Malaysian Journal of Medicine and Health Sciences最新文献_第2页

Schwann Cells Reprogram Into Repair Phenotype Instead of Dedifferentiating to Immature Phenotype in in Vitro Culture 雪旺细胞在体外培养中重编程为修复型而非去分化为未成熟型

Malaysian Journal of Medicine and Health Sciences Pub Date : 2023-08-10 DOI: 10.47836/mjmhs.19.s9.16

Nurul Husna Abd Razak, Jalilah Idris, Nur Hidayah Hassan, Fazlin Zaini, Noorzaid Muhamad, Muhammad Fauzi Daud

{"title":"Schwann Cells Reprogram Into Repair Phenotype Instead of Dedifferentiating to Immature Phenotype in in Vitro Culture","authors":"Nurul Husna Abd Razak, Jalilah Idris, Nur Hidayah Hassan, Fazlin Zaini, Noorzaid Muhamad, Muhammad Fauzi Daud","doi":"10.47836/mjmhs.19.s9.16","DOIUrl":"https://doi.org/10.47836/mjmhs.19.s9.16","url":null,"abstract":"Introduction: In vitro cultured Schwann cell has been suggested to adopt a phenotype of undifferentiated immature Schwann cells found in vivo during development. However, recent studies indicate that Schwann cells undergo cellular reprogramming into the phenotype of repair Schwann cells instead of reverting to an immature phenotype after peripheral nerve injury. The study hypothesized that in in vitro culture, Schwann cells assume the repair phenotype instead of de-differentiating to immature Schwann cells, similar to in vivo nerve injury response. Therefore, this study aimed to characterize the phenotype of cultured Schwann cells by examining the expression of classic Schwann markers and transcription factors c-Jun and Krox-20. Methods: Schwann cells, isolated from Wistar rat sciatic nerve, were grown in a standard Schwann cell growth medium for seven days. Then, cultured Schwann cells were analyzed using immunofluorescence analysis for classic Schwann cell markers (neurotrophin receptor p75 (p75NTR) and myelin basic protein (MBP)) and the expression profile of transcription factor c-Jun and Krox-20. Results: Immunofluorescence analysis revealed that cultured Schwann cells expressed a significantly high level of repair phenotype biomarkers (p75NTR and c-Jun) compared to the level of myelinating phenotype biomarkers (MBP and Krox-20). Conclusion: Schwann cells reprogram into repair Schwann cells instead of de-differentiating to immature Schwann cells in vitro.","PeriodicalId":40029,"journal":{"name":"Malaysian Journal of Medicine and Health Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135598389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of Photosensitive Hydrogel-based 3-dimensional Bioprinting Using Locally Extracted Pectin From Durian Rind Waste and Cellulose for Pharmaceutical Application 利用当地提取的榴莲果胶和药用纤维素进行光敏水凝胶生物三维打印的研究

Malaysian Journal of Medicine and Health Sciences Pub Date : 2023-08-10 DOI: 10.47836/mjmhs.19.s9.23

Nor Sorfia Alisha Mohd Kamal, Siti Fatimah Zaharah Mohd Fuzi, Mohd Ifwat Mohd Ghazali, Joe Dailin Daniel

