Molekulyarnaya Biologiya最新文献_第10页

[The Oral Microbiome in the Development of Oral Cancer]. [口腔微生物组在口腔癌发展中的作用]。

Molekulyarnaya Biologiya Pub Date : 2024-03-01 DOI: 10.31857/S0026898424020041, EDN: NLORXN

E S Kolegova, A A Schegoleva, L A Kononova, E V Denisov

引用次数: 0

[Photochemical Processes of Cell DNA Damage by UV Radiation of Various Wavelengths: Biological Consequences]. [各种波长的紫外线辐射造成细胞 DNA 损伤的光化学过程：生物学后果]。

Molekulyarnaya Biologiya Pub Date : 2024-01-01 DOI: 10.31857/S0026898424010019, EDN: OHVEXS

G Ya Fraikin, N S Belenikina, A B Rubin

{"title":"[Photochemical Processes of Cell DNA Damage by UV Radiation of Various Wavelengths: Biological Consequences].","authors":"G Ya Fraikin, N S Belenikina, A B Rubin","doi":"10.31857/S0026898424010019, EDN: OHVEXS","DOIUrl":"https://doi.org/10.31857/S0026898424010019, EDN: OHVEXS","url":null,"abstract":"Photochemical reactions in cell DNA are induced in various organisms by solar UV radiation and may lead to a series of biological responses to DNA damage, including apoptosis, mutagenesis, and carcinogenesis. The chemical nature and the amount of DNA lesions depend on the wavelength of UV radiation. UV type B (UVB, 290-320 nm) causes two main lesions, cyclobutane pyrimidine dimers (CPDs) and, with a lower yield, pyrimidine (6-4) pyrimidone photoproducts (6-4PPs). Their formation is a result of direct UVB photon absorption by DNA bases. UV type A (UVA, 320-400 nm) induces only cyclobutane dimers, which most likely arise via triplet-triplet energy transfer (TTET) from cell chromophores to DNA thymine bases. UVA is much more effective than UVB in inducing sensitized oxidative DNA lesions, such as single-strand breaks and oxidized bases. Of the latter, 8-oxo-dihydroguanine (8-oxodG) is the most frequent, being produced in several oxidation processes. Many recent studies reported novel, more detailed information about the molecular mechanisms of the photochemical reactions that underlie the formation of various DNA lesions. The information is mostly summarized and analyzed in the review. Special attention is paid to the oxidation reactions that are initiated by reactive oxygen species (ROS) and radicals generated by potential endogenous photosensitizers, such as pterins, riboflavin, protoporphyrin IX, NADH, and melanin. The review discusses the role that specific DNA photoproducts play in genotoxic processes induced in living systems by UV radiation of various wavelengths, including human skin carcinogenesis.","PeriodicalId":39818,"journal":{"name":"Molekulyarnaya Biologiya","volume":"58 1","pages":"3-21"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141471413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[The Proteome of Extracellular Membrane Vesicles from Bacillus pumilus 3-19]. [枯草芽孢杆菌 3-19 胞外膜囊泡的蛋白质组]。

Molekulyarnaya Biologiya Pub Date : 2024-01-01 DOI: 10.31857/S0026898424010186, EDN: NRBQXZ

W Kurdy, P V Zelenikhin, G Yu Yakovleva, M N Sinyagina, A I Kolpakov, O N Ilinskaya

