Comparison of the Number of Bacterial Colonies Before and After the Use of Natural Hand Sanitizers Mixed With Betel Leaf Decoction (Piper betle L) and Lemongrass (Cymbopogon nardus L)

Introduction: Hand Sanitizer is one of the health products that are in demand by the public, mainly for the duration of the contemporary COVID-19 pandemic. There are two kinds of Hand Sanitizers, made by chemicals and made by natural ingredients. There are many plants that contain active substances that can inhibit the growth of bacteria, namely saponins, flavonoids and essential oils and have a distinctive and sharp odor, one of which is the Betel Leaf plant (Piper betle L.) and lemongrass (Cymbopogon nardus L.). This study shows a comparison of bacterial colony numbers before and after the use of natural-based hand sanitizers a mixture of betel leaf decoction (Piper betle L.) and Lemongrass (Cymbopogon nardus L.). Methods: This study used an experimental- static group comparison design. The sampling technique in this study uses Accidental Sampling. The collected data was analyzed using the SPSS 16.0 application and were analyzed with the Unpaired T test. Results: Results were obtained, there has been a distinction withinside the wide variety of bacterial colonies before and after using disinfectant from mixed natural materials. Conclusion: It is concluded that there is a significant difference in Number of Bacteria Before and After Using a Hand sanitizer Made from Natural Ingredients A Mixture of Betel Leaf Decoction (Piper betle L.) And Lemongrass (Cymbopogon nardus L.).