International Journal of Cell Biology最新文献_第5页

Odontoblast-Like Cells Differentiated from Dental Pulp Stem Cells Retain Their Phenotype after Subcultivation. 牙髓干细胞分化成成牙髓细胞样细胞经继代培养后保持其表型。

International Journal of Cell Biology Pub Date : 2018-02-19 eCollection Date: 2018-01-01 DOI: 10.1155/2018/6853189

Paula A Baldión, Myriam L Velandia-Romero, Jaime E Castellanos

{"title":"Odontoblast-Like Cells Differentiated from Dental Pulp Stem Cells Retain Their Phenotype after Subcultivation.","authors":"Paula A Baldión, Myriam L Velandia-Romero, Jaime E Castellanos","doi":"10.1155/2018/6853189","DOIUrl":"https://doi.org/10.1155/2018/6853189","url":null,"abstract":"Odontoblasts, the main cell type in teeth pulp tissue, are not cultivable and they are responsible for the first line of response after dental restauration. Studies on dental materials cytotoxicity and odontoblast cells physiology require large quantity of homogenous cells retaining most of the phenotype characteristics. Odontoblast-like cells (OLC) were differentiated from human dental pulp stem cells using differentiation medium (containing TGF-β1), and OLC expanded after trypsinization (EXP-21) were evaluated and compared. Despite a slower cell growth curve, EXP-21 cells express similarly the odontoblast markers dentinal sialophosphoprotein and dentin matrix protein-1 concomitantly with RUNX2 transcripts and low alkaline phosphatase activity as expected. Both OLC and EXP-21 cells showed similar mineral deposition activity evidenced by alizarin red and von Kossa staining. These results pointed out minor changes in phenotype of subcultured EXP-21 regarding the primarily differentiated OLC, making the subcultivation of these cells a useful strategy to obtain odontoblasts for biocompatibility or cell physiology studies in dentistry.","PeriodicalId":39084,"journal":{"name":"International Journal of Cell Biology","volume":"2018 ","pages":"6853189"},"PeriodicalIF":0.0,"publicationDate":"2018-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2018/6853189","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36021674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 46

Thrombin-Induced Calpain Activation Promotes Protease-Activated Receptor 1 Internalization. 凝血酶诱导的钙蛋白酶激活促进蛋白酶激活受体1内化。

International Journal of Cell Biology Pub Date : 2017-01-01 Epub Date: 2017-11-09 DOI: 10.1155/2017/1908310

Alejandro Alvarez-Arce, Irene Lee-Rivera, Edith López, Arturo Hernández-Cruz, Ana María López-Colomé

{"title":"Thrombin-Induced Calpain Activation Promotes Protease-Activated Receptor 1 Internalization.","authors":"Alejandro Alvarez-Arce, Irene Lee-Rivera, Edith López, Arturo Hernández-Cruz, Ana María López-Colomé","doi":"10.1155/2017/1908310","DOIUrl":"https://doi.org/10.1155/2017/1908310","url":null,"abstract":"The serine protease thrombin activates Protease-Activated Receptors (PARs), a family of G-protein-coupled receptors (GPCRs) activated by the proteolytic cleavage of their extracellular N-terminal domain. Four members of this family have been identified: PAR1-4. The activation of Protease-Activated Receptor 1(PAR1), the prototype of this receptor family, leads to an increase in intracellular Ca+2 concentration ([Ca+2]i) mediated by Gq11α coupling and phospholipase C (PLC) activation. We have previously shown that the stimulation of PAR1 by thrombin promotes intracellular signaling leading to RPE cell transformation, proliferation, and migration which characterize fibroproliferative eye diseases leading to blindness. Within this context, the elucidation of the mechanisms involved in PAR1 inactivation is of utmost importance. Due to the irreversible nature of PAR1 activation, its inactivation must be efficiently regulated in order to terminate signaling. Using ARPE-19 human RPE cell line, we characterized thrombin-induced [Ca+2]i increase and demonstrated the calcium-dependent activation of μ-calpain mediated by PAR1. Calpains are a family of calcium-activated cysteine proteases involved in multiple cellular processes including the internalization of membrane proteins through clathrin-coated vesicles. We demonstrated that PAR1-induced calpain activation results in the degradation of α-spectrin by calpain, essential for receptor endocytosis, and the consequent decrease in PAR1 membrane expression. Collectively, the present results identify a novel μ-calpain-dependent mechanism for PAR1 inactivation following exposure to thrombin.","PeriodicalId":39084,"journal":{"name":"International Journal of Cell Biology","volume":"2017 ","pages":"1908310"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2017/1908310","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35664146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Class-Specific Histone Deacetylase Inhibitors Promote 11-Beta Hydroxysteroid Dehydrogenase Type 2 Expression in JEG-3 Cells. 类特异性组蛋白去乙酰化酶抑制剂促进JEG-3细胞中11- β羟基类固醇脱氢酶2型的表达。

