Revista Espanola de Patologia最新文献

Recomendaciones de la SEAP-IAP para la recolección, el almacenamiento y el uso de materiales biológicos de origen humano y los datos relacionados, destinados a la investigación. Consideración genérica de biobanco y revisión ético-legal (Parte II) SEAP-IAP 关于收集、储存和使用源自人类的生物材料及相关研究数据的建议。一般性生物库考虑和伦理-法律审查（第 II 部分）

Revista Espanola de Patologia Pub Date : 2024-10-01 DOI: 10.1016/j.patol.2024.04.005

Antonio Martínez Lorente , Esther Rosello Sastre , María Jesús Fernández Aceñero , Lara Zaragoza Macián , Javier Azúa Romeo , Clara Alfaro-Cervelló , Samuel Navarro , Eugenia García Fernández , Jordi Temprana-Salvador , Mar Iglesias Coma , Francesc Olivares Vegas , Maite Fernández Figueras , Sonsoles Aso Manso , José Javier Aguirre Anda , José Santos Salas Valién , Ramiro Álvarez Alegret , Javier Hernández Losa , Cristina Jou Muñoz , Carme Dinarès Fernández , Marina Urbano Carrillo , Francesc Tresserra Casas

{"title":"Recomendaciones de la SEAP-IAP para la recolección, el almacenamiento y el uso de materiales biológicos de origen humano y los datos relacionados, destinados a la investigación. Consideración genérica de biobanco y revisión ético-legal (Parte II)","authors":"Antonio Martínez Lorente , Esther Rosello Sastre , María Jesús Fernández Aceñero , Lara Zaragoza Macián , Javier Azúa Romeo , Clara Alfaro-Cervelló , Samuel Navarro , Eugenia García Fernández , Jordi Temprana-Salvador , Mar Iglesias Coma , Francesc Olivares Vegas , Maite Fernández Figueras , Sonsoles Aso Manso , José Javier Aguirre Anda , José Santos Salas Valién , Ramiro Álvarez Alegret , Javier Hernández Losa , Cristina Jou Muñoz , Carme Dinarès Fernández , Marina Urbano Carrillo , Francesc Tresserra Casas","doi":"10.1016/j.patol.2024.04.005","DOIUrl":"10.1016/j.patol.2024.04.005","url":null,"abstract":"<div><div>The working group set up by the SEAP-IAP addresses in this <em>Part II</em> some general considerations and five particular considerations to be taken into account when a biological sample of human origin, coming from our archives, acquires a different destination from the usual one, in this case for research. From this moment on, we must follow mandatory legal and ethical rules, and the different recitals provide us with guidelines to ensure good practice, both for biological material and its associated data. The traditional task of custody given to the Pathological Anatomy is approached, as always, from the point of view of responsibility and, in this article, adjusted to its time.</div></div>","PeriodicalId":39194,"journal":{"name":"Revista Espanola de Patologia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141843770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of B-cell lymphoma 2 (BCL-2) expression in disordered proliferative endometrium and simple endometrial hyperplasia B 细胞淋巴瘤 2 (BCL-2) 在增生紊乱性子宫内膜和单纯性子宫内膜增生症中的表达比较

Revista Espanola de Patologia Pub Date : 2024-10-01 DOI: 10.1016/j.patol.2024.05.005

