RAS Microbiology and Infectious Diseases最新文献

{"title":"The Dual Energetic State of Living Organisms: Life. death and restitution","authors":"B. Scherlag, Ronald A. Scherlag, Tarun W. Dasari, Sunny S. Po","doi":"10.51520/2766-838x-23","DOIUrl":"https://doi.org/10.51520/2766-838x-23","url":null,"abstract":"Our initial study demonstrated the release of a replicate cell from a dwarf form of Stentor coeurleus that had undergone apoptosis, i.e., programmed cell death. The replicate separated from the moribund cell and faded into the ambient environment. In the present series of experiments actively mobile, transparent, dwarf cells and algae fragments were returned from the ambient state into sealed deep well slides containing cell free protozoa medium. Ancillary experiments provided evidence that these cells displayed electromagnetic energy. Further observation over several days showed a progressive transformation of the dwarf cell by accruing inclusions to a senescent state and eventual encysted state. We hypothesize that these unicellular cells and algae fragment consisted of metabolic and electromagnetic energetic forms that can be separated at the life/death interface and returned from the ambient environment by electromagnetic energy. Restitution of the metabolic form and senescent is the final process.","PeriodicalId":409797,"journal":{"name":"RAS Microbiology and Infectious Diseases","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129955056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid diagnosis of acute respiratory infections by multiplex endpoint PCR technology","authors":"A. Udristioiu, M. Cojocaru, Dana Alexandra Maria Panait, Nica-Badea Delia","doi":"10.51520/2766-838x-13","DOIUrl":"https://doi.org/10.51520/2766-838x-13","url":null,"abstract":"Introduction: The multiplex endpoint PCR technology offers a number of potential advantages, results are available in a matter of hours rather than days, the extreme sensibility facilitates detection of even minutes the amounts of pathogen DNA in clinical samples and the test is not significantly affected by prior administration of antibiotics.\u0000Aim: The aim of this work was to rapidly identify the antibiotic resistance the monitoring of pathogen growth at the patients admitted in Hospitalization Intensive Care Unit, with the diagnosis of Community Acquired Pneumonia, (CAP).\u0000Method: The Analyzer Unyvero™ Pneumonia Application was used in detection of pneumonia associated pathogens and their antibiotic resistance genes using the Pneumonia Unyvero™ System, following PCR pathogen species with sequencing of the amplified microbial DNA.\u0000Results: The main pathogens of community acquired pneumonia from the cohort study,36 cases, (20 males in mean age 35-66 years and 16 females in mean age 40-55 mean years), were Streptococcus pneumonia, (16 cases), Staphilococcus aureus, (10 cases), Klebsiella pneumonia (5 cases) and other important agents were “atypical”, such as Haemophilus Influenzae, Chlamidophilapneumonie and Moraxelacataralis. A case with Acinetobacter baumani and Proteus Sp. was also widely resistance to mefA gene / ermB gene as all cases of analyzed. The more frequency of genes resistant (29 cases) are ermA gene / ermC / ermB for Staphilococcus aureus and the gene tem+shv / gene / ctx-M with the Chromosomal mutation (7 cases), as gyrA83_87 Ecoli / Pseu for Klebsiella pneumonia agents. Also, most resistance antibiotics were Makrolides, (29 cases and Lincosamides (6 cases) and these cases have had the chromosomial integrates. The most resistance microbe, Pseudomonas aeruginosa (1 case), has been registered as multi drugs resistance [MDR]*.\u0000Conclusion: The Unyvero™ results have been available 2 days before the primary microbiology report and 3 days before the final confirmation results, obtained by microbiology culture. The Unyvero Analyzer only provides rapid data to support the therapeutic decision of currant medic.","PeriodicalId":409797,"journal":{"name":"RAS Microbiology and Infectious Diseases","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122252356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Contamination of hospital environment with multi drug resistant (MDR) bacteria responsible for healthcare acquired infections: Case of Universitary Hospital Center of Marrakesh-Morocco","authors":"A. Hilmi, L. A. Said, A. Ouafi, L. Azlay, E. Oubassou, K. Tigui, K. Zahlane","doi":"10.51520/2766-838x-20","DOIUrl":"https://doi.org/10.51520/2766-838x-20","url":null,"abstract":"The