{"title":"[The Proteome of Extracellular Membrane Vesicles from Bacillus pumilus 3-19].","authors":"W Kurdy, P V Zelenikhin, G Yu Yakovleva, M N Sinyagina, A I Kolpakov, O N Ilinskaya","doi":"10.31857/S0026898424010186, EDN: NRBQXZ","DOIUrl":"https://doi.org/10.31857/S0026898424010186, EDN: NRBQXZ","url":null,"abstract":"Production of extracellular membrane vesicles plays an important role in communication in bacterial populations and in bacteria-host interactions. Vesicles as carriers of various regulatory and signaling molecules may be potentially used as disease biomarkers and promising therapeutic agents, including vaccine preparations. The composition of membrane vesicles has been deciphered for a limited number of Gram-negative and Gram-positive bacteria. In this work, for the first time, extracellular membrane vesicles of a streptomycin-resistant strain Bacillus pumilus 3-19, a producer of extracellular guanyl-preferring ribonuclease binase, are isolated, visualized, and characterized by their genome and proteome composition. It has been established that there is no genetic material in the vesicles and the spectrum of the proteins differs depending on the phosphate content in the culture medium of the strain. Vesicles from a phosphate-deficient medium carry 49 unique proteins in comparison with 101 from a medium with the high phosphate content. The two types of vesicles had 140 mutual proteins. Flagellar proteins, RNase J, which is the main enzyme of RNA degradosomes, phosphatases, peptidases, iron transporters, signal peptides, were identified in vesicles. Antibiotic resistance proteins and amyloid-like proteins whose genes are present in B. pumilus 3-19 cells are absent. Phosphate deficiency-induced binase was found only in vesicles from a phosphate-deficient medium.","PeriodicalId":39818,"journal":{"name":"Molekulyarnaya Biologiya","volume":"58 1","pages":"178-186"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141471424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Melatonin Enhances the Effect of ABT-737 in Acute Monocytic Leukemia THP-1 Cells]. [褪黑素能增强 ABT-737 对急性单核细胞白血病 THP-1 细胞的作用］

Molekulyarnaya Biologiya Pub Date : 2024-01-01 DOI: : 10.31857/S0026898424010136, EDN: NWVCMU

A I Lomovsky, Y L Baburina, R S Fadeev, M I Kobyakova, Ya V Lomovskaya, R R Krestinin, L D Sotnikova, O V Krestinina

{"title":"[Melatonin Enhances the Effect of ABT-737 in Acute Monocytic Leukemia THP-1 Cells].","authors":"A I Lomovsky, Y L Baburina, R S Fadeev, M I Kobyakova, Ya V Lomovskaya, R R Krestinin, L D Sotnikova, O V Krestinina","doi":": 10.31857/S0026898424010136, EDN: NWVCMU","DOIUrl":"https://doi.org/: 10.31857/S0026898424010136, EDN: NWVCMU","url":null,"abstract":"Melatonin (N-acetyl-5-methoxytryptamine, MEL) is a hormone synthesized by the pineal gland. Due to its oncostatic effect, it can be considered as an antitumor agent and used for combination therapy. ABT-737, a Bcl-2 inhibitor, promotes cell death after treatment with agents that induce pro-apoptotic signals. In the present study, the combined effect of MEL and ABT-737 on changes in proliferative and mitotic activity, mitochondrial membrane potential, intracellular production of reactive oxygen species (ROS), and cytosolic Ca^(2+) was studied. Moreover, changes in the expression of anti- and pro-apoptotic proteins (Bcl-2 and Bax), autophagy markers (LC3A/B (I, II)), endoplasmic reticulum stress markers (chaperones BIP and PDI, CHOP) were studied under these conditions. The effect of MEL together with ABT-737 led to an increase in the level of cytosolic Ca^(2+), intracellular production of ROS and a decrease in the membrane potential of mitochondria. The content of Bcl-2 increased, while the level of Bax decreased. Activation of CHOP stimulated autophagy and led to a decrease in the synthesis of chaperones BIP and PDI. It is assumed that melatonin can enhance the effect of other chemotherapeutic agents and can be used in the treatment of tumors.","PeriodicalId":39818,"journal":{"name":"Molekulyarnaya Biologiya","volume":"58 1","pages":"141-153"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141471412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[A Group of New Hypermethylated Long Non-Coding RNA Genes Associated with the Development and Progression of Breast Cancer]. [一组与乳腺癌的发生和发展有关的新的高甲基化长非编码 RNA 基因]。