Zahra Ghorbanniadelavar , Mohammadreza Jalali Nadoushan , Masood Soltanipur

{"title":"Comparison of B-cell lymphoma 2 (BCL-2) expression in disordered proliferative endometrium and simple endometrial hyperplasia","authors":"Zahra Ghorbanniadelavar , Mohammadreza Jalali Nadoushan , Masood Soltanipur","doi":"10.1016/j.patol.2024.05.005","DOIUrl":"10.1016/j.patol.2024.05.005","url":null,"abstract":"<div><h3>Background and objective</h3><div>B-cell lymphoma-2 (BCL-2) is an anti-apoptotic protein that may play a role in disordered proliferative endometrium (DPE) and endometrial hyperplasia (EH). Several studies have investigated BCL-2 expression in normal, hyperplastic endometrium and endometrial adenocarcinoma, with conflicting results. Therefore, the present study aimed to compare the expression of BCL-2 in disordered proliferative endometrium and simple EH.</div></div><div><h3>Methods</h3><div>In this cross-sectional study, 63 DPE and 67 SEH samples from patients referred to Mostafa Khomeini Hospital between 2017 and 2022 were immunohistochemically stained by BCL-2 antibody. BCL-2 expression in each sample was reported as negative, weak positive, and strong positive. The findings were analyzed using SPSS version 16 software.</div></div><div><h3>Results</h3><div>Negative, weakly positive, and strongly positive BCL-2 expression was observed in 55.6%, 38.1%, and 6.3% of DPE samples, and 61.2%, 31.3%, and 7.5% of SEH samples, respectively, which does not show a statistically significant difference (<em>p</em>  =0.378 and <em>p</em>  <!-->0.178, respectively).</div></div><div><h3>Conclusion</h3><div>BCL-2 expression is observed with a relatively similar frequency in DPE and SEH samples, and it is probably under the control of oestrogen hormone as the main factor involved in the pathogenesis of these lesions.</div></div>","PeriodicalId":39194,"journal":{"name":"Revista Espanola de Patologia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141713264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

¿QUO VADIS CAR-T? [车在哪里？ ]

Revista Espanola de Patologia Pub Date : 2024-10-01 DOI: 10.1016/j.patol.2024.09.001

Dr. Francisco Javier Menárguez Palanca

引用次数: 0

Tiempos y condiciones de almacenamiento de las muestras en anatomía patológica. Recomendaciones de la Sociedad Española de Anatomía Patológica parte 1: muestras destinadas al diagnóstico [解剖病理学样本的储存时间和条件。西班牙解剖病理学会（SEAP）建议第 1 部分：诊断样本]。

Revista Espanola de Patologia Pub Date : 2024-10-01 DOI: 10.1016/j.patol.2024.04.004

Francesc Tresserra Casas , Esther Rosello Sastre , María Jesús Fernández Aceñero , Lara Zaragoza Macián , Javier Azúa Romeo , Clara Alfaro-Cervelló , Samuel Navarro Fos , Eugenia García Fernández , Jordi Temprana-Salvador , Mar Iglesias Coma , Francesc Olivares Vegas , Maite Fernández Figueras , Sonsoles Aso Manso , José Javier Aguirre Anda , José Santos Salas Valién , Ramiro Álvarez Alegret , Javier Hernández Losa , Cristina Jou Muñoz , Carme Dinarès Fernández , Marina Urbano Carrillo , Antonio Martínez Lorente