{"title":"Development of Photosensitive Hydrogel-based 3-dimensional Bioprinting Using Locally Extracted Pectin From Durian Rind Waste and Cellulose for Pharmaceutical Application","authors":"Nor Sorfia Alisha Mohd Kamal, Siti Fatimah Zaharah Mohd Fuzi, Mohd Ifwat Mohd Ghazali, Joe Dailin Daniel","doi":"10.47836/mjmhs.19.s9.23","DOIUrl":"https://doi.org/10.47836/mjmhs.19.s9.23","url":null,"abstract":"Introduction: Hydrogels have gained prominence in a variety of fourth industrial revolution applications, including three-dimensional (3D) printing. However, there are limitations to 3D manufacturing, such as deformities in the final product. This is a significant obstacle to adopting this technology in the pharmaceutical industry, as printed products may have insufficient mechanical properties and a high brittleness, making further processing of these dosage forms problematic. The objective of this study is to produce a new 3D bioink from a mixture of locally produced pectin-based material from durian rind waste and cellulose-based material (pectin/cellulose hydrogel) and to partially characterize the bioink hydrogel. Methods: Four formulations of pectin/cellulose-based hydrogel (3:1, 3:2, 4:1, and 5:3 ratio of pectin/cellulose) from durian rind waste and carboxymethyl cellulose (CMC) powder, as well as cross-linking agents, were developed and evaluated using a rheometer to evaluate viscoelastic properties, FTIR Spectroscopy to identify compounds, and thermogravimetric analysis (TGA) to evaluate thermal stability. Results: All bioink formulations exhibit outstanding shear-thinning behavior suitable for 3D printing. The viscosity of edible ink increases as the pectin/cellulose concentration increases. The formulation of 3:1 pectin/cellulose has greater heat resistance than others (highest thermal stability with 21.69% of residual weight) and the lowest percentage of weight loss (76.18%). Conclusion: The study of a pectin/cellulose hydrogel mixture provides an attractive outcome for the creation of bioink due to the effective synthesis of 3D printing shapes that are both smooth and uniform.","PeriodicalId":40029,"journal":{"name":"Malaysian Journal of Medicine and Health Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135598641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Weight Status, Dietary Intake, and Mealtime Behaviour Among Autism Spectrum Disorder Children in Klang Valley, Malaysia 马来西亚巴生谷自闭症谱系障碍儿童的体重状况、饮食摄入和用餐行为

Malaysian Journal of Medicine and Health Sciences Pub Date : 2023-08-10 DOI: 10.47836/mjmhs.19.s9.9

Nur Hidayah Ghazali, Siti Sabariah Buhari, Kartini Ilias

{"title":"Weight Status, Dietary Intake, and Mealtime Behaviour Among Autism Spectrum Disorder Children in Klang Valley, Malaysia","authors":"Nur Hidayah Ghazali, Siti Sabariah Buhari, Kartini Ilias","doi":"10.47836/mjmhs.19.s9.9","DOIUrl":"https://doi.org/10.47836/mjmhs.19.s9.9","url":null,"abstract":"Introduction: Nutrition is one of the important contributors to the well-being of children with disability. Given the various data on the nutritional status of autism spectrum disorder (ASD) children, it is imperative to conduct a detailed nutritional assessment of the local population. This study compares ASD with typical developing (TD) children to determine the weight status and association of mealtime behavior with dietary intake in Klang Valley, Malaysia. Methods: A total of 106 children aged five to nine years old participated in this cross-sectional study. Sociodemographic were assessed through self-administered questionnaires. The body weight and height were collected; the body mass index (BMI) was calculated. A three-day food record and a Brief Autism Mealtime Behavior Inventory (BAMBI) data were obtained from the caretakers. Results: More than one-third (39%) of the ASD children were overweight or obese compared to TD children (23%). However, there is a significant difference (X2=10.29, p=0.03) when TD children were categorized as stunted and severely stunted. Meanwhile, the total energy intake was statistically significant (X2=9.53, p=0.008). The majority (88.2%) of ASD children experience problems in mealtime behavior characteristically limited food acceptance with a mean score of (28.78±6.80). Mealtime behavior was not associated with energy, carbohydrate, protein, and fat when the p>0.05. Conclusion: Even though there was no association of mealtime behavior with dietary intake, the BMI was significantly influenced by excessive daily calorie consumption. Because of growth parameters influence physical development, nutritional intervention program should be carried out in order to improve nutritional status of AS children.","PeriodicalId":40029,"journal":{"name":"Malaysian Journal of Medicine and Health Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135598778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Telehealth Improves Pregnancy Health Care: Literature Review 远程医疗改善妊娠保健:文献综述

Malaysian Journal of Medicine and Health Sciences Pub Date : 2023-08-10 DOI: 10.47836/mjmhs.19.s9.38

