Revista Espanola de Patologia最新文献

Systematic review and meta-analysis of the Papanicolaou Society of Cytopathology (PSC) system in diagnosing pancreatic lesions: Evaluating diagnostic accuracy

Revista Espanola de Patologia Pub Date : 2025-04-09 DOI: 10.1016/j.patol.2025.100820

Sana Ahuja , Marzieh Fattahi-Darghlou , Sufian Zaheer

{"title":"Systematic review and meta-analysis of the Papanicolaou Society of Cytopathology (PSC) system in diagnosing pancreatic lesions: Evaluating diagnostic accuracy","authors":"Sana Ahuja , Marzieh Fattahi-Darghlou , Sufian Zaheer","doi":"10.1016/j.patol.2025.100820","DOIUrl":"10.1016/j.patol.2025.100820","url":null,"abstract":"<div><h3>Introduction</h3><div>The Papanicolaou system plays a vital role in cytological evaluation, particularly in the diagnosis of pancreatic lesions. However, there is a lack of comprehensive evaluation regarding its diagnostic accuracy in pancreatic fine needle aspirations (FNAs). This study seeks to address this gap by conducting a systematic review and meta-analysis to assess the diagnostic accuracy of the Papanicolaou Society of Cytopathology (PSC) system in diagnosing pancreatic lesions.</div></div><div><h3>Methods</h3><div>A systematic search of PubMed and EMBASE databases was conducted up to March 20, 2024, using predefined search terms related to pancreas, fine needle aspiration biopsy (FNAB), and diagnostic accuracy. The selected articles were assessed for risk of bias using the QUADAS-2 tool. A meta-analysis for sensitivity and specificity for each cut-off – namely, “Atypical considered positive”, “Neoplastic considered positive”, “Suspicious of Malignancy considered positive,” and “Malignant considered positive” – was performed after excluding inadequate samples in each study. To assess diagnostic accuracy, summary receiver operating characteristic (sROC) curves were constructed, and the diagnostic odds ratio (DOR) was pooled.</div></div><div><h3>Results</h3><div>Twelve studies met the inclusion criteria, comprising a total of 3604 cases. Sensitivity and specificity varied across the PSC categories, with the “Malignant” category showing the highest sensitivity (70%) and specificity (99%). The “Suspicious for malignancy and higher risk categories” demonstrated high sensitivity (81%) and specificity (97%).</div></div><div><h3>Conclusion</h3><div>This meta-analysis underscores the accuracy of the PSC system in reporting pancreatic aspirates. It highlights the significance of the “Suspicious” and “Malignant” categories in diagnosing malignancy, as well as the “Benign” and “Atypical” categories in ruling out malignancy.</div></div>","PeriodicalId":39194,"journal":{"name":"Revista Espanola de Patologia","volume":"58 3","pages":"Article 100820"},"PeriodicalIF":0.0,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143799010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pulmonary angiostrongyliasis: Two cases of atypical manifestations of Angiostrongylus costaricensis in Guatemala

Revista Espanola de Patologia Pub Date : 2025-04-08 DOI: 10.1016/j.patol.2025.100821

Juan Carlos Santis-Mejía, Roberto Orozco, Victor Argueta

引用次数: 0

Cytomorphological patterns in bronchoalveolar lavage in the diagnostic evaluation of pulmonary diseases

Revista Espanola de Patologia Pub Date : 2025-03-17 DOI: 10.1016/j.patol.2025.100803