International Journal of Cell Biology Pub Date : 2017-01-01 Epub Date: 2017-02-21 DOI: 10.1155/2017/6169310

Katie L Togher, Louise C Kenny, Gerard W O'Keeffe

{"title":"Class-Specific Histone Deacetylase Inhibitors Promote 11-Beta Hydroxysteroid Dehydrogenase Type 2 Expression in JEG-3 Cells.","authors":"Katie L Togher, Louise C Kenny, Gerard W O'Keeffe","doi":"10.1155/2017/6169310","DOIUrl":"https://doi.org/10.1155/2017/6169310","url":null,"abstract":"Exposure to maternal cortisol plays a crucial role in fetal organogenesis. However, fetal overexposure to cortisol has been linked to a range of short- and long-term adverse outcomes. Normally, this is prevented by the expression of an enzyme in the placenta called 11-beta hydroxysteroid dehydrogenase type 2 (11β-HSD2) which converts active cortisol to its inactive metabolite cortisone. Placental 11β-HSD2 is known to be reduced in a number of adverse pregnancy complications, possibly through an epigenetic mechanism. As a result, a number of pan-HDAC inhibitors have been examined for their ability to promote 11β-HSD2 expression. However, it is not known if the effects of pan-HDAC inhibition are a general phenomenon or if the effects are dependent upon a specific class of HDACs. Here, we examined the ability of pan- and class-specific HDAC inhibitors to regulate 11β-HSD2 expression in JEG3 cells. We find that pan-, class I, or class IIa HDAC inhibition promoted 11β-HSD2 expression and prevented cortisol or interleukin-1β-induced decrease in its expression. These results demonstrate that targeting a specific class of HDACs can promote 11β-HSD2 expression in JEG3 cells. This adds to the growing body of evidence suggesting that HDACs may be crucial in maintaining normal fetal development.","PeriodicalId":39084,"journal":{"name":"International Journal of Cell Biology","volume":"2017 ","pages":"6169310"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2017/6169310","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34837099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Thrombopoietin Secretion by Human Ovarian Cancer Cells. 人卵巢癌细胞的血小板生成素分泌。

International Journal of Cell Biology Pub Date : 2017-01-01 Epub Date: 2017-03-30 DOI: 10.1155/2017/1873834

Samaher Besbes, Shahid Shah, Iman Al-Dybiat, Shahsoltan Mirshahi, Helene Helfer, Haythem Najah, Caroline Fourgeaud, Marc Pocard, Ibtissem Ghedira, Jeannette Soria, Massoud Mirshahi

