Journal of Sugarbeet Research最新文献

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Fungicide sensitivity characteristics of Cercospora beticola isolates recovered from the high plains of Colorado, Montana, Nebraska, and Wyoming. 1. Benzimidazole and triphenyltin hydroxide
Journal of Sugarbeet Research Pub Date : 2001-04-01 DOI: 10.5274/JSBR.38.2.111
S. Brière, G. Franc, E. Kerr
{"title":"Fungicide sensitivity characteristics of Cercospora beticola isolates recovered from the high plains of Colorado, Montana, Nebraska, and Wyoming. 1. Benzimidazole and triphenyltin hydroxide","authors":"S. Brière, G. Franc, E. Kerr","doi":"10.5274/JSBR.38.2.111","DOIUrl":"https://doi.org/10.5274/JSBR.38.2.111","url":null,"abstract":"The appearance of Cercospora beticola isolates with insensitivity to triphenyltin hydroxide (TPTH) or benzimidazole (BM) fungicides is a problem in many sugarbeet growing areas of the world including the United States. In 1998 and 1999 sugarbeet growing areas in northeastern Colorado, southeastern Montana and southwestern Nebraska were surveyed to determine if insensitive isolates were present. One field in southeast Wyoming was included in the 1999 survey. There were 328 isolates recovered from 110 fields in 1998 and 305 isolates from 101 fields in 1999. Radial growth of isolates on potato dextrose agar amended with 1, 5, and 10μg mL -1 TPTH and 5μg mL -1 BM was compared to growth in the absence of fungicide. Percent inhibition of radial growth in the presence of 1 μg mL -1 TPTH ranged from 17% to 100% in 1998 and 37% to 100% in 1999. More inhibition of radial growth was observed as the concentration of TPTH increased. Results for 5.0μg mL -1 BM revealed that 72 of the 328 isolates grew with 20% or less inhibition in 1998 and 87 of 305 isolates grew with 40% or less inhibition in 1999. Insensitivity to BM is more pronounced in Colorado and Nebraska when compared to Montana, presumably due to greater fungicide use in the more southerly production areas. Data reported here establishes baseline characteristics of the current C. beticola population.","PeriodicalId":403165,"journal":{"name":"Journal of Sugarbeet Research","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2001-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122366642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 14
Production and performance of gynogenetic sugarbeet lines. 母性甜菜品系的生产与性能。
Journal of Sugarbeet Research Pub Date : 2000-10-01 DOI: 10.5274/JSBR.37.4.117
A. Svirshchevskaya, J. Doležel
{"title":"Production and performance of gynogenetic sugarbeet lines.","authors":"A. Svirshchevskaya, J. Doležel","doi":"10.5274/JSBR.37.4.117","DOIUrl":"https://doi.org/10.5274/JSBR.37.4.117","url":null,"abstract":"In vitro techniques were used to obtain haploid and doubled haploid lines of gynogenetic origin in sugarbeet (Beta vulgaris L.). Two pathways of regeneration were available for unfertilized ovule culture - embryogenesis and organogenesis. Regenerants were obtained from primary explants of diploid and tetraploid donor plants on MS nutrient medium supplemented with benzylaminopurine and sucrose. Flow cytometry indicated spontaneous polyploidization in all primarily haploid ovule-derived lines that originated from Belarusian cultivars. Mixoploidy that occured in doubled haploids was overcome through micropropagating apices of generative shoots. Performance of sugarbeet plants of ovule origin and hybrids produced with these lines indicated that promising breeding lines could be selected from gynogenetic lines.","PeriodicalId":403165,"journal":{"name":"Journal of Sugarbeet Research","volume":"2012 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2000-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130677974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 15
Mitochondrial DNA variation in maintainer lines (O-type) of sugarbeet. 甜菜维持系(o型)线粒体DNA变异。
Journal of Sugarbeet Research Pub Date : 2000-10-01 DOI: 10.5274/JSBR.37.4.153
Z. Sadoch, R. Wierzchosławski, T. Panczyk
{"title":"Mitochondrial DNA variation in maintainer lines (O-type) of sugarbeet.","authors":"Z. Sadoch, R. Wierzchosławski, T. Panczyk","doi":"10.5274/JSBR.37.4.153","DOIUrl":"https://doi.org/10.5274/JSBR.37.4.153","url":null,"abstract":"The mitochondrial genome influences the cytoplasmic male sterile trait in sugarbeet (Beta vulgaris L.); therefore, comparisons of mitochondrial DNA structure and organization of sterile and normal cytoplasm can elucidate the molecular basis of male sterility. Mitochondrial DNA variability in maintainer lines of sugarbeet was characterized by restriction fragment length polymorphisms (RFLP). Mitochondrial DNA of four lines from a North American monogerm population and six lines from a Russian monogerm population were restricted with EcoRI and hybridized with coxII and atpA probes. EcoRI digestion revealed three distinct restriction