Saudi Journal of Pathology and Microbiology最新文献

{"title":"L-Arginase from P. aeruginosa Isolated from Sewage as Anti-Biofilm Formation by Pathogenic Bacteria","authors":"Zainab Abbas Abd, A. M. Majeed, Zaid Raad Abbas","doi":"10.36348/sjpm.2023.v08i10.003","DOIUrl":"https://doi.org/10.36348/sjpm.2023.v08i10.003","url":null,"abstract":"Background: The enzyme arginase plays a significant role in the growth of many organisms as important biological enzyme. Objective: The study was designed to isolate L-arginase from P. aeruginosa that isolated from sewages and to evaluate the influence of arginase on biofilm-generating bacteria. Materials and Methods: The current study includes 28(54.8%) isolates from soil with 24 (46.2%) from sewage water out of 52 isolated from soil and sewage for the purpose of obtaining Pseudomonas aeruginosa. Using standard laboratory methods to isolate Pseudomonas aeruginosa, 33 isolates were isolated, of which 15 (45.45%) were from soil and 18 (54.54%) from sewage. The study also included 177 different eyes (urinary fluid, excrement, blood, wounds, sputum, and cerebrospinal fluid) of patients suffering from various injuries. Results: The statistical analysis of the isolates indicated that there is a high significant (P≤0.01) for the growth of some types of bacteria, such as P. aeruginosa 30(16.89%), Klebsiella, Serratia 33(18.88%) and Acinetobacter 33(18.88%). While the results showed that Lactobacillus is the lowest number of isolates 7(3.9%). Optimum condition of arginase activity and stability comprising pH level of eight degrees, the temperature of 37℃ is the best conditions for the production of arginine, concentrations of L-arginine substrate (0.1-2.5)g/l on the activity of arginase enzyme for arginine production, The finding also shows that the arginase enzyme exhibited the highest activity after 96 hours of the incubation process L- arginase Purification Assay including Ammonium Sulphate precipitation in (60-80)%, Dialysis, Ion exchange chromatography, Gel filtration. Conclusions: This study concluded that the enzyme arginase had a significant effect on the strong biofilm of pathogenic bacteria.","PeriodicalId":407259,"journal":{"name":"Saudi Journal of Pathology and Microbiology","volume":"59 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139317390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Scrap Cytology: A Screening Shepherd","authors":"Biren P. Modi, Himanshu U. Patel","doi":"10.36348/sjpm.2023.v08i07.004","DOIUrl":"https://doi.org/10.36348/sjpm.2023.v08i07.004","url":null,"abstract":"Scrap cytology is emerging technique for diagnosing oral lesion. Oral cancer results from various viral infections, oral leukoplakia, and submucosal fibrosis. Scrap cytology assist in identifying these malignant and premalignant lesions. Herein, we present a case of a 70 years old male with complaint of pain and multiple ulceration in right oral mucosa. After proper intraoral examination & scrap cytology smears and microbiological investigations diagnosis of HSV cytopathic effect was given. This case report expands the morphologic spectrum of premalignant oral lesions and emphasizes the need to consider scrap cytology as a useful tool for oral lesions.","PeriodicalId":407259,"journal":{"name":"Saudi Journal of Pathology and Microbiology","volume":"220 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122138210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Wnt/β-catenin Signaling in Endometrioid Endometrial Cancer and Precursor Lesions","authors":"Sunita Yadav, A. Makker, Seema Nayak, P. Agarwal, U. Singh, U. Singh, M. Goel","doi":"10.36348/sjpm.2023.v08i08.005","DOIUrl":"https://doi.org/10.36348/sjpm.2023.v08i08.005","url":null,"abstract":"Endometrioid endometrial carcinoma (EEC) is most common invasive malignancy of female genital tract. In spite of advances in diagnosis and treatment, incidence of EEC has been rising because of increasing aging population and prevalence of obesity. Therefore, there is need of research exploring the underlying molecular mechanism of EEC and its precursors. Wnt/ β-catenin signaling are involved in the progression and invasion of EEC. However, the potential role of Wnt/ β-catenin signaling and its effectors in endometrial cancer and precursor lesions remains to be investigated. In the present study a total of 95 samples of endometrial biopsies were assessed histopathologically along with β-catenin expression by immunohistochemistry and promoter methylation of adenomatous