Journal of Medical and Life Science最新文献

{"title":"Optimization of hyaluronidase enzyme conditions produced from pseudomonas aerigenosa isolated from diabetic foot ulcer patients","authors":"F. Kadhum","doi":"10.21608/jmals.2023.295417","DOIUrl":"https://doi.org/10.21608/jmals.2023.295417","url":null,"abstract":"Using clinically isolated pseudomonas aerigenosa , the best conditions for making hyaluronidase were studied. The production medium pH7 with 0.5% lactose and yeast extract, a novel bacterial culture (106Cell/ml), and 37 o C for 24 hours produced the maximum hyaluronidase. The specific activity of the hyaluronidase that was generated in the culture medium rose to 130 U/mg of protein as a result of these circumstances.","PeriodicalId":406966,"journal":{"name":"Journal of Medical and Life Science","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131978784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and molecular characterization of arginine deiminase-producing bacteria in Egyptian soil from various soil environments","authors":"M. Kassab","doi":"10.21608/jmals.2023.295732","DOIUrl":"https://doi.org/10.21608/jmals.2023.295732","url":null,"abstract":"","PeriodicalId":406966,"journal":{"name":"Journal of Medical and Life Science","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131361097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochemical studies on the effect of prostaglandin inhibitors and willow bark extract on liver cirrhosis induced by acetylsalicylate in rats","authors":"Doha Beltag, E. Ali, Hager S. Sakr, E. Radwan","doi":"10.21608/jmals.2022.273349","DOIUrl":"https://doi.org/10.21608/jmals.2022.273349","url":null,"abstract":"Prostaglandins (PGs) are arachidonic acid metabolites produced by the action of the enzyme cyclooxygenase (COX). Although PGs are important mediators of inflammation in various diseases, The deciduous herb, Salix mucronata Thunb (commonly called cape silver willow or safsaf willow), is widely distributed along the Nile River in Egypt. Like other willow trees, extracts from safsaf have also been used in traditional medicine. The anti-inflammatory effects of Salix mucronata are the inhibition of cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2) leading to the inhibition of prostaglandin synthesis. The study aims to assess the induced cirrhotic effect of high doses of prostaglandin inhibitors as acetylsalicylate administration on the liver of rats to determine the protective impact of willow bark ( Salix mucronata ) extract on liver cirrhosis in rats with studying","PeriodicalId":406966,"journal":{"name":"Journal of Medical and Life Science","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116436638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of thermostable Streptokinase by recombinant DNA technology as fibrinolytic agent for different thromboembolic disorders","authors":"Kassab Mohammed","doi":"10.21608/jmals.2022.288999","DOIUrl":"https://doi.org/10.21608/jmals.2022.288999","url":null,"abstract":"","PeriodicalId":406966,"journal":{"name":"Journal of Medical and Life Science","volume":"208 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123733657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toxicity of monosodium glutamate intake on different tissues induced oxidative stress: A Review","authors":"Aljohara M. Al-Otaibi, Nahed Mansour, Hala Elabd, Nadeen Esmail","doi":"10.21608/jmals.2022.264345","DOIUrl":"https://doi.org/10.21608/jmals.2022.264345","url":null,"abstract":"","PeriodicalId":406966,"journal":{"name":"Journal of Medical and Life Science","volume":"37 11","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"113983499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HEMATOLOGICAL REFERENCE VALUES FOR HEALTHY ADULTS IN EGYPT","authors":"E. Radwan, D. Emam, Ghada A. Tabl, Noha El- Saidy, A. Elkaaky","doi":"10.21608/jmals.2022.257921","DOIUrl":"https://doi.org/10.21608/jmals.2022.257921","url":null,"abstract":"","PeriodicalId":406966,"journal":{"name":"Journal of Medical and Life Science","volume":"47 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122770752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytotoxicity assessment of silver nanoparticles on liver and spleen of females rats","authors":"Hasan Aljezani, I. Ali","doi":"10.21608/jmals.2022.257163","DOIUrl":"https://doi.org/10.21608/jmals.2022.257163","url":null,"abstract":"Silver nanoparticles (AgNPs) is the most important nano molecules that have many medical, industrial, and cosmetic application. We study the effect of these particles on the most vital organs such as the liver and spleen in Wister rats. AgNPs are characterized by using a scanning probe microscope device (SPM). Twenty females were divided into two groups, the control, and the treated group. All treated female’s gavage 20mg/ml of AgNPs suspension orally for 29 days. The weight of females was 130±5 g. The liver and spleen were used in this study after staining by H&E. The result showed few intracytoplasmic fat vacuoles on liver tissue, on the other hand, the result displayed brown pigment in the sinusoids. The result showed found marked vascular congestion in liver tissue after being treated with AgNPs compared to the control group. The histological examination of the spleen in treated rats showed the presence of brown pigment in the red pulp of the spleen and congestion, and hemorrhage in treated rats. The result showed brown pigments deposited mainly in red pulp compared to the control group. The result presented that this nanoparticle could penetrate the membranes and reaches into many body organs because can penetrate the brain barrier, bloodstream, and many barriers to the harmful effect of acute exposure or chronic exposure to these nanoparticles.","PeriodicalId":406966,"journal":{"name":"Journal of Medical and Life Science","volume":"60 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123474760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical and Hematological changes associated with brucellosis in Missan Province","authors":"Anas Abuali","doi":"10.21608/jmals.2022.256973","DOIUrl":"https://doi.org/10.21608/jmals.2022.256973","url":null,"abstract":"Objective: Brucellosis is still endemic in Iraq and the main source of public health risk; this study was performed to investigate the clinical signs and symptoms of Brucellosis and determine the hematological changes in the