Polski Merkuriusz Lekarski最新文献

{"title":"Expect the unexpected: bezoar-caused gastric perforation in the 19-year-old patient, after traffic accident.","authors":"Jerzy Janusz Miłow, Justyna Joźwiak","doi":"10.36740/Merkur202401119","DOIUrl":"10.36740/Merkur202401119","url":null,"abstract":"<p><p>A 19-year-old female involved in a traffic accident presented to the Emergency Room (ER) with no trauma-related symptoms but a palpable mass in the epigastrium. Imaging revealed a massive trichobezoar causing gastric perforation. Urgent laparotomy was performed, and a 1.5-kilogram bezoar was removed, along with repairing coexisting gastric ulcers. The patient had a history of trichophagia, suggesting a psychiatric association. This case highlights the potential of trichobezoars to cause gastric perforation, even in patients admitted for unrelated reasons. CT-scan proves effective in diagnosing such cases. While a traffic accident might be a plausible cause, the presence of a bezoar can elevate the risk of complications. Psychiatric evaluation is recommended when trichophagia is identified. The study underscores the need for vigilance in unexpected scenarios, demonstrating the importance of multidisciplinary approaches in managing such cases.</p>","PeriodicalId":39518,"journal":{"name":"Polski Merkuriusz Lekarski","volume":"52 1","pages":"128-131"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140190204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The severity and outcome of Covid-19 depend on the various activities of the renin-angiotensin-aldosterone system, level of the opioid growth factor, [met5]-enkephalin and pre-existing comorbidities.","authors":"Irina A Komarevtseva, Kateryna V Balabanova, Anna Yu Ihnatova, Vitaliy N Komarevtsev, Andriy I Burachyk, Oleksandr A Zhurba, Yuri A Chernykh","doi":"10.36740/Merkur202405113","DOIUrl":"https://doi.org/10.36740/Merkur202405113","url":null,"abstract":"<p><strong>Objective: </strong>Aim: The aim of the study was evaluation of the methionine-enkephalin in patients with severe COVID-19 with various activities of the renin-aldosterone system in comparison with COVID-19 patients with pre-existing comorbidities (renal cell cancer, critical limb ischemia) and adverse pregnancy outcomes..</p><p><strong>Patients and methods: </strong>Materials and Methods: To test our hypothesis, this case-control study consisted of 20 healthy donors (control group); 49 patients with a positive diagnosis of COVID-19 according to PCR analysis; 15 patients with a positive diagnosis of COVID-19 in combination with renal cell cancer; 29 patients with a positive diagnosis of COVID-19 in combination with critical limb ischemia, 10 pregnant womens with COVID-19. Methionine-enkephalin, plasma renin activity, plasma aldosterone was measured by radioimmunoassay. Statistical and graphical analyses were done using Statistica 7.0 StatSoft software and using MedCalc.</p><p><strong>Results: </strong>Results: As our results have shown, hyperreninemia against the background of hypoaldosteronism and inhibition of enkephalinergic activity is fatal for COVID-19 patients. In COVID-19 patients with renal cell carcinoma, met-enkephalin, aldsterone, and plasma renin activity also showed a high predictive value for mortality. Whereas in patients with critical limb ischemia the strongest predictor biomarkers of mortality were only renin plasma activity. In conditions of physiological hyperreninemia during pregnancy, met-enkephalin acts as a biomarker for preterm birth, and in pregnant patients with COVID-19, plasma renin activity acts as such a biomarker.</p><p><strong>Conclusion: </strong>Conclusions: We found that in different categories of patients with COVID-19, the severity and outcome depend on the different activity of the renin-angiotensinaldosterone and enkephalinergic systems.</p>","PeriodicalId":39518,"journal":{"name":"Polski Merkuriusz Lekarski","volume":"52 5","pages":"562-567"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142847714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamics of future law enforcement officers' psychophysical state indicators in the course of their diverse training sessions on motor activity.","authors":"Ivan M Okhrimenko, Victoria A Shtykh, Tetiana M Kostenko, Ivan I Lukasevich, Tetiana М Grebeniunk","doi":"10.36740/Merkur202406110","DOIUrl":"https://doi.org/10.36740/Merkur202406110","url":null,"abstract":"<p><strong>Objective: </strong>Aim: The aim is to study the impact of training sessions in various service-applied sports on the dynamics of cadets' psychophysical state indicators in the course of their instruction at a higher educational institution with specific learning environment..</p><p><strong>Patients and methods: </strong>Materials and Methods: The research was conducted in 2019-2023. The research involved 155 male cadets aged 17-23 who were divided into those who were additionally engaged in various service-applied sports during their instruction (n = 115) and those who, in addition to compulsory physical training, did not additionally engage in sports (group d, n = 40). Cadets' physical state was assessed by the indicators of their body weight, body length, age, heart rate, blood pressure and physical state index. The mental state was studied by the indicators of cadets' emotional resilience.