{"title":"Thrombopoietin Secretion by Human Ovarian Cancer Cells.","authors":"Samaher Besbes, Shahid Shah, Iman Al-Dybiat, Shahsoltan Mirshahi, Helene Helfer, Haythem Najah, Caroline Fourgeaud, Marc Pocard, Ibtissem Ghedira, Jeannette Soria, Massoud Mirshahi","doi":"10.1155/2017/1873834","DOIUrl":"https://doi.org/10.1155/2017/1873834","url":null,"abstract":"The thrombopoietin (TPO) gene expression in human ovary and cancer cells from patients with ovarian carcinomatosis, as well as several cancer cell lines including MDA-MB231 (breast cancer), K562 and HL60 (Leukemic cells), OVCAR-3NIH and SKOV-3 (ovarian cancer), was performed using RT PCR, real-time PCR, and gene sequencing. Human liver tissues are used as controls. The presence of TPO in the cells and its regulation by activated protein C were explored by flow cytometry. TPO content of cell extract as well as plasma of a patient with ovarian cancer was evaluated by ELISA. The functionality of TPO was performed in coculture on the basis of the viability of a TPO-dependent cell line (Ba/F3), MTT assay, and Annexin-V labeling. As in liver, ovarian tissues and all cancer cells lines except the MDA-MB231 express the three TPO-1 (full length TPO), TPO-2 (12 bp deletion), and TPO-3 (116 pb deletion) variants. Primary ovarian cancer cells as well as cancer cell lines produce TPO. The thrombopoietin production by OVCAR-3 increased when cells are stimulated by aPC. OVCAR-3 cell's supernatant can replace exogenous TPO and inhibited TPO-dependent cell line (Ba/F3) apoptosis. The thrombopoietin produced by tumor may have a direct effect on thrombocytosis/thrombosis occurrence in patients with ovarian cancer.","PeriodicalId":39084,"journal":{"name":"International Journal of Cell Biology","volume":"2017 ","pages":"1873834"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2017/1873834","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34961328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Nesprin-2 Interacts with Condensin Component SMC2. nesprin2与冷凝素组件SMC2相互作用。

International Journal of Cell Biology Pub Date : 2017-01-01 Epub Date: 2017-12-27 DOI: 10.1155/2017/8607532

Xin Xing, Carmen Mroß, Linlin Hao, Martina Munck, Alexandra Herzog, Clara Mohr, C P Unnikannan, Pranav Kelkar, Angelika A Noegel, Ludwig Eichinger, Sascha Neumann

{"title":"Nesprin-2 Interacts with Condensin Component SMC2.","authors":"Xin Xing, Carmen Mroß, Linlin Hao, Martina Munck, Alexandra Herzog, Clara Mohr, C P Unnikannan, Pranav Kelkar, Angelika A Noegel, Ludwig Eichinger, Sascha Neumann","doi":"10.1155/2017/8607532","DOIUrl":"https://doi.org/10.1155/2017/8607532","url":null,"abstract":"The nuclear envelope proteins, Nesprins, have been primarily studied during interphase where they function in maintaining nuclear shape, size, and positioning. We analyze here the function of Nesprin-2 in chromatin interactions in interphase and dividing cells. We characterize a region in the rod domain of Nesprin-2 that is predicted as SMC domain (aa 1436-1766). We show that this domain can interact with itself. It furthermore has the capacity to bind to SMC2 and SMC4, the core subunits of condensin. The interaction was observed during all phases of the cell cycle; it was particularly strong during S phase and persisted also during mitosis. Nesprin-2 knockdown did not affect condensin distribution; however we noticed significantly higher numbers of chromatin bridges in Nesprin-2 knockdown cells in anaphase. Thus, Nesprin-2 may have an impact on chromosomes which might be due to its interaction with condensins or to indirect mechanisms provided by its interactions at the nuclear envelope.","PeriodicalId":39084,"journal":{"name":"International Journal of Cell Biology","volume":"2017 ","pages":"8607532"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2017/8607532","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35831491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Regeneration and Regrowth Potentials of Digit Tips in Amphibians and Mammals. 两栖动物和哺乳动物趾尖的再生和再生潜力。

