Indian Journal of Agricultural Biochemistry最新文献

Production, purification and In vitro anticancer efficiency of L-asparaginase obtained from bacterial isolate of black gram rhizospheric soil sample of J&K 从美国黑革兰根际土壤样品中分离得到l -天冬酰胺酶的制备、纯化及体外抗癌效果

Indian Journal of Agricultural Biochemistry Pub Date : 2025-01-01 DOI: 10.5958/0974-4479.2025.00012.2

Sweeta Manhas, Vikas Sharma, Shilpa Raina, Asha Chaubey

{"title":"Production, purification and In vitro anticancer efficiency of L-asparaginase obtained from bacterial isolate of black gram rhizospheric soil sample of J&K","authors":"Sweeta Manhas, Vikas Sharma, Shilpa Raina, Asha Chaubey","doi":"10.5958/0974-4479.2025.00012.2","DOIUrl":"https://doi.org/10.5958/0974-4479.2025.00012.2","url":null,"abstract":"AbstractAsparaginase is broadly found in plants, animals and microbes, but not in humans. Varied microorganism sources such as yeast, fungi and bacteria are generally used for L-asparaginase production as it is trivial to extract the enzyme from plants and animals. However, anaphylaxis and hypersensitivity reactions associated with asparaginase obtained from microbial sources exhibits low specificity towards asparagine. Therefore, inhibition and toxicityactivity ofbacterial Lasparaginasecenters towards improved adequacy of the enzyme by investigating other microbial sources to purify and produce L-asparaginase of high specificity to asparagine. This may be of great help in overcoming hypersensitive reactions caused by known sources which could further notably upgrade therapeutic index of asparaginase therapy. Rhizospheric soil sample of black gram was collected from Rajouri region of J&K followed by isolation and screening of pure culture with remarkable activity. Purification was carried out by anion exchange using a mono-Q column attached to Biologic- Duoflow FPLC system. In vitro cytotoxic potential of purified protein was carried out against four different human cancer cell lines namely MDA-MB-231(breast), MIAPACA(pancreas), MDA-MB-435(melanoma), A-498(renal) and the enzyme showed growth inhibition of breast and pancreatic human cancer cell lines.","PeriodicalId":39477,"journal":{"name":"Indian Journal of Agricultural Biochemistry","volume":"38 1","pages":"74-79"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147881050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation of osmo-dried carrot slices and their quality evaluation during storage 渗透干燥胡萝卜片的制备及其贮藏质量评价

Indian Journal of Agricultural Biochemistry Pub Date : 2025-01-01 DOI: 10.5958/0974-4479.2025.00008.2

Neeraj Gupta, Julie D Bandral, Monika Sood, Seerat Gupta, Daman Preet Kour

{"title":"Preparation of osmo-dried carrot slices and their quality evaluation during storage","authors":"Neeraj Gupta, Julie D Bandral, Monika Sood, Seerat Gupta, Daman Preet Kour","doi":"10.5958/0974-4479.2025.00008.2","DOIUrl":"https://doi.org/10.5958/0974-4479.2025.00008.2","url":null,"abstract":"AbstractCarrots are one of the most significant veggies that are loaded with bioactive components. The aim of this study was to determine the effect of different osmotic solutions on the development of osmo-dehydrated carrot slices and their quality characteristics were analyzed along with overall acceptability scores. In the present study slices of carrot were dipped in different sugar syrup (50, 55, 60, 65, 70 and 75°Brix) followed by drying under cabinet drier. Treatment T7 (75°Brix sugar syrup) exhibited the highest mean TSS (78.71°Brix), total sugars (62.17%), reducing sugars (40.03%) and overall acceptability (8.50). Maximum ash content (2.28%) and ascorbic acid (3.59 mg/100 g) were recorded in treatment T2 (50°Brix sugar syrup), although maximum titratable acidity (1.30%) and ß-carotene (150.43 mg/100 g) were reported in treatment T1 (control). After completion of three months of storage, treatment dipped in 75°Brix sugar syrup was found to be more acceptable and nutritious than other treatments. Thus, osmo-drying of carrot slices not only enhance their shelf life but also boost their usage in ready-to-eat meals and convenient snack options.","PeriodicalId":39477,"journal":{"name":"Indian Journal of Agricultural Biochemistry","volume":"38 1","pages":"68-73"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147892026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of different solvents and extraction methods on the extraction of bioactive components and antioxidants from immature dropped kinnow fruit 不同溶剂及提取方法对未成熟金杏果实中生物活性成分及抗氧化剂提取的影响