</p><p><strong>Results: </strong>Results: It has been found that most indicators of physical state and emotional resilience of cadets who were engaged in sports significantly improved in the course of their instruction. The most pronounced effect on the indicators of physical state was found in the group of cadets who were engaged in all-around sports, and on the indicators of emotional resilience - in the group of cadets who were engaged in martial arts.</p><p><strong>Conclusion: </strong>Conclusions: The research showed that additional training in service-applied sports has a more effective impact on the psychophysical state of future law enforcement officers compared to traditional physical training sessions. Improving the psychophysical state of cadets in the training process will help to improve their future law enforcement activities.</p>","PeriodicalId":39518,"journal":{"name":"Polski Merkuriusz Lekarski","volume":"52 6","pages":"685-690"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143417044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MiNa scale: Assessing mindfulness in nature and its impact on the mental health, wellbeing and holistic development of young children.","authors":"Agathi Argyriadi, Alexandros Argyriadis, Emmanuel Kopanakis, Panagiotis Koutras, Isabel Vieira, Olga Drakopoulou, Konstantinos Louvaris, Zoi Mammi, Dimitra Katsarou, Panagiotis Paoullis","doi":"10.36740/Merkur202406104","DOIUrl":"https://doi.org/10.36740/Merkur202406104","url":null,"abstract":"<p><strong>Objective: </strong>. Aim: The aim of this study was to design, validate, and assess the effectiveness of the MiNa scale in measuring mindfulness and its impact on young students' mental health and holistic development, with a focus on diverse educational needs.</p><p><strong>Patients and methods: </strong>Materials and Methods: This study utilized a mixed-methods approach to validate the MiNa Questionnaire. Exploratory Factor Analysis (EFA) and Confirmatory Factor Analysis (CFA) were conducted on a sample of 224 students from Greece and Cyprus, who completed the questionnaire. Psychometric properties, including reliability and validity, were also assessed using standard statistical methods.</p><p><strong>Results: </strong>Results: EFA revealed a three-factor structure explaining 53% of the variance, with strong factor loadings and reliability (Cronbach's α > 0.7). CFA further confirmed the model fit, with RMSEA = 0.03, CFI = 0.97, and SRMR = 0.08. The results indicated significant improvements in students that attended mindfulness interventional programs, especially on their emotional regulation, and cognitive focus. It is important to note that positive results were predominantly observed among the sample of students characterized by vulnerability.</p><p><strong>Conclusion: </strong>Conclusions: The MiNa Questionnaire has proven to be a reliable and valid tool for assessing mindfulness in students from diverse educational backgrounds. Its adaptability for students with disabilities, combined with its cultural inclusivity, makes it a valuable resource for educators seeking to implement mindfulness programs in varied educational contexts.</p>","PeriodicalId":39518,"journal":{"name":"Polski Merkuriusz Lekarski","volume":"52 6","pages":"638-646"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143417093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Problematic issues in developing public health policy based on data usage.","authors":"Tetiana S Gruzieva","doi":"10.36740/Merkur202406111","DOIUrl":"https://doi.org/10.36740/Merkur202406111","url":null,"abstract":"<p><strong>Objective: </strong>Aim: To identify the opportunities, approaches, challenges, and needs of professionals at disease control and prevention centers in the process of using data for developing public health policy..</p><p><strong>Patients and methods: </strong>Materials and Methods: The program provides for sociological surveys of professionals from disease control and prevention centers regarding the opportunities, needs, and challenges in using data to develop public health policy. The research materials included scientific publications, national-level regulatory legal acts, WHO program documents and sociological research questionnaires. The study utilized bibliosemantic, sociological, medical-statistical methods, and content analysis.</p><p><strong>Results: </strong>Results: A range of issues was identified regarding the use of data for substantiating and developing public health policy. A survey of professionals from disease control and prevention centers indicated that they encounter a lack of data on social and mental health ([rate ± standard error] 19,7±3,5 per 100 respondents); work with at-risk populations (15,0±3,2); socioeconomic determinants (14,2±3,1); occupational diseases and labor protection (13,4±3,0); and non-communicable diseases and behavioral risk factors (11,0±2,8). Every sixth to tenth respondent reported challenges in obtaining and analyzing data. A high need (74,8±3,8 per 100 respondents) was identified for professional development in data usage for policy substantiation, along with preferred formats.Priority measures for improving data use in public health policy development were identified.