Journal of Smooth Muscle Research最新文献_第6页

Accelerating effects of blebbistatin on relaxation process of cell membrane permeabilized trachea and taenia cecum from guinea pig. blebbistatin对豚鼠气管及盲带绦虫细胞膜通透性松弛过程的加速作用。

Journal of Smooth Muscle Research Pub Date : 2020-01-01 DOI: 10.1540/jsmr.56.19

Satoko Mihashi, Yukisato Ishida, Masaru Watanabe

引用次数: 0

Physiological function and molecular composition of ATP-sensitive K⁺ channels in human gastric smooth muscle. 人胃平滑肌atp敏感K+通道的生理功能及分子组成。

Journal of Smooth Muscle Research Pub Date : 2020-01-01 DOI: 10.1540/jsmr.56.29

Sang Eok Lee, Dae Hoon Kim, Seung Myeung Son, Song-Yi Choi, Ra Young You, Chan Hyung Kim, Woong Choi, Hun Sik Kim, Yung Ji Lim, Ji Young Han, Hyun Woo Kim, In Jun Yang, Wen-Xie Xu, Sang Jin Lee, Young Chul Kim, Hyo-Yung Yun

{"title":"Physiological function and molecular composition of ATP-sensitive K+ channels in human gastric smooth muscle.","authors":"Sang Eok Lee, Dae Hoon Kim, Seung Myeung Son, Song-Yi Choi, Ra Young You, Chan Hyung Kim, Woong Choi, Hun Sik Kim, Yung Ji Lim, Ji Young Han, Hyun Woo Kim, In Jun Yang, Wen-Xie Xu, Sang Jin Lee, Young Chul Kim, Hyo-Yung Yun","doi":"10.1540/jsmr.56.29","DOIUrl":"https://doi.org/10.1540/jsmr.56.29","url":null,"abstract":"Gastric motility is controlled by slow waves. In general, the activation of the ATP-sensitive K+ (KATP) channels in the smooth muscle opposes the membrane excitability and produces relaxation. Since metabolic inhibition and/or diabetes mellitus are accompanied by dysfunctions of gastric smooth muscle, we examined the possible roles of KATP channels in human gastric motility. We used human gastric corpus and antrum smooth muscle preparations and recorded the mechanical activities with a conventional contractile measuring system. We also identified the subunits of the KATP channels using Western blot. Pinacidil (10 μM), a KATP channel opener, suppressed contractions to 30% (basal tone to -0.2 g) of the control. The inhibitory effect of pinacidil on contraction was reversed to 59% of the control by glibenclamide (20 μM), a KATP channel blocker. The relaxation by pinacidil was not affected by a pretreatment with L-arginine methyl ester, tetraethylammonium, or 4-aminopyridine. Pinacidil also inhibited the acetylcholine (ACh)-induced tonic and phasic contractions in a glibenclamide-sensitive manner (42% and 6% of the control, respectively). Other KATP channel openers such as diazoxide, cromakalim and nicorandil also inhibited the spontaneous and ACh-induced contractions. Calcitonin gene-related peptide (CGRP), a gastric neuropeptide, induced muscle relaxation by the activation of KATP channels in human gastric smooth muscle. Finally, we have found with Western blot studies, that human gastric smooth muscle expressed KATP channels which were composed of Kir 6.2 and SUR2B subunits.","PeriodicalId":39619,"journal":{"name":"Journal of Smooth Muscle Research","volume":"56 0","pages":"29-45"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1540/jsmr.56.29","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38081389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Diagnosis and treatments for oropharyngeal dysphagia: effects of capsaicin evaluated by newly developed ultrasonographic method. 口咽吞咽困难的诊断与治疗:新发展的超声方法评价辣椒素的作用。

Journal of Smooth Muscle Research Pub Date : 2020-01-01 DOI: 10.1540/jsmr.56.46

Rui Nakato, Noriaki Manabe, Kozo Hanayama, Hiroaki Kusunoki, Jiro Hata, Ken Haruma

引用次数: 5

The inhibitory effect of somatostatin on gastric motility in Suncus murinus. 生长抑素对鼠子胃运动的抑制作用。

Journal of Smooth Muscle Research Pub Date : 2020-01-01 DOI: 10.1540/jsmr.56.69

Haruka Sekiya, Naho Yokota, Shota Takemi, Keiji Nakayama, Hiroki Okada, Takafumi Sakai, Ichiro Sakata