polyposis coli (APC) gene by bisulfite method. Women age ranged from 35 to 70 years whose endometrial biopsy and resected samples were obtained. Expression of β-catenin was observed in 28/39; 72% of EEC, 23/37; 62% of precancer cases and 4/19; 21% of normal proliferative endometrium. APC promoter was completely unmethylated in all controls (14/14) and precancer (10/10) groups, however, only one case (1/13, 8%) of cancer was methylated. The present study showed that expression of β-catenin was upregulated in EEC and precursor lesions suggesting involvement of Wnt/ β-catenin signaling from an early stage of development of cancer from precancer. The information might offer a potential therapeutic target for EEC and could also be used as a screening tool in endometrial hyperplasia (EH) cases for detecting potentially precancerous lesions.","PeriodicalId":407259,"journal":{"name":"Saudi Journal of Pathology and Microbiology","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125510448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Duodenal Metastasis from Colorectal Cancer: A Case Report","authors":"A. Chehal, M. Ibrahim, Ashraf Alakkad, Aisha Mohamed Al Salami, H. Sheikh, Deepthi Silymon, S. A. Harthi, Ashish Vittalrao Chintakuntlawar, A. Uttamchandani, Najla Saleh Ben Ghashir","doi":"10.36348/sjpm.2023.v08i08.004","DOIUrl":"https://doi.org/10.36348/sjpm.2023.v08i08.004","url":null,"abstract":"Background: The occurrence of metastatic colon lesions in various organs has been documented extensively in the literature. The liver, lungs, and bones are the most frequently affected sites. Metastasis in the duodenum due to colon cancer is an infrequent occurrence. Case Presentation: We present a detailed case report of a 65-year-old female patient diagnosed with metastatic colon cancer to the duodenum. The patient initially underwent extended right hemicolectomy for stage III low-grade adenocarcinoma of the colon. Adjuvant chemotherapy was administered, resulting in a period of apparent remission. However, in 2020, the patient experienced elevated tumor marker levels, prompting further investigations. Endoscopy revealed an irregular mass infiltrating the muscle layer in the duodenum, confirming malignant involvement. The patient underwent curative distal gastric duodenectomy, with pathology results indicating a colonic origin of the tumor. The patient received first-line palliative chemotherapy with FOLFOX rechallenge and the addition of AVASTIN. Several cycles of chemotherapy were completed, with satisfactory tumor response observed in follow-up PET scans. Maintenance therapy was initiated after achieving disease-free status on CT scans. However, in October 2022, the patient exhibited increasing tumor marker levels and new findings on PET scan, leading to the initiation of second-line palliative therapy with FOLFIRI and ramucirumab. The patient has received multiple cycles of second-line therapy, with the most recent PET scan in February 2023 showing a favorable treatment response, including a decrease in the size of existing nodes and no identification of new lesions. Conclusion: In conclusion, this case exemplifies the journey of a patient with stage 3 colon cancer who initially responded well to surgery and adjuvant chemotherapy, but experienced subsequent relapse in the duodenum. Despite surgical intervention and palliative chemotherapy, the patient developed metastasis in the liver, lung, and multiple lymph nodes.","PeriodicalId":407259,"journal":{"name":"Saudi Journal of Pathology and Microbiology","volume":"180 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124428393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of Histological Prognostic Factors in Colorectal Carcinoma and Relationship of VEGF and E-Cadherin Expression in Prognostic Assessment","authors":"Aysha Femy, Anuradha Rao, Supriya P","doi":"10.36348/sjpm.2023.v08i08.002","DOIUrl":"https://doi.org/10.36348/sjpm.2023.v08i08.002","url":null,"abstract":"Colorectal carcinomas (CRC) are associated with several histopathological and molecular factors of prognostic and hence therapeutic implications, including for epithelial mesenchymal transition (EMT) and angiogenesis. Downregulation of E-cadherin is a crucial step in EMT, directly related to tumor progression, invasion, and metastasis. Vascular endothelial growth factor (VEGF) induces angiogenesis and thereby tumour progression and metastases. Our objective was to analyse VEGF and E-cadherin expression in patients with CRC and to effectively assess the relationship of these with histopathological factors. 