</p><p><strong>Conclusion: </strong>Conclusions: The identified issues in data usage for public health policy development, as well as the needs of professionals at disease control and prevention centers concerning data collection and analysis, and the priority measures for improvement, should be considered.</p>","PeriodicalId":39518,"journal":{"name":"Polski Merkuriusz Lekarski","volume":"52 6","pages":"691-697"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143417099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimal tactics for surgical revascularisation in diabetic angiopathy of the lower limbs.","authors":"Manat Zhakubayev, Tolegen Zh Yegemberdiyev, Alimir I Tolegen, Talgat Tajibayev","doi":"10.36740/Merkur202404105","DOIUrl":"https://doi.org/10.36740/Merkur202404105","url":null,"abstract":"<p><strong>Objective: </strong>Aim: This study aims to compare the efficacy of conservative treatment methods versus advanced surgical interventions, including revascularising automyelotransplantation and stem cell therapy, in improving vascular patency and the quality of life in patients with diabetic angiopathy.</p><p><strong>Patients and methods: </strong>Materials and Methods: The research analyzed 68 patients with angiopathies under medical supervision from January 2007 to December 2017 at the National Scientific Center of Surgery named after A.N. Syzganov. Participants, aged 4 to 49, were divided into two groups based on angiographic blood flow characteristics: one with accelerated and another with delayed blood flow. A comprehensive participant selection process was implemented to ensure a representative sample. Sensitivity analysis was conducted to validate the findings' robustness.</p><p><strong>Results: </strong>Results: The main group demonstrated notable success in limb salvage, with 90.9% avoiding high limb amputation post-revascularising automyelotransplantation. Moreover, 16.7% of patients experienced healing of trophic ulcers and toe necrosis. The use of stem cells from adipose tissue and fetal tissue progenitor cells showed promising results in reducing pain and increasing pain-free walking distance, alongside the formation of collateral vascular networks.</p><p><strong>Conclusion: </strong>Conclusions: The study concludes that advanced surgical interventions and stem cell therapies significantly enhance treatment outcomes in patients with diabetic angiopathy compared to conventional conservative treatments. These findings highlight the potential of personalized and innovative approaches in managing vascular complications associated with diabetes, offering new avenues for reducing disability and improving patient quality of life. Future research should focus on further refining these therapeutic strategies and exploring their integration into clinical practice.</p>","PeriodicalId":39518,"journal":{"name":"Polski Merkuriusz Lekarski","volume":"52 4","pages":"415-420"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142366853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maintaining the mental health of Ukrainians in time of war: searching for a mechanism to provide a comprehensive system of psychosocial support and mental health awareness.","authors":"Viktor I Vus, Mariana V Shkoliar, Serhii Proskuriakov, Evangelos Fradelos","doi":"10.36740/Merkur202403116","DOIUrl":"10.36740/Merkur202403116","url":null,"abstract":"<p><strong>Objective: </strong>Aim: To substantiate the possibilities of developing a comprehensive system of psychosocial support for Ukrainians during and after the war through thedevelopment of an integrated model of psychosocial service provision in the community, which promotes cross-sectoral interaction and expands the possibilities of integrating and scaling up multiple levels of mental health interventions.</p><p><strong>Patients and methods: </strong>Materials and Methods: The article is based on the use of bibliosemantic analysis of scientific works on the topic of mental health and mental health during the war.</p><p><strong>Conclusion: </strong>Conclusions: It is emphasised that in developing a comprehensive system of psychosocial support, it is necessary to: ensure accessibility of services for those in need; integrate services into the general health and social protection system; use a multisectoral approach, involving various organisations, institutions and professionals; train professionals to work in war conditions; develop and implement psychoeducation and psychological support programmes; monitor and evaluate the effectiveness of programmes. Particular attention is paid to psychoeducation as a technology that can be used at different levels of psychological intervention by both mental health professionals and other specialists involved in the provision of social services in communities. The spread of the impact of psychoeducational programmes will contribute to the development of community resilience in the face of social and psychological risks provoked by the war. A mechanism for scaling up the capabilities of the Technical Working Group on Mental Health and Psychosocial Support (MHPSS TWG), an association of leading international and Ukrainian NGOs specialising in mental health, established in Ukraine with the support of the WHO and the Ministry of Health of Ukraine, is proposed. The idea of creating a network of Resilience Centres in communities with the aim of forming a comprehensive system of psychosocial support at the state and community levels is substantiated.