Molekulyarnaya Biologiya Pub Date : 2024-01-01 DOI: 10.31857/S0026898424010085, EDN: ODZLJB

E A Filippova, V I Loginov, S S Lukina, A M Burdennyy, I V Pronina, T P Kazubskaya, E A Braga

{"title":"[A Group of New Hypermethylated Long Non-Coding RNA Genes Associated with the Development and Progression of Breast Cancer].","authors":"E A Filippova, V I Loginov, S S Lukina, A M Burdennyy, I V Pronina, T P Kazubskaya, E A Braga","doi":"10.31857/S0026898424010085, EDN: ODZLJB","DOIUrl":"https://doi.org/10.31857/S0026898424010085, EDN: ODZLJB","url":null,"abstract":"Breast cancer is the most common type of cancer among women. The study of the mechanisms of metastasis, the main cause of death from breast cancer, as well as the search for new markers for early diagnosis and prognosis of breast cancer, is an extremely topical issue. New perspectives in the diagnosis and treatment of breast cancer are opened by the mechanisms of gene regulation involving non-coding RNAs, in particular, long non-coding RNAs (lncRNAs). In this work, we analyzed the methylation levels of seven lncRNA genes (MEG3, SEMA3B-AS1, HAND2-AS1, KCNK15-AS1, ZNF667-AS1, MAGI2-AS3, and PLUT) by quantitative methyl-specific PCR on a set of 79 paired (tumor/normal) samples of breast cancer. Hypermethylation of all seven lncRNA genes was revealed, and hypermethylation of HAND2-AS1, KCNK15-AS1, MAGI2-AS3, and PLUT was detected in breast cancer for the first time. It was found that the level of meth ylation of the studied lncRNA genes correlated statistically significantly with the stage of the tumor process, the size of the tumor, and the presence of metastases in the lymph nodes. Thus, methylation of the seven studied lncRNA genes is associated with the development and progression of breast cancer, and these genes can be useful as potential markers in the diagnosis and prognosis of breast cancer.","PeriodicalId":39818,"journal":{"name":"Molekulyarnaya Biologiya","volume":"58 1","pages":"88-98"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141471409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[The Development of SpCas9 Variants with High Specificity and Efficiency Based on the HH Theory]. [基于 HH 理论开发高特异性和高效率的 SpCas9 变体]。

Molekulyarnaya Biologiya Pub Date : 2024-01-01 DOI: 10.31857/S0026898424010158, EDN: NTCLJI

G H Wang, C M Wang, X J Wu, T Chu, D W Huang, J Li

引用次数: 0

[Interaction of SENP6 with PINK1 Promotes Temozolomide Resistance in Neuroglioma Cells via Inducing the Mitophagy]. [SENP6与PINK1的相互作用通过诱导有丝分裂促进神经胶质瘤细胞对替莫唑胺的耐药性］

Molekulyarnaya Biologiya Pub Date : 2024-01-01

Y W Wang, K G Jia, H J Xing, Y Pan, C S Zeng, L Chen, Q J Su, W T Shen, J Chen, C Chen, Q Cao, Y Y Wang

