Enzymes Pub Date : 2023-01-01 Epub Date: 2023-06-03 DOI: 10.1016/bs.enz.2023.05.002

Feng He, Marco Bravo, Li Fan

引用次数: 0

Regulatory miRNAs in cancer cell recovery from therapy exposure and its implications as a novel therapeutic strategy for preventing disease recurrence. 癌症细胞从治疗暴露中恢复的调节性miRNA及其作为预防疾病复发的新治疗策略的意义。

Enzymes Pub Date : 2023-01-01 Epub Date: 2023-08-30 DOI: 10.1016/bs.enz.2023.07.007

Joseph Landry, Kathryn Shows, Akash Jagdeesh, Aashka Shah, Mihir Pokhriyal, Vasily Yakovlev

{"title":"Regulatory miRNAs in cancer cell recovery from therapy exposure and its implications as a novel therapeutic strategy for preventing disease recurrence.","authors":"Joseph Landry, Kathryn Shows, Akash Jagdeesh, Aashka Shah, Mihir Pokhriyal, Vasily Yakovlev","doi":"10.1016/bs.enz.2023.07.007","DOIUrl":"10.1016/bs.enz.2023.07.007","url":null,"abstract":"The desired outcome of cancer therapies is the eradication of disease. This can be achieved when therapy exposure leads to therapy-induced cancer cell death as the dominant outcome. Theoretically, a permanent therapy-induced growth arrest could also contribute to a complete response, which has the potential to lead to remission. However, preclinical models have shown that therapy-induced growth arrest is not always durable, as recovering cancer cell populations can contribute to the recurrence of cancer. Significant research efforts have been expended to develop strategies focusing on the prevention of recurrence. Recovery of cells from therapy exposure can occur as a result of several cell stress adaptations. These include cytoprotective autophagy, cellular quiescence, a reversable form of senescence, and the suppression of apoptosis and necroptosis. It is well documented that microRNAs regulate the response of cancer cells to anti-cancer therapies, making targeting microRNAs therapeutically a viable strategy to sensitization and the prevention of recovery. We propose that the use of microRNA-targeting therapies in prolonged sequence, that is, a significant period after initial therapy exposure, could reduce toxicity from the standard combination strategy, and could exploit new epigenetic states essential for cancer cells to recover from therapy exposure. In a step toward supporting this strategy, we survey the available scientific literature to identify microRNAs which could be targeted in sequence to eliminate residual cancer cell populations that were arrested as a result of therapy exposure. It is our hope that by successfully identifying microRNAs which could be targeted in sequence we can prevent disease recurrence.","PeriodicalId":39097,"journal":{"name":"Enzymes","volume":"53 ","pages":"113-196"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41132747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preface. 前言

Enzymes Pub Date : 2023-01-01 DOI: 10.1016/S1874-6047(23)00025-2

David N Arnosti, Laurie S Kaguni

引用次数: 0

Soft repression and chromatin modification by conserved transcriptional corepressors. 保守转录辅压子的软抑制和染色质修饰。

Enzymes Pub Date : 2023-01-01 Epub Date: 2023-09-14 DOI: 10.1016/bs.enz.2023.08.001

David N Arnosti

引用次数: 0

DNA replication machineries: Structural insights from crystallography and electron microscopy. DNA复制机制：结晶学和电子显微镜的结构见解。

Enzymes Pub Date : 2023-01-01 Epub Date: 2023-07-27 DOI: 10.1016/bs.enz.2023.07.004

Rebeca Bocanegra, María Ortíz-Rodríguez, Lyra Zumeta, Ismael Plaza-G A, Elías Faro, Borja Ibarra

引用次数: 0

The mitochondrial respiratory chain. 线粒体呼吸链。

Enzymes Pub Date : 2023-01-01 Epub Date: 2023-05-26 DOI: 10.1016/bs.enz.2023.05.001

Mårten Wikström, Cristina Pecorilla, Vivek Sharma

引用次数: 0

Mitochondrial AAA+ proteases. 线粒体AAA+蛋白酶。

Enzymes Pub Date : 2023-01-01 Epub Date: 2023-10-13 DOI: 10.1016/bs.enz.2023.09.002

Yuichi Matsushima

引用次数: 0

Sphingolipids: From structural components to signaling hubs. 鞘磷脂：从结构成分到信号中枢。

Enzymes Pub Date : 2023-01-01 Epub Date: 2023-08-28 DOI: 10.1016/bs.enz.2023.07.003