</p>","PeriodicalId":39518,"journal":{"name":"Polski Merkuriusz Lekarski","volume":"52 3","pages":"373-379"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141617351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Special at-rich sequence-binding protein 2 and its role in healing of the experimental mandible bone tissue defect filling with a synthetic bone graft material and electrical stimulation impact.","authors":"Agil N Huseynov, Vladislav A Malanchuk, Mykhailo S Myroshnychenko, Nataliia V Kapustnyk, Liliia P Sukharieva, Larisa I Selivanova","doi":"10.36740/Merkur202404101","DOIUrl":"https://doi.org/10.36740/Merkur202404101","url":null,"abstract":"<p><strong>Objective: </strong>Aim: The purpose of the study was to identify the role of SATB2 in healing of the experimental mandible bone tissue defect filling with a synthetic bone graft material and electrical stimulation impact.</p><p><strong>Patients and methods: </strong>Materials and Methods: An experiment was carried out on 48 mature male rats of the WAG population, which were divided into 4 groups. Each group included 12 experimental animals. Group 1 included rats that were modeled with a perforated defect of the lower jaw body. Group 2 included animals that were modeled with a perforated defect similar to group 1. In animals, a microdevice for electrical action was implanted subcutaneously in the neck area on the side of the simulated bone defect. The negative electrode connected to the negative pole of the battery was in contact with the bone defect. The battery and electrode were insulated with plastic heat shrink material. Group 3 included rats that were modeled with a perforated defect similar to previous groups, the cavity of which was filled with synthetic bone graft \"Biomin GT\" (RAPID, Ukraine). Group 4 included animals that were modeled with a perforated defect similar to groups 1-3, the cavity of which was filled with synthetic bone graft \"Biomin GT\" (RAPID, Ukraine). The simulation of electrical stimulation was the same as in group 2. The material for the morphological study was a fragment of the body of the lower jaw from the zone of the perforated defect. Immunohistochemical study was performed using rabbit anti-human SATB2 monoclonal antibody.</p><p><strong>Results: </strong>Results: In the regenerate filling the defect in the bone tissue of the lower jaw of rats, there was an increase in SATB2 expression under conditions of electrical stimulation; filling the defect with a synthetic bone graft material; simultaneous filling the defect with a synthetic bone graft material and electrical stimulation. The most pronounced expression of SATB2 was observed under conditions of simultaneous filling the defect with a synthetic bone graft material and electrical stimulation; minimally expressed - in conditions of filling the defect with a synthetic bone graft material; moderately expressed - under conditions of electrical stimulation. In the regenerate, in cases of all treatment methods, SATB2 was expressed by immune cells, fibroblastic differon cells, osteoblasts, and in case of electrical stimulation, also by adipocytes, vascular pericytes and endothelial cells, epidermis.</p><p><strong>Conclusion: </strong>Conclusions: The activation of SATB2 expression identified by the authors is one of the mechanisms for stimulating reparative osteogenesis under the conditions of electrical stimulation; filling the defect with a synthetic bone graft material; simultaneous filling the defect with a synthetic bone graft material and electrical stimulation.</p>","PeriodicalId":39518,"journal":{"name":"Polski Merkuriusz Lekarski","volume":"52 4","pages":"385-391"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142366854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression features of T-lymphocytes, B-lymphocytes and macrophages in the post-traumatic regenerate of the mandible rats under conditions of filling a bone defect with hydroxyapatite-containing osteotropic material and thymalin injecting the surrounding soft tissues.","authors":"Andrii A Boiko, Vladislav A Malanchuk, Mykhailo S Myroshnychenko, Olena V Markovska, Anton S Shapkin, Dmytro I Marakushyn","doi":"10.36740/Merkur202402105","DOIUrl":"https://doi.org/10.36740/Merkur202402105","url":null,"abstract":"<p><strong>Objective: </strong>Aim: The purpose of the study was to determine the features of the expression of T-lymphocytes, B-lymphocytes, macrophages in the post-traumatic regenerate of the mandible rats under conditions of filling a bone defect with hydroxyapatite-containing osteotropic material and thymalin injecting the surrounding soft tissues.