Revista Espanola de Patologia最新文献

{"title":"Pursuing excellence through quality accreditation in Pathology departments","authors":"","doi":"10.1016/j.patol.2026.100893","DOIUrl":"10.1016/j.patol.2026.100893","url":null,"abstract":"","PeriodicalId":39194,"journal":{"name":"Revista Espanola de Patologia","volume":"59 3","pages":"Article 100893"},"PeriodicalIF":0.6,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148537219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rosai–Dorfman disease in the distal ileum causing intestinal obstruction","authors":"Ricardo Ceballos Arcos,&nbsp;Angela María Molina Betancourt,&nbsp;Jhoan Sebastián Cruz Barbosa,&nbsp;Andrés Felipe Valencia Cardona","doi":"10.1016/j.patol.2026.100886","DOIUrl":"10.1016/j.patol.2026.100886","url":null,"abstract":"<div><div>Rosai–Dorfman disease (RDD) is an infrequent non-Langerhans cell histiocytosis characterised by the proliferation of histiocytes with emperipolesis. Although extranodal involvement accounts for 43% of cases, gastrointestinal involvement is uncommon (&lt;1%), making isolated ileal disease extremely rare. We report the case of a 66-year-old male presenting with intestinal obstruction preceded by constitutional symptoms. Capsule endoscopy demonstrated an ulcerated, stenosing lesion in the distal ileum, resulting in capsule retention and requiring surgical intervention. Histopathological examination confirmed RDD and excluded IgG4-related disease. This case illustrates the diagnostic challenge of extranodal RDD, which must be considered as a differential diagnosis in gastrointestinal lesions with atypical behaviour, to ensure timely management and prevent complications associated with delayed diagnosis.</div></div>","PeriodicalId":39194,"journal":{"name":"Revista Espanola de Patologia","volume":"59 3","pages":"Article 100886"},"PeriodicalIF":0.6,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148302918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mesenteric cyst lined with pancreatic heterotopia: Anatomopathological findings in an exceptional case","authors":"Silvia Sánchez-Infante Carriches,&nbsp;Bairon Alfonso Paz Fernández,&nbsp;Camilo José Castellón Pavón,&nbsp;Manuel Durán Poveda","doi":"10.1016/j.patol.2026.100888","DOIUrl":"10.1016/j.patol.2026.100888","url":null,"abstract":"<div><div>Mesenteric cysts are uncommon lesions whose classification is primarily based on their histological origin. We present the case of a mesenteric cyst diagnosed incidentally during staging investigations for colonic adenocarcinoma. Histological examination revealed a multiloculated cystic lesion lined by non-mucinous columnar epithelium without atypia, associated with mature pancreatic acini. Immunohistochemical analysis showed positivity for CK7, CK19, EMA, and trypsin, and negativity for mesothelial, enteric, and lymphatic markers, thereby excluding the subtypes described in current classifications. This exceptional finding, not previously reported, broadens the histopathological spectrum of mesenteric cysts and suggests pancreatic heterotopia as a possible origin of a subtype not recognised to date.</div></div>","PeriodicalId":39194,"journal":{"name":"Revista Espanola de Patologia","volume":"59 3","pages":"Article 100888"},"PeriodicalIF":0.6,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148303010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A morphology-guided ViT–CNN approach for the detection of lung and colon cancer using H&E-stained biopsy images","authors":"Pranjal Das,&nbsp;Rajagopal Kumar,&nbsp;Dushmanta Kumar Das","doi":"10.1016/j.patol.2026.100885","DOIUrl":"10.1016/j.patol.2026.100885","url":null,"abstract":"<div><div>The accurate categorisation of histopathological images is crucial for the reliable identification of morphologically similar cancers, including lung and colon cancer. According to the WHO and IARC, lung cancer accounted for approximately 2.48 million new cases and 1.8 million deaths worldwide in 2022, making it the leading cause of cancer-related mortality worldwide. Colorectal cancer also represents a major global health burden, with more than 1.9 million new cases and approximately 930,000 deaths reported in 2020 alone. These rising burdens highlight the need for robust computational pathology systems capable of accurate morphological discrimination. In this paper, we propose MorphoViT-CNN, a hybrid model that combines a Vision Transformer for capturing global contextual information with Convolutional Neural Networks (CNNs) for extracting fine grained local features. A morphology-aware segmentation module is designed to incorporate cellular shape, size, and texture attributes, while self-distillation improves the consistency of internal representations without requiring external supervision. Supervised contrastive learning further enhances inter-class separability, particularly in label-limited settings. The model was evaluated on the LC25000 dataset using five-fold cross validation, and ablation studies were conducted to assess the contribution of each component. MorphoViT-CNN demonstrated superior accuracy and precision-recall performance compared with leading CNN and transformer-based baselines, while significantly reducing misclassification among challenging histological subtypes. Its interpretable and multi-scale architecture provides a scalable framework for integration into computational pathology workflows. Future work will explore multimodal integration and foundation model pre-training to further enhance its applicability to precision oncology.