patterns: one typical of the pattern for normal cytoplasm of sugarbeet and two that resembled patterns for mtDNA from fertile plants of wild beet, B.v. ssp. maritima. The probing of mtDNA with coxII revealed two mitochondrial variants differing in a 1.8 kbp hybridization signal. The atpA probe identified alterations of mitochondrial DNA which resulted either in the appearance of a new 3.5 kbp band or in the absence of a 4.4 kbp band and the presence of 3.5 kbp signal. Changes in mitochondrial genome organization were observed only in maintainer lines from the Russian population. These differences in mitochondrial DNA provide evidence for the existence of two unique fertile cytoplasms for use in the development of male-fertile sugarbeet breeding lines. RFLP analysis and molecular characterization of mitochondrial DNA of male-fertile germplasm provides breeders useful information on genetic diversity within O-type lines. Maintainer lines with unique mtDNA can be used to diversify sugarbeet germplasm.","PeriodicalId":403165,"journal":{"name":"Journal of Sugarbeet Research","volume":"54 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2000-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121514775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cytological and Karyotypic Studies in Four Beta Species 四种β的细胞学和核型研究
Journal of Sugarbeet Research Pub Date : 2000-10-01 DOI: 10.5274/JSBR.37.4.135
S. Srivastava, H. M. Srivastava
{"title":"Cytological and Karyotypic Studies in Four Beta Species","authors":"S. Srivastava, H. M. Srivastava","doi":"10.5274/JSBR.37.4.135","DOIUrl":"https://doi.org/10.5274/JSBR.37.4.135","url":null,"abstract":"The karyotype and its components have been used to decipher karyo-evolutionary trends within taxa. With this in mind, chromosomal characteristics of four species of the genus Beta were studied to find karyotype relation­ ships, vis-a-vis meiotic features, and to ascertain the feasibility of using these species for development of inter­ specific hybrids and polyploids in sugarbeet (B. vulgaris L.) breeding programs in subtropical India. Four species of Beta were studied; B. vulgaris L., B. vulgaris ssp. maritima, B. vulgaris ssp. oriel/talis, and B. lomantogona; all with chromosome numbers of2n=18. Their karyotypes were generally asymmetric. Total haploid chromatin length ranged from 17.92 to 24.17 ~m, whereas individual chromosome size ranged from 1.47 to 3.15 ~m. According to Stebbin's classification of asymmetry, these species ranged from 2A to 4A, thereby confirming an evolution­ ary trend among the karyotypes. The karyotype of B. vul­ garis L. var LS-6 was most advanced and was classified as 4A. The karyotypes of B. vulgaris ssp. maritima and ssp. orientalis were classified as 3A. To further quantify gradations within a class of asymmetry, a chromosome Dispersion Index (DI) was calculated. A DI value of 0.479 for B. vulgaris L. var LS-6 confirmed its high degree of karyotypic specialization. Meiotically, all the species formed predominantly open bivalents with distal chiasma localization. Chiasma formation per bivalent decreased as the length of pairing blocks increased. This suggested a species specific chromosome condensation gradient because sugarbeet karyotypes are relatively constant during somatic and meiotic phases.","PeriodicalId":403165,"journal":{"name":"Journal of Sugarbeet Research","volume":"34 4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2000-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132573500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Beta breeding and genetics at East Lansing, Michigan: molecular methods, genetic diversity, and trait elucidation. 密歇根州东兰辛的β育种和遗传学:分子方法,遗传多样性和性状阐明。
Journal of Sugarbeet Research Pub Date : 2000-10-01 DOI: 10.5274/JSBR.37.4.97
J. McGrath, B. G. Reyes, J. Saunders
{"title":"Beta breeding and genetics at East Lansing, Michigan: molecular methods, genetic diversity, and trait elucidation.","authors":"J. McGrath, B. G. Reyes, J. Saunders","doi":"10.5274/JSBR.37.4.97","DOIUrl":"https://doi.org/10.5274/JSBR.37.4.97","url":null,"abstract":"Variety and germplasm development and release have been the main goals of the USDA-ARS sugarbeet (Beta vulgaris L.) breeding program at East Lansing, MI for over 70 years. Progress has been made in improving tolerance to Aphanomyces seedling disease, Cercospora leaf spot tolerance, Rhizoctonia crown and root rot tolerance, herbicide resistance, and low-tare (smooth root) root morphology. The genetic basis of these traits is not well understood, and the current program seeks to identify genes that influence the expression of disease, quality, and morphological traits, and locate them on beet chromosomes. The out-crossing nature of sugarbeet is not well suited for large-scale genetic analyses. A strategy has been adopted that should allow genetic dissection of a variety of traits