Molekulyarnaya Biologiya最新文献_第10页

[IL-8 Links NF-κB and Wnt/β-Catenin Pathways in Persistent Inflammatory Response Induced by Chronic Helicobacter pylori Infection]. [IL-8在慢性幽门螺杆菌感染诱导的持续炎症反应中连接NF-κB和Wnt/β-儿茶素途径]。

Molekulyarnaya Biologiya Pub Date : 2023-07-01 DOI: 10.31857/S0026898423040134, EDN: QLUKEJ

L Lin, B Xie, J Shi, C M Zhou, J Yi, J Chen, J X He, H L Wei

{"title":"[IL-8 Links NF-κB and Wnt/β-Catenin Pathways in Persistent Inflammatory Response Induced by Chronic Helicobacter pylori Infection].","authors":"L Lin, B Xie, J Shi, C M Zhou, J Yi, J Chen, J X He, H L Wei","doi":"10.31857/S0026898423040134, EDN: QLUKEJ","DOIUrl":"10.31857/S0026898423040134, EDN: QLUKEJ","url":null,"abstract":"Helicobacter pylori (H. pylori) infection can cause persistent inflammatory response in human gastric mucosal epithelial cells, which may result in the occurrence of cancer. However, the underlying mechanism of carcinogenesis has not been elucidated yet. Herein, we established the models of chronic H. pylori infection in GES-1 cells and C57BL/6J mice. Interleukin 8 (IL-8) level was detected by ELISA. The expression of NF-κB p65, IL-8, Wnt2 and β-catenin mRNA and proteins was evaluated by real-time PCR, Western blotting, immunofluorescence staining, and immunohistochemistry. The infection of H. pylori in mice was evaluated by rapid urease test, H&E staining and Warthin-Starry silver staining. The morphological changes of gastric mucosa were observed by electron microscopy. Our results showed that in H. pylori infected gastric mucosal cells along with activation of NF-κB signaling pathway and increase of IL-8 level, the expression of Wnt2 was also increased significantly, which preliminarily indicates that IL-8 can positively regulate the expression of Wnt2. Studies in chronic H. pylori infected C57BL/6J mice models showed that there was an increased incidence of premalignant lesions in the gastric mucosa tissue. Through comparing changes of gastric mucosal cell ultrastructure and analyzing the relationship between NF-κB signaling pathway and Wnt2 expression, we found that H. pylori infection activated NF-κB signal pathways, and the massive release of IL-8 was positively correlated with the high expression of Wnt2 protein. Subsequently, the activated Wnt/β-catenin signal pathways may be involved in the malignant transformation of gastric mucosal cells. Collectively, H. pylori chronic infection may continuously lead to persistent inflammatory response: activate NF-κB pathway, promote IL-8 release and thereby activate Wnt/β-catenin pathway. IL-8 probably plays an important role of a linker in coupling these two signal pathways.","PeriodicalId":39818,"journal":{"name":"Molekulyarnaya Biologiya","volume":"57 4","pages":"713-716"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10277700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Universal Panel of Insertion/Deletion Polymorphisms and Biochip-Based Kit ChipID106 for Genetic Personal Identification]. [用于遗传个体鉴定的插入/缺失多态性和基于生物芯片的试剂盒ChipID106的通用面板]。

Molekulyarnaya Biologiya Pub Date : 2023-07-01 DOI: 10.31857/S0026898423040067, EDN: QLARZQ

D O Fesenko, I D Ivanovsky, P L Ivanov, E Yu Zemskova, S A Polyakov, O E Fesenko, M A Filippova, A S Zasedatelev

