Optimization of hyaluronidase enzyme conditions produced from pseudomonas aerigenosa isolated from diabetic foot ulcer patients

Abstract

Using clinically isolated pseudomonas aerigenosa , the best conditions for making hyaluronidase were studied. The production medium pH7 with 0.5% lactose and yeast extract, a novel bacterial culture (106Cell/ml), and 37 o C for 24 hours produced the maximum hyaluronidase. The specific activity of the hyaluronidase that was generated in the culture medium rose to 130 U/mg of protein as a result of these circumstances.