through standard genetic analyses. Briefly, a genetic male sterile seed parent that also carries a dominant self-fertility gene is paired with germplasm of interest. The resulting self-fertile hybrid is self-pollinated to produce a segregating F 2 population, which is simultaneously observed for segregation of targeted traits and tested for linkage of these traits using molecular markers. Segregation of male-sterility and self-fertility genes in the F 2 gives a range of options for further characterization. In addition, this strategy allows relatively rapid introduction of traits from wild and unadapted germplasm while simultaneously determining the genetic basis for novel traits introduced through such crosses. Using this system will allow systematic exploration of linkage relation-ships between agronomic genes from diverse germplasm sources and molecular markers.","PeriodicalId":403165,"journal":{"name":"Journal of Sugarbeet Research","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2000-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114409378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Localization of New Monogerm and Late-bolting Genes in Sugarbeet using RFLP Markers ' 利用RFLP标记定位甜菜新单、晚抽基因
Journal of Sugarbeet Research Pub Date : 2000-10-01 DOI: 10.5274/JSBR.37.4.107
Y. Shavrukov
{"title":"Localization of New Monogerm and Late-bolting Genes in Sugarbeet using RFLP Markers '","authors":"Y. Shavrukov","doi":"10.5274/JSBR.37.4.107","DOIUrl":"https://doi.org/10.5274/JSBR.37.4.107","url":null,"abstract":"A single M-m gene controlling multi-monogerm pheno­ type has been described and mapped into linkage group 2. In this study a second monogerm locus, designated m 2 , was analyzed in a segregating population from a cross between monogerm and multigerm lines of Russian ori­ gin (S22 and No.14, respectivel)'). The 11/ 2 gene was located using RFLP markers previously mapped in the sugarbeet (Beta vulgaris L.) genome. Seventeen RFLP probes were selected and 56 F2 plants examined. Nine RFLP markers were located in all linkage groups except group 2. Segre­ gation patterns of these nine RFLP markers and the monogerm and late-bolting traits indicated independence. The other eight RFLP probes indicated an association between monogerm and late-bolting in linkage group 2. The linear order of the markers considered in this analy­ sis was identical to the order reported by others. How­ ever, the distance between some RFLP markers was not consistent with previous reports. The m 2 gene was mapped in the same linkage group (group 2) as the m gene. The distance between the m 2 and m genes was 32.2 cM. These results confirmed our supposition, based on genetic ex­ periments, that these genes are different from each other. The lb gene from the Russian lines and the late-bolting gene reported in other germplasm appeared to be identi­ cal.","PeriodicalId":403165,"journal":{"name":"Journal of Sugarbeet Research","volume":"133 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2000-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127300018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
Cytological and genetic investigation of abnormal pollen development in sugarbeet. 甜菜花粉发育异常的细胞学和遗传学研究。
Journal of Sugarbeet Research Pub Date : 2000-10-01 DOI: 10.5274/JSBR.37.4.143
A. Mglinets
{"title":"Cytological and genetic investigation of abnormal pollen development in sugarbeet.","authors":"A. Mglinets","doi":"10.5274/JSBR.37.4.143","DOIUrl":"https://doi.org/10.5274/JSBR.37.4.143","url":null,"abstract":"Six unique sugarbeet (Beta vulgaris L.) mutations (al, a3, a4, a5, a6, and ap) causing abnormal pollen development were investigated. Cytological examinations of al and a6 indicated that the male sterility caused by these mutations was due to degradation of microspores after initiation of pollen grain wall formation. Genetic investigations of al and a6 demonstrated that these mutations were non-allelic nuclear genes. Mutations a3 and a4 also resulted in male sterility and had monogenic inheritance. The male sterility controlled by a5 is inherited as a digenic character. Cytological observations of a3, a4, and a5 indicated that even though microspore degeneration begins during pollen wall formation for all three, the three mutations were not identical. The ap mutation (accreting pollen) was inherited as a single recessive nuclear gene causing fertile pollen grains to aggregate in the tetrads. All six mutations may be useful in molecular studies of pollen cell wall development. The ap mutation allows tetrad analysis in sugarbeet and the male sterile mutants may have utility in situations where hand emasculation is now required.","PeriodicalId":403165,"journal":{"name":"Journal of Sugarbeet Research","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2000-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129414783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Exploiting novel sources of disease resistance in Beta germplasm using molecular markers 利用分子标记技术开发β种质抗病新来源