{"title":"Tiempos y condiciones de almacenamiento de las muestras en anatomía patológica. Recomendaciones de la Sociedad Española de Anatomía Patológica parte 1: muestras destinadas al diagnóstico","authors":"Francesc Tresserra Casas , Esther Rosello Sastre , María Jesús Fernández Aceñero , Lara Zaragoza Macián , Javier Azúa Romeo , Clara Alfaro-Cervelló , Samuel Navarro Fos , Eugenia García Fernández , Jordi Temprana-Salvador , Mar Iglesias Coma , Francesc Olivares Vegas , Maite Fernández Figueras , Sonsoles Aso Manso , José Javier Aguirre Anda , José Santos Salas Valién , Ramiro Álvarez Alegret , Javier Hernández Losa , Cristina Jou Muñoz , Carme Dinarès Fernández , Marina Urbano Carrillo , Antonio Martínez Lorente","doi":"10.1016/j.patol.2024.04.004","DOIUrl":"10.1016/j.patol.2024.04.004","url":null,"abstract":"<div><h3>Introduction</h3><div>The correct storage of specimens in the Pathology service is of vital importance for patient safety. However, there are no clear recommendations as regarding how long samples should be stored for a minimum period.</div></div><div><h3>Material and methods</h3><div>A working group of the Spanish Society of Anatomic Pathology has reviewed a series of recommendations established in the literature and after two rounds of consultations and a discussion and voting phase has established a series of storage time proposals.</div></div><div><h3>Results</h3><div>Each of the proposals is presented with the data found in the literature and sometimes offers definitions and exceptions to the proposal.</div></div><div><h3>Conclusion</h3><div>These recommendations, which are minimums, establish a period of at least 10 years for paraffin embedded blocks (including cell blocks), histological preparations, general cytology, pathologic cervico-vaginal cytology and electron microscopy blocks; at least 3 years for cervico-vaginal cytology, 5 years for extracted nucleic acids, at least 4 weeks for tissue in formalin and from the time of diagnosis for liquid cytology material and fluids.</div></div>","PeriodicalId":39194,"journal":{"name":"Revista Espanola de Patologia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142407006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cuantificación del CDX2 mediante el H-Score y su valor pronóstico en el cáncer colorrectal [使用 H-Score 定量 CDX2 及其在结肠癌中的预后价值]。

Revista Espanola de Patologia Pub Date : 2024-10-01 DOI: 10.1016/j.patol.2024.06.007

Adriano Martínez-Aracil

{"title":"Cuantificación del CDX2 mediante el H-Score y su valor pronóstico en el cáncer colorrectal","authors":"Adriano Martínez-Aracil","doi":"10.1016/j.patol.2024.06.007","DOIUrl":"10.1016/j.patol.2024.06.007","url":null,"abstract":"<div><div>Colorectal cancer is the third tumor with the highest incidence in the world population and is the second cause of death according to the Globocan study. CDX2 has been acquiring an important role as a sensitive and specific marker in the diagnosis of colorectal cancer. However, the lack of inclusion of this marker in the pathology guidelines together with the lack of existing studies prevent its daily use. Although multiple studies relate the absence of staining to a worse prognosis, the literature does not define how intense the staining must be to be considered positive or negative. In the present study, the H-Score is described as a method to determine the positivity of CDX2 staining, using free access software called QuPath with a sample of 169 patients. Furthermore, it is suggested that those patients whose tumors had an H-Score for CDX2 less than or equal to 152 points had a significantly shorter recurrence-free interval time compared to those with an H-Score greater than this threshold. For this reason, this study aims to highlight the importance of quantification using digital pathology, as it could be applied in daily practice, and suggests a reference value for CDX2 from which the tumor prognosis may differ.</div></div>","PeriodicalId":39194,"journal":{"name":"Revista Espanola de Patologia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142407004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diagnóstico de metástasis ganglionares de carcinoma papilar tiroideo midiendo tiroglobulina en la aguja de la punción. Cálculo de punto de corte optimo en nuestra serie 通过测量穿刺针中的甲状腺球蛋白诊断甲状腺乳头状癌淋巴结转移。在我们的系列研究中计算出最佳临界点。

Revista Espanola de Patologia Pub Date : 2024-10-01 DOI: 10.1016/j.patol.2024.05.003

Francisco García-Molina , Julian Jesus Arense-Gonzalo , Alfonso Aguera-Sanchez , Emilio Peña-Ros , Miguel Ruiz-Marín , Matias Matínez-Perez , Asunción Chaves-Benito , Francisco Martínez-Diaz