Smriti Sharan, Niti Sureka, Charanjeet Ahluwalia, Sana Ahuja, Sunil Ranga

{"title":"Cytomorphological patterns in bronchoalveolar lavage in the diagnostic evaluation of pulmonary diseases","authors":"Smriti Sharan, Niti Sureka, Charanjeet Ahluwalia, Sana Ahuja, Sunil Ranga","doi":"10.1016/j.patol.2025.100803","DOIUrl":"10.1016/j.patol.2025.100803","url":null,"abstract":"<div><h3>Background</h3><div>Bronchoalveolar lavage (BAL) cytology is a well-established diagnostic tool for respiratory tract lesions. The analysis of cellular patterns in BAL cytology provides useful information about infectious, inflammatory, and neoplastic lung diseases. This study aimed to analyze bronchoalveolar lavage fluid to investigate various cellular patterns in BAL cytology in different pulmonary diseases.</div></div><div><h3>Material and methods</h3><div>This retrospective study was conducted in the Department of Pathology and included BAL cytology samples from 182 patients between January 2022 and July 2023.</div></div><div><h3>Results</h3><div>Of the 182 cases, 116 (63.7%) were male and 66 (36.3%) were female. Thirty-seven (20%) cases showed a predominantly neutrophilic cellular pattern, while 61 (33.5%) showed a lymphocytic pattern. Two (1%) cases showed a mixed cellular pattern, including eosinophils. In 32 (17.5%) cases, patterns indicating specific aetiology were identified, the most common being AFB-positive granulomatous inflammation (4.3%), followed by fungal hyphae (1.6%). Malignant cells were seen in 15 (8.2%) cases, with the most common pattern being cells arranged in an acinar pattern, indicative of adenocarcinoma. A rare case of pulmonary alveolar proteinosis was diagnosed based on the presence of PAS-positive extracellular granular to globular material in cytology.</div></div><div><h3>Conclusion</h3><div>In the era of minimally invasive procedures, BAL cytology analysis stands out as a valuable tool for diagnosing various lung diseases. It aids in the diagnostic workup for many diseases, including those of tubercular, fungal, and neoplastic aetiology. Combining clinical details, radiological information, and BAL cytology can help provide a definitive diagnosis and avoid invasive procedures.</div></div>","PeriodicalId":39194,"journal":{"name":"Revista Espanola de Patologia","volume":"58 2","pages":"Article 100803"},"PeriodicalIF":0.0,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143631826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative analysis of Ki-67, α-SMA, and MMP-9 expression in oral submucous fibrosis and oral leukoplakia with/without dysplasia: Insights into malignant transformation mechanisms

Revista Espanola de Patologia Pub Date : 2025-03-17 DOI: 10.1016/j.patol.2025.100804

Sneha Masne Deshpande , Sheetal Choudhary , Sangeeta Patankar , Palak Khetan , Girish Sadaphule , Sanpreet Singh Sachdev

{"title":"Comparative analysis of Ki-67, α-SMA, and MMP-9 expression in oral submucous fibrosis and oral leukoplakia with/without dysplasia: Insights into malignant transformation mechanisms","authors":"Sneha Masne Deshpande , Sheetal Choudhary , Sangeeta Patankar , Palak Khetan , Girish Sadaphule , Sanpreet Singh Sachdev","doi":"10.1016/j.patol.2025.100804","DOIUrl":"10.1016/j.patol.2025.100804","url":null,"abstract":"<div><h3>Background</h3><div>The present study aimed to comparatively analyze the immunoexpression of Ki-67, Alpha Smooth Muscle Actin (α-SMA), and Matrix Metalloproteinase-9 (MMP-9) between Oral Leukoplakia (OL) and Oral Submucous Fibrosis (OSMF), both with and without dysplasia. The objective was to determine the potential role of these biomarkers in the diagnosis and prognostication of oral potentially malignant disorders (OPMDs) and their potential as targets for interventions.</div></div><div><h3>Materials and methods</h3><div>Immunohistochemical staining for Ki-67, α-SMA, and MMP-9 was performed on seventy formalin-fixed paraffin-embedded tissue blocks, comprising normal buccal mucosa (<em>n</em>  =30), and OL (<em>n</em>  <0.001) in OSMF compared to OL for the respective dysplastic counterparts. Additionally, OSMF with dysplasia exhibited a significantly higher expression (<em>p</em>  <!-->0.001) of these markers compared to OSMF without dysplasia.</div></div><div><h3>Conclusions</h3><div>The present study reports differences in cell proliferation rates, myofibroblast and MMP-9 expression between OSMF and OL. The higher expression of these markers in OSMF with dysplasia suggests accelerated disease progression and enhanced potential for transformation into oral squamous cell carcinoma (OSCC). These findings highlight variations in the tissue microenvironment of OPMDs, influencing their biological behaviour and prognosis.</div></div>","PeriodicalId":39194,"journal":{"name":"Revista Espanola de Patologia","volume":"58 2","pages":"Article 100804"},"PeriodicalIF":0.0,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143631827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of HPV detection and p16-Ki67 expression on prognosis in anal cancer patients