50 cases of CRC obtained over eighteen months duration, were included in the study. Haematoxylin and Eosin-stained sections were studied for histopathological factors. Immunohistochemistry with E-Cadherin and VEGF were performed on representative sections and evaluated. Histopathological features of importance noted were low grade tumour budding (55.5%), Infiltrative invasive front (83%), lympho-vascular invasion (50%). Preserved expression of E-Cadherin was seen in 66% of the cases, and 34% reduced expression, predominantly noted in patients above the age of 50, increased tumour size, mucinous variants, advanced TNM stage, lymph node involvement and high-grade tumour budding. VEGF staining was seen in 64% of the cases with varying intensity. Increased VEGF expression was noted in poorly differentiated tumours, advanced Duke’s stage and with lymph node metastasis. Colorectal carcinomas with high grade tumour budding were associated with lymph vascular invasion, infiltrative invasive front and significantly correlated with TNM stage. High grade tumours and adverse histopathological prognostic factors revealed a reduced E-cadherin expression and increased VEGF expression.","PeriodicalId":407259,"journal":{"name":"Saudi Journal of Pathology and Microbiology","volume":"74 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127283174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fibrosarcoma Presenting as Marjolin Ulcer – A Rare Case Report","authors":"Agam Hans, M. Roy, V. Nijhawan","doi":"10.36348/sjpm.2023.v08i08.003","DOIUrl":"https://doi.org/10.36348/sjpm.2023.v08i08.003","url":null,"abstract":"Marjolin ulcer is a rare malignancy that arises in previously traumatised or chronically inflamed skin, particularly after burns, with an average latency period of 36 years. Nearly 75-90% marjolin ulcers are diagnosed as squamous cell carcinoma but other neoplasms like basal cell carcinoma, melanoma, fibrosarcoma etc have also been rarely reported. In this case, a 52-year-old male presented with a painless bleeding mass over lower chest wall rapidly increasing in size from last 2 years. He had suffered burn at the same site 20 years ago. Biopsy was reported as spindle cell neoplasm. Wide local excision revealed a poorly circumscribed subcutaneous spindle cell neoplasm exhibiting variable cellularity, fascicular to storiform architecture, mild to moderate nuclear atypia and scattered gaping thin-walled blood vessels. The neoplastic cells showed diffuse strong staining with CD34 in the less cellular area and patchy positivity in the cellular more mitotically active areas. The tumor cells were immunonegative for EMA, p63, SMA, CD99 and STAT6. The final diagnosis rendered was Dermatofibrosarcoma Protuberans dedifferentiating in to fibrosarcoma. Although sarcoma presenting as a Marjolin ulcer is exceedingly rare, it should be kept in mind in differential diagnosis of spindle cell malignancy arising at a burn site.","PeriodicalId":407259,"journal":{"name":"Saudi Journal of Pathology and Microbiology","volume":"50 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127263719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Utility of Immunofluorescence in Cutaneous Vasculitis","authors":"A. D. Pant, N. Pokharel","doi":"10.36348/sjpm.2023.v08i08.001","DOIUrl":"https://doi.org/10.36348/sjpm.2023.v08i08.001","url":null,"abstract":"Vasculitis may be due to various causes, including connective tissue disorders, medications, and infections. Histopathology shows similar features in different diseases so DIF (direct immunofluorescence) helps to categorize these vasculitic lesions on basis of positivity of different immunoglobulins. In this study, 40 cases of suspected vasculitis were confirmed by histopathology. Females were more commonly affected and the age range was from 9 to 71 years. Among these cases, 21 were immune complex mediated vasculitis of which c3 and IgG were the most commonly found immunoglobulins. Two of these cases were IgA mediated vasculitis. DIF was of great importance for the diagnosis of the disease for appropriate treatment.","PeriodicalId":407259,"journal":{"name":"Saudi Journal of Pathology and Microbiology","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125952879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and Identification of Acinetobacter Spp. from Various Clinical Specimen and their Antibiotic Susceptibility Pattern in