{"title":"Diagnóstico de metástasis ganglionares de carcinoma papilar tiroideo midiendo tiroglobulina en la aguja de la punción. Cálculo de punto de corte optimo en nuestra serie","authors":"Francisco García-Molina , Julian Jesus Arense-Gonzalo , Alfonso Aguera-Sanchez , Emilio Peña-Ros , Miguel Ruiz-Marín , Matias Matínez-Perez , Asunción Chaves-Benito , Francisco Martínez-Diaz","doi":"10.1016/j.patol.2024.05.003","DOIUrl":"10.1016/j.patol.2024.05.003","url":null,"abstract":"<div><div>Fine-needle aspiration cytology (FNAC), used in suspicious cervical lymph nodes of unknown origin is frequently inconclusive and prone to false negatives. In order to evaluate the usefulness of measuring thyroglobulin in the washing with saline solution of the puncture needle for the diagnosis of metastasis of papillary carcinoma of the thyroid, an optimal thyroglobulin cutting point has to be calculated, being positive or negative depending on whether the thyroglobulin levels are higher or lower than the cutting point.</div><div>We have retrospectively studied 33 patients (19 women and 14 men) with an average age of 49.3 years, with papillary carcinoma of the thyroid and suspected lymph node metastasis. Of them 16 (47.1%) had a positive FNAC. To determine thyroglobulin predictive capacity with regards to the metastasis of papillary carcinoma of the thyroid a ROC analysis was carried out with an under curve area UCA: 0.987 (CI 95%: 0.808-1.000) obtaining, using Youden's J statistic, 0.4 ng/ml as the thyroglobulin cutting point with best predictive capacity. The study of the relationship between thyroglobulin and the preservation/non-preservation of the thyroid showed statistically significant differences (<em>P</em>=.023).</div><div>Our results validate 0.4 ng/ml of thyroglobulin as an optimal cutting point of the presence of metastasis of papillary carcinoma of the thyroid in lymph nodes. When reviewing the bibliography, a great diversity of cutting points may be found, which is explained mainly by the great inter-observer and inter-assay variability. That is why we recommend calculating each laboratory's own optimal cutting point; and determine in subsequent studies two cutting points depending on whether or not thyroid is preserved.</div></div>","PeriodicalId":39194,"journal":{"name":"Revista Espanola de Patologia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141851086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunohistochemical evaluation of cyclin D1 and p63 in odontogenic keratocyst and unicystic ameloblastoma 牙源性角化囊肿和单囊性母细胞瘤中细胞周期蛋白 D1 和 p63 的免疫组化评估

Revista Espanola de Patologia Pub Date : 2024-10-01 DOI: 10.1016/j.patol.2024.06.006

Enrico Escobar , Fernán Gómez-Valenzuela , Cristian Peñafiel , Eduardo Chimenos-Küstner , Ricardo Pérez-Tomás

{"title":"Immunohistochemical evaluation of cyclin D1 and p63 in odontogenic keratocyst and unicystic ameloblastoma","authors":"Enrico Escobar , Fernán Gómez-Valenzuela , Cristian Peñafiel , Eduardo Chimenos-Küstner , Ricardo Pérez-Tomás","doi":"10.1016/j.patol.2024.06.006","DOIUrl":"10.1016/j.patol.2024.06.006","url":null,"abstract":"<div><h3>Introduction</h3><div>Odontogenic keratocyst (OKC) and unicystic ameloblastoma (UA) are lesions of odontogenic origin. Both lesions are morphologically cysts. However, they are classified as developmental cysts and epithelial odontogenic tumours, respectively. Cyclin D1 (CCD1) dysregulation is associated with oncogenic activity and malignancies, while tumour protein p63 (p63) alterations are associated with tumourigenesis.</div></div><div><h3>Aim</h3><div>To evaluate and compare the protein expression of CCD1 and p63 in sporadic OKC (OKC-sp), syndromic OKC (OKC-sy), and UA.</div></div><div><h3>Material and methods</h3><div>45 cases from the Anatomical Pathology Department, Faculty of Dentistry, University of Chile were analysed and divided into groups: OKC-sp (<em>n</em>  =15) and UA (<em>n</em>  =7) and mural (<em>n</em>  <span>8). Immunohistochemical staining for CCD1 and p63 proteins was performed from paraffin-embedded sections. Statistical analysis included the Shapiro–Wilk test, one-way ANOVA with Tukey's multiple comparisons, and Spearman's correlation coefficient (</span><em>p</em>  <0.001). No correlation was found between CCD1 and p63 expression.</div></div><div><h3>Conclusion</h3><div>P63 may serve as a valuable marker for evaluating cell proliferative activity in odontogenic cystic lesions, providing insights into the aggressive behaviour of mural UA.</div></div>","PeriodicalId":39194,"journal":{"name":"Revista Espanola de Patologia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141846473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mujer de 49 años con clínica urinaria de larga evolución. Estudio histológico e inmunohistoquímico de un caso de mullerianosis vesical 49岁女性，长期泌尿系统症状。一例膀胱穆勒氏病的组织学和免疫组化研究。