Indian Journal of Agricultural Biochemistry Pub Date : 2023-01-01 DOI: 10.5958/0974-4479.2023.00011.4

D. Mridula, M. Bala, Simran Arora, Pawandeep Kaur Sandhu, Anusha Awasthi, S. Rathod, D. Goswami

引用次数: 0

Studies on popping quality of grains of popcorn variety- amber soaked in various concentrations of different salts and dipped in different unsaturated fats 琥珀爆米花品种籽粒在不同浓度不同盐浸泡和不同不饱和脂肪浸渍下爆轰品质的研究

Indian Journal of Agricultural Biochemistry Pub Date : 2023-01-01 DOI: 10.5958/0974-4479.2023.00007.2

Shivam Patel, Akash Patel, L. Singh, A. Katiyar, Randheer Singh

引用次数: 0

Minerals and anti-nutritional components of raw, cooked and sprouted cowpea genotypes 生豇豆、熟豇豆和发芽豇豆基因型的矿物质和抗营养成分

Indian Journal of Agricultural Biochemistry Pub Date : 2023-01-01 DOI: 10.5958/0974-4479.2023.00008.4

NP Korant, H. Ramani, AV Narwade, Ps Patel

引用次数: 0

Expression and activity analysis of genes/enzymes of nitrate assimilation at low nitrogen level in high and low nitrogen use efficient wheat genotypes at pre and post-flowering stages 高、低氮高效小麦基因型花前、花后低氮同化基因/酶的表达及活性分析

Indian Journal of Agricultural Biochemistry Pub Date : 2023-01-01 DOI: 10.5958/0974-4479.2023.00009.6

B. Abuj, U. Dalvi, V. Chimote, P. Lokhande, AA Kale, R. Naik

引用次数: 0

Study on the pretreatment methods for extraction of essential oil from lemongrass (Cymbopogon citratus), Basil (Ocimum basilicum) and coriander (Coriandrum sativum) leaves 柠檬草、罗勒、香菜叶中提取精油的预处理方法研究

Indian Journal of Agricultural Biochemistry Pub Date : 2023-01-01 DOI: 10.5958/0974-4479.2023.00016.3

S. Yadav, S. Chopra, I. Mani, VK Modi, Raj Kumar

引用次数: 0

Studies on andrographolide content and biological activities of plant extract of Andrographis paniculata from Thailand 泰国穿心莲提取物穿心莲内酯含量及生物活性研究

Indian Journal of Agricultural Biochemistry Pub Date : 2023-01-01 DOI: 10.5958/0974-4479.2023.00004.7

Worapong Kitdamrongtham, A. Manosroi, Charinya Chankhampan, C. Gupta, D. Prakash, J. Manosroi

引用次数: 0

Changes in chemical, functional and sensory properties of osmo-dried eureka lemon peel flakes during storage 渗透干燥尤里卡柠檬皮薄片在贮存过程中化学、功能和感官特性的变化

Indian Journal of Agricultural Biochemistry Pub Date : 2023-01-01 DOI: 10.5958/0974-4479.2023.00006.0

Pawandeep Kour, Neeraj Gupta, J. Singh, M. Sood, J. Bandral, Sushil Sharma

引用次数: 0

Quality studies in some promising genotypes/varieties of mung bean 绿豆一些有发展前景的基因型/品种的品质研究

Indian Journal of Agricultural Biochemistry Pub Date : 2023-01-01 DOI: 10.5958/0974-4479.2023.00015.1

A. Yadav, R. Babu, V. Gautam, Ram Ashish

引用次数: 0