TAP CHI SINH HOC最新文献_第4页

Screening genes encoding protein protease inhibitor from metagenome of sponge-associated microorganisms in Quang Tri sea, Vietnam 从越南广治海海绵体相关微生物宏基因组中筛选蛋白蛋白酶抑制剂基因

TAP CHI SINH HOC Pub Date : 2019-06-11 DOI: 10.15625/0866-7160/V41N2.13683

T. Hong, P. V. Cuong, N. Cuc

{"title":"Screening genes encoding protein protease inhibitor from metagenome of sponge-associated microorganisms in Quang Tri sea, Vietnam","authors":"T. Hong, P. V. Cuong, N. Cuc","doi":"10.15625/0866-7160/V41N2.13683","DOIUrl":"https://doi.org/10.15625/0866-7160/V41N2.13683","url":null,"abstract":"Using metagenomics-based method to isolate new compounds from the marine environment are getting more and more attention in recent years. Based on metagenome library, bioinformatics methods is a powerful tool for screening genes with new biological activities from uncultured microorganisms and become a breakthrough in research and application of biotechnology. In this study we selected and used the samples DNA QT2 which had high DNA content and purity from a total of 6 DNA samples of sponge-associated microorganisms collected in Quang Tri sea (Vietnam) for metagenomic sequencing (DNA concentration is 202.5 ng, A260/ A280 value is 1.80). 16S rRNA metagenomic sequencing data of QT2 produced 44,117,722 reads, which were assembled into 120,236 contigs. ORF prediction using Prodigal produced 386,416 ORFs. Functional annotation was conducted based on 7 different databases (NR, COG, CAZy, Swissprot, GO, KEGG, Pfam), and there are 266,553 genes were annotated using Swiss-Prot. In addition, based on the obtained metagenomic data, 50 complete genes encoding protease inhibitor proteins were revealed and among them, 28 genes encoding protein (> 50%) belonged to the serine protease inhibitor family, and 22 genes genes encoding belonged to the Inter-alpha-trypsin inhibitor group. NCBI BLAST screening results that these proteins had higher 50% identity to protease inhibitors. ","PeriodicalId":409863,"journal":{"name":"TAP CHI SINH HOC","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125455201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Formulation and application of nanosilver-infused cream in prevention and treatment of mastitis in dairy cows 纳米银乳膏预防和治疗奶牛乳腺炎的配方及应用

TAP CHI SINH HOC Pub Date : 2019-06-11 DOI: 10.15625/0866-7160/V41N2.13707

N. Chau, Dao Trong Hien, N. Thuy, Doan Quang Ha, D. Duong, T. T. Loan, Phung Thi Dieu Linh, T. Luu, N. D. Tan, T. V. Tựa

引用次数: 2

Utilization of Schizochytrium mangrovei PQ6 as feed for rotifer Brachionus plicatilis in rearing black sleeper’s larvae (Bostrichthys sinensis, Lacepede, 1881) mangrovei Schizochytrium PQ6作为粘连臂轮虫(Brachionus plicplatilis)饲料饲养黑睡虫幼虫的研究(Bostrichthys sinensis, Lacepede, 1881)

TAP CHI SINH HOC Pub Date : 2019-06-11 DOI: 10.15625/0866-7160/V41N2.13646

Phạm Thành Công, H. L. Anh, D. D. Hong

{"title":"Utilization of Schizochytrium mangrovei PQ6 as feed for rotifer Brachionus plicatilis in rearing black sleeper’s larvae (Bostrichthys sinensis, Lacepede, 1881)","authors":"Phạm Thành Công, H. L. Anh, D. D. Hong","doi":"10.15625/0866-7160/V41N2.13646","DOIUrl":"https://doi.org/10.15625/0866-7160/V41N2.13646","url":null,"abstract":" Schizochytrium mangrovei PQ6 contains many important polyunsaturated fatty acids, such as docosahexaenoic acid (DHA, C22: 6w-3), eicosahexaenoic acid (EPA, C20: 5w-3) and docosapentaenoic acid (DPA, C22: 5w-6). These fatty acids are essential for survival and growth of many marine fish larvae. In this paper, fresh biomass of S. mangrovei PQ6 was used for culturing rotifer Brachionus plicatilis. In the first formula (L1), rotifers were fed on baker’s yeast; in second formula (L2), rotifers were fed on mixed two microalgae, Nannochloropsis oculata and Chaetoceros gracilis; in the third formula (L3), rotifers were fed on S. mangrovei PQ6 biomass. The results indicated that S. mangrovei PQ6 biomass can replace baker’s yeast and autotrophic microalgae in feeding rotifers. Total lipid, total fatty acid and polyunsaturated fatty acid contents of rotifers fed on formula 3 (S. mangrovei PQ6) were higher than those within others. High polyunsaturated fatty acid (C ≥ 20) in omega-3 and 6 groups; DHA and DPA contents of rotifers fed on S. mangrovei PQ6 were the highest, accounting for 39.81% of total fatty acid; 41.95% and 8.24% polyunsaturated fatty acids, corresponding to 20.52; 12.15 and 2.4 mg/g of dried weight, respectively.The survival rate of black sleeper’s larvae was highest when they were fed on rotifers grown on L3 formula (51.20 ± 0.89%), followed by L2 formula (48.70 ± 2.67%) and the lowest with L1 formula (43.44 ± 1.54%) (P < 0.05). However, among three formulas, no significant difference was found in the growth of black sleeper’s larvae (P > 0.05). These results suggest that biomass of S. mangrovei PQ6 can replace traditional feed like autotrophic microalgae or baker’s yeast for biomass culture of the Brachionus plicatilis rotifer for rearing black sleeper’s larvae to enhance seed quality to meet demands of market. ","PeriodicalId":409863,"journal":{"name":"TAP CHI SINH HOC","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130225460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Three new species of the spiral nematode genus helicotylenchus steiner, 1945 (nematoda: hoplolaimidae) from Vietnam 标题越南螺旋线虫属三新种，1945(线虫纲:螺旋线虫科)