{"title":"[Interaction of SENP6 with PINK1 Promotes Temozolomide Resistance in Neuroglioma Cells via Inducing the Mitophagy].","authors":"Y W Wang, K G Jia, H J Xing, Y Pan, C S Zeng, L Chen, Q J Su, W T Shen, J Chen, C Chen, Q Cao, Y Y Wang","doi":"","DOIUrl":"","url":null,"abstract":"Temozolomide resistance is a major cause of recurrence and poor prognosis in neuroglioma. Recently, growing evidence has suggested that mitophagy is involved in drug resistance in various tumor types. However, the role and molecular mechanisms of mitophagy in temozolomide resistance in glioma remain unclear. In this study, mitophagy levels in temozolomide-resistant and -sensitive cell lines were evaluated. The mechanisms underlying the regulation of mitophagy were explored through RNA sequencing, and the roles of differentially expressed genes in mitophagy and temozolomide resistance were investigated. We found that mitophagy promotes temozolomide resistance in glioma. Specifically, small ubiquitin-like modifier specific protease 6 (SENP6) promoted temozolomide resistance in glioma by inducing mitophagy. Protein-protein interactions between SENP6 and the mitophagy executive protein PTEN-induced kinase 1 (PINK1) resulted in a reduction in small ubiquitin-like modifier 2 (SUMO2)ylation of PINK1, thereby enhancing mitophagy. Our study demonstrates that by inducing mitophagy, the interaction of SENP6 with PINK1 promotes temozolomide resistance in glioblastoma. Therefore, targeting SENP6 or directly regulating mitophagy could be a potential and novel therapeutic target for reversing temozolomide resistance in glioma.","PeriodicalId":39818,"journal":{"name":"Molekulyarnaya Biologiya","volume":"57 6","pages":"126-129"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138809828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Prime-Editing Methods and pegRNA Design Programs]. [素材编辑方法和 pegRNA 设计程序]。

Molekulyarnaya Biologiya Pub Date : 2024-01-01 DOI: : 10.31857/S0026898424010023, EDN: OHRIQW

E V Mikhaylova, B R Kuluev, G A Gerashchenkov, D A Chemeris, R R Garafutdinov, A R Kuluev, An K Baymiev, Al K Baymiev, A V Chemeris

{"title":"[Prime-Editing Methods and pegRNA Design Programs].","authors":"E V Mikhaylova, B R Kuluev, G A Gerashchenkov, D A Chemeris, R R Garafutdinov, A R Kuluev, An K Baymiev, Al K Baymiev, A V Chemeris","doi":": 10.31857/S0026898424010023, EDN: OHRIQW","DOIUrl":"https://doi.org/: 10.31857/S0026898424010023, EDN: OHRIQW","url":null,"abstract":"It has been 10 years since CRISPR/Cas technology was applied to edit the genomes of various organisms. Its ability to produce a double-strand break in a DNA region specified by the researcher started a revolution in bioengineering. Later, the Base Editing (BE) method was developed. BE is performed via the formation of single-strand breaks by the mutant form of Cas nuclease (nickase), fused with deaminases and other enzymes. It can be used to promote A ↔ G and C ↔ T transitions, and a C → G transversion. Just over 3 years ago, a new Prime Editing (PE) variant of CRISPR/Cas was invented. Unlike BE, in PE the nickase is fused with reverse transcriptase, capable of building a new DNA chain using the pegRNA template. The pegRNA consists of an elongated guide RNA with an extra sequence at the 3'-end. Prime editing makes it possible to insert the desired mutations into this extra sequence and to carry out any substitutions and indels of bases without the use of special donor DNA. To date, a number of PE variants have been proposed; they are briefly considered in this review with an emphasis on prime editing of plant genomes. Some attention is also paid to pegRNA design programs, as well as evaluation of the efficiency of the editing. Such a variety of PE techniques is due to the opportunities of high-precision introduction of desired changes with a rather low frequency of off-target mutations in the genomes of various organisms. The relatively low efficiency of prime editing inspires researchers to offer new approaches. There is hope that further development of the technology will improve PE enough to take its rightful place among the genome targeting methods that are suitable for any organisms, and will have a positive impact on the agricultural sector, industrial biotechnologies, and medicine.","PeriodicalId":39818,"journal":{"name":"Molekulyarnaya Biologiya","volume":"58 1","pages":"22-39"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141471414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Structure and Evolution of DNA Transposons of the L31 Superfamily in Bivalves]. [双壳类 L31 超家族 DNA 转座子的结构与进化]。

Molekulyarnaya Biologiya Pub Date : 2024-01-01 DOI: 10.31857/S0026898424010051, EDN: OFVSWF