{"title":"The inhibitory effect of somatostatin on gastric motility in Suncus murinus.","authors":"Haruka Sekiya, Naho Yokota, Shota Takemi, Keiji Nakayama, Hiroki Okada, Takafumi Sakai, Ichiro Sakata","doi":"10.1540/jsmr.56.69","DOIUrl":"https://doi.org/10.1540/jsmr.56.69","url":null,"abstract":"Gastric contractions show two specific patterns in many species, migrating motor contractions (MMC) and postprandial contractions (PPCs), that occur in the fasted and fed states, respectively. In this study, we examined the role of somatostatin (SST) in gastric motility both in vivo and in vitro using the Asian house shrew (Suncus murinus). We performed in vivo recordings of gastric motility and in vitro organ bath experiments using S. murinus, which was recently established as a small laboratory animal for use in tests of gastrointestinal motility. SST (1.65 µg kg-1 min-1) was intravenously administered during phase II of MMC and PPCs. Next, the effect of SST on motilin-induced gastric contractions at phase I of MMC was measured. Cyclosomatostatin (CSST), an SST receptor antagonist, was administered at the peak of phase III of MMC. In addition, the effect of SST (10-11-10-9 M) on motilin-induced gastric contractions was evaluated using an organ bath experiment in vitro. In conscious, free-moving S. murinus, the administration of SST decreased the occurrence of the spontaneous phase II of MMC and PPCs. Pretreatment with SST and octreotide suppressed the induction of motilin-induced gastric contractions both in vivo and in vitro. Administration of CSST before the peak of spontaneous phase III contractions had no effect on gastric contractions. Endogenous SST is not involved in the regulation of gastric MMC and PPCs, but exogenous SST suppresses spontaneous gastric contractions. Thus, SST would be good for treating abnormal gastrointestinal motility disorders.","PeriodicalId":39619,"journal":{"name":"Journal of Smooth Muscle Research","volume":"56 0","pages":"69-81"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ae/4f/jsmr-56-069.PMC7817339.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38761996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Muscle cells, nerves, fibroblasts and vessels in the detrusor of the rat urinary bladder 大鼠膀胱逼尿肌中的肌肉细胞、神经、成纤维细胞和血管

Journal of Smooth Muscle Research Pub Date : 2019-11-09 DOI: 10.1540/jsmr.55.34

G. Gabella

{"title":"Muscle cells, nerves, fibroblasts and vessels in the detrusor of the rat urinary bladder","authors":"G. Gabella","doi":"10.1540/jsmr.55.34","DOIUrl":"https://doi.org/10.1540/jsmr.55.34","url":null,"abstract":"All the cells of rat detrusor muscle fall into one of five ultrastructural types: muscle cells, fibroblasts, axons and glia, and vascular cells (endothelial cells and pericytes). The tissue is ~79% cellular and 21% non-cellular. Muscle cells occupy 72%, nerves ~4% (1/3 axons, 2/3 glia), and fibroblast >3% of space. Muscle cells (up to 6 µm across and ~600 µm long, packed to almost 100,000 per mm2) have surface-to-volume ratio of 2.4 µm2/µm3 ~93% of cell volume is contractile apparatus, 3.1% mitochondria and 2.5% nucleus. Cell profiles are irregular but sectional area decreases regularly towards either end of the cell. Muscle cells are gathered into bundles (the mechanical units of detrusor), variable in length and size, but of constant width. The musculature is highly compact (without fascia or capsule) with smooth outer surfaces and extensive association and adhesion between its cells. Among many types of intercellular contact and junction, digitations are very common, each muscle cell issuing minute finger-like processes that abut on adjacent cells. Sealed apposition are wide areas of specialized contact, possibly forming a chamber between two muscle cells, distinct from the extracellular space at large (stromal space). The innervation is very dense, virtually all intramuscular axons being varicose (including afferent ones). There are identifiable neuro-muscular junctions on each muscle cell, often several junctions on a single cell. There are also unattached terminals. Fibroblasts (involved in the production of collagen), ~1% of the total number of cells, do not make specialized contacts.","PeriodicalId":39619,"journal":{"name":"Journal of Smooth Muscle Research","volume":"55 1","pages":"34 - 67"},"PeriodicalIF":0.0,"publicationDate":"2019-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1540/jsmr.55.34","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45449328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

The protective effect of endothelin receptor antagonists against surgically induced impairment of gastrointestinal motility in rats 内皮素受体拮抗剂对大鼠胃肠运动损伤的保护作用

