Journal of Smooth Muscle Research最新文献_第8页

Changes in renal vessels associated with long-term administration of angiotensin converting enzyme inhibitor in Zucker fatty rats. Zucker脂肪大鼠肾血管的变化与长期服用血管紧张素转换酶抑制剂有关。

Journal of Smooth Muscle Research Pub Date : 2017-01-01 DOI: 10.1540/jsmr.53.20

Kazushige Nakanishi, Yohko Nagai, Tatsuo Akimoto, Nobuaki Yamanaka

{"title":"Changes in renal vessels associated with long-term administration of angiotensin converting enzyme inhibitor in Zucker fatty rats.","authors":"Kazushige Nakanishi, Yohko Nagai, Tatsuo Akimoto, Nobuaki Yamanaka","doi":"10.1540/jsmr.53.20","DOIUrl":"https://doi.org/10.1540/jsmr.53.20","url":null,"abstract":"Background Recently, we showed that long-term angiotensin receptor blocker (ARB) administration induced unusual proliferative changes in smooth muscle cells (SMCs) of afferent arterioles of the kidneys of Zucker fatty rats (ZFRs). In this study, we investigated renal afferent arteriolar changes induced by the long-term administration of an angiotensin converting enzyme inhibitor (ACEI) in ZFRs. Materials and Methods Fourteen 6-week-old male ZFRs were divided into two groups (n=14): the ZFR+ACEI group (n=6) was fed a standard diet containing ACEI (Enalapril, 2 mg/kg/day), and the ZFR control group (n=8) for 12 weeks. Blood pressure and proteinuria were examined and morphological studies on kidneys were performed. Results Remarkable proliferative changes in the afferent arteriolar SMCs were frequently observed in the group given ACEI; (66.1 ± 12.9%) compared with the control group (1.77 ± 1.56%, P<0.001). Conclusions It was indicated that long-term ACEI administration induced unusual proliferative changes in SMCs in afferent arterioles of ZFRs. These changes could reduce intraglomerular pressure by narrowing the lumens of afferent arterioles, but they could cause irreversible damage to the arterioles.","PeriodicalId":39619,"journal":{"name":"Journal of Smooth Muscle Research","volume":"53 0","pages":"20-30"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1540/jsmr.53.20","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34783241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Role of prostatic interstitial cells in prostate motility. 前列腺间质细胞在前列腺运动中的作用。

Journal of Smooth Muscle Research Pub Date : 2017-01-01 DOI: 10.1540/jsmr.53.57

Richard J Lang, Hikaru Hashitani

{"title":"Role of prostatic interstitial cells in prostate motility.","authors":"Richard J Lang, Hikaru Hashitani","doi":"10.1540/jsmr.53.57","DOIUrl":"https://doi.org/10.1540/jsmr.53.57","url":null,"abstract":"The prostate is a gland whose secretions contribute to the seminal fluids ejaculated upon activation of autonomic sympathetic nerves. In elder males, the prostate undergoes an increase in stroma mass and myogenic tone, leading to benign prostatic hyperplasia that occludes the proximal urethra and the presentation of various lower urinary tract symptoms that decrease their quality of life. This review summarises the role of prostatic interstitial cells (PICs) in the generation of the spontaneous tone in the prostate. It presents current knowledge of the role of Ca2+ plays in PIC pacemaking, as well as the mechanisms by which this spontaneous activity triggers slow wave generation and stromal contraction. PICs display a small T-type Ca2+ current (ICaT) and a large L-type Ca2+ current (ICaL). In contrast to other interstitial cells in the urinary and gastrointestinal tracts, spontaneous Ca2+ signalling in PICs is uniquely dependent on Ca2+ influx through ICaL channels. A model of prostatic pacemaking is presented describing how ICaL can be triggered by an initial membrane depolarization evoked upon the selective opening of Ca2+-activated Cl- channels by Ca2+ flowing only through ICaT channels. The resulting current flow through ICaL results in release of Ca2+ from internal stores and the summation of Cl--selective spontaneous transient depolarizations (STDs) to form pacemaker potentials that propagate passively into the prostatic stroma to evoke regenerative action potentials and excitation-contraction coupling.","PeriodicalId":39619,"journal":{"name":"Journal of Smooth Muscle Research","volume":"53 0","pages":"57-72"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1540/jsmr.53.57","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35121297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Mechanisms underlying the pathogenesis of hyper-contractility of bronchial smooth muscle in allergic asthma. 过敏性哮喘支气管平滑肌过度收缩的发病机制。