</div></div>","PeriodicalId":39194,"journal":{"name":"Revista Espanola de Patologia","volume":"59 3","pages":"Article 100885"},"PeriodicalIF":0.6,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148399554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological differentiation of hyperchromatic crowded groups (HCG) in cervical cytology. Possible clinical significance","authors":"Julio César Villarreal Ramírez,&nbsp;Jesús E. Guaithero Rivas,&nbsp;Lorena Ramírez,&nbsp;Jesús Peña Guillén,&nbsp;Morelva Toro de Méndez","doi":"10.1016/j.patol.2026.100883","DOIUrl":"10.1016/j.patol.2026.100883","url":null,"abstract":"<div><div>HCG groups (hyperchromatic crowded groups) are hyperchromatic cellular aggregates observed during routine screening for cancer and premalignant intraepithelial lesions in both negative and abnormal cervical cytology samples. This study aimed to characterise the cytomorphological features of HCG groups in cervical cytology samples, in order to classify them as benign/reactive, atypical, or potentially neoplastic, and to explore their clinical significance in this setting. Conventional cervical cytology samples that were satisfactory for analysis and exhibited HCG groups were selected. Of 2810 cytological cases, 70 (2.49%) met the inclusion criteria. HCG groups were identified in 41% of negative cytology samples and in 59% of abnormal samples. Atypical HCG groups (ASC-H) were the most common (24.28%), followed by potentially neoplastic/HSIL (17.14%). The morphology of HCG groups is variable, necessitating carefully recognition, differentiation, and interpretation, particularly in abnormal cases requiring detailed and systematic evaluation. In cases with a clear HSIL context, the presence of HCG groups composed of squamous metaplastic cells or cells with a glandular appearance may raise suspicion of intracanal HSIL, which should be confirmed by histopathological examination. Comprehensive assessment of both cellular and background features in cytological smears helps clarify the origin of HCG groups, ensuring accurate morphological characterisation and appropriate cytological classification and clinical interpretation. Clinical and epidemiological information is essential for the accurate classification of HCG groups.</div></div>","PeriodicalId":39194,"journal":{"name":"Revista Espanola de Patologia","volume":"59 3","pages":"Article 100883"},"PeriodicalIF":0.6,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148309907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Opportunistic identification of Li-Fraumeni syndrome through germline TP53 variant detection from routine tumour next-generation sequencing: An analysis of 1394 cases","authors":"Svetlana Shalygina ,&nbsp;Artemio Payá Romá ,&nbsp;Alejandro Garzón Arana ,&nbsp;Leticia Gracia Sáenz ,&nbsp;José Luís Soto Martínez ,&nbsp;Cristina Alenda González","doi":"10.1016/j.patol.2026.100868","DOIUrl":"10.1016/j.patol.2026.100868","url":null,"abstract":"<div><h3>Background and objective</h3><div>Evaluation of a hybrid strategy (bioinformatics and clinical) for detecting germline pathogenic variants in TP53 from routine tumour NGS, addressing clonal haematopoiesis of indeterminate potential (CHIP) as a key confounding factor.</div></div><div><h3>Material and methods</h3><div>Retrospective analysis of 1394 cases (2019–2025). Initial filters: VAF &gt;40%, population rarity, and ACMG pathogenicity classification. Candidate variants were further refined using the Chompret criteria, with germline confirmation via Sanger sequencing in peripheral blood.</div></div><div><h3>Results</h3><div><em>TP53</em> variants were identified in 576 (41%) cases. The bioinformatics pipeline initially flagged 222 suspected cases; clinical curation narrowed these to 14 high-suspicion cases. Of these, 12/14 (85.7%) underwent confirmatory testing, while two were lost to follow-up, introducing a verification bias that should be considered when interpreting the overall PPV. Three LFS diagnoses were confirmed (overall positive predictive value [PPV]: 21.4%; confirmation rate among tested: 25%), all in paediatric patients (two adrenocortical carcinomas and one osteosarcoma). Notably, this included a <em>de novo</em> case without a family history. Conversely, no adult cases were confirmed despite high VAFs, highlighting specificity issues in this demographic. The number needed to screen (NNS) to detect one LFS patient was 465.