Revista Espanola de Patologia Pub Date : 2025-03-13 DOI: 10.1016/j.patol.2025.100806

Susana Pineda Contreras , Aracelly Quiroz Lagos , Joel Herrera Soto , Cristian Reyes Vergara , Tiare de la Barra Vivallos , Eliu Elgorriaga Islas , Sonia Montenegro Heredia

{"title":"Impact of HPV detection and p16-Ki67 expression on prognosis in anal cancer patients","authors":"Susana Pineda Contreras , Aracelly Quiroz Lagos , Joel Herrera Soto , Cristian Reyes Vergara , Tiare de la Barra Vivallos , Eliu Elgorriaga Islas , Sonia Montenegro Heredia","doi":"10.1016/j.patol.2025.100806","DOIUrl":"10.1016/j.patol.2025.100806","url":null,"abstract":"<div><h3>Introduction</h3><div>HPV infection has been associated with squamous cell carcinoma of the head and neck, genital tract and anal canal. HPV has two oncogenic genes, including E6 and E7, which are responsible for carcinogenesis. Ki67 and p16 have been used as biomarkers of HPV genome integration in the host cell.</div></div><div><h3>Aim</h3><div>To analyse the prognostic role of HPV status and p16/Ki67 expression in malignant lesions of the anal canal.</div></div><div><h3>Methods</h3><div>A retrospective study conducted from 2013 to 2016, including 40 biopsies.</div></div><div><h3>Results</h3><div>Histologic classification of the samples was: 9 samples of invasive carcinoma (ASCC); 9 of anal intraepithelial neoplasia (AIN) II/III; 8 condylomas; 14 non-tumoral lesions. For HPV detection we used nested-real time PCR for E6/E7. The determination of p16INK4a and Ki67 was carried out by immunohistochemistry. Additionally, demographic information was analysed. Among the 9 ASCC cases, 8 were p16-Ki67 positive and high-risk HPV positive. Of the 9 AIN II/III cases, 8 (88.8%) were HR-HPV and p16-Ki67 positive; all cancer cases were HPV-16. Out of the 8 condyloma cases, 2 (25%) were HR/LR HPV, 5 (62.5%) were LR-HPV, and 100% p16/Ki67 negative. Of the 14 non-tumoral lesions, all biomarkers tested negative.</div></div><div><h3>Discussion</h3><div>High- and low-risk HPV genotyping helps predict the prognosis of anal canal lesions. High-risk HPV infection and p16 overexpression are associated with malignant tumoral lesions.</div></div>","PeriodicalId":39194,"journal":{"name":"Revista Espanola de Patologia","volume":"58 2","pages":"Article 100806"},"PeriodicalIF":0.0,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143620628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Nodular hidradenoma: Case report and brief literature review

Revista Espanola de Patologia Pub Date : 2025-02-28 DOI: 10.1016/j.patol.2025.100801

Miguel Mansilla-Polo , Josselyn Andrea Hernández-Chinchilla , Margarita Llavador-Ros , Rafael Botella-Estrada

引用次数: 0

Epidemiology of glomerular diseases in a Colombian population

Revista Espanola de Patologia Pub Date : 2025-02-25 DOI: 10.1016/j.patol.2025.100813

Joaquín Rodelo-Ceballos , Wilson Fernando Chávez-Gómez , Alejandra Taborda-Murillo , Luis Fernando Arias