hospital environment plays a significant role in the transmission of healthcare acquired multidrug resistant bacteria among hospitalized patients, especially in high-risk infectious units. Therefore, the present study aims to describe the distribution of microorganisms present on inanimate surfaces, hospital equipment and healthcare workers hands of the hospital units in Marrakech’s Mohammed VI University Hospital (UHC).","PeriodicalId":409797,"journal":{"name":"RAS Microbiology and Infectious Diseases","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115663303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of Negative Ions to Inactivate COVID-19 Coronaviruses and Variants","authors":"B. Scherlag, Ronald A. Scherlag, S. Po, Tarun W. Dasari","doi":"10.51520/2766-838x-21","DOIUrl":"https://doi.org/10.51520/2766-838x-21","url":null,"abstract":"The COVID-19 pandemic has taken an enormous toll of morbidity and mortality globally. After attempts with drug therapy failed, the implementation of extensive vaccination programs have been able to mitigate but not completely eliminate the pandemic threat. A major drawback that has prevented progress in combating the toll of infections, hospitalizations and deaths has been the emergence of mutations. In the present report we propose a new approach for the prevention of COVID-19 and its variants.","PeriodicalId":409797,"journal":{"name":"RAS Microbiology and Infectious Diseases","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128223412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantification of IgG specific antibodies in recovered COVID -19 patients and its association with disease severity","authors":"S. Srivastava, S. Bahadur, Shalini Shukla, R. Upadhyay, J. Sickan, Ajay Darbar, Hussain Ali, A. Beshiri","doi":"10.51520/2766-838x-14","DOIUrl":"https://doi.org/10.51520/2766-838x-14","url":null,"abstract":"Background: Serological tests for anti-SARS-CoV-2 antibodies can provide valuable information in epidemiologic and public health research. However, the data is limited on serological response post COVID-19 infection. Objective: To investigate the use of ARCHITECT SARS CoV-2 IgG II Quantitative assay to quantify the IgG antibodies in recovered COVID-19 infections.\u0000Methods: Abbott SARS-CoV-2 IgG assays were performed using the ARCHITECT Quantitative chemiluminescent microparticle immunoassay to detect IgG against SARS-CoV-2 S1 receptor binding domain (RBD) in serum and plasma. Patients having IgG concentrations above ≥50 AU/mL were defined as positive. We analyzed 333 COVID-19 patients who were tested negative by RT-PCR not more than three months after testing positive.\u0000Results: The mean age of the population was 34.3 years and 95% were male. About 93% of the patients had mild disease and rest had moderate disease. The mean IgG levels in the entire cohort was 1860.27 AU/mL, the lowest and highest value detected by ARCHITECT SARS CoV-2 IgG II Quantitative Assay (Abbott Laboratories, USA) were 39.8 AU/mL and 43657.4 AU/mL, respectively. While patients with moderate COVID-19 disease had numerically higher IgG levels than patients with mild disease, the difference was not statistically significant (1787.7 AU/mL vs. 2865.39 AU/mL, p=0.21). In 97% of the patients, IgG antibodies could be detected up to 95 days after testing negative on RT-PCR.\u0000Conclusion: Patients with moderate COVID-19 disease may develop higher IgG antibodies than in patients with mild disease. Antibodies can be detected in majority of recovered individuals for more than 90 days which may be valuable in developing future vaccination strategy.","PeriodicalId":409797,"journal":{"name":"RAS Microbiology and Infectious Diseases","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126444654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of methicillin resistant Staphylococcus aureus and risks factors in pig farmers in rural West region, Cameroon","authors":"Célestin Tagne Kamga, Gildas Tazemda, J. Magne, Michel Kengne, Suzie Lacmago, Pomte Olivier, Danielle Wonkam, Sonia Anouwou, I. Djuikoue","doi":"10.51520/2766-838x-19","DOIUrl":"https://doi.org/10.51520/2766-838x-19","url":null,"abstract":"Background: Animal farming can be a potential reservoir of microbial resistance. Little data and information are documented on the prevalence and spread of methicillin resistant Staphylococcus aureus (MRSA) in remote and rural and semi-urban settings in developing countries. Yet, the increasing spread of antibiotics resistance either in the community or at the hospital has become a major public health problem in Cameroon. This study aimed to determine the prevalence of nasal methicillin resistant Staphylococcus aureus carriers among pig farmers and breeders in the West Region of Cameroon.