</p><p><strong>Patients and methods: </strong>Materials and Methods: An experiment was conducted on 48 mature rats of the WAG population weighing 160-180 grams. Four groups were formed. Group 1 included 12 rats with a simulated holey defect in the lower jaw. Group 2 included 12 rats with a simulated holey defect in the lower jaw followed by its closure with hydroxyapatite-containing osteotropic material (bone graft \"Biomin GT\"). Group 3 included 12 rats with a simulated holey defect in the lower jaw with injecting the surrounding soft tissues with thymalin. Group 4 included 12 rats with a simulated holey defect in the lower jaw followed by its closure with hydroxyapatite-containing osteotropic material (bone graft \"Biomin GT\") and injecting the surrounding soft tissues with thymalin. The material for the morphological study was a fragment of the lower jaw from the area of the simulated holey defect. An immunohistochemical study was aperformed using monoclonal antibodies to CD68, CD20, CD163, CD86, CD3.</p><p><strong>Results: </strong>Results: A comprehensive experimental and morphological study conducted by the authors revealed that thymalin injection of the soft tissues surrounding the bone defect of the lower jaw, filled with hydroxyapatite-containing osteotropic material \"Biomin GT\", stimulates local immune reactions in the post-traumatic regenerate, which is manifested, firstly, by an increase in the number T-lymphocytes on the 3rd day of the experiment and their increase up to the 28th day; secondly, by increasing the number of B-lymphocytes on the 14th day of the experiment with their further increase up to the 28th day; thirdly, by increasing the number of macrophages on the 3rd day of the experiment and their growth up to the 28th day; fourth, changes in macrophages phenotypes (decrease in the number of M1-macrophages and increase in the number of M2-macrophages).</p><p><strong>Conclusion: </strong>Conclusions: Stimulation of local immune reactions in the post-traumatic regenerate can be one of the mechanisms that activate reparative osteogenesis in the lower jaw of rats under the conditions of filling bone defects with hydroxyapatite-containing osteotropic material \"Biomin GT\" and thymalin injecting the surrounding soft tissues.</p>","PeriodicalId":39518,"journal":{"name":"Polski Merkuriusz Lekarski","volume":"52 2","pages":"171-177"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140866691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histological and immunohistochemical characteristic of the gingival stroma in the portion of the third molars in children of various age.","authors":"Oksana I Godovanets, Igor S Davydenko, Taras I Muryniuk, Larysa Ya Fedoniuk","doi":"10.36740/Merkur202402103","DOIUrl":"https://doi.org/10.36740/Merkur202402103","url":null,"abstract":"<p><strong>Objective: </strong>Aim: To evaluate the state of the gingival stromal elements in the portion of the third molars requiring extraction of these teeth due to orthodontic indications considering the stage of tooth germ formation.</p><p><strong>Patients and methods: </strong>Materials and Methods: The surgery to extract third molars due to orthodontic indications was performed on 95 children aged 11 to 18 years. The three groups of observation were isolated according to clinical-radiological signs: І (n=30) - children aged 11-13 years; ІІ (n=35) - children aged 13-16 years, and ІІІ (n=30) - children aged 16-18 years. During surgery, the samples of gums were taken from the adjacent areas for examination. The samples were fixed, dehydrated, paraffinized for further histological processing. Immunohistochemical methods were used according to the protocols supplied by a producer. In particular, by means of immunohistochemical method, Ki-67, CD-34 antigens and vimentin with primary antibodies against them were determined. The primary antibodies were visualized by the polymeric visualization system with diaminobenzidine giving a brown color to the places of location of the antigens examined. The data obtained were statistically processed.</p><p><strong>Results: </strong>Results: The results of the study showed that specific gravity of the vascular bed in the gingival papillary layer of children was the most variable. It ranges from (12,7±0,09) % at the stage of \"D\" root formation to (54,8±0,17) % at the \"H\" stage. Lower concentrations of CD-34 antigens and vimentin are found in the endotheliocytes of children aged 13-16 and 16-18 years, compared to the children aged 11-13 years (p<0,05). No changes were found in the specific volume of the blood vessels, CD-34 antigens and vimentin in the reticular gingival layer of children from the groups of observation.</p><p><strong>Conclusion: </strong>Conclusions: Therefore, the conducted histological and immunohistochemical study of the connective gingival tissues in the portion of the third molars in children enables to draw a conclusion that in the process of formation of the root of this tooth a number of changes occur in the gingival stroma. They include an increase of the blood flow volume in the papillary gingival layer on the background of a decreased concentration of CD-34 genes and vimentin, a longer stage of development of the third molar root. The specific volume of the islets of neoangiogenesis of the papillary gingival layer is the largest in children aged 13-16 years.</p>","PeriodicalId":39518,"journal":{"name":"Polski Merkuriusz Lekarski","volume":"52 2","pages":"153-160"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140866698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}