M V Puzakov, L V Puzakova

{"title":"[Structure and Evolution of DNA Transposons of the L31 Superfamily in Bivalves].","authors":"M V Puzakov, L V Puzakova","doi":"10.31857/S0026898424010051, EDN: OFVSWF","DOIUrl":"https://doi.org/10.31857/S0026898424010051, EDN: OFVSWF","url":null,"abstract":"The mobile genetic elements IS630/Tc 1/mariner (ITm) are widespread DNA transposons that make a significant contribution to the evolution of eukaryotic genomes. With the start of large-scale application of next-generation sequencing (NGS) technologies and the emergence of many new whole genome sequences of organisms in nucleotide sequence collections, the ITm elements have been identified in most taxa of the eukaryotic tree of life. Although ITm diversity has been studied in detail, new elements are still found, thus expanding the respective DNA transposon group and calling for review of its classification. Bivalve L31 elements were for the first time analyzed in detail to describe their structures, diversity, distribution, and phylogenetic position among the ITm elements. The L31 transposons were found to form an independent superfamily of an ancient origin within the ITm group. Rather high diversity was observed within the L31 clade; i.e., five phylogenetic clusters were identified. In mollusks, the L31 transposons have been detected only in the subclass Autobranchia and predominate in diversity and number in the infraclass Pteriomorphia. A protein encoded by open reading frame 2 (ORF2) was shown to be an integral structural component of almost all full-length L31 elements. The results provide for a better understanding of the evolution of particular ITm transposons. Further study of the L31 transposons in other taxa (cnidarians) and functional investigation of the ORF2 protein product will help to better understand the evolution of DNa transposons, the mechanisms of their horizontal transfer, and their contribution to eukaryotic biodiversity.","PeriodicalId":39818,"journal":{"name":"Molekulyarnaya Biologiya","volume":"58 1","pages":"54-72"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141471422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Upregulation of MHC I Antigen Processing Machinery Gene Expression in Breast Cancer Cells by Trichostatin A]. [Trichostatin A 对乳腺癌细胞中 MHC I 抗原加工机制基因表达的上调作用]。

Molekulyarnaya Biologiya Pub Date : 2024-01-01

A H Murtadha, N A Sharudin, I I M Azahar, A T Che Has, N F Mokhtar

{"title":"[Upregulation of MHC I Antigen Processing Machinery Gene Expression in Breast Cancer Cells by Trichostatin A].","authors":"A H Murtadha, N A Sharudin, I I M Azahar, A T Che Has, N F Mokhtar","doi":"","DOIUrl":"","url":null,"abstract":"Epigenetic alterations associated with cancer have been shown to facilitate tumorigenesis and promote metastasis. In the study of cancer metastasis, epigenetics has been revealed to play a crucial role in supporting tumour immune evasion. As a result, epigenetic drugs have been identified as potential agents to activate anti-tumour immune responses and reverse tumour immunologically tolerant states. Mounting evidence is showing aberrant expression of MHC class I antigen processing molecules in cancers and their upregulation as a potential indicator for anti-tumour immunity. In this study, we demonstrate that the epigenetic drug Trichostatin A (TSA), a histone deacetylase inhibitor, can restore MHC I antigen presentation machinery (MHC I APM) genes in human breast cancer cells (MCF-7). Treatment with TSA resulted in the upregulation of MHC I, B2M, and PSMB9 in MCF-7 monolayer cells, and MHC I, B2M, PSMB9, PSMB8, TAP1, and TAP2 in MCF-7 spheroid cells. Interestingly, treatment with TSA also increased CD274 expression in these cells and enhanced the invasion ability of the MCF-7 spheroid. This aggressive behaviour was confirmed by increased expression of metastatic-related genes, nNav1.5 and MMP1. In summary, although the restoration of MHCIAPM expression was achieved by TSA, the upregulation of metastatic genes and CD274 also enhanced the invasion ability of breast cancer cells. These findings suggest the need for careful consideration when utilizing epigenetic drugs for breast cancer therapy.","PeriodicalId":39818,"journal":{"name":"Molekulyarnaya Biologiya","volume":"57 6","pages":"121-125"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138810194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0