International Journal of Cell Biology Pub Date : 2017-01-01 Epub Date: 2017-04-10 DOI: 10.1155/2017/5312951

Yohan Choi, Fanwei Meng, Charles S Cox, Kevin P Lally, Johnny Huard, Yong Li

引用次数: 11

Effect of Antioxidant Water on the Bioactivities of Cells. 抗氧化水对细胞生物活性的影响。

International Journal of Cell Biology Pub Date : 2017-01-01 Epub Date: 2017-08-17 DOI: 10.1155/2017/1917239

Seong Gu Hwang, Ho-Sung Lee, Byung-Cheon Lee, GunWoong Bahng

{"title":"Effect of Antioxidant Water on the Bioactivities of Cells.","authors":"Seong Gu Hwang, Ho-Sung Lee, Byung-Cheon Lee, GunWoong Bahng","doi":"10.1155/2017/1917239","DOIUrl":"https://doi.org/10.1155/2017/1917239","url":null,"abstract":"It has been reported that water at the interface of a hydrophilic thin film forms an exclusion zone, which has a higher density than ordinary water. A similar phenomenon was observed for a hydrated hydrophilic ceramic powder, and water turns into a three-dimensional cell-like structure composed of high density water and low density water. This structured water appears to have a stimulative effect on plant growth. This report outlines our study of antioxidant properties of this structured water and its effect on cell bioactivities. Culturing media which were prepared utilizing this antioxidant structured water promoted the viability of RAW 264.7 macrophage cells by up to three times. The same tendency was observed for other cells including IEC-6, C2C12, and 3T3-L1. Also, the cytokine expression of the splenocytes taken from a mouse spleen increased in the same manner. The water also appears to suppress the viability of cancer cell, MCF-7. These results strongly suggest that the structured water helps the activities of normal cells while suppressing those of malignant cells.","PeriodicalId":39084,"journal":{"name":"International Journal of Cell Biology","volume":"2017 ","pages":"1917239"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2017/1917239","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35407332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Pulling a Ligase out of a "HAT": pCAF Mediates Ubiquitination of the Class II Transactivator. 从“HAT”中拔出连接酶:pCAF介导II类反激活子的泛素化。

International Journal of Cell Biology Pub Date : 2017-01-01 Epub Date: 2017-02-12 DOI: 10.1155/2017/8093813

Julie E Morgan, Susanna F Greer

{"title":"Pulling a Ligase out of a \"HAT\": pCAF Mediates Ubiquitination of the Class II Transactivator.","authors":"Julie E Morgan, Susanna F Greer","doi":"10.1155/2017/8093813","DOIUrl":"https://doi.org/10.1155/2017/8093813","url":null,"abstract":"The Class II Transactivator (CIITA) is essential to the regulation of Major Histocompatibility Class II (MHC II) genes transcription. As the \"master regulator\" of MHC II transcription, CIITA regulation is imperative and requires various posttranslational modifications (PTMs) in order to facilitate its role. Previously we identified various ubiquitination events on CIITA. Monoubiquitination is important for CIITA transactivity, while K63 linked ubiquitination is involved in crosstalk with ERK1/2 phosphorylation, where together they mediate cellular movement from the cytoplasm to nuclear region. Further, CIITA is also modified by degradative K48 polyubiquitination. However, the E3 ligase responsible for these modifications was unknown. We show CIITA ubiquitination and transactivity are enhanced with the histone acetyltransferase (HAT), p300/CBP associated factor (pCAF), and the E3 ligase region within pCAF is necessary for both. Additionally, pCAF mediated ubiquitination is independent of pCAF's HAT domain, and acetylation deficient CIITA is K48 polyubiquitinated and degraded in the presence of pCAF. Lastly, we identify the histone acetyltransferase, pCAF, as the E3 ligase responsible for CIITA's ubiquitination.","PeriodicalId":39084,"journal":{"name":"International Journal of Cell Biology","volume":"2017 ","pages":"8093813"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2017/8093813","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34805956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Biologics for Targeting Inflammatory Cytokines, Clinical Uses, and Limitations 靶向炎性细胞因子的生物制剂，临床应用和局限性