Journal of Smooth Muscle Research Pub Date : 2019-09-19 DOI: 10.1540/jsmr.55.23

Hanna Ługowska-Umer, A. Umer, K. Kuziemski, Łukasz Sein-Anand, R. Korolkiewicz

{"title":"The protective effect of endothelin receptor antagonists against surgically induced impairment of gastrointestinal motility in rats","authors":"Hanna Ługowska-Umer, A. Umer, K. Kuziemski, Łukasz Sein-Anand, R. Korolkiewicz","doi":"10.1540/jsmr.55.23","DOIUrl":"https://doi.org/10.1540/jsmr.55.23","url":null,"abstract":"Endothelin (ET) receptor antagonists: BQ-123 (ETA), BQ-788 (ETB), tezosentan (dual ET receptor antagonist) protect against the development of postoperative ileus (POI) evoked by ischemia-reperfusion (I/R). The current experiments explored whether ET antagonists prevent the occurrence of POI evoked by surgical gut manipulation. Intestinal transit was assessed by measuring the rate of dye migration subsequent to skin incision (SI), laparotomy (L), or laparotomy and surgical gut handling (L+M) in diethyl ether anaesthesized rats (E). Experimental animals were randomly sub-divided into two groups depending on the time of recovery following surgery: viz. either 2 or 24 h (early or late phase POI). E and SI did not affect the gastrointestinal (GI) transit. In contrast, L and L+M significantly reduced GI motility in comparison to untreated group (UN). Tezosentan (10 mg/kg), BQ-123 and BQ-788 (1 mg/kg) protected against development of L+M evoked inhibition of intestinal motility in the course of late phase, but not early phase POI. Furthermore, tezosentan alleviated the decrease in the contractile response of the longitudinal jejunal smooth muscle strips to carbachol in vitro induced by L+M. The serum ET(1–21) concentration was not increased in either the early or the late phase POI groups after surgery compared to control animals. This study indicates that delay in the intestinal transit in late phase of surgically induced POI involves an ET-dependent mechanism.","PeriodicalId":39619,"journal":{"name":"Journal of Smooth Muscle Research","volume":"55 1","pages":"23 - 33"},"PeriodicalIF":0.0,"publicationDate":"2019-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1540/jsmr.55.23","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47838889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Sustained exposure to prostaglandin D₂ augments the contraction induced by acetylcholine via a DP₁ receptor-mediated activation of p38 in bronchial smooth muscle of naive mice. 持续暴露于前列腺素D2可通过DP1受体介导的支气管平滑肌p38的激活增强乙酰胆碱诱导的收缩。

Journal of Smooth Muscle Research Pub Date : 2019-01-01 DOI: 10.1540/jsmr.55.1

Wataru Suto, Hiroyasu Sakai, Yoshihiko Chiba

{"title":"Sustained exposure to prostaglandin D2 augments the contraction induced by acetylcholine via a DP1 receptor-mediated activation of p38 in bronchial smooth muscle of naive mice.","authors":"Wataru Suto, Hiroyasu Sakai, Yoshihiko Chiba","doi":"10.1540/jsmr.55.1","DOIUrl":"https://doi.org/10.1540/jsmr.55.1","url":null,"abstract":"Prostaglandin D2 (PGD2), one of the key lipid mediators of allergic airway inflammation, is increased in the airways of asthmatics. However, the role of PGD2 in the pathogenesis of asthma is not fully understood. In the present study, effects of PGD2 on smooth muscle contractility of the airways were determined to elucidate its role in the development of airway hyperresponsiveness (AHR). In a murine model of allergic asthma, antigen challenge to the sensitized animals caused a sustained increase in PGD2 levels in bronchoalveolar lavage (BAL) fluids, indicating that smooth muscle cells of the airways are continually exposed to PGD2 after the antigen exposure. In bronchial smooth muscles (BSMs) isolated from naive mice, a prolonged incubation with PGD2 (10-5 M, for 24 h) induced an augmentation of contraction induced by acetylcholine (ACh): the ACh concentration-response curve was significantly shifted upward by the 24-h incubation with PGD2. Application of PGD2 caused phosphorylation of ERK1/2 and p38 in cultured BSM cells: both of the PGD2-induced events were abolished by laropiprant (a DP1 receptor antagonist) but not by fevipiprant (a DP2 receptor antagonist). In addition, the BSM hyperresponsiveness to ACh induced by the 24-h incubation with PGD2 was significantly inhibited by co-incubation with SB203580 (a p38 inhibitor), whereas U0126 (a ERK1/2 inhibitor) had no effect on it. These findings suggest that prolonged exposure to PGD2 causes the BSM hyperresponsiveness via the DP1 receptor-mediated activation of p38. A sustained increase in PGD2 in the airways might be a cause of the AHR in allergic asthmatics.","PeriodicalId":39619,"journal":{"name":"Journal of Smooth Muscle Research","volume":"55 0","pages":"1-13"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1540/jsmr.55.1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37095819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Recent advances in intestinal smooth muscle research: from muscle strips and single cells, via ICC networks to whole organ physiology and assessment of human gut motor dysfunction. 肠道平滑肌研究的最新进展：从肌条和单细胞，到 ICC 网络，再到整个器官生理学和人体肠道运动功能障碍评估。