Journal of Smooth Muscle Research Pub Date : 2017-01-01 DOI: 10.1540/jsmr.53.37

Hiroyasu Sakai, Wataru Suto, Yuki Kai, Yoshihiko Chiba

{"title":"Mechanisms underlying the pathogenesis of hyper-contractility of bronchial smooth muscle in allergic asthma.","authors":"Hiroyasu Sakai, Wataru Suto, Yuki Kai, Yoshihiko Chiba","doi":"10.1540/jsmr.53.37","DOIUrl":"https://doi.org/10.1540/jsmr.53.37","url":null,"abstract":"Airway hyperresponsiveness (AHR) and inflammation are key pathophysiological features of asthma. Enhanced contraction of bronchial smooth muscle (BSM) is one of the causes of the AHR. It is thus important for development of asthma therapy to understand the change in the contractile signaling of airway smooth muscle cells associated with the AHR. In addition to the Ca2+-mediated phosphorylation of myosin light chain (MLC), contractile agonists also enhance MLC phosphorylation level, Ca2+-independently, by inactivating MLC phosphatase (MLCP), called Ca2+ sensitization of contraction, in smooth muscle cells including airways. To date, involvements of RhoA/ROCKs and PKC/Ppp1r14a (also called as CPI-17) pathways in the Ca2+ sensitization have been identified. Our previous studies revealed that the agonist-induced Ca2+ sensitization of contraction is markedly augmented in BSMs of animal models of allergen-induced AHR. In BSMs of these animal models, the expression of RhoA and CPI-17 proteins were significantly increased, indicating that both the Ca2+ sensitizing pathways are augmented. Interestingly, incubation of BSM cells with asthma-associated cytokines, such as interleukin-13 (IL-13), IL-17, and tumor necrosis factor-α (TNF-α), caused up-regulations of RhoA and CPI-17 in BSM cells of naive animals and cultured human BSM cells. In addition to the transcription factors such as STAT6 and NF-κB activated by these inflammatory cytokines, an involvement of down-regulation of miR-133a, a microRNA that negatively regulates RhoA translation, has also been suggested in the IL-13- and IL-17-induced up-regulation of RhoA. Thus, the Ca2+ sensitizing pathways and the cytokine-mediated signaling including microRNAs in BSMs might be potential targets for treatment of allergic asthma, especially the AHR.","PeriodicalId":39619,"journal":{"name":"Journal of Smooth Muscle Research","volume":"53 0","pages":"37-47"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1540/jsmr.53.37","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34977270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 39

Diversity and plasticity in signaling pathways that regulate smooth muscle responsiveness: Paradigms and paradoxes for the myosin phosphatase, the master regulator of smooth muscle contraction. 调节平滑肌反应性的信号通路的多样性和可塑性:平滑肌收缩的主要调节剂肌球蛋白磷酸酶的范式和悖论。

Journal of Smooth Muscle Research Pub Date : 2017-01-01 DOI: 10.1540/jsmr.53.1

Masumi Eto, Toshio Kitazawa

{"title":"Diversity and plasticity in signaling pathways that regulate smooth muscle responsiveness: Paradigms and paradoxes for the myosin phosphatase, the master regulator of smooth muscle contraction.","authors":"Masumi Eto, Toshio Kitazawa","doi":"10.1540/jsmr.53.1","DOIUrl":"https://doi.org/10.1540/jsmr.53.1","url":null,"abstract":"A hallmark of smooth muscle cells is their ability to adapt their functions to meet temporal and chronic fluctuations in their demands. These functions include force development and growth. Understanding the mechanisms underlying the functional plasticity of smooth muscles, the major constituent of organ walls, is fundamental to elucidating pathophysiological rationales of failures of organ functions. Also, the knowledge is expected to facilitate devising innovative strategies that more precisely monitor and normalize organ functions by targeting individual smooth muscles. Evidence has established a current paradigm that the myosin light chain phosphatase (MLCP) is a master regulator of smooth muscle responsiveness to stimuli. Cellular MLCP activity is negatively and positively regulated in response to G-protein activation and cAMP/cGMP production, respectively, through the MYPT1 regulatory subunit and an endogenous inhibitor protein named CPI-17. In this article we review the outcomes from two decade of research on the CPI-17 signaling and discuss emerging paradoxes in the view of signaling pathways regulating smooth muscle functions through MLCP.","PeriodicalId":39619,"journal":{"name":"Journal of Smooth Muscle Research","volume":"53 0","pages":"1-19"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1540/jsmr.53.1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34783242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 28

Role of mast cells in bronchial contraction in nonallergic obstructive lung pathology. 肥大细胞在非过敏性阻塞性肺病理支气管收缩中的作用。

Journal of Smooth Muscle Research Pub Date : 2017-01-01 DOI: 10.1540/jsmr.53.90

Nataliya A Kuzubova, Elena S Lebedeva, Olga N Titova, Anatoliy N Fedin, Ivetta V Dvorakovskaya