Priyani Haryanti, Santhna Letchmi Pandugaran, Musheer Aljaberi, Ruma Poddar, Mehru Nisha

引用次数: 0

Illness Perception and Self-management of Elderly With Hypertention 老年高血压患者的疾病认知与自我管理

Malaysian Journal of Medicine and Health Sciences Pub Date : 2023-08-10 DOI: 10.47836/mjmhs.19.s9.14

Elizabeth Ari Setyarini, Agnes Monica, Friska Sinaga, FX Widiantoro, Lidwina Triastuti Listianingsih, Cindiana Cecilia

{"title":"Illness Perception and Self-management of Elderly With Hypertention","authors":"Elizabeth Ari Setyarini, Agnes Monica, Friska Sinaga, FX Widiantoro, Lidwina Triastuti Listianingsih, Cindiana Cecilia","doi":"10.47836/mjmhs.19.s9.14","DOIUrl":"https://doi.org/10.47836/mjmhs.19.s9.14","url":null,"abstract":"Introduction: One of the degenerative diseases that affects the elderly is hypertension, which can impact social interactions, quality of life, and mental health. The perceptions of hypertensive patients who are still diverse are closely related to the way they deal with the disease. The purpose of this study is to identify correlation between illness perception and self-management of elderly with hypertension. Methods: Quantitative method with cross sectional design was approached in this study. 125 participants were involved by using purposive sampling technique. The Brief Illness Perception Questionnaire (Brief IPQ) and Hypertension Self-Management Behavior Questionnaire (HSMBQ) were used to measure illness perception and self-management. The correlation of two variables was analyzed by using Pearson Product Moment. Results: The results showed that the average score of illness perception in the elderly with hypertension was 50.34 (range 0-80) and self-management was 71.18 (range 0-100). Illness perception significantly positively correlated with hypertension self-management of elderly with hypertension (p value < 0.000, r = 0.590), which it means higher the illness perception, it will improve the self-management. Conclusion: This result could be used as data to provide sources of information to the elderly with hypertension to further improve the perception of the disease in relation to daily self-management so that the elderly remain productive.","PeriodicalId":40029,"journal":{"name":"Malaysian Journal of Medicine and Health Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135598908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Arginine-depleting Enzymes, A Potential Treatment Option for Tumors With Arginine Auxotrophy : A Review 精氨酸消耗酶:精氨酸营养不良肿瘤的潜在治疗选择

Malaysian Journal of Medicine and Health Sciences Pub Date : 2023-08-10 DOI: 10.47836/mjmhs.19.s9.42

Nurhanis Syafiqah Mohd Nor Hamin, Kok Chang Lee, Wen Nee Tan, Woei Yenn Tong, Chean Ring Leong

{"title":"Arginine-depleting Enzymes, A Potential Treatment Option for Tumors With Arginine Auxotrophy : A Review","authors":"Nurhanis Syafiqah Mohd Nor Hamin, Kok Chang Lee, Wen Nee Tan, Woei Yenn Tong, Chean Ring Leong","doi":"10.47836/mjmhs.19.s9.42","DOIUrl":"https://doi.org/10.47836/mjmhs.19.s9.42","url":null,"abstract":"The World Health Organization reports that one of the top global causes of illness and mortality is cancer, with nearly 10 million deaths in 2020. Changes in cellular metabolism are common characteristics of a wide variety of malignancies. Enzymatic deficits cause many tumors to lose the ability to synthesize amino acids required for their growth, survival, or proliferation. Thus, some tumors depend on the extra-cellular supply of specific amino acids to meet their needs, allowing them to survive. Amino acid depletion as a targeted therapy takes advantage of these tumor traits by depleting certain amino acids in the body that is required for the tumor to survive. This review aims to discuss the potential and challenges of arginine-depleting enzymes as a means in treating arginine auxotrophic cancers. Previously, arginine deiminase (ADI) of bacterial origin has been studied for the in vivo arginine auxotrophic tumour therapy. However, it has been hampered by drawbacks, including immunogenicity and toxicity issues. Thus, human arginase I (hARGI) has been considered a better candidate due to its low mmunogenicity and toxicity effects. However, hARGI’s application as an anti-cancer drug is hindered by its low activity towards arginine owing to its high Km values indicating the enzyme’s low substrate affinity. Thus, it is necessary to improve the enzyme catalytic capability and stability for more practical application in therapeutic cancer treatment. With the advancement of bioinformatics tools, more studies are anticipated to rationally engineer the enzyme for more practical clinical application in the treatment of arginine auxotrophic cancers.","PeriodicalId":40029,"journal":{"name":"Malaysian Journal of Medicine and Health Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135598909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Essential Oils of Garcinia spp. and Their Biological Activities 藤黄属植物精油及其生物活性研究