Batoul M Issleny, Rama Jamjoum, Saurav Majumder, Johnny Stiban

{"title":"Sphingolipids: From structural components to signaling hubs.","authors":"Batoul M Issleny, Rama Jamjoum, Saurav Majumder, Johnny Stiban","doi":"10.1016/bs.enz.2023.07.003","DOIUrl":"10.1016/bs.enz.2023.07.003","url":null,"abstract":"In late November 2019, Prof. Lina M. Obeid passed away from cancer, a disease she spent her life researching and studying its intricate molecular underpinnings. Along with her husband, Prof. Yusuf A. Hannun, Obeid laid down the foundations of sphingolipid biochemistry and oversaw its remarkable evolution over the years. Lipids are a class of macromolecules that are primarily associated with cellular architecture. In fact, lipids constitute the perimeter of the cell in such a way that without them, there cannot be cells. Hence, much of the early research on lipids identified the function of this class of biological molecules as merely structural. Nevertheless, unlike proteins, carbohydrates, and nucleic acids, lipids are elaborately diverse as they are not made up of monomers in polymeric forms. This diversity in structure is clearly mirrored by functional pleiotropy. In this chapter, we focus on a major subset of lipids, sphingolipids, and explore their historic rise from merely inert structural components of plasma membranes to lively and necessary signaling molecules that transmit various signals and control many cellular processes. We will emphasize the works of Lina Obeid since she was an integral pillar of the sphingolipid research world.","PeriodicalId":39097,"journal":{"name":"Enzymes","volume":"54 ","pages":"171-201"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72015667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Conserved mechanisms of NuRD function in hematopoetic gene expression. NuRD在造血基因表达中功能的保守机制。

Enzymes Pub Date : 2023-01-01 Epub Date: 2023-08-30 DOI: 10.1016/bs.enz.2023.07.006

Jonathan Lenz, Alexander Brehm

{"title":"Conserved mechanisms of NuRD function in hematopoetic gene expression.","authors":"Jonathan Lenz, Alexander Brehm","doi":"10.1016/bs.enz.2023.07.006","DOIUrl":"https://doi.org/10.1016/bs.enz.2023.07.006","url":null,"abstract":"The Nucleosome Remodeling and Deacetylating Complex (NuRD) is ubiquitously expressed in all metazoans. It combines nucleosome remodeling and histone deacetylating activities to generate inaccessible chromatin structures and to repress gene transcription. NuRD is involved in the generation and maintenance of a wide variety of lineage-specific gene expression programs during differentiation and in differentiated cells. A close cooperation with a large number of lineage-specific transcription factors is key to allow NuRD to function in many distinct differentiation contexts. The molecular nature of this interplay between transcription factors and NuRD is complex and not well understood. This review uses hematopoiesis as a paradigm to highlight recent advances in our understanding of how transcription factors and NuRD cooperate at the molecular level during differentiation. A comparison of vertebrate and invertebrate systems serves to identify the conserved and fundamental concepts guiding functional interactions between transcription factors and NuRD. We also discuss how the transcription factor-NuRD axis constitutes a potential therapeutic target for the treatment of hemoglobinopathies.","PeriodicalId":39097,"journal":{"name":"Enzymes","volume":"53 ","pages":"7-32"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41146415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

History of advances in enzyme kinetic methods: From minutes to milliseconds. 酶动力学方法的发展史：从几分钟到几毫秒。

Enzymes Pub Date : 2023-01-01 Epub Date: 2023-08-08 DOI: 10.1016/bs.enz.2023.07.005

Kenneth A Johnson

{"title":"History of advances in enzyme kinetic methods: From minutes to milliseconds.","authors":"Kenneth A Johnson","doi":"10.1016/bs.enz.2023.07.005","DOIUrl":"10.1016/bs.enz.2023.07.005","url":null,"abstract":"The last review on transient-state kinetic methods in The Enzymes was published three decades ago (Johnson, K.A., 1992. The Enzymes, XX, 1-61). In that review the foundations were laid out for the logic behind the design and interpretation of experiments. In the intervening years the instrumentation has improved mainly in providing better sample economy and shorter dead times. More significantly, in 1992 we were just introducing methods for fitting data based on numerical integration of rate equations, but the software was slow and difficult to use. Today, advances in numerical integration methods for data fitting have led to fast and dynamic software, making it easy to fit data without simplifying approximations. This approach overcomes multiple disadvantages of traditional data fitting based on equations derived by analytical integration of rate equations, requiring simplifying approximations. Mechanism-based fitting using computer simulation resolves mechanisms by accounting for the concentration dependence of the rates and amplitudes of the reaction to find a set of intrinsic rate constants that reproduce the experimental data, including details about how the experiment was performed in modeling the data. Rather than discuss how to design and interpret rapid-quench and stopped-flow experiments individually, we now focus on how to fit them simultaneously so that the quench-flow data anchor the interpretation of fluorescence signals. Computer simulation streamlines the fitting of multiple experiments globally to yield a single unifying model to account for all available data.","PeriodicalId":39097,"journal":{"name":"Enzymes","volume":"54 ","pages":"107-134"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72015663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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