{"title":"[Universal Panel of Insertion/Deletion Polymorphisms and Biochip-Based Kit ChipID106 for Genetic Personal Identification].","authors":"D O Fesenko, I D Ivanovsky, P L Ivanov, E Yu Zemskova, S A Polyakov, O E Fesenko, M A Filippova, A S Zasedatelev","doi":"10.31857/S0026898423040067, EDN: QLARZQ","DOIUrl":"10.31857/S0026898423040067, EDN: QLARZQ","url":null,"abstract":"A panel of 106 insertion/deletion (InDel) polymorphisms and a method of their genotyping on biochips were proposed as a new approach to genetic personal identification. Short lengths and low mutation rates are basic properties of InDel markers, which thus have significant advantages over short tandem repeats (STRs) widely used in forensics. The allele frequency distributions of all known InDel polymorphisms were studied in the five largest world populations (European, East Asian, South Asian, African, and American). Markers were selected to meet the following criteria: the minor allele frequency (MAF) is higher than 0.30; the physical distance between markers is greater than 3 Mb; there are no polymorphisms, tandem repeats, and palindromes in the flanking sequences; the AT/GC ratio is close to 1. A panel of 106 polymorphisms was thus formed; the average MAF was estimated at 0.396 in the five populations. The method developed for panel genotyping included one-step multiplex PCR and subsequent hybridization on a biological microarray. The average amplicon length was 72 bp. A sample of 201 residents of Moscow and St. Petersburg was tested to determine the main characteristics of the panel: the random matching probability (MP) was 1.89x 10^(-43) and the combined probability of paternity exclusion (CPE) was 0.99999999063. The method provides an alternative to molecular genetic personal identification based on the STR length variations.","PeriodicalId":39818,"journal":{"name":"Molekulyarnaya Biologiya","volume":"57 4","pages":"632-646"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10295761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Random Integration Analysis of Recombinant Adeno-Associated Virus 6 Packaged in Sf9 Insect Cells]. [包装在Sf9昆虫细胞中的重组腺相关病毒6的随机整合分析]。

Molekulyarnaya Biologiya Pub Date : 2023-07-01 DOI: 10.31857/S0026898423040262, EDN: QMEZVF

M H Zhang, X M Liu, C Zhang

{"title":"[Random Integration Analysis of Recombinant Adeno-Associated Virus 6 Packaged in Sf9 Insect Cells].","authors":"M H Zhang, X M Liu, C Zhang","doi":"10.31857/S0026898423040262, EDN: QMEZVF","DOIUrl":"10.31857/S0026898423040262, EDN: QMEZVF","url":null,"abstract":"Recently, there have been growing concerns over the integration of recombinant adeno-associated virus (rAAV) used in gene therapy. Wild-type adeno-associated virus (AAV) site specifically integrates into AAVS1 site of human genome, while rAAV randomly integrates into host chromosomes at low frequencies. This research aims to study the random integration events of rAAV6-EGFP packaged in Sf9 insect cells. Baculo-Sf9 manufacturing platform has the advantages of high-density suspension culture of Sf9 insect cells and large-scale production of rAAV vectors. In this study, we used different doses of Baculo-Sf9 produced rAAV6-EGFP to transduce HEK293T cells and A549-implanted tumors in vitro and in vivo. Using flow cytometry and fluorescence microscopy, we studied their EGFP gene expression efficiencies and EGFP fluorescence intensities. Using inverse nested PCR and DNA sequencing, random integration sites of rAAV6-EGFP genome into human chromosomes were identified. In vitro results showed that gene expression efficiencies became stable after 20 days and random integration frequencies were 0.2-4.2%. Both in vitro and in vivo results indicated that random integration of Baculo-Sf9 rAAV6 was dose-dependent. Sequencing results showed two random integration sites, which were on human chromosomes 8 and 12. The findings suggest that we should use as low dose of rAAV vector as possible for safe gene therapy.","PeriodicalId":39818,"journal":{"name":"Molekulyarnaya Biologiya","volume":"57 4","pages":"709-712"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10277701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Alternative Mechanisms of Mutagenesis at mCpG Sites during Replication and Repair]. 【复制和修复过程中mCpG位点突变的替代机制】。

Molekulyarnaya Biologiya Pub Date : 2023-07-01 DOI: 10.31857/S0026898423040195, EDN: QLYEPA