Malaysian Journal of Medicine and Health Sciences Pub Date : 2023-08-10 DOI: 10.47836/mjmhs.19.s9.39

Wen-Nee Tan, Juzaili Azizi, Nurul Awanis Che Omar, Chean-Ring Leong, Woei-Yenn Tong

引用次数: 0

The Practice of Herbs Integration Among Patients With Hypertension Attending Primary Care Facilities: A Systematic Review 高血压患者在初级保健机构中草药整合的实践:一项系统综述

Malaysian Journal of Medicine and Health Sciences Pub Date : 2023-08-10 DOI: 10.47836/mjmhs.19.s9.37

Joe Joachimdass Raphael, Chin Hai Teo, Kavitha Subramaniam, Annaletchumy Loganathan

{"title":"The Practice of Herbs Integration Among Patients With Hypertension Attending Primary Care Facilities: A Systematic Review","authors":"Joe Joachimdass Raphael, Chin Hai Teo, Kavitha Subramaniam, Annaletchumy Loganathan","doi":"10.47836/mjmhs.19.s9.37","DOIUrl":"https://doi.org/10.47836/mjmhs.19.s9.37","url":null,"abstract":"Introduction: The practice of integrating herbs among patients with hypertension to manage their condition has become more evident across many countries. This systematic review was conducted primarily to determine the prevalence and diversity of herbs used by patients with hypertension attending primary care facilities. Data Sources: Studies were obtained from five electronic databases (September 2019) and references of included studies. Review Methods: Studies performed on patients with hypertension attending outpatient clinics and hospitals that reported prevalence or diversity of herbs were included. Two independent researchers performed screening, quality appraisal and data extraction. Results: Sixteen studies were included in this review. The prevalence of herbs used ranged from 6.5% to 69.0%. The two most common herbs reported were Allium sativum and Vernonia amygdalina. The main reasons patients’ took herbs were to reduce blood pressure, to relieve symptoms of the disease and perception that allopathic medicine was a failure. Age, education level, presence of other family members with hypertension and gender were significantly associated (p<0.05) with the use of herbs. Conclusion: Patients with hypertension who attend outpatient clinics tend to use herbs to complement their allopathic medicine. Further evaluation in the form of randomized controlled trials should be conducted to determine the effectiveness of herbs and herbal medicine in improving hypertension among patients.","PeriodicalId":40029,"journal":{"name":"Malaysian Journal of Medicine and Health Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135598775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of the Number of Bacterial Colonies Before and After the Use of Natural Hand Sanitizers Mixed With Betel Leaf Decoction (Piper betle L) and Lemongrass (Cymbopogon nardus L) 槟榔叶煎液与柠檬草天然洗手液混合使用前后菌落数的比较

Malaysian Journal of Medicine and Health Sciences Pub Date : 2023-08-10 DOI: 10.47836/mjmhs.19.s9.2

Bastian Bastian, Rosmiarti Rosmiarti, Yuniza Yuniza, Heri Shatriadi, Murbiah Murbiah, Riska Marlin, Imam Haryoko, Mehru Nisha, Sandeep Poddar