{"title":"Role of mast cells in bronchial contraction in nonallergic obstructive lung pathology.","authors":"Nataliya A Kuzubova, Elena S Lebedeva, Olga N Titova, Anatoliy N Fedin, Ivetta V Dvorakovskaya","doi":"10.1540/jsmr.53.90","DOIUrl":"https://doi.org/10.1540/jsmr.53.90","url":null,"abstract":"The role of mast cells in contractile bronchial smooth muscle activity has been evaluated in a model of chronic obstructive pulmonary disease induced in rats that were intermittently exposed to nitrogen dioxide (NO2) for 60 days. Starting from the 31st day, one group of rats inhaled sodium cromoglycate before exposure to NO2 to stabilize mast cell membranes. The second group (control) was not treated. Isometric smooth muscle contraction was analysed in isolated bronchial samples in response to nerve and smooth muscle stimulation. Histological analysis revealed large numbers of mast cells in lung tissue of COPD model rats. The inhibition of mast cell degranulation by sodium cromoglycate prevented the development of nerve-stimulated bronchial smooth muscle hyperactivity in COPD model rats. Histamine or adenosine-induced hyperactivity on nerve stimulation was also inhibited by sodium cromoglycate in bronchial smooth muscle in both control and COPD model rats. This suggests that the mechanism of contractile activity enhancement of bronchial wall smooth muscle cells may be mediated through the activation of resident mast cells transmembrane adenosine receptors resulting in their partial degranulation, with the released histamine acting upon histamine H1-receptors which trigger reflex pathways via intramural ganglion neurons.","PeriodicalId":39619,"journal":{"name":"Journal of Smooth Muscle Research","volume":"53 0","pages":"90-99"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1540/jsmr.53.90","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35373018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Synthetic smooth muscle in the outer blood plexus of the rhinarium skin of Lemur catta L. 狐猴鼻皮肤外血丛合成平滑肌。

Journal of Smooth Muscle Research Pub Date : 2017-01-01 DOI: 10.1540/jsmr.53.31

Rolf Elofsson, Ronald H H Kröger

引用次数: 1

Gastric emptying after artificial ulceration in rats: differences due to the site of the ulcer and the effects of prokinetic drugs. 大鼠人工溃疡后胃排空:溃疡部位及促胃动力药物作用的差异。

Journal of Smooth Muscle Research Pub Date : 2017-01-01 DOI: 10.1540/jsmr.53.48

Masayuki Uchida, Orie Kobayashi, Kimiko Shimizu

{"title":"Gastric emptying after artificial ulceration in rats: differences due to the site of the ulcer and the effects of prokinetic drugs.","authors":"Masayuki Uchida, Orie Kobayashi, Kimiko Shimizu","doi":"10.1540/jsmr.53.48","DOIUrl":"https://doi.org/10.1540/jsmr.53.48","url":null,"abstract":"Background This study aimed to evaluate the effects of the position of an acetic acid-induced gastric ulcer and the effects of prokinetic drugs on gastric emptying. Materials and Methods Male Sprague-Dawley rats were used in this study. Acetic acid ulcers were induced either in the region between the fundus and pylorus on the anterior wall of the stomach or in the glandular region on the greater curvature of the stomach to determine whether there were regional differences in the effect of the ulcers. Gastric emptying was evaluated with a breath test using [1-13C] acetic acid. In addition, the effects of the prokinetic drugs, metoclopramide and mosapride, on gastric emptying were also evaluated. Results Acetic acid induced ulcers in the region between the fundus and pylorus on the anterior wall of the stomach significantly delayed gastric emptying as compared with control rats, but not the acetic acid induced ulcers in the glandular region on the greater curvature of the stomach. Metoclopramide and mosapride did not improve the delayed gastric emptying even at doses that enhanced gastric emptying in normal rats. Conclusion These findings show that gastric emptying is influenced by the position of the ulcer and the region between the fundus and pylorus on the anterior wall plays an important role in gastric emptying. Moreover, it was found that metoclopramide and mosapride do not improve the delayed gastric emptying caused by acetic acid ulcers induced on the anterior wall in the region between the fundus and pylorus.","PeriodicalId":39619,"journal":{"name":"Journal of Smooth Muscle Research","volume":"53 0","pages":"48-56"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1540/jsmr.53.48","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35121296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Deterioration of epithelium mediated mechanisms in diabetic-antigen sensitized airways of guinea pigs 豚鼠糖尿病抗原致敏气道中上皮介导的恶化机制