TAP CHI SINH HOC Pub Date : 2019-06-11 DOI: 10.15625/0866-7160/V41N2.13597

N. Châu, D. T. Anh

{"title":"Three new species of the spiral nematode genus helicotylenchus steiner, 1945 (nematoda: hoplolaimidae) from Vietnam","authors":"N. Châu, D. T. Anh","doi":"10.15625/0866-7160/V41N2.13597","DOIUrl":"https://doi.org/10.15625/0866-7160/V41N2.13597","url":null,"abstract":"Investigations on entomopathogenic and plant parasitic nematodes in some natural forests in the Ngoc Linh mountain (Quang Nam province) and the Thuong Xuan forest (Thanh Hoa province) revealed three new species of plant parasitic nematodes belonging to the genus Helicotylenchus Steiner, 1945 (Nematoda: Hoplolaimidae). These new species were named Helicotylenchus castanus sp. n., Helicotylenchus madhucus sp. n. and Helicotylenchus digitus sp. n. with morphological characteristics described and illustrated. The new species Helicotylenchus castanus sp.n. is characterized by the highly hemispherical shape of the lip region, extremely short and rounded tail without projection. The second new species Helicotylenchus madhucus sp. n. is characterized by high lip region with 4-5 annuli, hemispherical and not set off from body contour, stylet 31.3-35.9 (33.3 ± 1.3) µm long, tail with a short ventral projection and the presence of males in the population. The third new species, Helicotylenchus digitus sp.n. was recognized by broad rounded tail with strong annulated projection in the digital shape, and also the presence of males in population. ","PeriodicalId":409863,"journal":{"name":"TAP CHI SINH HOC","volume":"40 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125677998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

The impact of zerovalent cobalt nanoparticle on photosynthesis - related genes expression in soybean leaves Glycine max (L.) Merr. (Dt26) 零价钴纳米颗粒对大豆叶片光合相关基因表达的影响稳定。(Dt26)

TAP CHI SINH HOC Pub Date : 2019-03-28 DOI: 10.15625/0866-7160/V41N1.13540

L. T. Tam, H. M. Hien, H. L. Anh, Phan Hoàng Tuấn, D. D. Hong

{"title":"The impact of zerovalent cobalt nanoparticle on photosynthesis - related genes expression in soybean leaves Glycine max (L.) Merr. (Dt26)","authors":"L. T. Tam, H. M. Hien, H. L. Anh, Phan Hoàng Tuấn, D. D. Hong","doi":"10.15625/0866-7160/V41N1.13540","DOIUrl":"https://doi.org/10.15625/0866-7160/V41N1.13540","url":null,"abstract":"Photosynthesis is an important physiological process in plants. It plays a crucial role in plant growth and development. In this study, we investigated the impact of zerovalent cobalt nanoparticles on the photosynthesis and expressing of gene involving in this process in leave of soybean Glycine max (L.) Merr “DT26” at different growth stages. The results showed that treatment of zerovalent cobalt nanoparticles made in Vietnam and USA (with two doses of 0.17 and 16.67 mg/kg of soybean seed) enhanced the photosynthesis of soybean by increasing the content of chlorophyll a and the ratio of Fv/Fm compared with the control (without treatment of zerovalent cobalt nanoparticles). These values tended to increase and reached the maximum value at 40 days and then decreased in at 70 days. The expression level of photosynthesis-related genes of soybean leaves also changed depending on the soybean‟s growth stage and concentration of zerovalent cobalt nanoparticles being treated. The genes psaA, Lhca, psaB, Cytb6f (belonging to photosystem I) and psbA, psbB, psbC, psbD, psbE (belonging to photosystem II) in the experimental fomulas were higher expressed than that in control group at 20 and 70 days. However, at 40 days, the expression levels of these genes were significantly different. Obtained results supplied the basis for understanding the active mechanism of the above genes to control/regulates photosynthetic activity of plants with and without the presence of zerovalent cobalt nanoparticles as well as under stress conditions.","PeriodicalId":409863,"journal":{"name":"TAP CHI SINH HOC","volume":"138 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127014783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation of apoptosis of dendritic cells by il-10 and in association with stat-1 signaling molecule il-10对树突状细胞凋亡的调控及其与stat-1信号分子的关系