\u0000\u0000Methods: A total of 103 nasal scraping samples were collected in four distant division of Bamboutos, Nkoung-Khi, Menoua and Mifi for microbiological, biochemical and sensitivity testing according to the standard protocol of CASFM 2018.\u0000\u0000Results: A high presence of Staphylococcus aureus of 29.13% was documented. S. aureus resistant showed 93.3%, to the ß-lactamases family, with MRSA prevalence at 27.18%. Resistance to glycopeptides (vancomycin) was 60%, macrolides-lincosamides-streptogramines mainly resistance to erythromycin 90% and clindamycin 30.6%, tetracycline 83.3%, fusidic acid 96.6% and to aminosides (tobramycin and gentamycin 76.6%). All isolated samples were sensitive to fluoroquinolones, notably to ofloxacin (80%) and to ciprofloxacin (86.6%).\u0000\u0000Conclusion: There is an urgent need to implement a robust and sustainable MRSA and AMR surveillance in curbing the growing threat locally.","PeriodicalId":409797,"journal":{"name":"RAS Microbiology and Infectious Diseases","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122323721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aeromonas Species in Meat Samples Obtained From Some Markets in Parts of Plateau State, Nigeria","authors":"E. M., I. J. U, Onwuliri F.O., D. F.O.","doi":"10.51520/2766-838x-18","DOIUrl":"https://doi.org/10.51520/2766-838x-18","url":null,"abstract":"Aeromonas species are emerging food borne pathogens with serious potentials for causing food borne diseases. They are estuarine bacteria and are ubiquitous in fresh water, ground water, bottled water, meats and fresh vegetables. A total of 800 meat samples, comprising of 4 different meat types; Chicken (200), beef (200), Fish (200) and chevon (goat meat) (200) were collected in the period of one year (from June 2017—June 2018) in Jos, Miango, Bukuru, Vom and Lamingo areas of Plateau state. These meat samples were analyzed for the presence of Aeromonas species using Aeromonas Dextrin agar and MacConkey agar. Overall 114 meat samples out of the 800 samples analyzed were positive for Aeromonas species with the beef having the highest percentage prevalence of 9.25% while the chevon had the lowest percentage prevalence of 5.75%. The result of this study revealed the presence of potentially pathogenic Aeromonas species in the meat samples obtained from these locations.","PeriodicalId":409797,"journal":{"name":"RAS Microbiology and Infectious Diseases","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116107493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carbapenemase Producing Klebsiella species from Hospitalised Patients at Three Referral Hospitals in Yaounde; Cameroon","authors":"Emilia Enjema Lyonga Mbamyah, Mangum Patience Kumcho, T. Michel, Dieudonné Sedena, Florence Anjabie Enyeji, Aime Cesar Teukam Noupe, Modestine Djuissi, M. Mesembe, G. Ikomey, A. Eyoh, H. Gonsu","doi":"10.51520/2766-838x-17","DOIUrl":"https://doi.org/10.51520/2766-838x-17","url":null,"abstract":"Background: Carbapenemases are the most potent beta-lactamases capable of hydrolysing all beta-lactam drugs. Data scarcity on laboratory evidence of carbapenemase producers obstructs infection prevention and effective control measures. This study aimed to detect and phenotypically characterize Klebsiella species resistant to carbapenems.\u0000\u0000Method: A total of eighty-seven (87) Klebsiella spp. isolates were collected from three Yaounde-based tertiary hospitals over a period of twelve months (February 2019 to January 2020). The isolates were identified on API 20E and antimicrobial susceptibility testing was done according to the Kirby Bauer disc diffusion method. Carbapenem resistance was detected based on reduction in inhibition zone diameter of meropenem less than 28mm and resistance phenotypes determined by the combination disc synergy test.