{"title":"Comparison of the Number of Bacterial Colonies Before and After the Use of Natural Hand Sanitizers Mixed With Betel Leaf Decoction (Piper betle L) and Lemongrass (Cymbopogon nardus L)","authors":"Bastian Bastian, Rosmiarti Rosmiarti, Yuniza Yuniza, Heri Shatriadi, Murbiah Murbiah, Riska Marlin, Imam Haryoko, Mehru Nisha, Sandeep Poddar","doi":"10.47836/mjmhs.19.s9.2","DOIUrl":"https://doi.org/10.47836/mjmhs.19.s9.2","url":null,"abstract":"Introduction: Hand Sanitizer is one of the health products that are in demand by the public, mainly for the duration of the contemporary COVID-19 pandemic. There are two kinds of Hand Sanitizers, made by chemicals and made by natural ingredients. There are many plants that contain active substances that can inhibit the growth of bacteria, namely saponins, flavonoids and essential oils and have a distinctive and sharp odor, one of which is the Betel Leaf plant (Piper betle L.) and lemongrass (Cymbopogon nardus L.). This study shows a comparison of bacterial colony numbers before and after the use of natural-based hand sanitizers a mixture of betel leaf decoction (Piper betle L.) and Lemongrass (Cymbopogon nardus L.). Methods: This study used an experimental- static group comparison design. The sampling technique in this study uses Accidental Sampling. The collected data was analyzed using the SPSS 16.0 application and were analyzed with the Unpaired T test. Results: Results were obtained, there has been a distinction withinside the wide variety of bacterial colonies before and after using disinfectant from mixed natural materials. Conclusion: It is concluded that there is a significant difference in Number of Bacteria Before and After Using a Hand sanitizer Made from Natural Ingredients A Mixture of Betel Leaf Decoction (Piper betle L.) And Lemongrass (Cymbopogon nardus L.).","PeriodicalId":40029,"journal":{"name":"Malaysian Journal of Medicine and Health Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135598907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determining the Efficacy of Albendazole Against STH Infection Among Orang Asli 测定阿苯达唑对猩猩STH感染的疗效

Malaysian Journal of Medicine and Health Sciences Pub Date : 2023-08-10 DOI: 10.47836/mjmhs.19.s9.28

Naim Khir, Mehru Nisha, Woei Yenn Tong, Fabian Davamani

{"title":"Determining the Efficacy of Albendazole Against STH Infection Among Orang Asli","authors":"Naim Khir, Mehru Nisha, Woei Yenn Tong, Fabian Davamani","doi":"10.47836/mjmhs.19.s9.28","DOIUrl":"https://doi.org/10.47836/mjmhs.19.s9.28","url":null,"abstract":"Introduction: According to WHO, soil-transmitted helminth (STH) infections could infect up to 90% of children from deprived communities with unhygiene and improper sanitation lifestyle. In West Malaysia, commonly the aboriginal people (Orang Asli) is prone to be infected with STH infections. Albendazole (400mg) is an anthelminthic drug that has been used for more than 30 years to cure STH infections. Hence, the main objective of this project was to evaluate the efficacy of single-dose albendazole (400mg) against STH infections among Orang Asli. Methods: Stool samples were collected in Hulu Langat, Semenyih, Malaysia. Who had not previously undergone deworming treatment. The parasites were concentrated by floatation technique (using the standard salt-sugar solution) to extract the amount of helminths’ egg before and after the albendazole treatment (400mg). Results: There were 44 participants after one-month post-treatment. The cure rate (CR) for Trichuris trichiura and Ascaris lumbricoides was 11.6% (n=5) and 35.0% (n=14), respectively. Moreover, there was no heavy infection for both STHs during the post-treatment of albendazole. Conclusion: The results indicated that the chosen anthelminthic showed higher cure rate efficacy in A.lumbricoides than T.trichiura. However, both species had decreased egg burden post-treatment with albendazole.","PeriodicalId":40029,"journal":{"name":"Malaysian Journal of Medicine and Health Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135598914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0