Revista Espanola de Patologia Pub Date : 2024-10-01 DOI: 10.1016/j.patol.2024.05.002

Laura Sánchez Godoy, María Isabel Oviedo Ramírez

引用次数: 0

Recurrencia de glomerulonefritis postrasplante renal: características clínico-patológicas 肾移植后肾小球肾炎复发：临床病理特征

Revista Espanola de Patologia Pub Date : 2024-10-01 DOI: 10.1016/j.patol.2024.06.004

Janine Andrea Orejuela , Andrés Felipe Lozano , Alejandra Taborda-Murillo , Luis Fernando Arias , Sigifredo Ospina O

{"title":"Recurrencia de glomerulonefritis postrasplante renal: características clínico-patológicas","authors":"Janine Andrea Orejuela , Andrés Felipe Lozano , Alejandra Taborda-Murillo , Luis Fernando Arias , Sigifredo Ospina O","doi":"10.1016/j.patol.2024.06.004","DOIUrl":"10.1016/j.patol.2024.06.004","url":null,"abstract":"<div><h3>Introduction</h3><div>Glomerulonephritis (GN) is one of the main causes of end-stage renal disease worldwide and therefore a frequent cause of kidney transplantation, with the possibility of recurrence of GN (Recurrent Glomerulonephritis [GNR]) in the transplanted kidney. The purpose of this study was to identify the clinic and pathological characteristics of GNR in a population of transplant patients.</div></div><div><h3>Materials and methods</h3><div>A descriptive, retrospective study was carried out in 109 patients in whom GNR was documented in the transplanted kidney demonstrated by biopsy during the period between 1998-2021.</div></div><div><h3>Results</h3><div>Of 109 patients, the most frequent GNR was GNIgA, in 38.5% (42), followed by FSGS with 31.2% (34); These same entities were the ones that presented the greatest graft dysfunction, with 50% (21) and 26.2% (11) respectively. The ranges of proteinuria indicated by the biopsy were 31.2% (34) with a range of 500 to 3500mg/24h and 34.9% (38) with proteinuria >3500mg/24h. In relation to the time elapsed between the transplant and the diagnosis of GNR, 33% (36) of the cases were >5 years, followed by 1 to 5 years in 26.6% (29). Recurrence in patients with GNIgA occurred mostly after 5 years post-transplant with 45.2% (19) and for FSGS it was between 1 and 6 months.</div></div><div><h3>Conclusion</h3><div>We found a general frequency of GNR presentation similar to those reported by other centers where biopsies are performed for clinical indication, finding that the GN that recurred most frequently are GNIgA and FSGS.</div></div>","PeriodicalId":39194,"journal":{"name":"Revista Espanola de Patologia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141840364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alveolar capillary dysplasia with misalignment of the pulmonary veins: A surgical lung biopsy and autopsy in a full-term newborn 肺泡毛细血管发育不良伴肺静脉错位：一名足月新生儿的手术肺活检和尸检结果

Revista Espanola de Patologia Pub Date : 2024-10-01 DOI: 10.1016/j.patol.2024.06.005

Carmen Rodríguez García, Cecilia López Valdivia, Jaime Ferrer Lozano, Nuria Mancheño Franch

引用次数: 0