E S Shilkin, D V Petrova, D O Zharkov, A V Makarova

引用次数: 0

[Interaction of DNA Methyltransferase Dnmt3a with Phosphorus Analogs of S-Adenosylmethionine and S-Adenosylhomocysteine]. [DNA甲基转移酶Dnmt3a与S-腺苷甲硫氨酸和S-腺苷同型半胱氨酸的磷类似物的相互作用]。

Molekulyarnaya Biologiya Pub Date : 2023-07-01 DOI: 10.31857/S0026898423040079, EDN: QLCJBH

V L Filonov, M A Khomutov, A V Sergeev, A L Khandazhinskaya, S N Kochetkov, E S Gromova, A R Khomutov

{"title":"[Interaction of DNA Methyltransferase Dnmt3a with Phosphorus Analogs of S-Adenosylmethionine and S-Adenosylhomocysteine].","authors":"V L Filonov, M A Khomutov, A V Sergeev, A L Khandazhinskaya, S N Kochetkov, E S Gromova, A R Khomutov","doi":"10.31857/S0026898423040079, EDN: QLCJBH","DOIUrl":"10.31857/S0026898423040079, EDN: QLCJBH","url":null,"abstract":"Enzymatic methyltransferase reactions are of crucial importance for cell metabolism. S-Adenosyl-L-methionine (AdoMet) is a main donor of the methyl group. DNA, RNA, proteins, and low-molecular-weight compounds are substrates of methyltransferases. In mammals, DNA methyltransferase Dnmt3a de novo methylates the C5 position of cytosine residues in CpG sequences in DNA. The methylation pattern is one of the factors that determine the epigenetic regulation of gene expression. Here, interactions with the catalytic domain of Dnmt3a was for the first time studied for phosphonous and phosphonic analogs of AdoMet and S-adenosyl-L-homocysteine (AdoHcy), in which the carboxyl group was substituted for respective phosphorus-containing group. These AdoMet analogs were shown to be substrates of Dnmt3a, and the methylation efficiency was only halved as compared with that of natural AdoMet. Both phosphorus-containing analogs of AdoHcy, which is a natural methyltransferase inhibitor, showed similar inhibitory activities toward Dnmt3a and were approximately four times less active than AdoHcy. The finding that the phosphonous and phosphonic analogs are similar in activity was quite unexpected because the geometry and charge of their phosphorus-containing groups differ substantially. The phosphorus-containing analogs of AdoMet and AdoHcy are discussed as promising tools for investigation of methyltransferases.","PeriodicalId":39818,"journal":{"name":"Molekulyarnaya Biologiya","volume":"57 4","pages":"717-725"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9974742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[MicroRNA Biogenesis in Cell Senescence Induced by Chronic Endoplasmic Reticulum Stress]. [慢性内质网应激诱导的细胞衰老中的微小RNA生物发生]。

Molekulyarnaya Biologiya Pub Date : 2023-07-01 DOI: 10.31857/S0026898423040250, EDN: QMCHZK

D M Zaichenko, A A Mikryukova, I R Astafeva, S G Malakho, A A Kubatiev, A A Moskovtsev

{"title":"[MicroRNA Biogenesis in Cell Senescence Induced by Chronic Endoplasmic Reticulum Stress].","authors":"D M Zaichenko, A A Mikryukova, I R Astafeva, S G Malakho, A A Kubatiev, A A Moskovtsev","doi":"10.31857/S0026898423040250, EDN: QMCHZK","DOIUrl":"10.31857/S0026898423040250, EDN: QMCHZK","url":null,"abstract":"MicroRNAs are small noncoding RNAs that regulate gene expression; stabilize the cell phenotype; and play an important role in cell differentiation, development, and apoptosis. A canonical microRNA biogenesis pathway includes several posttranscriptional steps of processing and transport and ends with cytoplasmic cleavage of pre-miRNA by type III ribonuclease DICER to form a mature duplex, which is included in RISC. MicroRNA biogenesis and role in cell stress are still poorly understood. Using flow cytometry and high-throughput analysis of gene expression, we have shown that chronic endoplasmic reticulum (ER) stress, which is associated with improper protein folding in the ER, induce a cellular senescence phenotype in fibroblast-like FRSN cells. While acute ER stress can reduce miRNA biogenesis, chronic stress does not cause a significant drop in global microRNA expression and is accompanied by only a slight decrease in DICER1 mRNA expression. Heterogeneity with respect to lysosomal β-galactosidase activity was found to increase in the cell population exposed to ER stress. We do not exclude induced cell heterogeneity regarding expression of components of the microRNA biogenesis pathway.","PeriodicalId":39818,"journal":{"name":"Molekulyarnaya Biologiya","volume":"57 4","pages":"671-686"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10295762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Inactivation of Ras1 in Fission Yeast Aggravates the Oxidative Stress Response Induced by Tert Butyl Hydroperoxide (tBHP)]. [裂解酵母中Ras1的失活加剧了由过氧化氢叔丁酯（tBHP）诱导的氧化应激反应]。

