Antibiotics Resistant Genes and Plasmid curing of Multidrug Resistant Clinical Bacterial Isolates from the Three Geographical Regions of Ondo State, Nigeria

This study shows the characteristics of multidrug resistant clinical isolates analyzed for their antibiotic resistant genes in laboratory. Genomic DNA was then extracted from each sample using Column-pure Bacteria Genomic DNA Isolation kit. Similarly, plasmid profile, plasmid curing and the antibiotic susceptibility profile of thirty two (32) cultured multidrug resistant clinical isolates were analyzed for their antibiotic resistant genes. The bacterial species tested are Acinetobacter haemolyticus, Budricia aquatic, Burkhoderia cepacia, Citrobacter gilleric, Enterobacter gergoriac,Enterobacter agglomerane, Escherichia coli, Escherichia hermamii, Klebsiella oxytoca, Klebsiella ornithinolytica, Klebsiella terrigena, Pseudomonas aeruginosa, Pseudomonas fluorescens, Staphylococcus spp., Staphylococcus aureus and coagulase –ve, Staphylococcus spp. Nutrient agar and Mueller Hinton Agar were used routinely during the study. The genomic test done on the thirty two (32) multidrug resistant strains did not show any resistant gene. But, the molecular plasmid profile conducted shows that all the isolates possess plasmid. Plasmid curing done reduces the antibiotics resistance nature of the isolates. This shows that the resistance of the isolates were plasmid mediated. The re-assessment of the antibiotic susceptibility profile of the clinical bacterial isolates tested confirmed this. It was also clarified in this study that most of the selected resistant bacterial strains isolated from hospitals were susceptible to medicinal plants such as Moringa oleifera and Andrographis peniculata tested on them. This implies that the use of appropriate medicinal plants can be optimized for pharmaceutical purpose to overcome infections caused by these aetiologic agents.