\u0000\u0000 Results: Four Klebsiella species were identified namely: Klebsiella pneumoniae 62/87 (71.3%), K. oxytoca 11/87 (12.6%), K. ozaenae 7/87 (8%), K. rhinoscleromatis 7/87 (8%). Klebsiella pneumoniae was most resistant to carbapenems with a considerably high number of multidrug resistant isolates 39/56 (69.6%). Patients from the 0-9 years age group were most represented 29/87 (33.3%). Carbapenemases belonging to Ambler classes A, B and D were detected as follows: KPC 2/87 (2.3%), MBL 2/87(2.3%), OXA-48 4/87 (4.6%). All four Klebsiella species were producers of KPC carbapenemase 12/87 (13.8%) and Extended Spectrum β-lactamase 68/87 (78.2%) enzymes.\u0000\u0000Conclusion: These results revealed that more than half of Klebsiella species analysed were multidrug resistant and about a quarter in hospitalized patients at the referral hospitals were mostly KPC carbapenemase producers. This raises concerns over the need to control and confine the spread of these isolates of clinical and epidemiological importance for effective infection prevention and control.","PeriodicalId":409797,"journal":{"name":"RAS Microbiology and Infectious Diseases","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130382488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibiotics Resistant Genes and Plasmid curing of Multidrug Resistant Clinical Bacterial Isolates from the Three Geographical Regions of Ondo State, Nigeria","authors":"Olajubu F.A., Ajayi A.o., Fadipe D.O., Yakubu P.","doi":"10.51520/2766-838x-15","DOIUrl":"https://doi.org/10.51520/2766-838x-15","url":null,"abstract":"This study shows the characteristics of multidrug resistant clinical isolates analyzed for their antibiotic resistant genes in laboratory. Genomic DNA was then extracted from each sample using Column-pure Bacteria Genomic DNA Isolation kit. Similarly, plasmid profile, plasmid curing and the antibiotic susceptibility profile of thirty two (32) cultured multidrug resistant clinical isolates were analyzed for their antibiotic resistant genes. The bacterial species tested are Acinetobacter haemolyticus, Budricia aquatic, Burkhoderia cepacia, Citrobacter gilleric, Enterobacter gergoriac,Enterobacter agglomerane, Escherichia coli, Escherichia hermamii, Klebsiella oxytoca, Klebsiella ornithinolytica, Klebsiella terrigena, Pseudomonas aeruginosa, Pseudomonas fluorescens, Staphylococcus spp., Staphylococcus aureus and coagulase –ve, Staphylococcus spp. Nutrient agar and Mueller Hinton Agar were used routinely during the study. The genomic test done on the thirty two (32) multidrug resistant strains did not show any resistant gene. But, the molecular plasmid profile conducted shows that all the isolates possess plasmid. Plasmid curing done reduces the antibiotics resistance nature of the isolates. This shows that the resistance of the isolates were plasmid mediated. The re-assessment of the antibiotic susceptibility profile of the clinical bacterial isolates tested confirmed this. It was also clarified in this study that most of the selected resistant bacterial strains isolated from hospitals were susceptible to medicinal plants such as Moringa oleifera and Andrographis peniculata tested on them. This implies that the use of appropriate medicinal plants can be optimized for pharmaceutical purpose to overcome infections caused by these aetiologic agents.","PeriodicalId":409797,"journal":{"name":"RAS Microbiology and Infectious Diseases","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126697379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of neutrofiles and by macrophages in the pathogenesis of inflammatory and I post inflammatory episodes : Major tissue damage may be inflicted by a synergy among their toxic pro inflammatory agents","authors":"I. Ginsburg, M. Feldman","doi":"10.51520/2766-838x-10","DOIUrl":"https://doi.org/10.51520/2766-838x-10","url":null,"abstract":"In his manuscript we summarize the role played by the variety of pro inflammatory agents generated by neutrofiles and by macrophages and how these cells may act in synergy to injure human and tissues in various inflammatory and in post inflammatory conditions. Screening the vast published literature, we found that most of papers published on inflammation, tend to stress only the role played by a very few pro inflammatory agents as key agents in inflammation. This is unreasonable to try to understand how leukocytes kill microbial cells and injure mammalian cells in various medical conditions. This is since the PMNs and macrophages produce numerous inflammatory agents.","PeriodicalId":409797,"journal":{"name":"RAS Microbiology and Infectious Diseases","volume":"35 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131465826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}