PDU Medical College, Rajkot, Gujarat, Western India","authors":"Suhani Gondha","doi":"10.36348/sjpm.2023.v08i07.006","DOIUrl":"https://doi.org/10.36348/sjpm.2023.v08i07.006","url":null,"abstract":"Introduction: Up to 25% of healthy adults exhibit cutaneous colonization by Acinetobacter and are the most common Gram-negative bacteria carried on the skin of hospital personnel. They are opportunistic pathogens causes a number of outbreaks of infections but their predominant role is in intensive care units. Such infections are often difficult to treat because of widespread resistance to the major groups of antibiotics. Materials and Methods: Various samples were collected aseptically and transported immediately to the bacteriology laboratory. The pathogens were identified by standard laboratory procedures including Gram’s staining, motility, culture, colony characters and biochemical reactions. Antimicrobial susceptibility testing was performed by modified Kirby Bauer method as per the CLSI guidelines. Result: 128 (3.11%) Acinetobacter spp. were isolated from 4112 specimens. Out of these, 72 from general wards and 46 from ICU and 10 from opd. Males (59.37%) are predominant than females (40.62%). The isolates sensitivite to Meropenem (83.59%) followed by Pipracillin-tazobactum (66.41%), Tetracycline (58.59%). Maximum resistance was observed to Cefotaxime (93.75%) followed by Ceftazidime (92.19%) & Cefepime (89.06%). Conclusions: Acinetobacter are the “superbugs” of the modern hospital environment causing significant infections in specific patient populations, especially in patients of ICU which are prone to cause infections due to over use of broad spectrum antibiotics. Awareness to maintain good housekeeping, equipment decontamination, strict attention to hand washing, isolation procedures and control of antibiotic usage, especially in high-risk areas, appear most likely measures to control the spread of Acinetobacter spp. in hospitals.","PeriodicalId":407259,"journal":{"name":"Saudi Journal of Pathology and Microbiology","volume":"137 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123742662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Unusual Clinical Presentation of Extranodal Rosai-Dorfman Disease – A Rare Case Report","authors":"Dr. Thejaswini, Dr. Kharidehal Durga, Dr. B. V. Vydehi, Dr. Byna Syam Sundararao, Dr. Vijayalakshmi","doi":"10.36348/sjpm.2023.v08i07.005","DOIUrl":"https://doi.org/10.36348/sjpm.2023.v08i07.005","url":null,"abstract":"Rosai-Dorfman disease is a rare, idiopathic, benign, histiocytic proliferation, usually seen in children and younger adults. Most common site of involvement is cervical lymph node. However, extra nodal manifestations are seen in significant proportion of cases.This case report discusses the unusual presentation of Rosai-Dorfman disease as mass lesion involving bilateral nasal cavities, paranasal sinuses and orbit along with multiple deposits in dura and cervical lymph node.","PeriodicalId":407259,"journal":{"name":"Saudi Journal of Pathology and Microbiology","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122148302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gene Transfer between Human Bacteria and Pets Bacteria","authors":"Hanaa Farhan Abbas, A. Abdulkareem, A. Kadhim","doi":"10.36348/sjpm.2023.v08i07.003","DOIUrl":"https://doi.org/10.36348/sjpm.2023.v08i07.003","url":null,"abstract":"Many individuals are exposed to bacteria especially those who are raising pets. This interaction between household pets such as cats and dogs and human gives the chance for bacteria to transmit easily. Thereby, these bacteria could transfer from pet’s food and scratching to the owners. In this review, a bacteria gene transmission between pets and human is introduced and discussed. This transmission is happened due to the raising and owning these small animals. In addition, studies on Zoonotic diseases transfer between humans and animals especially pets are presented. Furthermore, the risk of pet ownership by people is reviewed and how should they interact with each other. Also, researches on how Antimicrobial-resistant pathogenic bacteria transmitting between human and pets are discussed. Eventually, gene transfer types between human and pet are explained with their consequences.","PeriodicalId":407259,"journal":{"name":"Saudi Journal of Pathology and Microbiology","volume":"67 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126689195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}