Phu Quoc Marine Protected Area, Kien Giang Province, Southern Vietnam (November 2014). Sixteen species of the genus Loxoconcha and fourteen species of the genus Xestoleberis were found and identified. Eight species of the genus Loxoconcha were identified in group A, three species in group B, two in group C and three unidentified. Meanwhile, twelve species of the genus Xestoleberis were classified in group A and two in group B according to phylogenetic groups. From geographical distribution of the pore groups of the species in two genera Loxoconcha and Xestoleberis showed that the fauna of these genera in Vietnam is close to those of southern part of Japanese Island Arc, i.e., from Amami Island to the Philippines and Australia rather than Japanese Island Arc faunas.  ","PeriodicalId":409863,"journal":{"name":"TAP CHI SINH HOC","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130909060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and identification of indole acetic acid producing bacteria from the coasts of Ben Tre and Tra Vinh Provinces","authors":"Nguyen Ngoc Lan, V. Dzung, N. T. Lien, Nguyen Thi Kim Thoa, D. H. Nghi, N. Hoang","doi":"10.15625/0866-7160/v41n4.13869","DOIUrl":"https://doi.org/10.15625/0866-7160/v41n4.13869","url":null,"abstract":"Beneficial plant-growth-promoting bacteria (PGPB) have been reasonably applied to rescue crucial issue for agriculture by salinity soil. Observed most of PGPB was found in endophyte, rhizosphere and soil. Indole acetic acid (IAA)-producing bacteria could naturally stimulate and facilitate plant growth. The knowledge of IAA production and content of bacteria resident in the marine environment has been typically insufficient and limited to date. In recent years, unwarrantable intrusions of sea water have been enlarged in the Mekong River Delta of Vietnam, threatening productive rice fields, local fruits, and cash crops. Therefore, finding PGPB in the coastal regions in the Mekong River Delta as a creative resource for sustainable agriculture is necessary and is a prompt challenge. In this study, IAA-producing bacteria from coastal regions of Ben Tre and Tra Vinh Provinces were isolated and adequately identified. Out of 202 bacterial isolates, 10 isolates showed the possible ability to produce IAA from L-tryptophan. These 10 isolates were objectively evaluated the capacity to produce IAA under 5% (w/v) NaCl in King B and marine broths. The results revealed that IAA production decreased in 5% NaCl, even though bacterial growth increased. These 10 IAA-producing bacteria were classified at the species level, Marinobacter hydrocarbonoclasticus, M. pelagius, M. daepoensis, and Mameliella phaeodactyli by 16S rRNA gene analysis. The most IAA producer in King’s B broth, the isolate C7, was investigated in more detail. The isolate C7 produced the maximum IAA amount (192.2 ± 1.14 µg/ml) under the presence of 20 g/l yeast extract, 2 g/l of L-tryptophan and 1% NaCl. The isolate C7 was able to grow at 1–17% (w/v) NaCl (optimum, 4%), but not in the absence of NaCl, indicating it is a moderate halophilic bacteria. This study highlighted the considerable ability to produce IAA of marine bacteria, which could be thoughtfully considered to use naturally as biofertilizers to promote plant growth in saline intrusion lands. ","PeriodicalId":409863,"journal":{"name":"TAP CHI SINH HOC","volume":"81 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124887397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New records and morphological assessments of long-nosed fruit bats (chiroptera: pteropodidae: Macroglossus spp.) from Vietnam","authors":"Hoang T. P. Thanh, N. T. Son, V. T. Duong, Nguyễn Thành Lương, Dao Nhan Loi, V. D. Thong","doi":"10.15625/0866-7160/v41n4.14695","DOIUrl":"https://doi.org/10.15625/0866-7160/v41n4.14695","url":null,"abstract":"Long-nosed fruit bat is a common name of the genus Macroglossus which comprises two species: Dagger-toothed long-nosed fruit bat (M. minimus) and Greater long-nosed fruit bat (M. sobrinus). These two species were rarely recorded from Vietnam or neighboring countries. Within Vietnam, M. minimus has been recorded only from two localities in southern Vietnam while M. sobrinus was known from all northern, central and southern regions of the country. Morphological features of these species in Vietnam were poorly documented in previous publications. With results from a rapid examination of all specimens and recently captured individuals, we here confirm that M. sobrinus is distinctively larger than M. minimus in all external and craniodental measurements. Two species are also distinguishable by their nostril shapes and mandible symphyses. This paper provides new distributional records of both M. sobrinus and M. minimus from Vietnam with remarks on their ecology and