patients infected with Brucella which may assist in the rapid diagnosis of the disease. Subjects and Methods: The study was performed in a period extending from (the first of January -2021to to the Third of June-2021). The data and blood samples were collected from (184) outpatients from a big hospital (Al-Sadder) in Missan Province infected with brucellosis aged between (10 - 65) years. the patients were diagnosed with brucellosis according to case history and clinical laboratory examination. The blood sample from each patient was divided into two test tubes, the first (2ml) for serological examination through separated the serum from the blood components and stored at (-18 °C) until analysis by using the Rose Bengal test. The hematological assessment was performed in the laboratory for each sample of blood (3 ml with EDTA) obtained from the patients infected with brucellosis. Complete blood counts were shown from the collected blood samples by automatic methods (System X kx-21n automated hematology analyzer; JAPAN CARE CO., LTD) including Hemoglobin (Hb), Leukocytes or white blood cells (WBCs), Platelets, Lymphocytes, and Neutrophiles. Results: The disease was diagnosed in the patients by using the Rose Bengal test. the results observed a strong positive reaction for the patients infected with brucellosis , the majority and more than half of brucellosis cases were recorded in females (58.3%), and males (41.7%) of all cases. High incidence of brucellosis in the ages between (41-50) years (33.6%) cases, followed by the ages between (31 -40) years (31.1%) cases, while the lowest percentage infected with the disease was reported in ages between (10 -20) years in (8.1%) cases . The most common symptoms observed were fatigue (85.3%), fever (77.7%), arthritis (73.3%), sweats (69%), and headache (64.1%). The hematological analysis for the blood samples observed significant changes in ( P< 0.05) in leukocytes, leukocytosis (23.8%), leucopenia (12.2%), Anemia reported in (42.7%), Platelets also reported in abnormal value, thrombocytosis (15.1%), thrombocytopenia (11.1%) of all cases of brucellosis. lymphocytosis (32.7%), lymphocytopenia (15.5%), while the neutrophils reported in abnormal ranges , neutrophilia (15.5%),neutropenia (11.6%) . Conclusions: the brucellosis prevalence as a zoonotic disease in rural areas in Missan and direct contact and handling of livestock make the females highly exposed to the risk of infection. The blood picture reveals hemolytic anemia, leukopenia, lymphocytosis, lymphocytopenia, and Neutrophils with neutropenia particularly when the disease is epidemiologically suspected. Recommendations : Some of the population tend to rear animals inside the houses, which demanded specific authorities to take care c","PeriodicalId":406966,"journal":{"name":"Journal of Medical and Life Science","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115250014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-tumor Activity of Gold nanoparticles by Use High Content Screening Technique (HCS)","authors":"Ali Al-Dulimia, A. Hasan, Osama A. Al-Mogadamy","doi":"10.21608/jmals.2022.256487","DOIUrl":"https://doi.org/10.21608/jmals.2022.256487","url":null,"abstract":"DOI: 10.21608/jmals.2022.256487 Abstract Gold nanoparticles (GNPs) act as less toxic and effective drug carriers with many specific properties that are particularly useful for biomedical and pharmaceutical applications. The present study was conducted to determine the anti-tumor properties GNPs. The new preparation technique is quick and cost-effective. GNPs formation was confirmed using various characterization techniques including X‐Ray diffraction (XRD) analysis, field emission scanning electron microscopy (FE-SEM), energy dispersive X-ray spectrometer (EDX), and transmission electron microscopy (TEM). XRD, FES-EM, and TEM experiments have confirmed the formation of GNPs which shows that the prepared particles are spherical inform and have a smooth surface with an average size of 24.32 ± 1.32 nm. Multiparametric analysis of GNPs toxicity at the level of individual cells using flow cytometry and cellular imaging-based approaches such as High Content Screening (HCS) have played key roles in the detection of toxicity and classification of compounds based on observed patterns of reversible and irreversible cellular injury. The effect of GNPs on the MCF-7 cell line regarding cell viability, cell nucleus morphology, membrane permeability, potential mitochondrial permeability, and cytochrome C release was further confirmed using a High Content Screening array scan via multi-parametric kit. Results indicated that the viable count of MCF-7 cells was highly decreased by treated with GNPs. Nuclei stained with Hoechst blue appeared to be mostly condensed in response to the treatment with the GNPs (1.5fold increase in nuclear fluorescence intensity), with significant differences (p < 0.0263) as compared with untreated cells. Cell membrane permeability was significantly (p = 0.0019) upon GNPs showing an increase of 1.9-fold, as compared with the untreated MCF-7 cells. GNPs induce a significant (p = 0.0053) decrease in mitochondrial membrane potential by 32.7%, in comparison to the untreated cancer cells with significant induction of the release of cytochrome C (p = 0.0016) was recorded after exposure to the GNPs (0.43-fold increased mean intensity), The outcomes of the present study propose the high feasibility for using GNPs as a novel anticancer drug.","PeriodicalId":406966,"journal":{"name":"Journal of Medical and Life Science","volume":"41 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116036362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retrocaval Ureter: A Case and Review of the Literature","authors":"Farouk Hachem, H. Abdallah, A. Ibrahimi, H. Elsayegh, Y. Nouini","doi":"10.21608/jmals.2022.245386","DOIUrl":"https://doi.org/10.21608/jmals.2022.245386","url":null,"abstract":"The retrocaval ureter is a rare disease found most often in young adult males, the usual clinical manifestation is right lumbar pain due to obstruction of the upper urinary tract. The diagnosis is based on CT and the treatment is mainly laparoscopic as our observation shows.","PeriodicalId":406966,"journal":{"name":"Journal of Medical and Life Science","volume":"443 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116513792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}