Molekulyarnaya Biologiya Pub Date : 2023-07-01 DOI: 10.31857/S0026898423040158, EDN: QLULLQ

N Masood, S Anjum, S Ahmed

{"title":"[Inactivation of Ras1 in Fission Yeast Aggravates the Oxidative Stress Response Induced by Tert Butyl Hydroperoxide (tBHP)].","authors":"N Masood, S Anjum, S Ahmed","doi":"10.31857/S0026898423040158, EDN: QLULLQ","DOIUrl":"10.31857/S0026898423040158, EDN: QLULLQ","url":null,"abstract":"Ras proteins are small GTPases and function as molecular switches to regulate cellular homeostasis. Ras-dependent signalling pathways regulate several essential processes such as cell cycle progression, growth, migration, apoptosis, and senescence. The dysregulation of Ras signaling pathway has been linked to several pathological outcomes. A potential role of RAS in regulating the redox signalling pathway has been established that includes the manipulation of ROS levels to provide a redox milieu that might be conducive to carcinogenesis. Reactive oxygen species (ROS) and mitochondrial impairment have been proposed as major factors affecting the physiology of cells and implicated in several pathologies. The present study was conducted to evaluate the role of Ras1, tert Butyl hydroperoxide (tBHP), and antimycin A in oxidative stress response in Schizosaccharomyces pombe cells. We observed decreased cell survival, higher levels of ROS, and mitochondrial dysfunctionality in ras1Δ cells and tBHP as well as respiratory inhibitor, antimycin A treated wild type cells. Furthermore, these defects were more profound in ras1Δ cells treated with tBHP or antimycin A. Additionally, Ras1 also has been shown to regulate the expression and activity of several antioxidant enzymes like glutathione peroxidase (GSH-Px), glutathione-S-transferase (GST), and catalase. Together, these results suggest the potential role of S. pombe Ras1 in mitigating oxidative stress response.","PeriodicalId":39818,"journal":{"name":"Molekulyarnaya Biologiya","volume":"57 4","pages":"689-691"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9974739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Mathematical Modeling of HIV Replicaton and the Response of the Interferon System]. 【HIV复制子和干扰素系统的反应的数学建模】。

Molekulyarnaya Biologiya Pub Date : 2023-07-01 DOI: 10.31857/S0026898423040080, EDN: QLNHZQ

I A Gainova, A E Soboleva, D S Grebennikov, G A Bocharov

引用次数: 0

[Apatinib Suppressed Macrophage-Mediated Malignant Behavior of Hepatocellular Carcinoma Cells via Modulation of VEGFR2/STAT3/PD-L1 Signaling]. [Apatinib通过调节VEGFR2/STAT3/PD-L1信号抑制巨噬细胞介导的肝细胞癌细胞的恶性行为]。

Molekulyarnaya Biologiya Pub Date : 2023-07-01 DOI: 10.31857/S0026898423040237, EDN: QMAQDY