</div></div><div><h3>Conclusions</h3><div>Our findings underscore the clinical utility of this combined approach, particularly its high efficiency in the paediatric population where it identified a <em>de novo</em> case missed by classical criteria. However, its utility in unselected adults appears limited without orthogonal assays to exclude CHIP, suggesting that age-specific strategies are necessary for opportunistic screening.</div></div>","PeriodicalId":39194,"journal":{"name":"Revista Espanola de Patologia","volume":"59 2","pages":"Article 100868"},"PeriodicalIF":0.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147595134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Large bilateral labial fibroepithelial polyps with myxoid features: A case report and review of literature","authors":"Desiré Hernández Martínez ,&nbsp;Alejandra Mayoral Triana ,&nbsp;Alejandro Pascual Martín ,&nbsp;Luis Ortega Medina","doi":"10.1016/j.patol.2026.100862","DOIUrl":"10.1016/j.patol.2026.100862","url":null,"abstract":"<div><div>Fibroepithelial polyps are benign mesenchymal tumours that usually occur in skin folds, such as the axillae or groin. They are usually unilateral lesions measuring less than 5<!--> <!-->cm in their greatest dimension. We present the case of a 27-year-old woman with bilateral fibroepithelial polyps of the labia majora. This case is unusual not only because of the bilateral nature of the lesions, but also due to their size and the presence of a striking myxoid stroma on microscopic examination. When diagnosing vulval masses, it is essential to consider both benign and malignant conditions that may occur in this region.</div></div>","PeriodicalId":39194,"journal":{"name":"Revista Espanola de Patologia","volume":"59 2","pages":"Article 100862"},"PeriodicalIF":0.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146167312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Initial evaluation of the effectiveness of a new eco-friendly staining method: Purple corn-eosin staining","authors":"Gian Carlos Ramirez Ubillus ,&nbsp;José Sereno Aliaga ,&nbsp;Mercedes Bravo Taxa ,&nbsp;Renier Cruz Baca ,&nbsp;Gissell Tavera Veramatus ,&nbsp;Hendrick Escobar de la Cruz","doi":"10.1016/j.patol.2026.100871","DOIUrl":"10.1016/j.patol.2026.100871","url":null,"abstract":"<div><h3>Objective</h3><div>To determine the intertest agreement in the histopathological diagnosis using a new eco-friendly staining method, purple corn-eosin, compared with routine haematoxylin–eosin (H&amp;E) staining.</div></div><div><h3>Material and methods</h3><div>A cross-sectional study analysing 96 paraffin blocks from biopsies obtained at the Taxa Oncology Laboratory in Lima, Peru. New histological sections were obtained from each block, to which the new staining method was applied. These results were compared with the corresponding diagnoses from the reference H&amp;E staining.</div></div><div><h3>Results</h3><div>Using the reference method, the frequency of lesions suggestive of neoplasia or confirmed neoplasia was 39.6%. Also, at both baseline and 15 days later, observer 1 reported these diagnoses in 43.8% and 41.7% of cases, respectively, with a slightly lower frequency than observer 2 (45.8% and 42.7%, respectively). Inter-assay agreement between the purple corn-eosin method and the reference H&amp;E method was almost perfect (<em>κ</em> <!-->=<!--> <!-->0.91). Additionally, baseline interobserver agreement for the purple corn-eosin method was strong (<em>κ</em> <!-->=<!--> <!-->0.87), with a very small difference compared with the adjusted Kappa (<em>κ</em> <!-->=<!--> <!-->0.88). Similar results were observed at 15 days (<em>κ</em> <!-->=<!--> <!-->0.81 and <em>κ</em> <!-->=<!--> <!-->0.82, respectively).</div></div><div><h3>Conclusion</h3><div>This proposed staining method demonstrated optimal statistical reliability, in addition to eliminating the toxicity associated with its preparation compared with routine staining, suggesting a great potential for application in pathology laboratories worldwide.</div></div>","PeriodicalId":39194,"journal":{"name":"Revista Espanola de Patologia","volume":"59 2","pages":"Article 100871"},"PeriodicalIF":0.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147594966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extracranial schwannomas of peripheral nervous system: An aesthetic challenge – A comprehensive analysis of 38 cases","authors":"Samikshya Thapa ,&nbsp;Ravi Hari Phulware ,&nbsp;Gajendra Kumar Yadav ,&nbsp;Ashok Singh ,&nbsp;Prashant Durgapal ,&nbsp;Rahul Kumar ,&nbsp;Amit Gupta ,&nbsp;Sanjeev Kishoree ,&nbsp;Arvind Kumar","doi":"10.1016/j.patol.2026.100858","DOIUrl":"10.1016/j.patol.2026.100858","url":null,"abstract":"<div><h3>Background</h3><div>Schwannomas are benign, slow-growing tumours that arise from Schwann cells. Extracranial schwannomas are less common than those occurring at the intracranial vestibulocochlear and cerebellopontine angles. Because extracranial schwannomas are uncommon, it is difficult to make an accurate clinical diagnosis. Therefore, histopathological examination is the gold standard.