TAP CHI SINH HOC Pub Date : 2019-03-28 DOI: 10.15625/0866-7160/V41N1.12607

Nguyen Thi Thu Thuy, N. Xuan

{"title":"Regulation of apoptosis of dendritic cells by il-10 and in association with stat-1 signaling molecule","authors":"Nguyen Thi Thu Thuy, N. Xuan","doi":"10.15625/0866-7160/V41N1.12607","DOIUrl":"https://doi.org/10.15625/0866-7160/V41N1.12607","url":null,"abstract":"IL-10 is an anti-inflammatory cytokine, participating in induction of immune tolerance and cell apoptotic death. Dendritic cells (DCs) is the most professional antigen-presenting cells among innate immune cells to exert generation and maintenance of immunological memory mediated through activation of T and B lymphocytes. The STAT signalling pathway plays a regulatory role of maturation and differentiation of immune cells. In this study, DCs were treated with inflammatory cytokines including TNF-a, INFg, IL-2 and IL-10 and subsequently examined the phosphorylation of STAT-1 and STAT-3, TNF-α concetration in cell suspension and the proportion of Annexin V+ and caspase 3+ cells. Methods used for this investigation include western blotting, flow cytometry and ELISA. DCs were derived from mouse bone marrow cells and cultured with GM-CSF for 8 days. As a result, IL-10, but not other cytokines enhanced the number of Annexin V+cells and caspase 3 activity in DCs. More importantly, IL-10 also increased the phosphorylation of STAT-1 as well as the release of TNF-α into cell suspension. In conclusion, activation of STAT-1 might relate to the cell apoptotic death and TNF-α sectetion in IL-10-treated DCs.","PeriodicalId":409863,"journal":{"name":"TAP CHI SINH HOC","volume":"64 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130846252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Floristic composition of different habitats of indochinese silvered langur (Trachypithecus germaini) in the Chua Hang karst in Kien Luong district, Kien Giang province, Vietnam

TAP CHI SINH HOC Pub Date : 2019-03-28 DOI: 10.15625/0866-7160/V41N1.9740

Le Hong Thia, Hoang Minh Duc, H. Covert

{"title":"Floristic composition of different habitats of indochinese silvered langur (Trachypithecus germaini) in the Chua Hang karst in Kien Luong district, Kien Giang province, Vietnam","authors":"Le Hong Thia, Hoang Minh Duc, H. Covert","doi":"10.15625/0866-7160/V41N1.9740","DOIUrl":"https://doi.org/10.15625/0866-7160/V41N1.9740","url":null,"abstract":"The Indochinese silvered langur (Trachypithecus germaini) is a leaf monkey species that occurs on four karst hills in the Kien Luong District, Kien Giang Province, Vietnam. In this study, the floristic composition of four habitats where the silvered langur were observed feeding was analyzed. These habitats include cliffs, slopes, the peak of the Chua Hang karst and the adjacent mangroves. We identified 185 plant species belonging to 61 families distributing across the habitats, and the silvered langurs fed on 60 plant species of 37 families. Among food plants selected by the silvered langur, 41 plant species were distributed on the cliff, followed by the slope (31 species), the peak (24 species), and the mangroves (4 species). The silvered langurs fed on about 67% of plant species on the cliff. Plant species richness of the slope areas was highest (at D=0.45, D: Simpson index, and d=20.0, d: Margalef index) compared with the other habitats. The floristic composition of the cliff, slope and peak was quite similar to each other, with the similarity index (SI) ranging from 0.47 to 0.56. The results showed that four plant species, Phyllathus reticulatus Poir, Ficus rumphii Blume, Streblus ilicifolia (Kurz.) Corn. and Cayratia trifolia L. were consumed throughout of the year. In summary, present results add important information for conservation of the Indochinese silvered langur in Vietnam. ","PeriodicalId":409863,"journal":{"name":"TAP CHI SINH HOC","volume":"37 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124909794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Some new recordeds of macrofungi from the Ngoc Linh mountain, Quang Nam province, Vietnam 标题越南广南省玉林山大型真菌新记录

TAP CHI SINH HOC Pub Date : 2019-03-28 DOI: 10.15625/0866-7160/V41N1.12937

T. Phu, T. T. Kiet

引用次数: 0

A nutrient method for cutivation of macroalgae Ulva papenfussii 一种培养大型藻叶藻的营养法

TAP CHI SINH HOC Pub Date : 2019-03-28 DOI: 10.15625/0866-7160/V41N1.12590

Vo T. Trung, T. Huynh, Tran Mai Duc, Phan Duc Thinh, P. T. San, Nguyễn Thị Thanh Hằng

引用次数: 0

Diversity of bats (Mammalia: Chiroptera) from the sop cop special use forest, son la province, Vietnam 越南圣罗省索普普特殊用途森林蝙蝠的多样性(哺乳目:翼翅目)

TAP CHI SINH HOC Pub Date : 2019-03-28 DOI: 10.15625/0866-7160/V41N1.13327

Dao Nhan Loi

引用次数: 0