{"title":"Epidemiology of glomerular diseases in a Colombian population","authors":"Joaquín Rodelo-Ceballos , Wilson Fernando Chávez-Gómez , Alejandra Taborda-Murillo , Luis Fernando Arias","doi":"10.1016/j.patol.2025.100813","DOIUrl":"10.1016/j.patol.2025.100813","url":null,"abstract":"<div><h3>Background</h3><div>Glomerular diseases are a diverse group of conditions with variations in clinical presentation, pathophysiology, diagnosis, and treatment. Renal biopsy is essential for determining their aetiology and prognosis.</div></div><div><h3>Objective</h3><div>To describe the epidemiology of glomerular diseases based on histopathological studies conducted in the Department of Pathology at the School of Medicine, University of Antioquia, Colombia, between 1988 and 2023.</div></div><div><h3>Materials and methods</h3><div>A retrospective, descriptive, cross-sectional study was conducted on patients with glomerular disease who underwent renal biopsy between 1988 and 2023. A descriptive analysis was performed on their sociodemographic, clinical, laboratory, and pharmacological characteristics.</div></div><div><h3>Results</h3><div>A total of 4567 histopathology reports were analysed. Of these, 56.6% were from women, with an average age of 32 years. Nephrotic syndrome was the most common indication for renal biopsy (35.0%). Lupus nephritis was the most prevalent diagnosis (28.2%), followed by focal segmental glomerulosclerosis (16.6%). In adults, lupus nephritis and focal segmental glomerulosclerosis were the most frequent diagnoses, while in children, minimal change disease and focal segmental glomerulosclerosis were the most common.</div></div><div><h3>Conclusions</h3><div>The type of glomerular disease varies according to sociodemographic factors such as age and sex. Lupus nephritis and focal segmental glomerulosclerosis were the most common glomerular diseases. The main indications for biopsy were nephrotic syndrome and abnormal urinary sediment.</div></div>","PeriodicalId":39194,"journal":{"name":"Revista Espanola de Patologia","volume":"58 3","pages":"Article 100813"},"PeriodicalIF":0.0,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143477985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mucopolysaccharidoses type VII (Sly syndrome): New uncertain pathogenic variants in GUSB gene

Revista Espanola de Patologia Pub Date : 2025-02-20 DOI: 10.1016/j.patol.2025.100814

Emilio I. Abecia Martínez , Jorge Puente Prieto , Jorge Luis Delgado Fernández , Javier Martínez Castillón , Carlos Nagore González , Lucia Ferrando Lamana

{"title":"Mucopolysaccharidoses type VII (Sly syndrome): New uncertain pathogenic variants in GUSB gene","authors":"Emilio I. Abecia Martínez , Jorge Puente Prieto , Jorge Luis Delgado Fernández , Javier Martínez Castillón , Carlos Nagore González , Lucia Ferrando Lamana","doi":"10.1016/j.patol.2025.100814","DOIUrl":"10.1016/j.patol.2025.100814","url":null,"abstract":"<div><div>Mucopolysaccharidoses (MPS) are a group of diseases caused by the accumulation of glycosaminoglycans (GAGs). Currently, eleven enzyme deficiencies have been described, with eight different MPS subtypes reported. MPS VII, also known as Sly syndrome, is a recessive disorder caused by pathogenic variants in the <em>GUSB</em> gene. This results in dysfunction of the enzyme β-glucuronidase, which can lead to non-immune hydrops fetalis (NIHF). The patient was a 22-week foetus with pathological nuchal translucency and unfavourable prognosis based on radiological criteria; as a result, both parents opted to terminate the pregnancy. Post mortem examination revealed several hallmarks of NIHF. Moreover, microscopic examination showed widespread histiocytes with microvacuolated cytoplasm. Genetic testing demonstrated biallelic variants in exon 8 of the <em>GUSB</em> gene, but both of uncertain meaning. Subsequent histological findings suggested that these <em>GUSB</em> gene variants resulted in MPS VII.</div></div>","PeriodicalId":39194,"journal":{"name":"Revista Espanola de Patologia","volume":"58 3","pages":"Article 100814"},"PeriodicalIF":0.0,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143444790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Parotid rhabdomyosarcoma with spindle cell features masquerading as a benign salivary gland tumour: Pitfall alert