International Journal of Cell Biology Pub Date : 2016-12-19 DOI: 10.1155/2016/9259646

Peleg Rider, Y. Carmi, Idan Cohen

{"title":"Biologics for Targeting Inflammatory Cytokines, Clinical Uses, and Limitations","authors":"Peleg Rider, Y. Carmi, Idan Cohen","doi":"10.1155/2016/9259646","DOIUrl":"https://doi.org/10.1155/2016/9259646","url":null,"abstract":"Proinflammatory cytokines are potent mediators of numerous biological processes and are tightly regulated in the body. Chronic uncontrolled levels of such cytokines can initiate and derive many pathologies, including incidences of autoimmunity and cancer. Therefore, therapies that regulate the activity of inflammatory cytokines, either by supplementation of anti-inflammatory recombinant cytokines or by neutralizing them by using blocking antibodies, have been extensively used over the past decades. Over the past few years, new innovative biological agents for blocking and regulating cytokine activities have emerged. Here, we review some of the most recent approaches of cytokine targeting, focusing on anti-TNF antibodies or recombinant TNF decoy receptor, recombinant IL-1 receptor antagonist (IL-1Ra) and anti-IL-1 antibodies, anti-IL-6 receptor antibodies, and TH17 targeting antibodies. We discuss their effects as biologic drugs, as evaluated in numerous clinical trials, and highlight their therapeutic potential as well as emphasize their inherent limitations and clinical risks. We suggest that while systemic blocking of proinflammatory cytokines using biological agents can ameliorate disease pathogenesis and progression, it may also abrogate the hosts defense against infections. Moreover, we outline the rational need to develop new therapies, which block inflammatory cytokines only at sites of inflammation, while enabling their function systemically.","PeriodicalId":39084,"journal":{"name":"International Journal of Cell Biology","volume":"2016 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2016/9259646","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"64603968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 151

Localisation of Lactate Transporters in Rat and Rabbit Placentae 乳酸转运蛋白在大鼠和兔胎盘中的定位

International Journal of Cell Biology Pub Date : 2016-10-23 DOI: 10.1155/2016/2084252

N. Moore, C. Picut, J. Charlap

{"title":"Localisation of Lactate Transporters in Rat and Rabbit Placentae","authors":"N. Moore, C. Picut, J. Charlap","doi":"10.1155/2016/2084252","DOIUrl":"https://doi.org/10.1155/2016/2084252","url":null,"abstract":"The distribution of monocarboxylate transporter (MCT) isoforms 1 and 4, which mediate the plasmalemmal transport of l-lactic and pyruvic acids, has been identified in the placentae of rats and rabbits at different ages of gestation. Groups of three pregnant Sprague-Dawley rats and New Zealand White rabbits were sacrificed on gestation days (GD) 11, 14, 18, or 20 and on GD 13, 18, or 28, respectively. Placentae were removed and processed for immunohistochemical detection of MCT1 and MCT4. In the rat, staining for MCT1 was associated with lakes and blood vessels containing enucleated red blood cells (maternal vessels) while staining for MCT4 was associated with vessels containing nucleated red blood cells (embryofoetal vessels). In the rabbit, staining for MCT1 was associated with blood vessels containing nucleated red blood cells while staining for MCT4 was associated with vessels containing enucleated red blood cells. Strength of staining for MCT1 decreased during gestation in both species, but that for MCT4 was stronger than that for MCT1 and was consistent between gestation days. The results imply an opposite polarity of MCT1 and MCT4 across the trophoblast between rat and rabbit.","PeriodicalId":39084,"journal":{"name":"International Journal of Cell Biology","volume":"2016 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2016/2084252","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"64260688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6