habitats. ","PeriodicalId":409863,"journal":{"name":"TAP CHI SINH HOC","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127737651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First record of Anselmella malacia Xiao & Huang, 2006 (Hymenoptera: Eulophidae), a new insect pest of water apple (Syzygium samarangense) and its associated parasitoid in Dong Thap Province, Vietnam","authors":"N. Oanh, Nguyen Kim Bup, Khuat Dang Long","doi":"10.15625/0866-7160/v41n4.14673","DOIUrl":"https://doi.org/10.15625/0866-7160/v41n4.14673","url":null,"abstract":" The eulophid wasp, Anselmella malacia Xiao & Huang, 2006 (Hymenoptera: Eulophidae), is reared from fruits of the water apple, Syzygium samarangense, and is reported as an important phytophagous developing as seed-eaters of Syzygium species in Malaysia. Analysing specimens of pest insects infested inside of pear-shaped fruits of the cultivated water apple, S. samarangense, in Dong Thap Province, South Vietnam, a total of four insect species were recorded as the important pests for fruits of six varieties of the cultivated water apples. The hymenoteran wasp, A. malacia, is recorded for the first time from Vietnam. Further evidence is needed to prove A. malacia being an invasive pest in Vietnam. The associated hymenopteran parasitoid assemblage with the fruit infested insect pests is also provided. ","PeriodicalId":409863,"journal":{"name":"TAP CHI SINH HOC","volume":"387 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122095312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A new species of the genus Trigonura Sichel, 1865 (Hymenoptera: Chalcididae) from Vietnam","authors":"Khuat Dang Long, Nguyen Duc Hiep, D. Hoa, Tran Thi Ngat","doi":"10.15625/0866-7160/v41n4.14030","DOIUrl":"https://doi.org/10.15625/0866-7160/v41n4.14030","url":null,"abstract":"Based on the specimens of chalcid parasitoid wasps collected by malaise traps set in fruit orchards in the adjacent area of Ha Noi city from 2018–2019, one new species of the genus Trigonura, T.  vietnamica Long, sp. n., was described and illustrated. Two species of this genus from Vietnam are keyed, the comparative characters of the new species were also given to compare with two similar species from the Oriental Region, T. indica Narendran and T. luzonensis Narendran.  ","PeriodicalId":409863,"journal":{"name":"TAP CHI SINH HOC","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123132730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biological characteristics of the japanese scad Decapterus maruadsi in Thuan An estuary, Thua Thien Hue Province","authors":"Tran Thi Minh Anh, N. Huấn, T. Thanh, Nguyen Thanh Nam","doi":"10.15625/0866-7160/v41n4.13891","DOIUrl":"https://doi.org/10.15625/0866-7160/v41n4.13891","url":null,"abstract":"A total of 257 Decapterus maruadsi individuals of collected from Thuan An estuary in Phu Vang District, Thua Thien Hue Province were analyzed. Results showed that the fork length ranged from 90 mm to 215 mm (160.2 mm on average) and mainly in the 140–180 mm range (86.8 %). Of three age groups (0+ to 2+), the fished stock belongfrom is primarily to the 1+ age group (63.8%) and 0+ age group (28.8%). Of five stomach fullness levels (0 to 4), the second and fourth degree made up significantly proportions, being 29.6% and 31.5%, respectively. For the five fat levels (0 to 4), the fourth degree made up 86.0%.  The females to males ratio was 1.00 : 1.42.  von Bertalanffy’s growth model followed the equation: Lt = 203.77 × (1–e-0.3747(t+3.0272)).  ","PeriodicalId":409863,"journal":{"name":"TAP CHI SINH HOC","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131482003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A checklist of pteridophytes collected on cham chu mountainsand adjacent sites","authors":"P. K. Lộc, V. T. Duong, Vu Cong Toan, Trinh Ngoc Bon","doi":"10.15625/0866-7160/v41n4.14639","DOIUrl":"https://doi.org/10.15625/0866-7160/v41n4.14639","url":null,"abstract":"Located in Tuyen Quang and Ha Giang Provinces, the Cham Chu Nature reserve (NR) has diverse plant ecosystem types, and its the flora is rich in species. However,  the pteridophyte flora of Tuyen Quang and Ha Giang Provinces has not been so far generallystudied. This study presented results of three biotic surveys conducted on Cham Chu mountainsand adjacent sites surveysin 2018−2019. A total of 123 plant specimens gathered and analyzed. with 84 plant species of 43 genera belonging to  23 families recorded. The family Polypodiaceae is therichestwith 8 genera and 12 species. Twogenera, Tectaria and and Pteris, are richin species, with 9 and 8 species, respectively. A checklist of  species was also provided. ","PeriodicalId":409863,"journal":{"name":"TAP CHI SINH HOC","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130957214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}