Revista Espanola de Patologia Pub Date : 2025-02-20 DOI: 10.1016/j.patol.2025.100808

Thotadamane Nagaraja Chandrashekhar , Apoorva Narayana Reddy , Priyadharshini Bargunam , Saba Afreen Raheem , Ragashree Apparasanahalli Siddalingamurthy

{"title":"Parotid rhabdomyosarcoma with spindle cell features masquerading as a benign salivary gland tumour: Pitfall alert","authors":"Thotadamane Nagaraja Chandrashekhar , Apoorva Narayana Reddy , Priyadharshini Bargunam , Saba Afreen Raheem , Ragashree Apparasanahalli Siddalingamurthy","doi":"10.1016/j.patol.2025.100808","DOIUrl":"10.1016/j.patol.2025.100808","url":null,"abstract":"<div><div>Rhabdomyosarcoma (RMS) is the most common sarcoma in children and adolescents, with the head and neck region, extremities, and genitourinary tract being the most common locations. Rhabdomyosarcoma of the salivary glands is rarely seen in children and can easily be mistaken for their benign counterparts. We report a case of rhabdomyosarcoma in the parotid region that clinically and cytologically masqueraded as a benign parotid gland tumour, leading to diagnostic difficulties. However, the excised specimen revealed histomorphological and immunophenotypic features consistent with rhabdomyosarcoma. The child underwent a total parotidectomy followed by chemotherapy and is doing well without recurrence. Although cytology is minimally invasive and has good specificity, there are rare cases, such as this one, where histopathology provided the definitive diagnosis. This report aims to raise awareness of RMS of the parotid gland among clinicians, cytopathologists, and radiologists, aiding in the early diagnosis and treatment of these cases.</div></div>","PeriodicalId":39194,"journal":{"name":"Revista Espanola de Patologia","volume":"58 2","pages":"Article 100808"},"PeriodicalIF":0.0,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143445285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Peritoneal carcinomatosis after lumboperitoneal shunt in a patient with a cancer of unknown primary site: Case report and review of the literature

Revista Espanola de Patologia Pub Date : 2025-02-20 DOI: 10.1016/j.patol.2025.100802

Miquel Piñol Ribas , Robert Begué , Víctor Martínez , Elena García Alonso , Santiago Miguelsanz , Sònia Gatius

{"title":"Peritoneal carcinomatosis after lumboperitoneal shunt in a patient with a cancer of unknown primary site: Case report and review of the literature","authors":"Miquel Piñol Ribas , Robert Begué , Víctor Martínez , Elena García Alonso , Santiago Miguelsanz , Sònia Gatius","doi":"10.1016/j.patol.2025.100802","DOIUrl":"10.1016/j.patol.2025.100802","url":null,"abstract":"<div><div>Cancer of unknown primary site (CUP) often presents with atypical clinical behaviours that make identifying the primary tumour challenging. In this paper, we discuss the case of a patient with brain metastases from a CUP, which was ultimately diagnosed as pleomorphic carcinoma of the lung following autopsy. Clinically, the development of peritoneal carcinomatosis had reduced suspicion of a primary lung neoplasm, despite imaging tests initially suggesting this. However, the patient had previously undergone lumboperitoneal (LP) shunt insertion. The sequence of clinical events and autopsy findings strongly indicated that the LP shunt facilitated the metastatic spread.</div><div>In addition to this case report, we provide a systematic review of the literature on the role of ventriculoperitoneal (VP) and LP shunts in the metastatic dissemination of tumours. Although the spread of primary brain tumours via VP or LP shunts is rare, albeit well-documented, this phenomenon has been reported only twice for secondary tumours.</div></div>","PeriodicalId":39194,"journal":{"name":"Revista Espanola de Patologia","volume":"58 2","pages":"Article 100802"},"PeriodicalIF":0.0,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143445282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0