Journal of Sugarbeet Research Pub Date : 2000-10-01 DOI: 10.5274/JSBR.37.4.89
S. Francis, M. Asher
{"title":"Exploiting novel sources of disease resistance in Beta germplasm using molecular markers","authors":"S. Francis, M. Asher","doi":"10.5274/JSBR.37.4.89","DOIUrl":"https://doi.org/10.5274/JSBR.37.4.89","url":null,"abstract":"Novel disease resistance genes derived from Beta germplasm can be used to improve the durability and effectiveness of disease resistance in commercial sugarbeet (Beta vulgaris L.) cultivars. In order to introgress these genes into sugarbeet, information on their chromosomal location and possible allelic relationships with other genes is required. This information can be provided by mapping the genes using molecular markers. However, to date, few resistance genes have been mapped in sugarbeet compared with other crops. Besides mapping resistance genes, molecular markers can also be used to instigate map-based cloning of genes and for marker-assisted selection of desirable genotypes in a breeding program. We have identified sources of novel resistance genes in a Beta germplasm collection and developed segregating mapping populations. The locations of these genes in the sugarbeet genome are being determined using amplified fragment length polymorphism molecular markers. Mapping a variety of resistance genes acting against different diseases should contribute significantly to our understanding of the locations of resistance genes in sugarbeet.","PeriodicalId":403165,"journal":{"name":"Journal of Sugarbeet Research","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2000-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124056699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 11
The synthetic Beta core collection - state of the art 合成Beta核心系列,最先进的
Journal of Sugarbeet Research Pub Date : 2000-07-01 DOI: 10.5274/JSBR.37.3.1
L. Frese
{"title":"The synthetic Beta core collection - state of the art","authors":"L. Frese","doi":"10.5274/JSBR.37.3.1","DOIUrl":"https://doi.org/10.5274/JSBR.37.3.1","url":null,"abstract":"More than 10,000 Beta accessions are maintained in genebanks worldwide. Beta core collection accessions were designated to facilitate access to useful genetic variation within large holdings. A set of core collection accessions can be selected by different procedures, depending on the crop and the type of available data. The branching and path indicator (PI) method has been used to establish a Beta core collection consisting of 805 accessions. The core collection currently is being screened for resistance to eight diseases and for drought tolerance within a collaborative project funded by the Commission of the European Countries. The central project database contains 2,013 evaluation results, expressed on a I to 9 scale. In 122 events, accessions were scored equal to or lower than '3' (= very low to low susceptibility). The preliminary analysis of the evaluation data detected 60 of the 122 events in P1132 (B. vulgaris ssp. maritima) followed by 21 events in P1135 (B. vulgaris ssp. vulgaris Garden Beet').","PeriodicalId":403165,"journal":{"name":"Journal of Sugarbeet Research","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2000-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132957052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
Disease Resistance in Collections of Beta Species β种的抗病研究
Journal of Sugarbeet Research Pub Date : 2000-07-01 DOI: 10.5274/JSBR.37.3.39
M. Luterbacher, J. M. Smith, M. Asher, L. Frese
{"title":"Disease Resistance in Collections of Beta Species","authors":"M. Luterbacher, J. M. Smith, M. Asher, L. Frese","doi":"10.5274/JSBR.37.3.39","DOIUrl":"https://doi.org/10.5274/JSBR.37.3.39","url":null,"abstract":"In a collaborative EU-funded program (GENRES CT95­ 42), up to 600 Beta accessions are being screened for resis­ tance to several important diseases ofsugarbeet (Beta vul­ garis ssp. vulgaris) including beet mild yellowing virus (BMYV), beet yellows virus (BYV), Aphanomyces cochlioides, Pythium ultimum and powdery mildew (Erysiphe betae). Results are promising, with between 0.3 and 16% of accessions exhibiting high levels of resistance. A preliminary analysis ofBMYV,Aphallomyces, and pow­ dery mildew data indicates some differences in disease resistance among Beta types. Early testing of progeny de­ rived from plants highly resistant to BMYV and powdery mildew has shown that these traits are heritable.","PeriodicalId":403165,"journal":{"name":"Journal of Sugarbeet Research","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2000-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122000491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 16
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