</div></div><div><h3>Methods</h3><div>In this study, we aim to describe the incidence, presenting clinical features, anatomical location, size, and initial (presumption) diagnosis of thirty-eight cases that were histopathologically diagnosed as extracranial schwannomas, along with a literature review. A retrospective, cross-sectional observational study was conducted at the Department of Pathology and Laboratory Medicine, All India Institute of Medical Science, Rishikesh, India, from January 2018 to June 2024. Data and histopathological slides were retrieved and analysed.</div></div><div><h3>Results</h3><div>Thirty-eight patients were documented, with an age range of 11–75 years and a mean age of 37 years, and a male predominance (M:F, 2.8:1) was noted. Tumour size ranged from 0.3 to 13<!--> <!-->cm. Thirteen cases of schwannomas occurred in the head and neck (34.21%), eleven in the spine (29%), six in the upper extremities (15.79%), three each in the lower extremities (7.88%) and the upper trunk (7.88%) and two in the lower trunk/visceral region (5.24%). Most patients presented with painless swellings. The study indicates that schwannomas were most commonly found in the head and neck region, followed by the spine. Within the extremities, they were more frequent in the upper extremities, while in the trunk, they were found in the chest wall, ribs, posterior mediastinum (part of the chest cavity), adrenal gland, and pararenal area.</div></div><div><h3>Conclusions</h3><div>The study indicates that schwannomas are most commonly found in the head and neck region, followed by the spine. Within the extremities, they occur more frequently in the upper extremities, while in the trunk they were found in the chest wall, ribs, posterior mediastinum (part of the chest cavity), adrenal gland, and pararenal area. The radioresistant nature of schwannomas alerts both clinicians and pathologists to the importance of early diagnosis and to be familiar with their uncommon anatomical locations.</div></div>","PeriodicalId":39194,"journal":{"name":"Revista Espanola de Patologia","volume":"59 2","pages":"Article 100858"},"PeriodicalIF":0.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146081711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Post-mortem ultrasound-guided biopsies: A valuable tool for investigating novel diseases","authors":"Ánxela Vidal González ,&nbsp;Juan José Páez Vargas ,&nbsp;Ana María Vicente Montaña ,&nbsp;Mariona Cabero Piris ,&nbsp;José Ramón Fortes Alen ,&nbsp;Miguel de Górgolas Hernandéz-Mora ,&nbsp;Laura Prieto Pérez","doi":"10.1016/j.patol.2026.100863","DOIUrl":"10.1016/j.patol.2026.100863","url":null,"abstract":"<div><h3>Introduction</h3><div>While autopsies are the gold standard for investigating infectious diseases, their use is limited by infrastructure, staffing, or safety concerns during outbreaks. Post-mortem ultrasound-guided biopsies offer a practical, minimally invasive alternative for tissue sampling.</div></div><div><h3>Methods</h3><div>This case series included 60 deceased COVID-19 patients from Intensive Care Units (ICUs) and hospital wards (HWs). A total of 108 post-mortem specimens were obtained, including five necropsies, three of which were complemented by post-mortem biopsies. Percutaneous biopsies were performed at bedside within the first hour post-mortem, while necropsies were performed between 3<!--> <!-->h and 3 days after death. Samples were analysed using morphological, immunohistochemical (IHC), and ultrastructural techniques, including Transmission Electron Microscopy (TEM). Epidemiological and clinical data were collected prior to death.</div></div><div><h3>Results</h3><div>Necropsies provided lung, heart, kidney, liver, spleen, bone marrow, and testicular samples, with procedure durations ranging from 3 to 5<!--> <!-->h. Tru-cut biopsies were faster, less invasive, and more cost-effective, with an average cost of €180 compared to €1500–€3000 for autopsies. High-quality samples for IHC and TEM analysis were obtained, with a success rate of 83% for lung biopsies, 100% for liver, 79% for kidney, and 67% for spleen samples. The minimally invasive nature of biopsies improved acceptance by families, particularly in cases when full autopsies were not feasible.</div></div><div><h3>Conclusions</h3><div>Post-mortem biopsies represent a valuable alternative to autopsies, providing high-quality samples, improved accessibility, lower costs, and faster processing. Their minimally invasive nature and ability to obtain key organ samples enhance feasibility in both clinical and research settings, supporting broader application in the investigation of emerging diseases.</div></div>","PeriodicalId":39194,"journal":{"name":"Revista Espanola de Patologia","volume":"59 2","pages":"Article 100863"},"PeriodicalIF":0.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146167335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}