Journal of Smooth Muscle Research Pub Date : 2016-12-27 DOI: 10.1540/jsmr.52.93

S. Bano, Omanwar Swati, Muralidhar Kambadur, F. Mohammad

{"title":"Deterioration of epithelium mediated mechanisms in diabetic-antigen sensitized airways of guinea pigs","authors":"S. Bano, Omanwar Swati, Muralidhar Kambadur, F. Mohammad","doi":"10.1540/jsmr.52.93","DOIUrl":"https://doi.org/10.1540/jsmr.52.93","url":null,"abstract":"Background The onset of diabetes causes disruption of respiratory epithelial mediators. The present study investigates whether diabetes modifies the epithelium mediated bronchial responses in hyper-reactive airway smooth muscle (ASM) primarily through nitric oxide (NO), cyclooxygenase (COX), and epithelium derived hyperpolarizing factor (EpDHF) pathways. Methods Experimental model of guinea pigs having hyper-reactive airways with or without diabetes were developed. The responses of tracheal rings to cumulative concentrations of acetylcholine (ACh) and isoproterenol (IP) in the presence and absence of epithelium and before and after incubation with NO, K+ATP and COX inhibitors, N-(ω)-Nitro-L-arginine methyl ester (L-NAME; 100 µM), glybenclamide (10 µM) and indomethacin (100 µM) were assessed. Results In diabetic guinea pigs with hyper-reactive airways, a decrease in ACh induced bronchoconstriction was observed after epithelium removal and after incubation with L-NAME/indomethacin, suggesting damage to NO/COX pathways. Hyper-reactivity did not alter the response of trachea to ACh but affected the response to IP which was further reduced in hyper-reactive animals with diabetes. The ASM response to IP after glybenclamide treatment did not alter in hyper-reactive guinea pigs and diabetic guinea pigs with hyper-reactive airways, suggesting damage to the EpDHF pathway. Treatment with indomethacin reduced IP response in the hyper-reactive model, and did not produce any change in diabetic model with hyper-reactive airways, indicating further disruption of the COX pathway. Conclusion EpDHF pathway is damaged in hyper-reactive guinea pigs and in diabetic guinea pigs with hyper-reactive airways. Diabetes further aggravates the NO and COX mediated pathways in diabetic guinea pigs with hyper-reactive airways.","PeriodicalId":39619,"journal":{"name":"Journal of Smooth Muscle Research","volume":"52 1","pages":"93 - 104"},"PeriodicalIF":0.0,"publicationDate":"2016-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1540/jsmr.52.93","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67067469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Significant contribution of TRPC6 channel-mediated Ca2+ influx to the pathogenesis of Crohn's disease fibrotic stenosis TRPC6通道介导的Ca2+内流在克罗恩病纤维化狭窄发病机制中的重要贡献

Journal of Smooth Muscle Research Pub Date : 2016-11-03 DOI: 10.1540/jsmr.52.78

L. Kurahara, K. Hiraishi, M. Sumiyoshi, Mayumi Doi, Yaopeng Hu, K. Aoyagi, Yuwen Jian, R. Inoue

{"title":"Significant contribution of TRPC6 channel-mediated Ca2+ influx to the pathogenesis of Crohn's disease fibrotic stenosis","authors":"L. Kurahara, K. Hiraishi, M. Sumiyoshi, Mayumi Doi, Yaopeng Hu, K. Aoyagi, Yuwen Jian, R. Inoue","doi":"10.1540/jsmr.52.78","DOIUrl":"https://doi.org/10.1540/jsmr.52.78","url":null,"abstract":"Intestinal fibrosis is an intractable complication of Crohn's disease (CD), and, when occurring excessively, causes severe intestinal obstruction that often necessitates surgical resection. The fibrosis is characterized by an imbalance in the turnover of extracellular matrix (ECM) components, where intestinal fibroblasts/myofibroblasts play active roles in ECM production, fibrogenesis and tissue remodeling, which eventually leads to the formation of stenotic lesions. There is however a great paucity of knowledge about how intestinal fibrosis initiates and progresses, which hampers the development of effective pharmacotherapies against CD. Recently, we explored the potential implications of transient receptor potential (TRP) channels in the pathogenesis of intestinal fibrosis, since they are known to act as cellular stress sensors/transducers affecting intracellular Ca2+ homeostasis/dynamics, and are involved in a broad spectrum of cell pathophysiology including inflammation and tissue remodeling. In this review, we will place a particular emphasis on the intestinal fibroblast/myofibroblast TRPC6 channel to discuss its modulatory effects on fibrotic responses and therapeutic potential for anti-fibrotic treatment against CD-related stenosis.","PeriodicalId":39619,"journal":{"name":"Journal of Smooth Muscle Research","volume":"52 1","pages":"78 - 92"},"PeriodicalIF":0.0,"publicationDate":"2016-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1540/jsmr.52.78","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67067455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

MYPT1 isoforms expressed in HEK293T cells are differentially phosphorylated after GTPγS treatment gtp - γ s处理后，HEK293T细胞中表达的MYPT1亚型发生了差异磷酸化

Journal of Smooth Muscle Research Pub Date : 2016-10-07 DOI: 10.1540/jsmr.52.66

Simon Lin, F. Brozovich

引用次数: 3