T Yin, C B Fu, D D Wu, L Nie, H Chen, Y Wang

{"title":"[Apatinib Suppressed Macrophage-Mediated Malignant Behavior of Hepatocellular Carcinoma Cells via Modulation of VEGFR2/STAT3/PD-L1 Signaling].","authors":"T Yin, C B Fu, D D Wu, L Nie, H Chen, Y Wang","doi":"10.31857/S0026898423040237, EDN: QMAQDY","DOIUrl":"10.31857/S0026898423040237, EDN: QMAQDY","url":null,"abstract":"Hepatocellular carcinoma (HCC) is the most frequently diagnosed primary liver tumor worldwide. Tumor-associated macrophages (TAMs) usually have a similar phenotype to M2-like macrophages and can participate in tumor progression by secreting cytokines to suppress the immune response and activity of tumor-infiltrating lymphocytes. We investigated the role of M2 macrophages in HCC progression and explored the effects of vascular endothelial growth factor receptor 2 inhibitor-apatinib. As a cellular model of HCC, Hepb3 cell line was used. M2 macrophages were obtained by differentiation of THP-1 cells. The Transwell chamber was used to co-culture M2 macrophages and Hepb3 cells. CCK-8 and EdU assays were conducted to measure cell viability and proliferation capacity. Transwell migration assay was performed to estimate cellular metastatic potential. Cytokine expression levels were assessed by ELISA. Western blotting was used to characterize activation of the VEGFR2/STAT3/PD-L1 axis. It has been shown that co-culture with M2 macrophages increased viability, cytokine production, promoted proliferation, invasion, and migration of Hepb3 cells. The secretion of TGF-β1, IL-6, MMP-9, and VEGF was significantly increased after co-culture. In contrast apatinib suppressed M2 macrophage-induced proliferation, cell viability, invasion, and migration of Hepb3 cells. Moreover, apatinib markedly decreased expression levels of p-VEGFR2, p-STAT3, and PD-L1 in Hepb3 cells under the co-culture conditions. In conclusion, apatinib treatment can suppress TAMs-mediated malignant behavior of HCC cells via modulation of the VEGFR2/STAT3/PD-L1 signaling pathway.","PeriodicalId":39818,"journal":{"name":"Molekulyarnaya Biologiya","volume":"57 4","pages":"706-708"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10277705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Molecular and Cellular Aspects of the Endothelial-Mesenchymal Transition in Cardiovascular Diseases]. [心血管疾病中内皮-间充质转变的分子和细胞方面]。

Molekulyarnaya Biologiya Pub Date : 2023-07-01 DOI: 10.31857/S0026898423030138, EDN: CHRLDQ

E A Strelnikova, R E Kalinin, I A Suchkov, N V Korotkova, N D Mzhavanadze

{"title":"[Molecular and Cellular Aspects of the Endothelial-Mesenchymal Transition in Cardiovascular Diseases].","authors":"E A Strelnikova, R E Kalinin, I A Suchkov, N V Korotkova, N D Mzhavanadze","doi":"10.31857/S0026898423030138, EDN: CHRLDQ","DOIUrl":"10.31857/S0026898423030138, EDN: CHRLDQ","url":null,"abstract":"Endothelial cells (ECs), which form the inner surface of the blood vessels, contact the blood, withstand mechanical pressure, and demonstrate heterogeneous reactions to exogenous and endogenous stimuli. ECs have unique properties in accordance with their niches and play an important role in regulating vascular homeostasis. Endothelial cells may undergo a dynamic phenotypic switch in terms of its heterogeneity, which may lead to endothelial dysfunction and a number of associated pathologies. Endothelial-mesenchymal transition (EndMT) is one of the possible molecular and cellular mechanisms of this kind. EndMT is characterized by phenotypic changes in ECs through which endothelial cells acquire new properties, i.e., start producing mesenchymal markers such as alpha-SMA and vimentin, change morphology, and become able to migrate. EndMT is a complex biological process that can be induced by inflammation, hypoxia, or oxidative stress and be involved in pathogenesis of cardiovascular disease. This review describes the key markers, inhibitors, and inducers of endothelial-mesenchymal transition and overall state-of-the-art of EndMT in cardiovascular diseases.","PeriodicalId":39818,"journal":{"name":"Molekulyarnaya Biologiya","volume":"57 4","pages":"563-572"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10295759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0