Journal of Smooth Muscle Research Pub Date : 2019-01-01 DOI: 10.1540/jsmr.55.68

Jan D Huizinga

{"title":"Recent advances in intestinal smooth muscle research: from muscle strips and single cells, via ICC networks to whole organ physiology and assessment of human gut motor dysfunction.","authors":"Jan D Huizinga","doi":"10.1540/jsmr.55.68","DOIUrl":"10.1540/jsmr.55.68","url":null,"abstract":"Gastrointestinal smooth muscle research has evolved from studies on muscle strips to spatiotemporal mapping of whole organ motor and electrical activities. Decades of research on single muscle cells and small sections of isolated musculature from animal models has given us the groundwork for interpretation of human in vivo studies. Human gut motility studies have dramatically improved by high-resolution manometry and high-resolution electrophysiology. The details that emerge from spatiotemporal mapping of high-resolution data are now of such quality that hypotheses can be generated as to the physiology (in healthy subjects) and pathophysiology (in patients) of gastrointestinal (dys) motility. Such interpretation demands understanding of the musculature as a super-network of excitable cells (neurons, smooth muscle cells, other accessory cells) and oscillatory cells (the pacemaker interstitial cells of Cajal), for which mathematical modeling becomes essential. The developing deeper understanding of gastrointestinal motility will bring us soon to a level of precision in diagnosis of dysfunction that is far beyond what is currently available.","PeriodicalId":39619,"journal":{"name":"Journal of Smooth Muscle Research","volume":"55 0","pages":"68-80"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/dd/20/jsmr-55-068.PMC6962316.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37559065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibitory effects of rubratoxin A, a potent inhibitor of protein phosphatase 2, on the Ca²⁺-dependent contraction of skinned carotid artery from guinea pig. 一种有效的蛋白磷酸酶2抑制剂rubratoxin A对豚鼠皮肤颈动脉Ca2+依赖性收缩的抑制作用。

Journal of Smooth Muscle Research Pub Date : 2019-01-01 DOI: 10.1540/jsmr.55.14

Yasuyuki Naraki, Masaru Watanabe, Kosuke Takeya

引用次数: 0

Implications of immune-inflammatory responses in smooth muscle dysfunction and disease. 免疫炎症反应在平滑肌功能障碍和疾病中的意义。

Journal of Smooth Muscle Research Pub Date : 2019-01-01 DOI: 10.1540/jsmr.55.81

Fumitake Usui-Kawanishi, Masafumi Takahashi, Hiroyasu Sakai, Wataru Suto, Yuki Kai, Yoshihiko Chiba, Keizo Hiraishi, Lin Hai Kurahara, Masatoshi Hori, Ryuji Inoue

{"title":"Implications of immune-inflammatory responses in smooth muscle dysfunction and disease.","authors":"Fumitake Usui-Kawanishi, Masafumi Takahashi, Hiroyasu Sakai, Wataru Suto, Yuki Kai, Yoshihiko Chiba, Keizo Hiraishi, Lin Hai Kurahara, Masatoshi Hori, Ryuji Inoue","doi":"10.1540/jsmr.55.81","DOIUrl":"https://doi.org/10.1540/jsmr.55.81","url":null,"abstract":"In the past few decades, solid evidence has been accumulated for the pivotal significance of immunoinflammatory processes in the initiation, progression, and exacerbation of many diseases and disorders. This groundbreaking view came from original works by Ross who first described that excessive inflammatory-fibroproliferative response to various forms of insult to the endothelium and smooth muscle of the artery wall is essential for the pathogenesis of atherosclerosis (Ross, Nature 1993; 362(6423): 801-9). It is now widely recognized that both innate and adaptive immune reactions are avidly involved in the inflammation-related remodeling of many tissues and organs. When this state persists, irreversible fibrogenic changes would occur often culminating in fatal insufficiencies of many vital parenchymal organs such as liver, lung, heart, kidney and intestines. Thus, inflammatory diseases are becoming the common life-threatening risk for and urgent concern about the public health in developed countries (Wynn et al., Nature Medicine 2012; 18(7): 1028-40). Considering this timeliness, we organized a special symposium entitled \"Implications of immune/inflammatory responses in smooth muscle dysfunction and disease\" in the 58th annual meeting of the Japan Society of Smooth Muscle Research. This symposium report will provide detailed synopses of topics presented in this symposium; (1) the role of inflammasome in atherosclerosis and abdominal aortic aneurysms by Fumitake Usui-Kawanishi and Masafumi Takahashi; (2) Mechanisms underlying the pathogenesis of hyper-contractility of bronchial smooth muscle in allergic asthma by Hiroyasu Sakai, Wataru Suto, Yuki Kai and Yoshihiko Chiba; (3) Vascular remodeling in pulmonary arterial hypertension by Keizo Hiraishi, Lin Hai Kurahara and Ryuji Inoue.","PeriodicalId":39619,"journal":{"name":"Journal of Smooth Muscle Research","volume":"55 0","pages":"81-107"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1540/jsmr.55.81","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37613602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0